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مطالعه اثر پیری تسریع شده و اسید جیبرلیک بر خصوصیات جوانه زنی، پر اکسیداسیون لیپیدها، پروتئین محلول، فنل کل و هدایت الکتریکی بذر گندم (رقم پیشتاز) (Triticum aestivum L.) | ||
| علوم و فناوری بذر ایران | ||
| مقاله 4، دوره 9، شماره 2 - شماره پیاپی 18، شهریور 1399، صفحه 49-60 اصل مقاله (1.26 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2020.115718.1136 | ||
| نویسندگان | ||
| زهرا محدث اردبیلی1؛ حسین عباسپور2؛ رضا توکل افشاری* 3؛ سید محسن نبوی کلات4 | ||
| 1گروه زیست شناسی، واحددامغان، دانشگاه آزاد اسلامی، دامغان، ایران | ||
| 2گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 3استادگروه زراعت، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| 4گروه زراعت ، ,واحدمشهد، دانشگاه ازاداسلامی، مشهد،ایران | ||
| چکیده | ||
| به‌منظور مطالعه اثر پیری تسریع شده و پرایمینگ بذر با اسید جیبرلیک بر خصوصیات جوانه‌زنی، پر اکسیداسیون لیپیدها، پروتئین محلول، فنل کل و هدایت الکتریکی بذر گندم (رقم پیشتاز) یک آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه تکنولوژی بذر گروه زراعت دانشکده کشاورزی دانشگاه فردوسی مشهد در سال 1395 انجام شد. عوامل آزمایش شامل پیری بذرتحت شرایط دمای 40 درجه سانتی گرادو 100% رطوبت در سه سطح [زوال کم (4 روز)، زوال متوسط (6 روز) و زوال زیاد (7 روز)] و پرایمینگ بذر با اسید جیبرلیک در 4 سطح (0، 25، 50 و 100 پی‌پی-ام) بود. نتایج تجزیه واریانس نشان داد که اثر پیری بذر، پرایمینگ با اسید جیبرلیک و اثر متقابل دو عامل بر تمامی صفات اندازه‌گیری شده معنی‌دار بود. بر اساس نتایج مقایسه میانگین صفات با افزایش مدت‌زمان پیری درصد و سرعت جوانه‌زنی، پروتئین محلول و فنل کل کاهش یافت؛ اما میزان مالون‌دی‌آلدئید و هدایت الکتریکی به علت آسیب وارده به غشای سلولی با افزایش سطوح پیری افزایش یافت. بررسی اثرات متقابل دو عامل نشان داد که پرایمینگ با اسید جیبرلیک اثرات منفی پیری بذر بر تمامی صفات اندازه‌گیری شده را کاهش می‌دهد. | ||
| کلیدواژه‌ها | ||
| جیبرلیک اسید؛ درصد جوانه‌زنی؛ زوال؛ سرعت جوانه‌زنی؛ مالون دی آلدئید | ||
| عنوان مقاله [English] | ||
| The study on effect of accelerated aging and gibberellic acid on germination characteristics, lipids peroxidation, soluble protein, total phenol and electrical conductivity of wheat seed (Pishtaz Cultivar) (Triticum aestivum L.) | ||
| نویسندگان [English] | ||
| zahra Mohaddes Ardebili1؛ Hossein Abbaspour2؛ Reza Tavakkol afshari3؛ Seid Mohsen Nabavi Kalat44 | ||
| 1Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. | ||
| 2Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91779-48974, Mashhad, Iran | ||
| 4Department of Agronomy, Mashhad Branch, Islamic Azad University, Mashhad, Iran. | ||
| چکیده [English] | ||
| In order to study the effect of accelerated aging and priming with gibberellic acid on germination characteristics, lipids peroxidation, soluble protein, total phenol and electrical conductivity of wheat seed (Pishtaz Cultivar) an experiment in factorial laid out completely randomized design with three replications was conducted at Seed Technology Laboratory, Department of Agronomy, Faculty of Agriculture, Ferdowsi University, Mashhad, Iran, in 2016. Factors were included of seed aging(100% relative humidity at 40°C) in three levels [low deterioration (4 days), medium deterioration (6 days) and high deterioration (7 days)] and priming with gibberellic acid in four levels (0, 25, 50 and 100 ppm). Analysis of variance showed that the effect of seed aging, priming with gibberellic acid and interaction between two factors on all traits were significant. Based on the means comparison of the traits germination percentage, germination rate, soluble protein and total phenol decreased with increasing aging time. But, the level of malondyaldeid and electrical conductivity increased due to damage to cell membrane with increasing aging time. Investigation of interaction between two factors showed that the priming with gibberellic acid reduced the negative effects of seed aging on all traits. | ||
| کلیدواژه‌ها [English] | ||
| Deterioration, Gibberellic acid, Germination percentage, Germination speed, Malondyaldeid | ||
| مراجع | ||
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