Abdollahi, Z, Zeinoaldini, S, Zhandi, M, Towhidi, A, Baghshahi, H. (1400). Supplementation of Plant-Based Cryopreservation Medium with Folic Acid Conserves the Quality of Bulk Post-Thawed Spermatozoa. سامانه مدیریت نشریات علمی, 76(3), 553-559. doi: 10.22092/ari.2020.351865.1541
Z Abdollahi; S Zeinoaldini; M Zhandi; A Towhidi; H Baghshahi. "Supplementation of Plant-Based Cryopreservation Medium with Folic Acid Conserves the Quality of Bulk Post-Thawed Spermatozoa". سامانه مدیریت نشریات علمی, 76, 3, 1400, 553-559. doi: 10.22092/ari.2020.351865.1541
Abdollahi, Z, Zeinoaldini, S, Zhandi, M, Towhidi, A, Baghshahi, H. (1400). 'Supplementation of Plant-Based Cryopreservation Medium with Folic Acid Conserves the Quality of Bulk Post-Thawed Spermatozoa', سامانه مدیریت نشریات علمی, 76(3), pp. 553-559. doi: 10.22092/ari.2020.351865.1541
Abdollahi, Z, Zeinoaldini, S, Zhandi, M, Towhidi, A, Baghshahi, H. Supplementation of Plant-Based Cryopreservation Medium with Folic Acid Conserves the Quality of Bulk Post-Thawed Spermatozoa. سامانه مدیریت نشریات علمی, 1400; 76(3): 553-559. doi: 10.22092/ari.2020.351865.1541
Supplementation of Plant-Based Cryopreservation Medium with Folic Acid Conserves the Quality of Bulk Post-Thawed Spermatozoa
Department of Animal Science, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده
The current study evaluated the effects of cryopreservation medium supplementation with folic acid as an antioxidant on post-thawed semen quality in bulk. Semen samples were collected from four proved Iranian Mahabadi bulls and diluted in extender containing 1.5% soybean lecithin. The diluted semen was assigned into six parts and supplemented with different doses of folic acid as follows: FA0 (extender without folic acid), FA0.05, FA0.1, FA0.2, FA0.4, and FA0.8 (extenders containing 0.05, 0.1, 0.2, 0.4, and 0.8 mM folic acid, respectively). Then, the semen samples were cryopreserved in liquid nitrogen. Sperm motility and velocity parameters, membrane integrity, abnormal morphology, viability, and lipid peroxidation were evaluated after thawing. In the results, FA0.05 presented higher (p≤0.05) total motility, progressive motility, membrane integrity, and viability and lower lipid peroxidation compared to other groups. Abnormal morphology was not affected (p>0.05) by treatments. In conclusion, supplementation of cryopreservation medium with 0.05 mM folic acid is a helpful method to conserve the quality of post-thawed semen in bulk.
استفاده از فولیک اسید در رقیق کننده انجماد اسپرم بر پایه گیاهی کیفیت اسپرم بز را پس از فرآیند یخ-گشایی حفظ میکند
چکیده [English]
هدف از این مطالعه ارزیابی اثر استفاده از فولیک اسید به عنوان یک آنتی اکسیدان در رقیق کننده انجماد بر کیفیت اسپرم بز پس از یخگشایی بوده است. نمونه اسپرم از چهار راس بز مهابادی جمع آوری و در رقیق کننده حاوری 5/1 درصد لسیتین سویا رقیق شد. اسپرم رقیق شده به شش قسمت تقسیم شده و دوزهای مختلف اسید فولیک (0، 05/0، 1/0، 2/0، 4/0 و 8/0) را دریافت کردند. سپس نمونه ها در ازت مایع منجمد شدند. جنبایی، شاخصهای سرعت، سلامت غشا، موفولوژی غیر طبیعی، زندهمانی و پراکسیداسیون لیپیدهای غشایی پس از یخگشایی ارزیابی شدند. در نتیجه تیمار دریافت کننده 05/0 میلی مولار اسید فولیک مقدار بالاتر (P≤0.05) جنبایی کل، جنبایی پیشرونده، سلامت غشا، زندهمانی و مقدار پایینتر (P≤0.05) پراکسیداسیون لیپیدی را در مقایسه با سایر گرئهها نشان داد. استفاده از تیمارهای محتلف تاثیری (P>0.05) بر مورفولوژی غیر نرمال نداشت. بنابراین افزودن 05/0 میلی مولار اسید فولیک به محیط انجماد اسپرم بز روش مناسبی برای حفظ کیفیت اسپرم پس از یخگشایی میباشد.
کلیدواژهها [English]
بز, حفاظت انجمادی, فولیک اسید, اسرم, لسیتین سویا
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