اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,083
تعداد مقالات34,401
تعداد مشاهده مقاله48,357,175
تعداد دریافت فایل اصل مقاله79,046,222
Javaheri, Golrokh, Doudi, Monir, Rahimzadeh Torabi, Ladan, Pazandeh, Mohammad Hossein. (1401). Nicotiana plumbaginifolia Leaf Extracts as an Efficient Antibacterial and a Biocontrol Agent Versus Tomato Pathogenic Bacteria. سامانه مدیریت نشریات علمی, 11(Special), 87-93. doi: 10.22092/jmpb.2020.352012.1261
Golrokh Javaheri; Monir Doudi; Ladan Rahimzadeh Torabi; Mohammad Hossein Pazandeh. "Nicotiana plumbaginifolia Leaf Extracts as an Efficient Antibacterial and a Biocontrol Agent Versus Tomato Pathogenic Bacteria". سامانه مدیریت نشریات علمی, 11, Special, 1401, 87-93. doi: 10.22092/jmpb.2020.352012.1261
Javaheri, Golrokh, Doudi, Monir, Rahimzadeh Torabi, Ladan, Pazandeh, Mohammad Hossein. (1401). 'Nicotiana plumbaginifolia Leaf Extracts as an Efficient Antibacterial and a Biocontrol Agent Versus Tomato Pathogenic Bacteria', سامانه مدیریت نشریات علمی, 11(Special), pp. 87-93. doi: 10.22092/jmpb.2020.352012.1261
Javaheri, Golrokh, Doudi, Monir, Rahimzadeh Torabi, Ladan, Pazandeh, Mohammad Hossein. Nicotiana plumbaginifolia Leaf Extracts as an Efficient Antibacterial and a Biocontrol Agent Versus Tomato Pathogenic Bacteria. سامانه مدیریت نشریات علمی, 1401; 11(Special): 87-93. doi: 10.22092/jmpb.2020.352012.1261

Nicotiana plumbaginifolia Leaf Extracts as an Efficient Antibacterial and a Biocontrol Agent Versus Tomato Pathogenic Bacteria

Journal of Medicinal plants and By-products
دوره 11، Special، تیر 2022، صفحه 87-93    اصل مقاله (510.81 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2020.352012.1261
نویسندگان
Golrokh Javaheri1؛ Monir Doudi* 1؛ Ladan Rahimzadeh Torabi1؛ Mohammad Hossein Pazandeh2
1Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
چکیده
Antibacterial agents derived from plants are known as suitable alternatives for synthetic pesticides because of their health and the lack of any side effects on plants. This study aims to assess in vitro effect of water, ethanol and methanol extracts of tobacco (Nicotiana plumbaginifolia Viv.) leaves on plant pathogenic bacteria and their effect on the control of bacterial infection in tomato plant in the farm conditions. Also, effective compounds of the selected extract were characterized. Water, ethanol and methanol extracts were prepared from the leaves of N. plumbaginifolia by soaking method. Antibacterial effects of three extracts were determined by agar well diffusion method in 100, 400 and 800 mg/ml concentrations on plant pathogenic bacteria including Xantomonas campestris PTCC1473, Pseudomonas aeruginosa PTCC1181, Pseudomonas syringae PTCC1290, Enterobacter aerogenes PTCC1221, Clavibacter michiganensis PTCC1399, Ralstonia solanacearum PTCC1600 and Gluconacetobacter PTCC1734. Then the effect of the extract was analyzed on the protection of infection and some characteristics of the tomato fruit in farm conditions. For this purpose, the tomato bushes were inoculated with the bacterium that were highly sensitive to the extracts and in order to evaluate the effect of the extract on the tomato fruit characteristics, factors including pH, ascorbic acid content, lycopene content, texture stiffness and antioxidant activity of tomatoes were assessed post treatment. Effective compounds of the extract with the maximum antibacterial effect were measured using GC/MS method. Water, ethanol and methanol extracts of N. plumbaginifolia in the minimum concentration (MIC) of 400 mg/ml were effective on the most of studied bacteria and had the most effect on P. aeruginosa PTCC1181 with the growth inhibition zone of 22.51 mm (water extract), 19.39 mm (ethanol extract) and 20.94 mm (methanol extract). The concentration of 800 mg/ml of the extracts was detected as minimum bactericidal concentration (MBC). The results showed that the methanol extract of N. plumbaginifolia had the most effect on the studied bacteria without changes in plant growth indicators. GC/MS analysis of the extract approved the existence of several organic acids and antimicrobial compounds including benzoic acid, hydroxymethylfurfural, nicotine, nonanoic acid, neophytadiene and plant esters. The methanol extract of N. plumbaginifolia leaves had the greatest impact on P. aeruginosa PTCC1181 as one of the pathogens of tomato, and this extract also controlled the growth of bacteria on the tomato plant in farm condition and this plant extract is recommended as an antimicrobial agent to control the bacterial disease of tomato.
کلیدواژه‌ها
Nicotiana plumbaginifolia؛ Antibacterial؛ Biocontrol agent؛ Plant pathogenic bacteria؛ Effective compounds
مراجع
  1. Knapp S, Chase M.W. Clarkson J.J. Nomenclatural changes and a new sectional classification in Nicotiana (Solanaceae). Taxon. 2004;53:73-82.
  2. Chase MW, Knapp S, Cox AV, Clarkson J, Butsko Y, Joseph J, Savolainen V, Parokonny V. Molecular systematics, GISH and the origin of hybrid taxa in Nicotiana (Solanaceae). Annals of Botany. 2003;92:107-127.
  3. Kaczorowski RL., Gardener MC., Holtsford TP. Nectar traits in Nicotiana section Alatae (Solanaceae) in relation to floral traits, pollinators, and mating system. American J Botany. 2005;92:1270-1283.
  4. Aunurohim NDA, Kuswytasari ND. Potency of Nicotiana tabacum as Anti – Microfouling. Published by the American Institute of Physics. 0.1063/1.4985396. 2016.
  5. Chunping XU, Shanshan Z, Mengshan L, Ya D, Lanlan T, Yanqi L. Chemical composition, antimicrobial and antioxidant activities of essential oil from fluecured tobacco flower bud. Biotech Biotechnological Equipment. 2016;30:1026-1030.
  6. Patil RS, Desai AB, Wagh SA. Comparative study of antimicrobial compounds extracted from leaves of Nicotiana tabacum and cigarette. World J Pharmacy Pharmaceutical Sci. 2015;4:1511-8.
  7. Davis DL, Nielson MT. Tobacco: production, chemistry, and technology. Blackwell Sci. 1999, pp. 260-284.
  8. Harlan WR., Moseley JM. Moseley, Tobacco. In Othmer, D. (ed), Kirk-Othmer encyclopedia of chemical technology. John Wiley & Sons. New York, USA, 1955, pp. 242-61.
  9. Agokei OE., Adebisi AA. Tobacco as an anesthetic for fish handling procedures. J Medicinal Plants Research. 2010;4:1396-1399.
  10. Shams S, Pourseyedi SH. Green synthesis of silver nanoparticles in Melia azedarach fruit extract and screening its antimicrobial activity. J Plant Productions Agronomy, Breeding and Horticulture. 2015;38:55-63.
  11. Kishioka S, Kiguchi N, Kobayashi Y, Saika F. Nicotine effects and the endogenous opioid system. J Pharmacological Sci. 2014;125:117-124.
  12. Adebayo-Tayo BC, Odu NN, Esen CU, Okonko IO. “Microorganisms associated with spoilage of stored vegetables in Uyo metropolis, AkwaIbom state, Nigeria,” Nature Sci. 2012;10.
  13. Yusuf AJ, Abdullahi MI., Aleku GA., Ibrahim IA, Alebiosu CO, Yahaya M, Adamu HW, Sanusi A, Mailafiya MM, Abubakar H. Antimicrobial activity of stigmasterol from the stem bark of Neocarya macrophylla. J Med Plants Eco Development. 2018;2:1-5.
  1. Singh KP, Daboriya V, Kumar S, Singh S. Antibacterial activity and phytochemical investigations on Nicotiana plumbaginifolia viv. (wild tobacco). Romanian J Biology-Plant Biology. 2010;55:135-142.
  2. Okorondu SI, Okorondu MMO, Oranusi SC. Antimicrobial effect of Nicotiana tabacum (Tobacco) leaf extract on Staphylococcus aureus and Escherichia coli. Nigerian J Microbiology. 2015;29:3049-3061.
  3. Wilson HO, Gisvold O, Block JH. Wilson and Gisvold's textbook of organic medicinal and pharmaceutica . Lippincott Williams and Wilkins. Philadelphia. 2004.
  4. Lillard B. Practical druggist and pharmaceutical review of reviews. Lillard Clark Company. USA. 1904.
  5. Abraham K, Gürtler R, Berg K, Heinemeyer G, Lampen A, Appel K.E. Toxicology and risk assessment of 5-Hydroxymethylfurfural in food. Molecular Nutr and Food Research. 2011;55:667-678.
  6. Nafea EA, Moselhy WA, Fawzy A. Does the HMF value affect the antibacterial activity of the bee honey. Egyptian Academic J Biological Sci. 2011;4:13-19.
  7. Laird T. Ullmann's encyclopedia of industrial chemistry. 7th ed., Weinheim: VCH, 29456 p. 2011.
  8. Inouye S, Takizawa T, Yamaguchi H. Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. J Antimicrobial Chemotherapy. 2001;47:565-573.
  9. Pavia CS, Pierre A, Nowakowski J. Antimicrobial activity of nicotine against a spectrum of bacterial and fungal pathogens. J Med Microbiology. 2000;49:675-676.
  10. Alagić S, Selekcija IS, Palić R, Stojanović G, Nikolić M. Chemical composition and antimicrobial activity of the essential oil of the oriental tobacco Yaka. J Essen Oil Res. 2002;14:230-232.
آمار
تعداد مشاهده مقاله: 1,094
تعداد دریافت فایل اصل مقاله: 887


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب