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ارزیابی روابط ژنتیکی و فیتوشیمی جمعیت‌های Bunium persicum (Boiss.) B. Fedtsch. در رویشگاه‌های طبیعی یزد و کرمان | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 36، شماره 2 - شماره پیاپی 100، خرداد و تیر 1399، صفحه 305-316 اصل مقاله (289.99 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.127522.2625 | ||
| نویسندگان | ||
| سمیرا حسین جعفری* 1؛ امیر سعادت فر2 | ||
| 1پژوهشگر پسادکتری، دانشکده منابع طبیعی و کویرشناسی، دانشگاه یزد، یزد، ایران | ||
| 2استادیار، گروه گیاهان دارویی، پژوهشکده فناوری تولیدات گیاهی، دانشگاه شهید باهنر کرمان، کرمان، ایران | ||
| چکیده | ||
| Bunium persicum (Boiss.) B. Fedtsch. متعلق به تیره چتریان از مهمترین گیاهان دارویی کشور محسوب می‌شود. در این مطالعه روابط ژنتیکی و فیتوشیمیایی برخی جمعیت‌های این گیاه با نشانگر ISSR و روش GC-MS در رویشگاه‌های یزد و کرمان بررسی شد. به‌منظور مطالعه ژنتیکی با استفاده از 10 آغازگر ISSR، نمونه‌برداری از برگ‌های تازه گیاه انجام گردید. استخراج DNA به روش CTAB انجام شد. برای بررسی فیتوشیمیایی، استخراج اسانس از بذرهای زیره به روش تقطیر با آب و با استفاده از دستگاه کلونجر انجام شد. نتایج حکایت از عملکرد بهتر آغازگر ISSR-13 با تعداد 40 باند، بیشترین میزان PIC (0.39) و شاخص نشانگری (15.6) داشت. تجزیه خوشه‌ای با ضریب تشابه جاکارد و الگوریتم UPGMA چهار جمعیت زیره کرمانی مربوط به دو استان را در 6 گروه قرار داد. در تجزیه به مختصات اصلی 60.56% از واریانس کل توسط سه مؤلفه بیان شد و جمعیت‌ها را به‌طور کامل از یکدیگر جدا نمود. نتایج این آنالیز با تجزیه خوشه‌ای و پراکندگی جغرافیایی نمونه‌ها مطابقت داشت. براساس نتایج GC-MS، به‌ترتیب تعداد 13 و 16 ترکیب در هریک از رویشگاه‌های یزد و کرمان شناسایی شد. ترکیب‌های آلفا-پینن، بتا-پینن، پارا-سیمن، لیمونن، گاما-ترپینن و کومین‌آلدئید به‌عنوان ترکیب‌های مشترک و اصلی بین رویشگاه‌های یزد و کرمان شناسایی شدند. براساس نتایج تجزیه خوشه‌ای پارامترهای فیتوشیمی و قرار گرفتن رویشگاه‌های سیرجان (استان کرمان)، مهریز و اردکان (استان یزد) در یک خوشه و رویشگاه سیرچ در خوشه جداگانه می‌توان نتیجه گرفت که گروه‌بندی براساس ترکیبات اسانس با گروه‌بندی ژنتیکی و فاصله جغرافیایی موجود در رویشگاه‌های طبیعی مطابقت ندارد. | ||
| کلیدواژه‌ها | ||
| زیره کرمانی (Bunium persicum (Boiss.) B. Fedtsch.)؛ ارزیابی ژنتیکی؛ نشانگر ISSR؛ فیتوشیمی؛ آنالیز خوشه‌ای | ||
| عنوان مقاله [English] | ||
| Genetic relationship and phytochemical assessment among populations of Bunium persicum (Boiss.) B. Fedtsch. in the natural habitats of Yazd and Kerman | ||
| نویسندگان [English] | ||
| S. Hossein Jafari1؛ A. Saadatfar2 | ||
| 1Faculty of Natural Resources and Desert Study, Yazd University, Yazd, Iran | ||
| 2Department of Medicinal Plant, Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, Kerman, Iran | ||
| چکیده [English] | ||
| Black caraway, Bunium persicum (Boiss.) B. Fedtsch., belonging to the Apiaceae family, is one of the most important medicinal plants in Iran. In this study, genetic and phytochemical relationships were investigated among some populations of this plant using ISSR markers and GC-MS method in Yazd and Kerman habitats. In order to study genetics using 10 ISSR primers, sampling of the fresh plant leaves was done. DNA was extracted using CTAB method. To investigate phytochemicals, the essential oil was extracted from black caraway seeds using hydrodistillation method and Clevenger apparatus. According to the results, ISSR-13 primer had better performance with 40 bands, the highest amounts of PIC (0.39), and Marker Index (15.6). Cluster analysis using Jacquard's coefficient and UPGMA algorithm divided the four black caraway populations of the two provinces into six groups. Principal coordinates analysis showed that three components explained 60.56% of total variance and completely separated the populations. The results of this analysis were consistent with cluster analysis and geographical distribution of samples. Based on the GC-MS results, 13 and 16 compounds were identified in Yazd and Kerman habitats, respectively. The compounds α-pinene, β-pinene, ρ-cymene, limonene, γ-terpinene, and cuminaldehyde were identified as the same and main compounds between Yazd and Kerman habitats. Based on the results of cluster analysis of phytochemical parameters and location of the habitats Sirjan (Kerman province), Mehriz and Ardekan (Yazd province) in one cluster and Sirch in a separate cluster, it can be concluded that grouping based on essential oil compounds does not correspond with genetic grouping and geographical distance in the natural habitats. | ||
| کلیدواژه‌ها [English] | ||
| Bunium persicum (Boiss.) B. Fedtsch, genetic assessment, ISSR marker, phytochemistry, cluster analysis | ||
| مراجع | ||
|
- Abduganiew, B.E., Abdullaev, U.A., Aripov, K.N., Baser, K.H.C. and Oezek, T., 1997. Composition of the essential oil of Bunium persicum (Boiss.) B. Fedtsch. From Tajikestan. Journal of Essential Oil Resources, 9: 597-598. - Adams, R.P., 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Corporation, Carol Stream, USA, 804p. - Amiri, H. and Biraminia, L., 2014. Phytochemistry evaluation of Ferulago angulata, Bunium persicum and Dorema aucheri from Kerman province (Iran). Eco-phytochemical Journal of Medicinal Plants, 4(1): 1-10. - Araujo, F.S., Pacheco, M.V., Vieira, F.A., Ferrari, C.S., Felix, F.C. and Chagas, K.P.T., 2016. ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth. Indesia (Chile) Volumen, 34(3): 47-52. - Ashraf, J., Malik, W., Iqbal, M.Z., Ali Khan, A., Qayyum, A., Noor, E., Abid, M.A., Naseer Cheema, H.M. and Ahmad, M.Q., 2016. Comparative analysis of genetic diversity among bt cotton genotypes using EST-SSR, ISSR and morphological markers. Journal of Agricultural Science & Technology, 18: 517-531. - Azimzadeh, M., Amiri, R., Assareh, M.H., Bihamta, M.R. and Forootan, M., 2012. Genetic diversity of Iranian Bunium persicum germplasm by morphological markers and essential oil components. Journal of Medicinal Plants Research, 6(7): 1119-1129. - Azimzadeh, M., Amiri, R., Assareh, M.H., Bihamta, M.R. and Forootan, M., 2014. Genetic diversity of Iranian Bunium persicum Boiss. ecotypes using sequencing of the ITS region of nuclear ribosomal DNA. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 22(1): 1-10. - Baghizadeh, A., Mashayekhi, Z. and Ebrahimi, M.A., 2018. Investigation of genetic and phytochemical diversity of some catnip (Nepeta cataria L.) populations by RAPD molecular marker and GC/MS method. Iranian Journal of Medicinal and Aromatic Plants Research, 34(5): 836-848. - Bahador, S., Negari, A.K. and Abbaspoor, M., 2009. The effect of planting depth and corm weight on yield and agronomical characteristics of Bunium persicum (Boiss.) B. Fedtsch. Iranian Journal of Medicinal and Aromatic Plants Research, 25(3): 321-332. - Baldemir, A., Topcu, H., Paksoy, M.Y., Motalebipour, E.Z. and Kafkas, S., 2017. First microsatellite markers for Scaligeria lazica Boiss. (Apiaceae) by next generation sequencing: population structure and genetic diversity analysis. Biotechnology & Biotechnological Equipment, 31(3): 535-543. - Barrandeguy, M.E. and Garcia, M.V., 2014. Quantifying genetic diversity: the starting point for population genetic studies using molecular markers. Journal of Genetics, 93(2): 587-589. - Baser, K.H.C., Oezak, T., Abduganiev, B.E., Abdullaev, U.A. and Aripov, K.N., 1997. Composition of the essential oil of Bunium persicum (Boiss.) B. Fedtsch. from Tajikistan. Journal of Essential Oil Research, 9: 579-589. - Brito, F.A., Nizio, D.A.C., Silva, A.V.C., Diniz, L.E.C., Rabbani, A.R.C., Arrigoni-Blank, M.F., Alvares-Carvalho, S.V., Figueira, G.M., Montanari Junior, I. and Blank, A.F., 2016. Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers. Genetic and Molecular Research, 15(3): 1-10. - Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods- a review. International Journal of Food Microbiology, 94(3): 223-253. - Dehghan Kouhestani, S., Baghzadeh, A., Ranjbar, Gh.A. and Babaiyan Jelodar, N.A., 2009. Investigation of genetic diversity in Persian cumin [Bunium persicum (Boiss.) B. Fedtsch.] germplasm from Kerman province using RAPD molecular markers. Iranian Journal of Medicinal and Aromatic Plants Research, 24(4): 414-427. - Farshadfar, M., Moradzade, N., Farshadfar, E. and Shirvani, H., 2017. Genetic diversity among fennel (Fueniculum vulgare Mill.) accessions using morphological and SCoT markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 25(2): 212-231. - Hadian, J., Nejad Ebrahimi, S., Mirjalili, M., Azizi, A., Ranjbar, H. and Friedt, W., 2011. Chemical and genetic diversity of Zataria multiflora Boiss. accessions growing wild in Iran. Chemistery and Biodiversity, 8(1): 176-188. - Haghiroalsadat, F., Azhdari, M., Oroojalian, F., Omidi, M. and Azimzadeh, M., 2015. The chemical assessment of seed essence of three native medicinal plants of Yazd province (Bunium premium, Cuminum cyminum, Trachyspermum copticum) and the comparison of their antioxidant properties. Journal of Shahid Sadoughi University of Medical Sciences, 6(22): 1592-1603. - Hossein Jafari, S., Sepehry, A., Soltanloo, H. and Karimian, A.A., 2018. Genetic differentiation between bitter and sweet asafetida plants using ISSR markers. Molecular Biology Reports, 46(1): 1069-1078. - Janipour, L., Fahmideh, L. and Fazeli Nasab, B., 2017. Genetic assay of some population of Carum carvi medicinal plant using RAPD and ISSR markers. Plant Environmental Physiology, 48(12): 78-91. - Lopez-Pujol, J., Martinell, M.C., Masso, S., Rovira, A.M., Bosch, M., Molero, J., Simon, J. and Blanche, C., 2013. Conservation genetics of Dichoropetalum schottii (Apiaceae): is the legal protection of edge populations consistent with the genetic data?. Annales Botanici Fennici, 50: 269-283. - Mateescu, R., Zhang, Z., Tsai, K., Phavaphutanon, J., Murphy, K., Acland, G. and Todhunter, R., 2005. Analysis of allele fidelity, polymorphic information content and density of microsatellites in a genome-wide screening for hip dysplasia in a crossbreed pedigree. Journal of Heredity, 96: 847-853. - Modareskia, M., Darvishzadeh, R., Hassani, A. and Kholghi, M., 2012. Molecular diversity within and between Ajowan (Carum copticum L.) populations based on inter simple sequence repeat (ISSR) markers. Journal of Plant Molecular Breeding, 1(1): 51-62. - Moghtader, M., Iraj Mansori, A., Salari, H. and Farahmand, A., 2009. Chemical composition and antimicrobial activity of the essential oil of Bunium persicum Boiss. seed. Iranian Journal of Medicinal and Aromatic Plants Research, 25(1): 20-28. - Naghavi, M.R., Gharayazi, B. and Hosseini Salekdeh, Gh., 2007. Molecular Markers. Tehran University Publication Institute. Tehran, Iran, 340p. - Ng, W.L. and Tan, S.G., 2015. Inter-Simple Sequence Repeat (ISSR) Markers: Are We Doing It Right?. ASM Science Journal, 9(1): 30-39. - Parashar, M., Jakhar, M.L. and Malik, C.P., 2014. A review on biotechnology, genetic diversity in cumin (Cuminum cyminum). International Journal of Life Science & Pharma Research, 4(4): 17-34. - Salami, M., Rahimmalek, M. and Ehtemam, M.H., 2017. Genetic variability of outcross and selfed fennel based on morphological and ISSR markers. Journal of Agricultural Science & Technology, 19: 157-172. - Sefidkon, F., Bahmanzadegan, A., Golipour, M., Mozafarian, V. and Meshkizadeh, S., 2010. Identification and comparison of chemical composition of the essential oils of Bunium cylindricum (Boiss & Hohen.) Drude and Bunium rectangulum Boiss. & Hausskn. Iranian Journal of Medicinal and Aromatic Plants Research, 26(3): 305-316. - Singh, D.K., Tewari, R., Singh, N.K. and Singh, S.S., 2016. Genetic diversity cucumber using inter simple sequence repeats (ISSR). Transcriptomics, 4(1): 1-4. - Smith, W.B., Frye, Ch.T., Veliz, E., Hiebler, Sh., Taylor, R.C. and Hunter, K.L., 2015. Genetic variability of Maryland and West Virginia population of the federally endangered plant Harperella nodosa (Rose) (Apiaceae). Northeastern Naturalist, 22(1): 106-119. - Stevens, M.I., Clarke, A.C., Clarkson, F.M., Goshorn, M. and Gemmill, Ch.E.C., 2015. Are current ecological restoration practices capturing natural levels of genetic diversity? A New Zealand case study using AFLP and ISSR data from mahoe (Melicytus ramiflorus). New Zealand Journal of Ecology, 39(2): 190-197. - Taherkhani, P., Noori, N., Akhondzadeh Basti, A., Gandomi, H. and Alimohammadi, M., 2015. Antimicrobial effects of Kermanian black cumin (Bunium persicum Boiss.) essential oil in Gouda cheese matrix. Journal of Medicinal Plants, 54(2): 76-86. - Torabi-Giglou, M., Panahandeh, J., Mohammadi, S.A., Zaree Nahandi, F., Motallebi Azar, A. and Sliwka, J., 2015. DNA and morphological diversity and relationship analysis of selected cultivated, wild potatoes and some promising hybrids. Journal of Biodiversity and Environmental Sciences (JBES), 6(2): 175-186. - Zabet, M., Rahimi, A., Izanlo, A. and Alizadeh, Z., 2019. Investigation of genetic variation in Cumin (Cuminum cyminum) ecotypes of Khorasan province using RAPD and ISSR markers. Agricultural Biotechnology Journal, 11(1): 75-98. - Zanganeh, K., Fakheri, B., Orooji, F., Afzalifar, A. and Makhdoomi, M.A., 2016. Study of genetic diversity in Fennel (Foeniculum vulgare) ecotypes using ISSR marker. Journal of Medicinal Plants Biotechnology, 4(2): 10-20. | ||
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