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ارزیابی روابط ژنتیکی و فیتوشیمی جمعیت‌های Bunium persicum (Boiss.) B. Fedtsch. در رویشگاه‌های طبیعی یزد و کرمان | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 36، شماره 2 - شماره پیاپی 100، خرداد و تیر 1399، صفحه 305-316 اصل مقاله (289.99 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.127522.2625 | ||
| نویسندگان | ||
| سمیرا حسین جعفری* 1؛ امیر سعادت فر2 | ||
| 1پژوهشگر پسادکتری، دانشکده منابع طبیعی و کویرشناسی، دانشگاه یزد، یزد، ایران | ||
| 2استادیار، گروه گیاهان دارویی، پژوهشکده فناوری تولیدات گیاهی، دانشگاه شهید باهنر کرمان، کرمان، ایران | ||
| چکیده | ||
| Bunium persicum (Boiss.) B. Fedtsch. متعلق به تیره چتریان از مهمترین گیاهان دارویی کشور محسوب می‌شود. در این مطالعه روابط ژنتیکی و فیتوشیمیایی برخی جمعیت‌های این گیاه با نشانگر ISSR و روش GC-MS در رویشگاه‌های یزد و کرمان بررسی شد. به‌منظور مطالعه ژنتیکی با استفاده از 10 آغازگر ISSR، نمونه‌برداری از برگ‌های تازه گیاه انجام گردید. استخراج DNA به روش CTAB انجام شد. برای بررسی فیتوشیمیایی، استخراج اسانس از بذرهای زیره به روش تقطیر با آب و با استفاده از دستگاه کلونجر انجام شد. نتایج حکایت از عملکرد بهتر آغازگر ISSR-13 با تعداد 40 باند، بیشترین میزان PIC (0.39) و شاخص نشانگری (15.6) داشت. تجزیه خوشه‌ای با ضریب تشابه جاکارد و الگوریتم UPGMA چهار جمعیت زیره کرمانی مربوط به دو استان را در 6 گروه قرار داد. در تجزیه به مختصات اصلی 60.56% از واریانس کل توسط سه مؤلفه بیان شد و جمعیت‌ها را به‌طور کامل از یکدیگر جدا نمود. نتایج این آنالیز با تجزیه خوشه‌ای و پراکندگی جغرافیایی نمونه‌ها مطابقت داشت. براساس نتایج GC-MS، به‌ترتیب تعداد 13 و 16 ترکیب در هریک از رویشگاه‌های یزد و کرمان شناسایی شد. ترکیب‌های آلفا-پینن، بتا-پینن، پارا-سیمن، لیمونن، گاما-ترپینن و کومین‌آلدئید به‌عنوان ترکیب‌های مشترک و اصلی بین رویشگاه‌های یزد و کرمان شناسایی شدند. براساس نتایج تجزیه خوشه‌ای پارامترهای فیتوشیمی و قرار گرفتن رویشگاه‌های سیرجان (استان کرمان)، مهریز و اردکان (استان یزد) در یک خوشه و رویشگاه سیرچ در خوشه جداگانه می‌توان نتیجه گرفت که گروه‌بندی براساس ترکیبات اسانس با گروه‌بندی ژنتیکی و فاصله جغرافیایی موجود در رویشگاه‌های طبیعی مطابقت ندارد. | ||
| کلیدواژه‌ها | ||
| زیره کرمانی (Bunium persicum (Boiss.) B. Fedtsch.)؛ ارزیابی ژنتیکی؛ نشانگر ISSR؛ فیتوشیمی؛ آنالیز خوشه‌ای | ||
| عنوان مقاله [English] | ||
| Genetic relationship and phytochemical assessment among populations of Bunium persicum (Boiss.) B. Fedtsch. in the natural habitats of Yazd and Kerman | ||
| نویسندگان [English] | ||
| S. Hossein Jafari1؛ A. Saadatfar2 | ||
| 1Faculty of Natural Resources and Desert Study, Yazd University, Yazd, Iran | ||
| 2Department of Medicinal Plant, Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, Kerman, Iran | ||
| چکیده [English] | ||
| Black caraway, Bunium persicum (Boiss.) B. Fedtsch., belonging to the Apiaceae family, is one of the most important medicinal plants in Iran. In this study, genetic and phytochemical relationships were investigated among some populations of this plant using ISSR markers and GC-MS method in Yazd and Kerman habitats. In order to study genetics using 10 ISSR primers, sampling of the fresh plant leaves was done. DNA was extracted using CTAB method. To investigate phytochemicals, the essential oil was extracted from black caraway seeds using hydrodistillation method and Clevenger apparatus. According to the results, ISSR-13 primer had better performance with 40 bands, the highest amounts of PIC (0.39), and Marker Index (15.6). Cluster analysis using Jacquard's coefficient and UPGMA algorithm divided the four black caraway populations of the two provinces into six groups. Principal coordinates analysis showed that three components explained 60.56% of total variance and completely separated the populations. The results of this analysis were consistent with cluster analysis and geographical distribution of samples. Based on the GC-MS results, 13 and 16 compounds were identified in Yazd and Kerman habitats, respectively. The compounds α-pinene, β-pinene, ρ-cymene, limonene, γ-terpinene, and cuminaldehyde were identified as the same and main compounds between Yazd and Kerman habitats. Based on the results of cluster analysis of phytochemical parameters and location of the habitats Sirjan (Kerman province), Mehriz and Ardekan (Yazd province) in one cluster and Sirch in a separate cluster, it can be concluded that grouping based on essential oil compounds does not correspond with genetic grouping and geographical distance in the natural habitats. | ||
| کلیدواژه‌ها [English] | ||
| Bunium persicum (Boiss.) B. Fedtsch, genetic assessment, ISSR marker, phytochemistry, cluster analysis | ||
| مراجع | ||
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