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ارزیابی ترکیب‌های فنولیک و فعالیت آنتی‌اکسیدانی در برگ گونه‌های مختلف گل ماهور (Verbascum L.) در استان آذربایجان‌غربی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 36، شماره 3 - شماره پیاپی 101، مرداد و شهریور 1399، صفحه 466-482 اصل مقاله (401.47 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.128276.2663 | ||
| نویسندگان | ||
| سونیا امینی1؛ عباس حسنی* 2؛ ابوالفضل علیرضالو3؛ رامین ملکی4 | ||
| 1دانشجوی دکترا، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 2استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 3استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 4استادیار، گروه پژوهشی کروماتوگرافی، جهاد دانشگاهی آذربایجان‌غربی، ارومیه، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.36.466.101.3.1575.1610 گل ماهور یا خرگوشک (Verbascum L.) گیاهی علفی، دوساله، دارویی و متعلق به تیره گل‌میمون (Scrophulariaceae) می‌باشد. از گل‌های این گیاه برای درمان ناراحتی‌های دستگاه تنفس استفاده می‌شود. در این تحقیق تنوع ژنتیکی گونه‌های مختلف گل ماهور جمع‌آوری‌شده از مناطق مختلف استان آذربایجان‌غربی براساس برخی نشانگرهای فیتوشیمیایی در راستای اهلی‌سازی این گیاه ارزشمند مورد بررسی قرار گرفت. تنوع فیتوشیمیایی گونه‌های مختلف گل ماهور براساس محتوای کاروتنوئید، بتا‌کاروتن، مقدار فنل کل، فلاونوئید کل، فعالیت آنتی‌اکسیدانی (روش FRAP و DPPH) و ترکیب‌های فنلی (گالیک اسید، کافئیک اسید، کلروژنیک اسید، روتین، کوماریک اسید، کوئرسیتین، سینامیک اسید و آپی‌ژنین) به روش HPLC در اندام برگ ارزیابی گردید. نتایج نشان داد که نوع گونه تأثیر معنی‌داری در سطح احتمال 1% بر میزان کاروتنوئید، بتا‌کاروتن، فنل، فلاونوئید کل، فعالیت آنتی‌اکسیدانی و ترکیب‌های فنلی داشته است. بیشترین میزان کاروتنوئید کل (mg/g DW 76.27)، بتا‌کاروتن (mg/100 g DW ۸۸/۵)، فنل کل (mg GAE/g DW 32.83)، فلاونوئید کل (mg QE/g DW 7.13) و فعالیت آنتی‌اکسیدانی به روش DPPH (µg AA/mL 34.66) در برگ‌های گونه V. sinuatum C. Kochمشاهده شد. نتایج نشان داد که برگ‌های گونه V. erianthum Benth. بیشترین میزان ترکیب‌های کلروژنیک اسید، کوئرسیتین، سینامیک اسید و آپی‌ژنین را دارد، در حالی‌که بیشترین مقدار گالیک اسید و کافئیک اسید در V. saccatum مشاهده شد. درمجموع یافته‌های این تحقیق نشان داد که تنوع وسیعی از گونه‌های گل ماهور در استان آذربایجان‌غربی وجود دارد. گونه‌های V. erianthum و V. saccatum دارای خصوصیات فیتوشیمیایی منحصر به‌فردی بودند که می‌توان از آنها برای طراحی برنامه‌های اصلاحی و نیز بهره‌برداری در صنایع داروسازی استفاده نمود. | ||
| کلیدواژه‌ها | ||
| تنوع فیتوشیمیایی؛ خرگوشک (Verbascum L.)؛ ترکیب‌های فنلی؛ فنل کل؛ فلاونوئید کل | ||
| عنوان مقاله [English] | ||
| Evaluation of phenolic compounds and antioxidant activity in leaves of different different Verbascum L. species in West Azerbaijan province | ||
| نویسندگان [English] | ||
| S. Amini1؛ A. Hassani2؛ A. Alirezalu3؛ R. Maleki4 | ||
| 1Ph.D. student, Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 2Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 3Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 4Research Department of Chromatography, Iranian Academic Center for Education, Culture and Research (ACECR), Urmia, Iran | ||
| چکیده [English] | ||
| Mullein (Verbascum L.) is a biennial herbaceous medicinal plant belonging to the Scrophulariaceae family. The flowers of this plant are used to treat respiratory disorders. In this study, the genetic diversity of different mullein species from different regions of West Azerbaijan province was investigated based on some phytochemical markers to domesticate this valuable plant. Phytochemical diversity among different species of mullein was evaluated based on the content of carotenoid, beta-carotene, total phenol, total flavonoid, and antioxidant activity (by FRAP and DPPH methods), and identification of phenolic compounds (gallic acid, caffeic acid, chlorogenic acid, rutin, coumaric acid, quercetin, cinnamic acid, and apigenin) by HPLC in leaf. The results showed that all phytochemical characteristics studied were significantly different among species (P<0.01). The highest content of carotenoid (76.27 mg g-1 DW), beta-carotene (5.88 mg 100g-1 DW), total phenol (32.83 mg GAE g-1 DW), total flavonoid (7.13 mg QE g-1 DW) and antioxidant activity by DPPH method (34.66 µg AA mL-1) was observed in V. sinuatum. The results showed that V. erianthum leaves had the highest content of chlorogenic acid, quercetin, cinnamic acid, and apigenin, while the highest content of gallic acid and caffeic acid was observed in V. saccatum. In general, the findings of this research showed that there was a wide variation of mullein species in West Azerbaijan province. Species V. erianthum and V. saccatum had unique phytochemical properties that can be used to design breeding programs and use them in the pharmaceutical industry. | ||
| کلیدواژه‌ها [English] | ||
| Phytochemical diversity, mullein (Verbascum L.), phenolic compounds, total phenol, total flavonoid | ||
| مراجع | ||
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