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غربالگری مقاومت به قارچ Verticillium dahliae در ارقام امیدبخش و کلکسیون ژنوتیپهای بومی زیتون ایران | ||
| آفات و بیماری های گیاهی | ||
| مقاله 4، دوره 88، شماره 1 - شماره پیاپی 110، شهریور 1399، صفحه 39-51 اصل مقاله (622.98 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2020.127330.1300 | ||
| نویسندگان | ||
| حسین جعفری* 1؛ ندا زند2؛ مهدی طاهری3؛ علی اصغر زینانلو4؛ شمس الله نجفی5 | ||
| 1دانشیار، بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان زنجان، سازمان تحقیقات، آموزش و ترویج کشاورزی، | ||
| 2محقق، بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان زنجان، سازمان تحقیقات، آموزش و ترویج کشاورزی، زنجان، | ||
| 3بخش تحقیقات خاک و آب، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان زنجان، سازمان تحقیقات، آموزش و ترویج کشاورزی، زنجان، ایران | ||
| 4پژوهشکده میوه‌های معتدله و سردسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران. | ||
| 5محقق، بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان زنجان، سازمان تحقیقات، آموزش و ترویج کشاورزی، زنجان، ایران. | ||
| چکیده | ||
| بیماری پژمردگی ورتیسیلیومی در اثر قارچ Verticillium dahliae یکی از مخربترین عوامل بیماریزای زیتون دردنیا ومناطق مختلف زیتون‌کاری ایران محسوب می‌شود. در این تحقیق عکس‌العمل 15 رقم امیدبخش و 15 ژنوتیپ بومی زیتون نسبت به قارچ عامل بیماری بررسی شد. نهال‌های نه ماهه زیتون برای ارزیابی کمی قارچ عامل بیماری و تعیین میزان حساسیت یا مقاومت نسبت به بیماری با روش فرو بردن ریشه در زاد مایه انجام شد. میزان افزایش کمی DNA قارچ موجود در نهالها با استفاده از تکنیک Real time PCR و سیستم SYBR Green همراه با آغازگرهای اختصاصی برای ژن بتا توبولین2 در فواصل زمانی صفر،2، 15، 45 و80 روز پس از مایه زنی، ارزیابی شد. ژنوتیپهای QG-11، DD2-1، PS-5 و رقم مانزانیلا به‌ترتیب بالاترین حساسیت را نسبت به سایر ارقام و ژنوتیپها در برابر بیماری داشتنند. ژنوتیپهای KH-13، TTS-1، کد 5 لرستان و DS-5 به‌ترتیب کم‌ترین غلظت DNA قارچ در DNA کل ریشه را به خود اختصاص دادند. علاوه بر این، در این تحقیق مشخص گردید نتایج روش متعارف ارزیابی مقاومت ارقام زیتون مبتنیبر ثبت علایم ظاهری بیماری با نتایج حاصل از کمیت سنجی قارچ با استفاده از Real time PCR دارای هم‌پوشانی نسبتاً بالایی است به‌طوری که ارقامی که در روش ارزیابی متعارف جزء ارقام مقاوم یا بسیار حساس طبقه‌بندی شدند، درروش ارزیابی ملکولی نیز در گروه‌های مشابهی قرار گرفتند. با توجه به‌دقت وحساسیت بالای روش ارزیابی ملکولی می‌توان از روش اخیر برای غربالگری منابع مختلف زیتون برای مقاومت به بیماری پژمردگی ورتیسیلیومی استفاده کرد. | ||
| کلیدواژه‌ها | ||
| ارزیابی کمی؛ ارقام زیتون؛ مقاومت؛ Real time PCR | ||
| عنوان مقاله [English] | ||
| Screening of promising olive cultivars and local genotypes for resistance to Verticillium dahliae in Iranian collection | ||
| نویسندگان [English] | ||
| Hossein Jafary1؛ Neda Zand2؛ Mahdi Taheri3؛ Aliasghar Zeinanloo4؛ Shamsollah Najafi5 | ||
| 1Associate Professor of Plant Protection Research Department, Zanjan Agricultural and Natural Resources Research and Education Center, AREEO, Zanjan, Iran. | ||
| 2Researcher, Plant Protection Research Department, Zanjan Agricultural and Natural Resources Research and Education Center, AREEO, Zanjan, Iran. | ||
| 3Associate Professor, Soil and Water Research Department, , Zanjan Agricultural and Natural Resources Research and Education Center, AREEO, Zanjan, Iran. | ||
| 4Horticultural Sciences Research Institute, AREEO, Karaj, Iran. | ||
| 5Researcher, Plant Protection Research Department, Zanjan Agricultural and Natural Resources Research and Education Center, AREEO, Zanjan, Iran. | ||
| چکیده [English] | ||
| Verticilliumwilt of olive (VWO) caused by fungus Verticillium dahliae, is one of the most important diseases of olive worldwide. The response of 15 promising olive cultivars and 15 local varieties was evaluated to the fungus. The olive saplings of each genotype were inoculated with suspension of the fungus using root dip method. The degree of susceptibility/resistance of each genotype was evaluated based on both morphological feature and quantification of fungus in the root system of different genotypes. Total DNA was extracted from root of saplings at different sampling intervals: 0, 2, 12, 45 and 80 days post inoculation. The gradual increase for DNA of fungus in saplings was quantified by Real-time PCR technique and utilizing of SYBR Green system with specific primer pairs for ß-tubulin2 gene. Based on both morphological and quantitative Real-time PCR assays: QG-11, DD2-1, treeNo2, PS-5 and Manzanilla were highly susceptible and KH-13, TTS-1, DS-5 code 5 Lorestan and DS-5 were evaluated as resistant genotypes to the VWO. Moreover, we found parallel results for conventional method of screening (based on disease symptoms) and quantification of DNA in root system of olive by Real-time PCR technique. Olive genotypes evaluated as highly susceptible or resistant to the disease in conventional method showed the similar reaction to the fungus in Q-PCR method. This may suggest that conventional method of evaluation for resistance could be replaced by Q-PCR molecular method in high thropout screening of olive germplasm. | ||
| کلیدواژه‌ها [English] | ||
| Olive cultivars, Quantitative assessment, Real time PCR, Resistance | ||
| مراجع | ||
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