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بررسی بیان ژنهای HSPA2 و HSPB1 و هستیشناسی ژن در کبد مرغان بومی خراسان تحت تنش گرمایی حاد | ||
| علوم دامی | ||
| دوره 34، شماره 130، خرداد 1400، صفحه 53-62 اصل مقاله (1.77 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2020.126865.1948 | ||
| نویسندگان | ||
| رضا توحیدی* 1؛ محمدرضا نصیری2؛ علی جوادمنش3 | ||
| 1استادیار- مجتمع آموزش عالی تربت جام – دانشکده کشاورزی- گروه علوم دامی. | ||
| 2استاد- دانشگاه فردوسی مشهد- دانشکده کشاورزی- گروه علوم دامی. | ||
| 3استادیار- دانشگاه فردوسی مشهد- دانشکده کشاورزی- گروه علوم دامی. | ||
| چکیده | ||
| دمای بالا یکی از فاکتورهای اصلی محیطی مؤثر بر کاهش سود اقتصادی واحدهای پرورش طیور است، زیرا رشد و تولید پرنده را مختل میکند. پروتئینهای شوک گرمایی (HSPs) نقش کلیدی در مکانیسم دفاعی سلولی علیه گرمای محیط دارند. هدف از انجام مطالعه حاضر، بررسی میزان بیان ژنهای HSPA2 و HSPB1 در کبد مرغان بومی خراسان تحت تنش گرمایی حاد بود. برای این هدف، تعداد 16 قطعه جوجه در سن 42 روزگی به دو گروه شاهد (دمای C 25 و رطوبت %50) و تیماری (دمای 40 و C 42 و رطوبت %50 به مدت 24 ساعت) تقسیم شده و پس از کشتار از کبد آنها نمونهبرداری به عمل آمد. سپس میزان بیان ژنهای HSPA2 و HSPB1 با روش RT-qPCR مورد ارزیابی قرار گرفت. بیان ژن HSPA2 نسبت به گروه شاهد افزایش معنیداری داشت (05/0 P)، در حالی که برای ژن HSPB1 افزایش معنیداری مشاهده نشد. آنالیز شبکه پروتئینی وجود برهمکنش پروتئینی بین HSPA2 و HSPB1 نشان نداد ولی بر اساس آماره FDR در هستیشناسی ژن چهار مسیر فرایند پروتئینی در شبکه آندوپلاسمی، بلوغ اووسیت وابسته به پروژسترون، و مسیرهای علامت دهی MAPK و VEGF بیشترین معنیداری را داشتند. عدم وجود تلفات و همچنین نتایج آنالیز بیان ژن نشان میدهند که مرغان بومی خراسان توانایی مقاومت در برابر تنش گرمایی را دارند و نسبت به تنش گرمایی حاد واکنش ژنی نشان میدهند، و از طرفی ژن HSPA2 توانایی بیان در این شرایط محیطی را به جهت حفظ ساختار پروتئینی درون سلولی دارد. | ||
| کلیدواژه‌ها | ||
| تنش گرمایی؛ پروتئین شوک گرمایی؛ بیان ژن؛ مرغ بومی خراسان | ||
| عنوان مقاله [English] | ||
| A study on the expression of HASPA2 and HSPB1 genes and gene ontology in the liver of Khorasan native chickens under acute heat stress | ||
| نویسندگان [English] | ||
| Reza Tohidi1؛ Mohammadreza Nassiri2؛ Ali Javadmanesh3 | ||
| 1Dept. of Animal Science, Faculty of Agriculture, The University of Torbate-jam | ||
| 2Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 3Department of Animal Science-Faculty of Agriculture-Ferdowsi University of Mashhad-Mashhad-Iran | ||
| چکیده [English] | ||
| Heat stress is one of the main environmental factors causing economic losses to the poultry industry, as it reduces growth and production performance of chickens. The heat shock proteins (HSPs) involved in the key cellular defense mechanisms during exposure in hot environmental conditions. Elevated level of expression of HSPs helps to overcome the stress caused by high temperatures. The aim of this study was to evaluate the expression level of HSPA2 and HSPB1 transcripts in the liver of Khorasan native chickens under acute heat stress. Sixteen 42days old chickens were divided into two groups of control (25 C and 50% humidity) and heat treated (40 and 42 C, 50% humidity for 24h), and then the liver was sampled after killing. The transcription level of HSPA2 and HSPB1 was evaluated using RT-qPCR method. The HSPA2 expression was significantly induced (P0.05). However, the expression of HSPB1 did not show any change. The analysis of protein-protein interaction revealed that HSPA2 and HSPB1 had no interaction, however, four pathways in gene ontology including Protein processing in endoplasmic reticulum, Progesterone-mediated oocyte maturation, MAPK signaling pathway and VEGF signaling pathway had the highest significancy. The results indicated that Khorasan native chickens have suitable genetic reaction to acute heat stress and HSPA2 has the ability to express under high ambient temperature to protect the structure of cellular proteins. | ||
| کلیدواژه‌ها [English] | ||
| heat stress, heat shock protein, gene expression, Khorasan native chicken | ||
| مراجع | ||
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