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کنترل بیولوژیکی سیاهک سخت جو توسط باکتری‌های آنتاگونیست | ||
| آفات و بیماری‌های گیاهی | ||
| دوره 74، شماره 2 - شماره پیاپی 82، اسفند 1385، صفحه 81-91 اصل مقاله (178.81 K) | ||
| نویسندگان | ||
| حسن رضا اعتباریان* ؛ مهرنوش محمدی فر؛ حسین علیزاده؛ اصغر زارعی سرابی | ||
| چکیده | ||
| در یک آزمایش صحرایی، کارایی شش استرین از باکتری Bacillus که از مزارع گندم استان‌های مرکزی، گلستان و مازندران و 11 استرین از باکتری فلورسنت از جنس Pseudomonas که از مزارع کرج و استان مرکزی جداسازی شده بود برای کنترل بیولوژیک بیماری سیاهک سخت جو (Ustilago hordei) با استفاده از طرح بلوک‌های کامل تصادفی و با 3 تکرار در مزرعه در روی جو رقم کارون در کویر مورد بررسی قرار گرفت. نتایج نشان داد که استرین‌های (B2) Bacillus lichniformis و B. cereus (B3) بیماری را کاملاً کنترل کردند. درصد آلودگی جو به این بیماری در تیمارهای Bacillus sp. (B1) ،B. cereus (B4) ، B. subtilis (53) و B. subtilis 71 بین 119/0 تا 617/0 متغیر بود و به طور معنی‌داری کمتر از شاهد آلوده بود (0.01 < P). در بین استرین‌های Pseudomonas استرین‌های Pseudomonas fluorescens bio V (C15)، P. fluorescens bio V (E2) ، P. fluorescens (D11) و P. fluorescens bio I (32) با 13/0 تا 207/0 درصد آلودگی بیشترین اثر را در کنترل بیماری داشتند. سایر استرین‌های Pseudomonas مورد آزمایش نیز در کنترل بیماری مؤثر بوده و با شاهد آلوده اختلاف معنی‌دار داشتند. | ||
| کلیدواژه‌ها | ||
| سیاهک سخت جو؛ Bacillus lichniformis؛ Bacillus cereus؛ Bacillus subtilis؛ Pseudomonas fluorescens؛ کنترل بیولوژیک؛ Ustilago hordei | ||
| عنوان مقاله [English] | ||
| Biological control of barley covered smut by bacterial antagonists | ||
| نویسندگان [English] | ||
| H. R. Etebarian؛ M. Mohammadifar؛ H. Alizadeh؛ A. ZAREI SARABI | ||
| چکیده [English] | ||
| Six strains of Bacillus which were isolated from wheat field of Markazi, Golestan and Mazandaran provinces and also 11 strains of Pseudomonas fluorescens isolated from wheat fields of Karaj and Markazi province were evaluated as potential biological agents for control of barley covered smut caused by Ustilago hordei in field. The randomized complete design with three replications was used for experiments. Results indicated that Bacillus lichniformis (B1) and B. cereus (B2) completely controlled barley covered smut. The percentage of infection in treatments with Bacillus sp (B2), B. cereus (B4), B. subtilis (53) and B. subtilis (71) ranged from 0.119 to 0.617 and were significantly less than infected control (p < 0.01). The best results were achived with Pseudomonas fluorescens bioV (C15), P. fluorescens bioV (E2), P. fluoresecns (D11) and P. fluoresecns bioI (32). The percentage of infection in these treatments were 0.207, 0.177, 0.133 and 0.18 respectively. Other strains of Pseudomonas were capable of reducing the incidence of covered smut and the percentage of infection significantly less than infected control. | ||
| کلیدواژه‌ها [English] | ||
| Barley covered smut, Bacillus lichniformis, Bacillus cereus, Bacillus subtilis, Pseudomonas fluorescens, Biological control, Ustilago hordei | ||
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