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ارزیابی ترکیب‌های فنولیک و فعالیت آنتی‌اکسیدانی در برگ گونه‌های مختلف گل ماهور (Verbascum L.) در استان آذربایجان‌غربی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 36، شماره 3 - شماره پیاپی 101، مرداد و شهریور 1399، صفحه 466-482 اصل مقاله (401.47 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.128276.2663 | ||
| نویسندگان | ||
| سونیا امینی1؛ عباس حسنی* 2؛ ابوالفضل علیرضالو3؛ رامین ملکی4 | ||
| 1دانشجوی دکترا، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 2استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 3استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 4استادیار، گروه پژوهشی کروماتوگرافی، جهاد دانشگاهی آذربایجان‌غربی، ارومیه، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.36.466.101.3.1575.1610 گل ماهور یا خرگوشک (Verbascum L.) گیاهی علفی، دوساله، دارویی و متعلق به تیره گل‌میمون (Scrophulariaceae) می‌باشد. از گل‌های این گیاه برای درمان ناراحتی‌های دستگاه تنفس استفاده می‌شود. در این تحقیق تنوع ژنتیکی گونه‌های مختلف گل ماهور جمع‌آوری‌شده از مناطق مختلف استان آذربایجان‌غربی براساس برخی نشانگرهای فیتوشیمیایی در راستای اهلی‌سازی این گیاه ارزشمند مورد بررسی قرار گرفت. تنوع فیتوشیمیایی گونه‌های مختلف گل ماهور براساس محتوای کاروتنوئید، بتا‌کاروتن، مقدار فنل کل، فلاونوئید کل، فعالیت آنتی‌اکسیدانی (روش FRAP و DPPH) و ترکیب‌های فنلی (گالیک اسید، کافئیک اسید، کلروژنیک اسید، روتین، کوماریک اسید، کوئرسیتین، سینامیک اسید و آپی‌ژنین) به روش HPLC در اندام برگ ارزیابی گردید. نتایج نشان داد که نوع گونه تأثیر معنی‌داری در سطح احتمال 1% بر میزان کاروتنوئید، بتا‌کاروتن، فنل، فلاونوئید کل، فعالیت آنتی‌اکسیدانی و ترکیب‌های فنلی داشته است. بیشترین میزان کاروتنوئید کل (mg/g DW 76.27)، بتا‌کاروتن (mg/100 g DW ۸۸/۵)، فنل کل (mg GAE/g DW 32.83)، فلاونوئید کل (mg QE/g DW 7.13) و فعالیت آنتی‌اکسیدانی به روش DPPH (µg AA/mL 34.66) در برگ‌های گونه V. sinuatum C. Kochمشاهده شد. نتایج نشان داد که برگ‌های گونه V. erianthum Benth. بیشترین میزان ترکیب‌های کلروژنیک اسید، کوئرسیتین، سینامیک اسید و آپی‌ژنین را دارد، در حالی‌که بیشترین مقدار گالیک اسید و کافئیک اسید در V. saccatum مشاهده شد. درمجموع یافته‌های این تحقیق نشان داد که تنوع وسیعی از گونه‌های گل ماهور در استان آذربایجان‌غربی وجود دارد. گونه‌های V. erianthum و V. saccatum دارای خصوصیات فیتوشیمیایی منحصر به‌فردی بودند که می‌توان از آنها برای طراحی برنامه‌های اصلاحی و نیز بهره‌برداری در صنایع داروسازی استفاده نمود. | ||
| کلیدواژه‌ها | ||
| تنوع فیتوشیمیایی؛ خرگوشک (Verbascum L.)؛ ترکیب‌های فنلی؛ فنل کل؛ فلاونوئید کل | ||
| عنوان مقاله [English] | ||
| Evaluation of phenolic compounds and antioxidant activity in leaves of different different Verbascum L. species in West Azerbaijan province | ||
| نویسندگان [English] | ||
| S. Amini1؛ A. Hassani2؛ A. Alirezalu3؛ R. Maleki4 | ||
| 1Ph.D. student, Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 2Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 3Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 4Research Department of Chromatography, Iranian Academic Center for Education, Culture and Research (ACECR), Urmia, Iran | ||
| چکیده [English] | ||
| Mullein (Verbascum L.) is a biennial herbaceous medicinal plant belonging to the Scrophulariaceae family. The flowers of this plant are used to treat respiratory disorders. In this study, the genetic diversity of different mullein species from different regions of West Azerbaijan province was investigated based on some phytochemical markers to domesticate this valuable plant. Phytochemical diversity among different species of mullein was evaluated based on the content of carotenoid, beta-carotene, total phenol, total flavonoid, and antioxidant activity (by FRAP and DPPH methods), and identification of phenolic compounds (gallic acid, caffeic acid, chlorogenic acid, rutin, coumaric acid, quercetin, cinnamic acid, and apigenin) by HPLC in leaf. The results showed that all phytochemical characteristics studied were significantly different among species (P<0.01). The highest content of carotenoid (76.27 mg g-1 DW), beta-carotene (5.88 mg 100g-1 DW), total phenol (32.83 mg GAE g-1 DW), total flavonoid (7.13 mg QE g-1 DW) and antioxidant activity by DPPH method (34.66 µg AA mL-1) was observed in V. sinuatum. The results showed that V. erianthum leaves had the highest content of chlorogenic acid, quercetin, cinnamic acid, and apigenin, while the highest content of gallic acid and caffeic acid was observed in V. saccatum. In general, the findings of this research showed that there was a wide variation of mullein species in West Azerbaijan province. Species V. erianthum and V. saccatum had unique phytochemical properties that can be used to design breeding programs and use them in the pharmaceutical industry. | ||
| کلیدواژه‌ها [English] | ||
| Phytochemical diversity, mullein (Verbascum L.), phenolic compounds, total phenol, total flavonoid | ||
| مراجع | ||
|
- Agbo, M.O., Uzor, P.F., Nneji, U.N.A., Odurukwe, C.U.E., Ogbatue, U.B. and Mbaoji, E.C., 2015. Antioxidant, total phenolic and flavonoid content of selected Nigerian medicinal plants. Dhaka University Journal of Pharmaceutical Sciences, 14(1): 35-41. - Alirezalu, A., Ahmadi, N., Salehi, P., Sonboli, A., Ayyari, M. and Hatami-Maleki, H., 2015. Antioxidant capacity in different organs of Hawthorn various species (Crataegus spp.). Journal of Food Research, 25(2): 325-338. - Amini, S., Hassani, A., Alirezalu, A. and Maleki, R., 2018. Investigation of genetic diversity among Verbascum species in West Azerbaijan province by morphological and phytochemical markers. Journal of Plant Production Research, 24(3): 123-142. - Armatu, A., Bodirlau, R., Nechita, C., Niculaua, M., Teaca, C., Ichim, M. and Spiridon, I., 2011. Characterization of biological active compounds from Verbascum phlomoides by chromatography techniques. I. Gas chromatography. Romanian Biotechnological Letters, 16(4): 6297-6304. - Benzie, I.F. and Strain, J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1): 70-76. - Bernath, J., 2002. Strategies and recent achievements in selection of medicinal and aromatic plants. Acta Horticulturae, 576: 115-128. - Burits, M. and Bucar, F., 2000. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14(5): 323-328. - Carvalho, L., Carvalho, J., Faustino, R., Kaser, I., Lima, V. and Sousa, D., 2015. Variability of total carotenoids in C. moschata genotypes. Chemical Engineering Transactions, 44: 247-252. - Ebrahimzadeh, M., Hosseinimehr, S., Hamidinia, A. and Jafari, M., 2008. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacologyonline, 1: 7-14. - Ghasemi, G., Fattahi, M. and Alirezalu, A., 2017. Evaluation of diversity in some phytochemical characteristics among flower extract of wild-growing populations of Rheum ribes L. in Iran. Eco-Phytochemical Journal of Medicinal Plants, 4(4): 49-61. - Grigore, A., Colceru-Mihul, S., Litescu, S., Panteli, M. and Rasit, I., 2013. Correlation between polyphenol content and anti-inflammatory activity of Verbascum phlomoides (Mullein). Pharmaceutical Biology, 51(7): 925-929. - He, S., Ohm, H. and Mackenize, S., 1992. Detection of DNA sequence polymorphisms among wheat varieties. Theoretical and Applied Genetics, 84: 573-578. - Javanmardi, J., Stushnoff, C., Locke, E. and Vivanco, J., 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry, 83(4): 547-550. - Kahraman, C., Akdemir, Z.S. and Tatlı, I.I., 2010. Promising cytotoxic activity profile, biological activities and phytochemical screening of Verbascum L. species. Medicinal and Aromatic Plant Science and Biotechnology, 6(2): 63-75. - Karamian, R. and Ghasemlou, F., 2013. Total phenolic content, antioxidant and antibacterial activities of three Verbascum species from Iran. Journal of Medicinal Plants and By-Products, 2(1): 43-51. - Kheiri, S., 2009. Identification of breeding system of some species of Verbascum (Scrophulariaceae) in north-west of Iran on the basis of the ratio of pollen to ovule number. Journal of Biology, Islamic Azad University, 4(2): 67-74. - Khalighi-Sigaroodi, F., Ahvazi, M., Ebrahimzadeh, H., Rahimifard, N., 2013 Chemical composition of the essential oil and antioxidant activities, total phenol and flavonoid content of the extract of Nepeta pogonosperma. Journal of Medicinal Plants, 4 (48):185-198. - Kharazian, N. and Rahiminejad, M., 2009. Study of phenolic constituents of Triticum L. (Poaceae) species in Iran. Iranian Journal of Science and Technology, 33(4): 309-315. - Kumar, G.P., Kumar, R., Badere, R. and Singh, S.B., 2011. Antibacterial and antioxidant activities of ethanol extracts from trans himalayan medicinal plants. European Pharmacognosy Journal, 17(2): 66-69. - Lichtenthaler, H.K., 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology, 148: 350-382. - Martz, F., Jaakola, L., Julkunen-Tiitto, R. and Stark, S., 2010. Phenolic composition and antioxidant capacity of bilberry (Vaccinium myrtillus) leaves in Northern Europe following foliar development and along environmental gradients. Journal of Chemical Ecology, 39(9): 1017-1028. - Mihailović, V., Kreft, S., Benković, E.T., Ivanović, N. and Stanković, M.S., 2016. Chemical profile, antioxidant activity and stability in stimulated gastrointestinal tract model system of three Verbascum species. Industrial Crops and Products, 89: 141-151. - Nagata, M., 2009. A simple spectrophotometric method for the estimation of β-carotene content in spinach acetone extracts. Bulletin of the National Institute of Vegetable and Tea Science, 8: 1-5. - Nofouzi, K., 2015. Study on the antioxidant activity and in vitro antifungal activity of Verbascum speciosum methanolic extract. Journal of Mycology Research, 2(2): 97-103. - Nowak, R. and Gawlik-Dziki, U., 2007. Polyphenols of Rosa L. leaves extracts and their radical scavenging activity. Zeitschrift für Naturforschung, 62(1-2): 32-38. - Peksel, A., Arisan-Atac, I. and Yanardag, R., 2010. Evaluation of antioxidant and antiacetylcholinesterase activities of the extracts of Pistacia atlantica Desf. Leaves. Journal of Food Biochemistry, 34(3): 451-476. - Riaz, M., Zia-Ul-Haq, M. and Jaafar, H.Z., 2013. Common mullein, pharmacological and chemical aspects. Revista Brasileira de Farmacognosia, 23(6): 948-959. - Rigane, G., Ben, Y., Ghazghazi, H. and Ben, S., 2013. Investigation into the biological activities and chemical composition of Calendula officinalis L. growing in Tunisia. International Food Research Journal, 20(6): 3001-3007. - Saboura, A., Ahmadi, A., Zeynali, A. and Parsa, M., 2014. Comparison between the contents of phenolic and flavonoid compounds and aerial part antioxidant activity in Scutellaria pinnatifida in two North Iranian populations. Journal of Rafsanjan University of Medical Sciences, 13(3): 249-266. - Safi, Z., Saeidi, K., Lorigooini, Z. and Shirmardi, H.A., 2016. Evaluation of total phenols and antioxidant activity of Mullein (Verbascum songaricum) ecotypes. Journal of Shahrekord Uuniversity of Medical Sciences, 17(6): 68-75. - Seal, T., 2016. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India. Journal of Applied Pharmaceutical Science, 6(02): 157-166. - Selseleh, M., Hadian, J., Nejad Ebrahimi, S., Sonboli, A., Georgiev, M.I. and Mirjalili, M.H., 2019. Metabolic diversity and genetic association between wild populations of Verbascum songaricum (Scrophulariaceae). Industrial Crops and Products, 137: 112-125. - Sytar, O., Hemmerich, I., Zivcak, M., Rauh, C. and Brestic, M., 2016. Comparative analysis of bioactive phenolic compounds composition from 26 medicinal plants. Saudi Journal of Biological Sciences, 25: 631-641. - Tatli, I. and Akdemir, Z., 2004. Chemical constituents of Verbascum L. species. Fabad Journal of Pharmaceutical Sciences, 29: 93-107. - Tohma, H., Köksal, E., Kılıç, Ö., Alan, Y., Yılmaz, M.A., Gülçin, I., Bursal, E. and Alwasel, S.H., 2016. RP-HPLC/MS/MS analysis of the phenolic compounds, Antioxidant and antimicrobial activities of Salvia L. species. Antioxidants, 5(4): 38. - Turke, A.U. and Gurel, E., 2005. Common mullein (Verbascum thapsus L.): recent advances in research. Phytotherapy Research, 19(9): 733-739. - Zhelev, I., Dimitrova-Dyulgerova, I., Belkinova, D. and Mladenov, R., 2013. Content of phenolic compounds in the genus Carduus L. from Bulgaria. Ecologia Balkanica, 5(2): 14-20. - Žugić, A., Đorđević, S., Arsić, I., Marković, G., Živković, J., Jovanović, S. and Tadić, V., 2014. Antioxidant activity and phenolic compounds in 10 selected herbs from Vrujci Spa, Serbia. Industrial Crops and Products, 52: 519-527. | ||
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