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بررسی تأثیر کیفیت اولیه بذر و شرایط انبارداری بر تغییرات بیوشیمیایی و فیزیولوژیکی بذر سویا | ||
| علوم و فناوری بذر ایران | ||
| مقاله 8، دوره 9، شماره 2 - شماره پیاپی 18، شهریور 1399، صفحه 101-118 اصل مقاله (1.01 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.123739.1232 | ||
| نویسندگان | ||
| سامان شیدایی* ؛ آیدین حمیدی؛ حسین صادقی؛ بیتا اسکویی | ||
| عضو هیأت علمی، مؤسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| به‌منظور ارزیابی تغییرات بیوشیمیایی در طی زوال بذر سویا، تحقیقی به‌صورت آزمایش فاکتوریل بر پایه طرح کامل تصادفی اجرا گردید. فاکتورهای آزمایش شامل قوه نامیه در دو سطح قوه نامیه 80% و 90%، سه میزان رطوبت اولیه بذر شامل رطوبت پایین (10%)، رطوبت متوسط (12%) و رطوبت بالا (14%) و دو شرایط انبارکردن شامل انبار متداول نگهداری بذر سویا در مغان و انبار با دما و رطوبت کنترل شده در نظر گرفته شد. صفات درصد جوانه‌زنی، بنیه، میزان پروتئین، قندهای محلول، مالون دی آلدئید، فعالیت آنزیم‌های کاتالاز و پراکسیداز مورد سنجش قرار گرفتند. بررسی تغییرات بیوشیمیایی بذرهای زوال یافته در طی انبار کردن نشان داد که با افزایش زوال بذرها میزان قندهای محلول و درصد پروتئین کاهش یافت و نگهداری بذرها در شرایط نامناسب باعث کاهش معنی‌دار در میزان قندهای محلول و پروتئین کل بذر شد. همچنین با افزایش رطوبت بذر که افزایش زوال بذر را در پی داشت از محتوای قندهای محلول و پروتئین کل به-طور معنی‌داری کاسته شد. بین بذرهای با قوه نامیه اولیه متفاوت تفاوت معنی‌داری دیده شد و بذرهای با قوه نامیه اولیه 90 درصد به‌طور معنی‌داری محتوای قندهای محلول و درصد پروتئین بالاتری داشتند. همچنین با اندازه‌گیری میزان مالون دی-آلدئید و فعالیت آنزیم‌های کاتالاز و پراکسیداز مشخص گردید بین میزان زوال بذر با میزان مالون دی‌آلدئید و فعالیت آنزیم‌های تنظیف همبستگی بالایی مشاهده گردید. همراه با کاهش میزان جوانه‌زنی و کیفیت بذر سویا پس از انبارکردن، میزان پراکسیداسیون چربی‌ها افزایش و زوال بذر از فعالیت آنزیم‌های کاتالاز و پراکسیداز در طی انبارکردن کاست. | ||
| کلیدواژه‌ها | ||
| انبارداری؛ پروتئین؛ کاتالاز؛ مالون‌دی‌آلدئید؛ قندهای محلول | ||
| عنوان مقاله [English] | ||
| Evaluation of initial seed quality and storage conditions on biochemical and physiological changes of soybean seeds | ||
| نویسندگان [English] | ||
| S. Sheidaei؛ Aidin Hamidi؛ Hossein Sadeghi؛ Bita Oskouei | ||
| Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | ||
| چکیده [English] | ||
| A factorial experiment was conducted to evaluate the biochemical changes during the soybean seed deterioration based on a completely randomized design. The treatments consisted of germination in two levels of minimum and high quality, three initial seed moisture content (low, medium and high moisture), and two storage conditions were considered including the common storage in Moghan and the controlled storage condition. Indices of germination percentage, vigor and biochemical changes including protein content, soluble sugars, malondialdehyde, catalase and peroxidase enzymes were evaluated. The study of biochemical changes of deteriorated seeds during the storage showed that, with the increase of seeds’ deterioration, the soluble sugars and protein percentage decreased, as well. Moreover, seeds’ storage in poor condition caused a significant decrease in seeds’ soluble sugar and total protein content. Also, the increase of seed moisture was followed by further seed deterioration that significantly decreased the content of soluble sugars and total protein. Significant differences were observed between seeds with different initial germinations and they had significantly higher content of soluble sugars and protein percentages. Furthermore, measuring the amount of malondialdehyde and the activity of catalase and peroxidase enzymes indicated that there exists a significant correlation between the degree of seed deterioration with the level of malondialdehyde and the activity of scavenging enzymes. Overall, along with the decrease of germination and the quality of soybean seeds after the storage, the amount of lipids peroxidation increased and the seed deterioration decreased the activity of catalase and peroxidase enzymes during the storage. | ||
| کلیدواژه‌ها [English] | ||
| Catalase, Soluble sugar, Storage, Malondialdehyde | ||
| مراجع | ||
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