| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,873 |
| تعداد مقالات | 32,558 |
| تعداد مشاهده مقاله | 42,482,863 |
| تعداد دریافت فایل اصل مقاله | 19,247,846 |
اثر پیش‌تیمارهای دمایی و نوع کربوهیدرات بر القاء آندروژنز در کشت بساک Capparis spinosa L. | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 2، دوره 37، شماره 3 - شماره پیاپی 107، مرداد و شهریور 1400، صفحه 381-402 اصل مقاله (1.6 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2021.342563.2765 | ||
| نویسندگان | ||
| سیده معصومه مصطفوی1؛ محمدرضا عبداللهی* 2؛ دارا دستان3؛ حسن ساریخانی4 | ||
| 1دانش‌آموخته کارشناسی ارشد، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران | ||
| 2دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران | ||
| 3استادیار، گروه داروسازی، دانشگاه علوم پزشکی همدان، همدان، ایران | ||
| 4دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران | ||
| چکیده | ||
| گیاه کبر (Capparis spinosa L.)، یک منبع غنی از فلاونوئید روتین است که نقش مهمی در سلامتی انسان دارد. در تحقیق حاضر اثرات تیمارهای سرمایی (°C25 به عنوان شاهد، °C4 و °C7 به‌مدت‌های 2، 4 و 7 روز)، گرمایی (°C25 به‌عنوان شاهد، °C30 به‌مدت 14 روز، °C32 به‌مدت 2 و 4 روز و °C35 به‌مدت 8 ساعت) و کربوهیدرات بر کارایی آندروژنز در کشت بساک کبر بررسی گردید. همچنین اثرات مالتوز و ساکارز در غلظت‌های g L-1 30 و g L-1 60 در ترکیب با دو تیمار دمایی (1- °C30 به‌مدت 14 روز و 2- °C7 به‌مدت 7 روز + پیش‌تیمارهای آزاسیتیدین و 2,4-D) روی القاء آندروژنز بررسی شد. تیمارهای دمایی و کربوهیدرات اختلاف آماری معنی‌داری (0.01p ≤) از نظر تشکیل کالوس و رویان داشتند. تیمار °C7 به‌مدت 2، 4 و 7 روز بیشترین درصد (در هفته سوم: به‌ترتیب 80، 78.34 و 76.67 درصد) و سرعت کالوس‌زایی (به‌ترتیب 7.85، 7.75 و 7.60 کالوس در هفته) و تیمار °C7 به‌مدت 7 روز بیشترین تولید رویان (0.57 رویان در هر بساک) را ایجاد کردند. تیمار °C30 به‌مدت 14 روز بیشترین درصد (در هفته سوم: 100%) و سرعت کالوس‌زایی (9.44 کالوس در هفته) را داشت. در حالی‌که تیمارهای °C32 به‌مدت 2 و3 روز و همچنین °C30 به‌مدت 14 روز بیشترین تعداد رویان به‌ازای هر بساک (به‌ترتیب 0.22، 0.20 و 0.18 رویان) را تولید کردند. استفاده از g L-1 30 مالتوز در ترکیب با تیمار °C30 به‌مدت14 روز بیشترین درصد (در هفته سوم: 91.66%) و سرعت کالوس‌زایی (8.94 کالوس در هفته) را ایجاد کرد در حالی‌که g L-1 30 مالتوز در ترکیب با تیمار °C7 به‌مدت 7 روز + پیش‌تیمارهای آزاسیتیدین و 2,4-D، بیشترین میانگین تعداد رویان به‌ازای هر بساک (0.55 رویان) را تولید کرد. نتایج این تحقیقات جهت استفاده در برنامه‌های اصلاحی کبر از اهمیت بالایی برخوردار می باشد. | ||
| کلیدواژه‌ها | ||
| کبر (Capparis spinosa L.)؛ کشت بساک؛ کالوس‌زایی؛ رویان‌زایی؛ تیمار دمایی؛ کربوهیدرات | ||
| عنوان مقاله [English] | ||
| Effects of temperature pretreatments and carbohydrate type on androgenesis induction in anther culture of Capparis spinosa L. | ||
| نویسندگان [English] | ||
| S.M. Mostafavi1؛ M.R. Abdollahi2؛ D. Dastan3؛ H. Sarikhani4 | ||
| 1M.Sc. graduate, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran | ||
| 2Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran | ||
| 3Department of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 4Department of Horticultural sciences, Faculty of Agriculture, Bu-Ali University, Hamedan, Iran | ||
| چکیده [English] | ||
| Caper (Capparis spinosa L.) is a rich source of rutin, plays an essential role in human health. In the present study, the effects of cold (25°C as control, 4°C, and 7°C for 2, 4, and 7 days), heat (25°C as control, 30°C for 14 days, 32°C for 2 and 4 days, and 35°C for 8 hours), and carbohydrate treatments on the androgenesis efficiency were studied in the anther culture of caper. Also, the effects of maltose and sucrose at the concentrations of 30 and 60 g L-1 in combination with two temperature treatments (1- 30°C for 14 days and 2- 7°C for 7 days + azacytidine and 2,4-D pretreatments) on the androgenesis induction was evaluated. The temperature and carbohydrate treatments showed statistically significant differences (p < /em>≤0.01) in terms of callus and embryo formation. The 7°C for 2, 4, and 7 days produced the highest percentage (at the third week: 80, 78.34, and 76.67%, respectively) and callogenesis speed (7.85, 7.75, and 7.60 calli week-1, respectively) and the 7°C for 7 days produced the highest embryo production (0.57 embryo anther-1). The 30°C for 14 days treatment showed the highest percentage (at the third week: 100%) and callogenesis speed (9.44 calli week-1). While the 32°C for 2 and 3 days and also 30°C for 14 days produced the highest number of embryos per anther (0.22, 0.20, and 0.18 embryo, respectively). The use of 30 g L-1 maltose in combination with the 30°C for 14 days produced the highest percentage (at the third week: 91.66%) and callogenesis speed (8.94 calli week-1), while the 30 g L-1 maltose in combination with the 7°C for 7 days + azacytidine and 2,4-D pretreatments produced the highest mean embryo number per anther (0.55 embryo). The results of this research are of great importance for the use in the caper breeding programs. | ||
| کلیدواژه‌ها [English] | ||
| Capparis spinosa L, anther culture, callogenesis, embryogenesis, temperature treatment, carbohydrate | ||
| مراجع | ||
|
- Abdollahi, M.R., Moieni, A. and Jalali Javaran, M., 2004. Interactive effects of heat shock and culture density on embryo induction in isolated microspores culture of Brassica napus L. cv Global. Iranian Journal of Biotechnology, 2(2): 97-100. - Bajaj, Y.P.S., 1990. In vitro production of haploids and their use in cell genetics and plant breeding: 3-44. In: Bajaj, Y.P.S., (Ed.). Biotechnology in Agriculture and Forestry (Vol: 12): Haploids in Crop Improvement I. Springer, Berlin, 549p. - Burrell, A.M., Lineberger, R.D., Rathore, K.S. and Byrne, D.H., 2006. Genetic variation in somatic embryogenesis of Rose. HortScience, 41: 1165-1168. - Bohanec, B., Neskovic, M. and Vujicic, R., 1993. Anther culture and androgenetic plant regeneration in buckwheat (Fagopyrum esculentum Moench). Plant Cell Tissue and Organ Culture, 35: 259-266. - Chardoli Eshaghi, Z., Abdollahi, M.R., Moosavi, S.S., Deljou, A. and Segui-Simarro, J.M., 2015. Induction of androgenesis and production of haploid embryos in anther cultures of borage (Borago officinalis L.). Plant Cell Tissue and Organ Culture, 122: 321-329. - Cornelini, P., Federico, C. and Pirrera, G., 2008. Arbusti autoctoni mediterranei per l’ingegneria naturalistica. Primo contributo alla morfometria degli apparati radicali. Collana Sicilia Foreste, 331p. - Duncan, E.J. and Heberle, E., 1976. Effect of temperature shock on muclear phenomena in microspores of Nicotina tabacum and consequently on plantlet production. Protophasma, 90: 173-177. - Ferrie, A.M.R. and Caswell, K.L., 2011. Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue and Organ Culture, 104: 301-309. - Ferrie, A.M.R., Bethune, T. and Mykytyshyn, M., 2011. Microspore embryogenesis in the Apiaceae. Plant Cell Tissue and Organ Culture, 104: 399-409. - Ferrie, A.M.R., Palmer, C.E. and Keller, W.A., 1995. Haploid embryogenesis: 309-344. In: Thorpe, T.A., (Ed.). In Vitro Embryogenesis in Plants. Kluwer Academic Publishers, Dordrecht, The Netherlands, 558p. - Fletcher, R., Coventry, J. and Kott, L.S., 1998. Double Haploid Technology for Spring and Winter Brassica napus, Technical Bulletin, OAC Publication, Canada, 51p. - Gamborg, O.L., Miller, R.A. and Qjiwa, K., 1968. Nutrient requirements of suspension culture of soybean root callus. Experimental Cell Research, 50: 151-158. - Germana, M.A., 2011. Anther culture for haploid and doubled haploid production. Plant Cell, Tissue and Organ Culture, 104: 283-300. - George, E.F., Hall, M.A. and Klerk, G.J.D., 2008. Plant Propagation by Tissue Culture. Dordrecht, The Netherlands, Springer, 504p. - Gram, T., Mattsson, O. and Joersbo, M., 1996. Division frequency of pea protoplasts in relation to starch accumulation. Plant Cell, Tissue and Organ Culture, 45: 179-183. - Song, H., Lou, Q.F., Luo, X.D., Wolukau, J., Diao, W., Qian, C. and Chen, J.F., 2007. Regeneration of doubled haploid plants by androgenesis of cucumber (Cucumis sativus L.). Plant Cell Tissue and Organ Culture, 90: 245-254. - Ismaili, A. and Mohammadi, P.P., 2016. Effect of genotype induction medium carbohydrate source and polyethylene glycol on em-bryogenesis in maize (Zea mays L.) anther culture. Acta Physiologiae Plantarum, 38: 74. - Kim, C.K., Oh, J.Y., Chung, J.D., Burrel, A.M. and Byrne, D.H., 2004. Somatic embryogenesis and plant regeneration from in-vitro-grown leaf explant of rose. HortScience, 39: 1378-1380. - Maguire, J.D., 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177. - Majd, A., Chamandosti, F., Mehrabia, S. and Sheidai, M., 2006. Somatic embryogenesis and plant regeneration in Brassica napus L. Biological Science, 9: 729-734. - Nabavi, S.F., Maggi, F., Daglia, M., Habtemariam, S., Rastrelli, L. and Nabavi, S.M., 2016. Pharmacological Effects of Capparis spinosa L. Phytotherapy Research , 30: 1733-1744. - Nomizu, T., Niimi, Y. and Han, D., 2004. Haploid plant regeneration via embryogenesis from anther cultures of Hepatica nobilis. Plant Cell Tissue and Organ Culture, 79: 307-313. - Orsinky, B.L., McGregor, G.I., Johnson, G.I. and Kartha, K.K., 1990. Improved embryoid induction and green shoot regeneration from wheat anther cultures with medium with maltose. Plant Cell Reports 9: 365-369. - Patricia, M.A., Julio, R.D. ana Ana, I.H., 2012. IAPT/IOPB chromosome data 13. Taxon, 61(4): 889-902. - Rahnavard, R. and Razavi, N., 2016. A review on the medical effects of Capparis spinosa L. Advanced Herbal Medicine, 2: 44-53. - Santini, A. and Novellino, E., 2014. Nutraceuticals: beyond the diet before the drugs. Current Bioactive Compounds, 10: 1-12. - Shariatpanahi, M.E., Bal, U., Heberle-Bors, E. and Touraev, A., 2006a. Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiologia Plantarum, 127: 519-534. - Shariatpanahi, M.E., Belogradova, K., Hessamvaziri, L., Heberle-Bors, E. and Touraev, A., 2006b. Efficient embryogenesis and regeneration in freshly isolated and cultured wheat (Triticum aestivum L.) microspores without stress pretreatment. Plant Cell Reports, 25: 1294-1299. - Shariatpanahi, M.E., Shakib, A.M. and Meybodi, D.E., 2012. Haploid and its Applications in Genetics and Plant Breeding. ABRII Publication, Iran, 276p. - Sher, H. and Alyemeni, M.N., 2010. Ethnobotanical and pharmaceutical evaluation of Capparis spinosa L. validity of local folk and Unani system of medicine. Journal of Medicinal Plants Research, 4: 1751-1756. - Sharma, A.K. and Sharma, A., 1972. Chromosome Techniques. Butter Worths, University Park Press, London, Baltimore, 724p. - Smykalova, I., Smirous Jr, P., Kuboslova, M., Gasmanova, N. and Griga, M., 2009. Doubled haploid production via anther culture in annual, winter type of caraway (Carum carvi L.). Acta Physiologia Plantarum, 31: 21-31. - Sunderland, N. and Huang, B., 1987. Ultrastructural aspects of pollen dimorphism. International Review of Cytology, 107: 175-220. - Tang, Y., Xiaomei, L., Juan, L., Chao, M., Jia, L. and Huanxiu, L., 2012. Effect of different pretreatment on callus formation from anther in balsam pear (Momordica charantia L.). Journal of Medicinal Plants Research, 6: 3393-3395. - Trejo-Tapia, G., Amaya, U.M., Morales, G.S., Sa´nchez, A.D.J., Bonfil, B.M., Rodrı´guez-Monroy, M. and Jimenez-Aparicio, A., 2002. The effects of cold-pretreatment, auxins and carbon source on anther culture of rice. Plant Cell Tissue and Organ Culture, 71: 41-46. - Vahid, H., Rakhshandeh, H. and Ghorbani, A., 2017. Antidiabetic properties of Capparis spinosa L. and its components. Biomedicine & Pharmacotherapy, 92: 293-302. - Wojnarowiez, G., Caredda, S., Devaux, P., Sangwan, R. and Cle´ment, C., 2004. Barley anther culture: assessment of carbohydrate effects on embryo yield, green plant production and differential plastid development in relation with albinism. Journal of Plant Physiology, 161: 747-755. - Yadollahi, A., Abdollahi, M.R., Moieni, A. and Danaee, M., 2011. Effects of carbon source, polyethylene glycol and abscisic acid on secondary embryo induction and maturation in rapeseed (Brassica napus L.) microspore-derived embryos. Acta Physiologia Plantarum, 33: 1905-1912. - Yang, C., Zhao, Y., Wei, J., Zhao, L., Sui, C., Zhang, Z. and Cui, L., 2011. Factores affecting embryogenic callus production and plant regeneration in anther culture of Bupleurum chinense. Chinese Herbal Medicines, 3: 214-220. | ||
|
آمار تعداد مشاهده مقاله: 1,169 تعداد دریافت فایل اصل مقاله: 418 |
||