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اثر عصاره آبی- الکلی Ducrosia anethifolia (Dc.) Boiss. بر بافت قلب جنین موش بزرگ آزمایشگاهی دیابتی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 5، دوره 37، شماره 1 - شماره پیاپی 105، فروردین و اردیبهشت 1400، صفحه 52-64 اصل مقاله (1.04 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2021.342950.2791 | ||
| نویسندگان | ||
| ملیحه کرمی1؛ فرنگیس قاسمی* 2 | ||
| 1دانش آموخته کارشناسی ارشد، گروه زیست‌شناسی، واحد جهرم، دانشگاه آزاد اسلامی، جهرم، ایران | ||
| 2استادیار، گروه زیست‌شناسی، واحد جهرم، دانشگاه آزاد اسلامی، جهرم، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1400.37.52.105.1.1575.1610 این پژوهش با هدف بررسی اثر عصاره آبی–الکلی برگ گیاه مشگک (Ducrosia anethifolia (DC.) Boiss.) بر تغییرات بافت قلب در جنین موش آزمایشگاهی دیابتی انجام شد. بدین منظور 48 سر موش ماده 200 تا 220 گرمی با هشت موش نر 230 تا 250 گرمی لقاح داده شدند. پس از اطمینان از بارداری، 32 سر ماده با تزریق داخل صفاقی 80 میلی‌گرم استرپتوزوتوسین به ازای هر کیلوگرم وزن بدن در اولین روز بارداری، دیابتی و به‌صورت تصادفی گروه‌بندی شدند. گروه‌ها شامل کنترل (موش‌های سالم بدون تیمار)، شاهد 1 (موش‌های سالم دریافت‌کننده یک میلی‌لیتر عصاره مشگک)، شاهد 2 (موش‌های دیابتی بدون تیمار)، و سه گروه تیمار که به‌ترتیب یک میلی‌لیتر از عصاره مشگک با دوزهای 250، 500 و 1000 کیلوگرم به ازای هر کیلوگرم وزن بدن موش دیابتی از روز 6 تا 20 دوره بارداری به‌طریق گاواژ دریافت کردند، بودند. روز بیستم بارداری پس از بیهوشی موش‌ها و گرفتن دو میلی‌لیتر خون از قلب آنها، جنین‌ها از رحمشان خارج شدند. قلب جنین‌ها جدا و در محلول 5% فرمالین تثبیت گردید. بعد از آماده‌سازی بافتی، مقاطع پنج میکرونی به روش استریولوژیکی تهیه و با هماتوکسیلین و ائوزین رنگ‌آمیزی شدند. محیط قلب و ضخامت دیواره آن با نرم‌افزار Image analysis و تعداد سلول‌های قلب با نرم‌افزار Grids-stero lite اندازه‌گیری گردید. داده‌ها با آزمون‌های آماری ANOVA تحلیل و میانگین‌ها با آزمون توکی مقایسه شدند (0.05≥p ). یافته‌ها حاکی از افزایش گلوکز خون، تراکم و طول سلول‌های قلبی و محیط قلب در همه گروه‌های دیابتی در مقایسه با کنترل بود که در موش‌های دیابتی تیمار شده با عصاره مشگک در مقایسه با گروه دیابتی بدون تیمار کاهش معنی‌دار یافتند. مطابق با نتایج حاصل، عصاره آبی-الکلی برگ مشگک با کاهش گلوکز خون در موش‌های دیابتی، توانست تغییرات تخریبی ناشی از دیابت در بافت قلب و عروق جنین را تا حدودی جبران کند. | ||
| کلیدواژه‌ها | ||
| قند خون؛ کاردیوسیت؛ عصاره مشگک (Ducrosia anethifolia (Dc.) Boiss.)؛ میکرومتری | ||
| عنوان مقاله [English] | ||
| Effects of aqueous-alcoholic extract of Ducrosia anethifolia (DC.) Boiss. leaves on fetal heart tissue in diabetic rats | ||
| نویسندگان [English] | ||
| M. Karami1؛ F. Ghassemi2 | ||
| 1M.Sc., Graduated, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran | ||
| 2Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran | ||
| چکیده [English] | ||
| This study aimed to investigate the effects of aqueous-alcoholic extract of Ducrosia anethifolia (DC.) Boiss. leaves on the fetal heart tissue changes in the diabetic rats. Forty-eight female rats weighing 200-220 g were fertilized with eight male rats weighing 230-250 g. After ensuring the pregnancy, the females became diabetic by intraperitoneal injection of streptozotocin (80 mg kg-1 body weight) on the first day of pregnancy, and were randomly grouped. Groups included Control (healthy rats without treatment), Sham 1 (healthy rats receiving 1 ml D. anethifolia extract), Sham 2 (diabetic rats without treatment), and three groups of treatments which respectively received 1 ml D. anethifolia extract at the doses of 250, 500, and 1000 kg kg-1 body weight of the diabetic rats from 6th to 20th day of the pregnancy by gavage. On the 20th day of pregnancy, after anesthetizing the rats and taking 2 ml of blood from their hearts, the fetuses were removed from their uterus. The fetal hearts were isolated and fixed in the 5% solution of formalin. After the tissue processing, the five-micron sections were prepared by the stereological method and stained with hematoxylin and eosin. The heart circumference and its wall thickness were measured by the image analysis software, and the number of heart cells was counted by the Grids-stero lite software. The data were analyzed by the ANOVA and the means were compared by the Tukey test (p < /em>≤0.05). The findings showed an increase in the blood glucose, density and length of cardiocytes, and heart circumference in all the diabetic groups compared to the control, which were significantly reduced in the diabetic rats treated with D. anethifolia extract compared to the diabetic group without treatment. According to the results, the aqueous-alcoholic extract of D. anethifolia leaves could slightly compensate the destructive changes caused by diabetes in the fetal cardiovascular tissue by reducing the blood glucose in the diabetic rats. | ||
| کلیدواژه‌ها [English] | ||
| Blood glucose, cardiocyte, Meshgak extract (Ducrosia anethifolia (Dc.) Boiss.), micrometry | ||
| مراجع | ||
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