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[مقاله کوتاه علمی] اثر ضد باکتریایی عصاره اتانولی گیاهان آویشن و زیره سیاه بر عوامل موثر بر بیماری‌زایی باکتری Pectobacterium carotovorum subsp. carotovorum | ||
| مهار زیستی در گیاه‌پزشکی | ||
| دوره 8، شماره 1 - شماره پیاپی 15، شهریور 1399، صفحه 165-175 اصل مقاله (266.56 K) | ||
| نوع مقاله: مقاله کوتاه | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2020.124239 | ||
| نویسندگان | ||
| فرانک رحیمی؛ ساغر کتابچی* | ||
| گروه بیماری‌شناسی گیاهی و میکروبیولوژی، دانشکده علوم، کشاورزی و تکنولوژی‌های نوین، دانشگاه آزاد اسلامی واحد شیراز، شیراز، ایران | ||
| چکیده | ||
| باکتریPectobacterium carotovorumعامل بیماری پوسیدگی نرم سیب‌زمینی است. این تحقیق به‌منظور یافتن راهکار مؤثر و بی‌خطر جهت کاهش خسارت این بیماری انجام شده است.در این پژوهش اثر ضد باکتریایی عصاره اتانولی دو گیاه دارویی آویشن شیرازی و زیره سیاه برروی عوامل موثر بر بیماری‌زایی بیماری پوسیدگی نرم سیب‌زمینی در قالب طرح کاملاً تصادفی مورد بررسی قرار گرفت و فاکتورهای قطر هاله، درصد بازدارندگی، میزان بیوفیلم، تأثیر عصارههای گیاهی بر درصد آبگریزی، میزان چسبندگی و حرکت باکتری، اندازه‌گیری و تجزیه و تحلیل شد.نتایج آزمایش نشان داد بیشترین میزان قطر هاله چاهک در اثر کاربرد عصاره گیاهان آویشن برابر با 66/9 میلیمتر می‌باشد. همچنین عصاره آویشن تأثیر قابل ملاحظهای بر درصد بازدارندگیPectobacterium carotovorum در غده سیب‌زمینی به میزان 46/87 درصد دارد و باعث کاهش علائم بیماری روی غده سیب‌زمینی شد. اثرات گیاه آویشن و زیره سیاه بر روی دیگر فاکتورهای بیماری‌زایی به یک اندازه بوده است و هر دو گیاه از نظر آماری با شاهد تفاوت معنی‌داری را نشان دادند. عصاره آویشن باداشتن خاصیت ضد باکتریایی بیشتر و مهار حرکت باکتری باعث کنترل معنی‌دار بیماری روی غده سیب‌زمینی شده است. با توجه به نتایج این تحقیق استفاده از ترکیبات موثر عصاره آویشن جهت کنترل بیماری پوسیدگی نرم غده سیب‌زمینی بویژه در دوران انبارداری توصیه می‌گردد. | ||
| کلیدواژه‌ها | ||
| Pectobacterium carotovorum؛ اثرضدمیکروبی؛ عصاره الکلی؛ فاکتور ویرولانس؛ پوسیدگی نرم سیب‌زمینی | ||
| عنوان مقاله [English] | ||
| Antibacterial effect of ethanolic extract of Zataria multiflora and Carum carvi on factors affecting pathogenicity of Pectobacterium carotovorum subsp. caratovorum | ||
| نویسندگان [English] | ||
| Faranak Rahimi؛ Saghar Ketabchi | ||
| Department of Plant Pathology, Collage of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran | ||
| چکیده [English] | ||
| Pectobacterium carotovorum is the causative agent of potato soft rot. The aim of this study was to find an effective and safe solution to reduce the damage of this disease. The antibacterial effect of ethanolic extract of two medicinal plants i.e. thyme and black cumin on the pathogenic factors of P. carotovorum was investigated in a completely randomized design and the factors of inhibition zone diameter, inhibition percentage, biofilm, hydrophobic effect, adhesion on the potato tuber and motility of bacteria were measured and analyzed. The results showed that the maximum diameter of the inhibition zone due to the use of thyme extract was 9.66 mm. Also, thyme plant extract had a significant effect on the inhibitory percentage of P. carotovorum in the potato tuber by 87.46% and reduced the symptoms of the disease on the potato tuber. Effects of thyme and black cumin extracts on the other pathogenic factors were the same and both extracts showed statistically significant differences with the control. It seems that thyme extract had more antibacterial properties and because of inhibition of motility of bacteria caused significant control of the disease on the potato tubers. According to the results of this study, the use of effective compounds of thyme extract to control soft rot disease of potato tuber, especially during storage is recommended. | ||
| کلیدواژه‌ها [English] | ||
| Pectobacterium cartovorum, antimicrobial, alcoholic extract, virulence factor, potato soft rot | ||
| مراجع | ||
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