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Hamel Mohaisen, S, Hussain Ali, M, Hashem Shehab, Z, Al-Mayyahi, A. W, Abdulhassan, A. A. (1400). Effect of Ultraviolet Light on the Expression of icaD Gene in Staphylococcus aureus Local Isolates in Iraq. سامانه مدیریت نشریات علمی, 76(5), 1221-1227. doi: 10.22092/ari.2021.355654.1706
S Hamel Mohaisen; M Hussain Ali; Z Hashem Shehab; A. W Al-Mayyahi; A. A Abdulhassan. "Effect of Ultraviolet Light on the Expression of icaD Gene in Staphylococcus aureus Local Isolates in Iraq". سامانه مدیریت نشریات علمی, 76, 5, 1400, 1221-1227. doi: 10.22092/ari.2021.355654.1706
Hamel Mohaisen, S, Hussain Ali, M, Hashem Shehab, Z, Al-Mayyahi, A. W, Abdulhassan, A. A. (1400). 'Effect of Ultraviolet Light on the Expression of icaD Gene in Staphylococcus aureus Local Isolates in Iraq', سامانه مدیریت نشریات علمی, 76(5), pp. 1221-1227. doi: 10.22092/ari.2021.355654.1706
Hamel Mohaisen, S, Hussain Ali, M, Hashem Shehab, Z, Al-Mayyahi, A. W, Abdulhassan, A. A. Effect of Ultraviolet Light on the Expression of icaD Gene in Staphylococcus aureus Local Isolates in Iraq. سامانه مدیریت نشریات علمی, 1400; 76(5): 1221-1227. doi: 10.22092/ari.2021.355654.1706

Effect of Ultraviolet Light on the Expression of icaD Gene in Staphylococcus aureus Local Isolates in Iraq

Archives of Razi Institute
مقاله 10، دوره 76، شماره 5، بهمن و اسفند 2021، صفحه 1221-1227    اصل مقاله (532.24 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355654.1706
نویسندگان
S Hamel Mohaisen* 1؛ M Hussain Ali1؛ Z Hashem Shehab2؛ A. W Al-Mayyahi3؛ A. A Abdulhassan1
1Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad, Iraq
2Biology Department, College of Science for Women, University of Baghdad, Baghdad, Iraq
3Employee in the Ministry of Health, Iraq
چکیده
The current study was designed to reveal the impact of ultraviolet (UV) light on polysaccharide intercellular adhesion of the icaD gene expression and its relation to biofilm as virulence markers in Staphylococcus aureus. In total, 37 isolates of S. aureus were gathered from various bacterial infection sources, and 26 S. aureus isolates were definite to icaD gene using the Polymerase Chain Reaction (PCR) technique. Prior to and after exposure to UV light, RNAs from the isolates were extracted which had strong biofilm formation by crystal violet staining assay, and they were then exposed to quantitative real-time PCR (qPCR).  In vitro, the survival of isolates was evaluated after the exposition to several periods of the UV light power via counting the number of colonies. The results showed that the exposure to the UV light at 320 nm for 2, 4, 6, 8, and 10 min had inhibitory effects on the bacterial growth. The sub-lethal exposure time was 8 min. The rapid reduction of bacterial survival rates was associated with the increment irradiation time of UV power from 5 to 1.24 log10 CFU/ml.  In general, down regulation of the icaD gene expression was decreased upon exposure to the UV light which was used as a physical agent for controlling the bacterial infection and biofilm formation.
کلیدواژه‌ها
Staphylococcus aureus؛ UV light؛ icaD gene؛ real-time PCR
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مراجع
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