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Separation and Purification of Effective Ingredient of Galegine from Galega officinalis L. by Column Chromatography Tandemed with Molecularly Imprinted Polymer Enforced by Graphene Oxide (GO-MIP) Technique | ||
| Journal of Medicinal plants and By-product | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 06 آبان 1399 اصل مقاله (1.47 MB) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2020.341244.1168 | ||
| نویسندگان | ||
Maryam Azimi؛ Mazyar Ahmadi Golsefidi؛ Ali Varasteh Moradi   ؛ Mehdi Ebadi؛ Ramin Zafar Mehrabian
| ||
| Department of Chemistry, Faculty of Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran | ||
| چکیده | ||
| Galega officinalis L. (Papilionaceae) is a native plant from southeastern Europe and is used in the traditional medicinal system of Bulgaria, Italy, and India and is one of the medicinal plants that is used in the treatment of various diseases including diabetes. The extract of this plant has many effective compounds such as Galegine and chromatography is one of the most important methods for separating and purifying. Galegine is a guanidine alkaloid and a major compound isolated from G. officinalis to reduce the blood glucose levels. For this purpose, 10 g of dried Galga leaf was extracted with 50% ethanol-water mixture. In this study column chromatography tandemed with molecularly imprinted polymer technique was used to achieve this valuable material. Separation of extract components was performed by column chromatography using silica gel as stationary phase. A completely non-polar hexane solvent was used to elution the column, and then, the polarity of the solvent was increased with ethyl acetate. Then, the column was eluted with chloroform, methanol and finally, ethanol and water solvents and all the remaining components were exited out. Finally, eleven components were achieved. Thin-layer chromatography (TLC) was used to identify the components. Then, the molecularly imprinted polymer (Go-MIP) was synthesized for galegine, using of it as a template molecule, also Graphene Oxide, Methacrylic acid (MAA) as a functional monomer, Ethylene Glycol Dimethacrylate (EGDMA) as a cross-linker, Azobisisobutyronitrile (AIBN) as a reaction initiator, and acetonitrile as solvent were used. The Galegine in the extraction fraction of the column was purified by 0.1 g of GO-MIP. The concentration of the solutions was determinated before and after loading by HPLC. The results showed that using the chromatographic column led to removing significantly the interfacial compounds (the amount of Galegine adsorbed by GO-MIP from the extract was 193 µg/g. Whereas, the amount of Galegine absorbed by GO-MIP obtaining from the column output fraction was 330 µg/g) and Galegine was extracted and separated with a higher purity percentage from the plant extract. On the other hand, increased the efficiency of the mentioned technique for purifying of this compound up to seventeen fold. | ||
| کلیدواژه‌ها | ||
| Galega officinalis L؛ Galegine؛ column chromatography؛ molecularly imprinted polymer؛ separation | ||
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آمار تعداد مشاهده مقاله: 53 تعداد دریافت فایل اصل مقاله: 75 |
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