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تأثیر واکشت بر تنوع سوماکلونال کالوس بنگدانه (Hyoscyamus niger) با استفاده از نشانگرهای ISSR | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| دوره 28، شماره 2 - شماره پیاپی 56، اسفند 1399، صفحه 203-211 اصل مقاله (278.21 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2021.352289.1374 | ||
| نویسندگان | ||
| سارا طهماسبی گوجگی1؛ امیر موسوی* 2؛ کمال الدین حق بین3؛ خسرو پیری4 | ||
| 1دانش‌آموخته دکتری، گروه علوم باغبانی و زراعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران | ||
| 2نویسنده مسئول مکاتبات، دانشیار، پژوهشگاه ملی ژنتیک و زیست‌فناوری، پژوهشکده بیوتکنولوژی گیاهی، تهران پست‌الکترونیک: m-amir@nigeb.ac.ir | ||
| 3استاد، پژوهشگاه ملی ژنتیک و زیست‌فناوری، پژوهشکده بیوتکنولوژی گیاهی، تهران | ||
| 4استاد، دانشگاه بوعلی سینا همدان، دانشکده ژنتیک و به‌نژادی گیاهی، همدان | ||
| چکیده | ||
| به‌منظور بررسی تنوع ژنتیکی در کشت درون شیشه‌ای دو نمونه کالوس گیاه دارویی بنگدانه (Hyoscyamus niger) شامل کالوس جدید (یک ماهه) و کالوس قدیمی (یکساله) در مقابل برگهای تازه گیاه، از نشانگر تکرار توالی ساده (ISSR) استفاده شد. با استفاده از ده آغازگر 49/6 درصد چندشکلی در سه نمونه ذکرشده مشاهده شد. تجزیه وتحلیل واریانس مولکولی (AMOVA) 68 درصد تنوع ژنتیکی را بین نمونه‌ها نشان داد. همچنین از ضرایب (ماتریس تشابه) جاکارد و دایس با روشهای UPGMA و NJ استفاده شد. بهترین دندروگرام برای نشان دادن تنوع ژنتیکی (دوری یا نزدیکی افراد)، استفاده از ضریب تشابه جاکارد بود. نتایج آنالیز خوشه‌ای نمونه‌ها را در دو گروه متفاوت گروه‌بندی کرد. این دسته‌بندی توسط تجزیه و تحلیل مؤلفه‌های اصلی (PCoA) نیز تأیید شد. گروه اول شامل کالوسهای قدیمی و گروه دوم شامل کالوسهای جدید و برگهای گیاه بود. نتایج حکایت از این داشتند که تنوع سوماکلونال در کالوسهای بنگدانه با افزایش تعداد دفعات واکشت افزایش می‌یابد. | ||
| کلیدواژه‌ها | ||
| آلکالوئیدهای پیرولیزیدین؛ ارزیابی ژنتیکی؛ تنوع سوماکلونال؛ Jaccard؛ PCoA؛ NJ | ||
| عنوان مقاله [English] | ||
| The effect of subculture on somaclonal variation in callus culture of Hyoscyamus niger using ISSR markers | ||
| نویسندگان [English] | ||
| S. Tahmasebi Goojgi1؛ A. Mousavi2؛ K. Haghbeen3؛ K. Piri4 | ||
| 1PhD Graduated, Department of Horticulture Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, I.R. Iran. | ||
| 2Corresponding author, Assoc. prof. National Institute of Genetic Engineering and Biotechnology, Department of Plant Biotechnology, Tehran, I.R. Iran. Email: m-amir@nigeb.ac.ir. | ||
| 3Prof. National Institute of Genetic Engineering and Biotechnology, Department of Plant Biotechnology, Tehran, I.R. Iran | ||
| 4Prof. Bu-Ali Sina University, Department of Genetics and Plant Breeding, Tehran, I.R. Iran | ||
| چکیده [English] | ||
| To evaluate the genetic variation in an in vitro culture of two samples of Hyoscyamus niger callus including new (one-month-old) and old (one-year-old) calli versus fresh leaves of the plant, the Inter Simple Sequence Repeat (ISSR) marker was used. Using 10 primers, 6.49% polymorphism was observed in the three mentioned samples. Analysis of Molecular Variance (AMOVA) revealed 68% genetic variation among samples. Jaccard and Dice coefficients (similarity matrix) were also used by UPGMA and NJ methods. The best dendrogram to show genetic diversity (and distance or proximity of individuals) was known as the Jaccard similarity coefficient. The results of cluster analysis grouped the samples into two different groups. This classification was also confirmed by principal component analysis (PCoA). The first major group consisted of old calli and the second involved new calli and plant leaves. The result showed that the frequency of somaclonal variation in H. niger calli tended to increase with increasing the number of subculture times. | ||
| کلیدواژه‌ها [English] | ||
| Pyrrolizidine alkaloids, Genetic assessment, Somaclonal variation, Jaccard, PCoA, NJ | ||
| مراجع | ||
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