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اثر متابولیت‌ها و آنتی‌اکسیدان‌ها بر تحمل به یخ‌زدگی ژنوتیپ‌های عدس در شرایط کنترل شده | ||
| نهال و بذر | ||
| دوره 36، شماره 3، آذر 1399، صفحه 273-299 اصل مقاله (1.24 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/sppi.2020.123483 | ||
| نویسندگان | ||
| جعفر نباتی* 1؛ احمد نظامی2؛ سیده محبوبه میرمیران3؛ سید سعید حجت1؛ محمد کافی4 | ||
| 1پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| 2دانشکده کشاورزی و پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| 3گروه کشاورزی دانشگاه پیام نور، تهران ایران. | ||
| 4گروه آگروتکنولوژی و پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| چکیده | ||
| این مطالعه جهت به‌گزینی تحمل به یخ‌زدگی (13-، 15- و 18- درجه‌سانتیگراد) 40 ژنوتیپ عدس به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی تحت شرایط کنترل‌شده در سال 1397 در دانشگاه فردوسی مشهد انجام شد. بین ژنوتیپ‌های موردمطالعه ازنظر درصد بقا بعد از تنش یخ‌زدگی و محتوای کلروفیل a، کاروتنوئیدها، آنتوسیانین، کربوهیدرات‌های محلول، پرولین، مالون‌دی‌آلدئید، فنل، پروتئین، DPPH، آسکوربات‌پراکسیداز و پراکسیداز قبل‌از تنش یخ‌زدگی تفاوت معنی‌داری وجود داشت. مدل رگرسیونی نشان داد که کلروفیل b بیشترین تأثیر مثبت و پروتئین و مالون‌دی‌آلدئید بیشترین تأثیر منفی را بر درصدبقا داشتند. تجزیه خوشه‌ای، ژنوتیپ‌ها را به پنج گروه تفکیک کرد. برای بیشتر صفات مورد مطالعه ژنوتیپ‌های گروه چهارم و پنجم نسبت به میانگین کل و ژنوتیپ‌های سایر گروه‌ها برتری داشتند. در گروه چهارم محتوای آنتوسیانین، پروتئین، مالون‌دی‌آلدئید، فنل، پرولین، آسکوربات‌پراکسیداز و پراکسیداز نسبت به میانگین کل و سایر گروه‌ها برتری داشت. درصدبقا، محتوای کلروفیلb، کاروتنوئیدها، کل رنگ‌دانه‌ها و DPPH گروه پنجم نسبت به سایر گروه‌ها برتر بودند. تجزیه به مؤلفه‌های اصلی نشان داد که ژنوتیپ‌های MLC469، MLC458، MLC409، MLC74، MLC84،MLC169 ، MLC394، MLC95، MLC17، MLC163 و MLC303 ازنظر ظرفیت آنتی‌اکسیدانی و متابولیت‌ها و ژنوتیپ‌های MLC70، MLC410، MLC47، MLC31، MLC91، MLC8، MLC286، MLC407 وMLC472 ، MLC61، MLC83 و MLC334 ازنظر ظرفیت رنگ‌دانه‌های فتوسنتزی از وضعیت مطلوبی برخوردار هستند. به‌طورکلی، به‌نظر می‌رسد این شاخص‌ها قبل از وقوع تنش سرما اهمیت زیادی در پیش‌بینی تأثیر سرما بر درصد بقا ژنوتیپ‌های عدس دارند. | ||
| کلیدواژه‌ها | ||
| عدس؛ درصد بقا؛ پرولین؛ رنگ‌دانه؛ آنتوسیانین | ||
| عنوان مقاله [English] | ||
| Effect of Metabolites and Antioxidants on Freezing Tolerance of Lentil Genotypes Under Controlled Conditions | ||
| نویسندگان [English] | ||
| J. Nabati1؛ A. Nezami2؛ S. M. Mirmiran3؛ S. S. Hojjat1؛ M. Kafi4 | ||
| 1Research Center for Plant Sciences, Ferdowsi University of Mashhad, Iran. | ||
| 2Faculty of Agriculture and Research Center for Plant Sciences, Ferdowsi University of Mashhad, Iran. | ||
| 3Department of Agronomy, Payam-e-Noor University, Tehran, Iran. | ||
| 4Agrotechnolgy Group and Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| چکیده [English] | ||
| To study freezing tolerance (-13, -15 and -18°C) of 40 lentil (Lens culinaris Medik.) genotypes, a factorial experiment in completely randomized design was carried out at the Research Center of Plant Science, Ferdowsi University of Mashhad, Mashhad, Iran, in 2017-2018. Significant differences observed between genotypes for survival percentage after freezing stress and chlorophyll a, carotenoids, anthocyanins, soluble carbohydrates, proline, malondialdehyde (MDA), phenol, protein, DPPH, ascorbate peroxidase and peroxidase before freezing stress. Stepwise regression analysis showed that chlorophyll b had the most positive effect and protein, and MDA had the most negative effect on survival percentage. Cluster analysis grouped genotypes into five clusters. For most of the studied traits, genotypes in clusters 4 and 5 were greater than average of all genotypes as well as genotypes in other clusters. In clusters 4, anthocyanins, protein, MDA, phenol, proline, ascorbate peroxidase and peroxidase contents were higher than other clusters. Survival percentage, chlorophyll b, carotenoids, total pigments, and DPPH of cluster 5 were higher than the other clusters. Principal component analysis showed that MLC469, MLC458, MLC409, MLC74, MLC84, MLC169, MLC394, MLC95, MLC17, MLC163 and MLC303 for antioxidant capacity and metabolites, and MLC70, MLC410, MLC47, MLC31, MLC91, MLC8, MLC286, MLC407, MLC472, MLC61, MLC83 and MLC334 for photosynthetic pigment capacity were more suitable than the other genotypes. It can be concluded that these attributes are very important in predicting, before cold stress, the effect of cold stress on survival percentage of lentil genotypes. | ||
| کلیدواژه‌ها [English] | ||
| Lentil, pigment, proline, regression model, survival percentage, anthocyanin | ||
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