Related Links
In vitro propagation of marigold (Silybum marianum L.) via direct regeneration | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| Article 7, Volume 37, Issue 4 - Serial Number 108, September and October 2021, Pages 628-638 PDF (872.42 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijmapr.2021.353856.2956 | ||
| Authors | ||
| F. Noormand Moaied* 1; N. Valizadeh2; T. Samandari Giklo3 | ||
| 1Research Division of Natural Resources, East Azarbaijan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extention Organization (AREEO), Tabriz, Iran | ||
| 2Ph.D. graduate, Medicinal Plants and By-Products Research Department, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extention Organization (AREEO), Tehran, Iran | ||
| 3- Ph.D. graduate, Agricultural Services Management of Parsabad (Moghan), Agricultural Jihad Organization of Ardabil Province, Iran | ||
| Abstract | ||
| Silybum marianum L., from fam. asteraceae, is a very important medicinal plant in the pharmaceutical industry due to its active ingredient silymarin. Silymarin is very effective in treating the liver diseases, hepatitis, and cardiovascular disorders. Since the potential and production speed of this medicinal compound is low in habitats under natural conditions, the use of genetic engineering and production of transgenic plants using the various techniques such as tissue culture has been considered in this plant. The first step in this direction is to obtain an in vitro regeneration protocol. In this study, the leaf explants obtained from the Hungarian cultivar of S. marianum were evaluated for the direct regeneration affected by different combinations of hormones. Six weeks after the culture, the highest shooting (57%) was observed in the ½ MS medium containing 0.4 mg l-1 zeatin, 0.2 mg l-1 BAP, and 0.2 mg l-1 NAA. The shoots were then transferred to the root culture medium with the different hormonal treatments. The results showed that the best treatment to obtain the long and high roots was the ½ MS medium containing 0.25 mg l-1 NAA and 2 g l-1 activated charcoal. The seedlings obtained from the direct regeneration grew well after transplanting into the soil. | ||
| Keywords | ||
| Regeneration; Milk thistle (Silybum marianum L.); hormone; silymarin; shooting; rooting | ||
| References | ||
|
- Abbasi, B.H., Khan, M.A., Mahmood, T., Ahmad, M., Chaudhary, M.F. and Khan, M.A., 2010. Shoot regeneration and free-radical scavenging activity in Silybum marianum. Plant Cell, Tissue and Organ Culture, 101: 371-376. - Ahmadi, E., Hosseini Nasr, S.M., Jalilvand, H. and Salehian Aghblaq, H., 2012. In vitro somatic propagation of Ziziphus spina christti (L.) Willd via indirect regeneration from leaf explants. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 20(1): 111-123. - Arekhi, S., Aghdasi, M. and Khalafi, M., 2012. Optimization of Silybum marianum tissue culture for production of medicinal flavonolignan. Journal of Plant Production, 19(2): 69-87. - Agarwal, S. and Kanwar, K., 2007. Comparison of genetic transformation in Morus alba L. via different regeneration systems. Plant Cell Report, 26: 177-185. - Bekheet, S.A., El-Bahr, M.K., Ali, S.A. and Hamed, M.A., 2014. Callus production of Globe artichoke and Milk thistle: In vitro hypolipidemic and antioxidant activities. World Journal of Pharmaceutical Research, 3(4): 1-17. - Cimino, C., Cavalli, S.V., Spina, F., Natalucci, C. and Priolo, N., 2006. Callus Culture for biomass production of milk thistle as a potential source of milk clotting peptidases. Electronic Journal of Biotechnology, 9(3): 237-240. - Dalal, N.V. and Rai, V.R., 2004. In vitro propagation of Oroxylum indicum Vent. a medicinally important forest tree. Journal for Research, 9: 61-65. - Dadvar, F., Rostami, T., Assare, M.H., Emam, M. and Shirvany, A., 2013. Effects of different concentrations of plant regulators on In vitro micropagation of Celtis caucasica. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 21: 13-23. - Thomas, T.D., 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advances, 26: 618-631. - Hetz, E., Perales, E.H., Liersch, R. and Schieder, O., 1995. Plant regeneration from mesophyll and suspension protoplasts of Silybum marianum. Planta Medica, 61(6): 554-557. - Iqbal Sada, M. and Srivastava, P., 2002. In vitro micropropagation of silybum marianum L. from various explants and silybin content. Plant Biochemistry and Biotechnology, 9(2): 81-87. - Iri, S., Aghdasi, M. and Mianabadi, M., 2013. Induction of root formation to produce silymarin in Silybium marianum plant in tissue culture condition. Journal of Plant Process and Function, 2(2): 1-12. - Joshi, P. and Dhawan, V., 2007. Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay. Biologia Plantarum, 51: 22-26. - Khalafalla, M.M., Elaleem, K.G. and Modawi, R.S., 2010. Callus formation and organogenesis of potato (Solanum tuberosum L.) cultivar almera. Journal of Phytology, 2: 40-46. - Kumar, N. and Reddy, M.P., 2011. In vitro plant propagation. Journal of Forest Science, 2: 61-72. - Maliti, C.M., Basile, D.V. and Corpe, W.A., 2005. Effects of Methylobacterium spp. Strains on rice Oryza sativa L. callus induction, plantlet regeneration, and seeding’s growth. In vitro Journal Torrey Botany Society, 132: 355-367. - Meiners, J., Schwab, M. and Szankowski, I., 2007. Efficient in vitro regeneration systems for Vaccinium species. Plant Cell Tissue Organ Culture, 89: 169-176. - Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. - Mulwa, R.M.S. and Bhalla, P.L., 2006. In vitro plant regeneration from immature cotyledon explants of macadamia (Macadamia tetraphylla L. Johnson). Plant Cell Report, 25: 1281-1286. - Nagori, R. and Purohit, S.D., 2004. In vitro plantlet regeneration in Annona squamosa through direct shoot bud differentiation on hypocotyl segments. Scientia Horticulturae, 99: 89-98. - Pierik, R.L.M., 1987. In vitro culture of higher plants. MortinusNijhof Publisher, Dordrecht, 348p. - Preece, J.E., 1995. Can nutrient salts partially substitute for plant growth regulators? Plant Tissue Culture Biotechnology, 1: 26-37. - Safarnejad, A., 2015. Effects of growth regulators on in vitro regeneration of Ziziphus jujube. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 23(1): 40-48. - Samandari Gikloo, T., Elhami, B. and Khosrowchahli1, M., 2012. Effects of explant type, plant growth regulators and activated charcoal on direct organogenesis of Silybum marianum. African Journal of Biotechnology, 11(37): 9023-9027. - Schenk, R.U. and Hilderandt, A.C., 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany, 50: 199-204. - Sharma, T., Modgil, M., Thakur, M., 2007. Factors affecting induction and development of in vitro rooting in apple rootstocks. Indian Journal of Experimental Biology, 45: 824-829. - Subramaniam, S., Vaughn, K., Carrier, D.J. and Clausen, E.C., 2008. Pretreatment of milk thistle seed to increase the silymarin yield: an alternative to petroleum ether defatting. Bioresour Technology, 99(7): 2501-2506. - Verma, S.K., Yucesan, B.B., Shahin, G., Gurel, S. and Gurel, E., 2011. Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. Turkish Journal of Botany, 35: 689-695. - Zarei, B., Taghipour, Z. and Kahrizi, D., 2020. Effect of growth regulators on in vitro callogenesis and regeneration of Atropa Belladonna. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 27(2): 231-239. | ||
|
Statistics Article View: 1,512 PDF Download: 558 |
||
Journal management system. designed by sinaweb