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تأثیر سویه‌های مختلف باکتری سودوموناس فلورسنس بر فاکتورهای مورفولوژیک، بیوشیمیایی و آنزیم‌های آنتی‌اکسیدان گیاه شوید (Anethum graveolens L.) تحت تنش سرب | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 5، دوره 36، شماره 2 - شماره پیاپی 100، خرداد و تیر 1399، صفحه 227-244 اصل مقاله (841.28 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.128076.2656 | ||
| نویسندگان | ||
| اکرم رهبری1؛ حمیده فاطمی* 2؛ بهروز اسماعیل پور3 | ||
| 1دانش‌آموخته کارشناسی ارشد، گروه باغبانی، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 2دانشجوی دکتری، گروه باغبانی، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 3دانشیار، گروه علوم باغبانی، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| چکیده | ||
| اخیراً روش‌های مختلفی برای کاهش اثرهای مخرب آلاینده‌ها مورد استفاده قرار می‌گیرد که کاربرد کودهای زیستی با اثرهای مثبت آنها بر خصوصیات خاک و محیط‌زیست می‌توانند جایگزینی مناسب برای بسیاری از این روش‌ها باشند. به‌منظور بررسی اثر دو سویه باکتری سودوموناس فلورسنس بر شاخص‌های رشد، فیزیولوژیک و بیوشیمیایی گیاه شوید (Anethum graveolens L.) در شرایط تنش سرب، یک آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در دانشگاه محقق اردبیلی در سال 1394 انجام شد. تیمارهای آزمایش شامل تنش سرب در دو سطح (صفر و ppm600) و تیمار تلقیح با باکتری در سه سطح (تلقیح با سویه‌های 150 و 159 سودوموناس فلورسنس به‌صورت جداگانه و بدون تلقیح یا شاهد) بود. نتایج حاصل از این مطالعه نشان داد که صفات رشدی تعداد برگ، تعداد ساقه، ارتفاع بوته، وزن تر و خشک اندام هوایی و وزن تر و خشک ریشه به‌طور معنی‌داری تحت تأثیر آلودگی سرب قرار گرفت و صفت وزن تر و خشک بوته، تعداد برگ و ارتفاع گیاه به‌ترتیب 22، 31، 19 و 9 درصد کاهش یافتند. محتوی پرولین و کربوهیدرات گیاه شوید تحت تأثیر حضور سرب افزایش معنی‌داری نشان داد، همچنین تلقیح با باکتری نیز محتوی پرولین و کربوهیدرات گیاه را افزایش داد که میزان این افزایش در تلقیح با سویه 150 چشمگیرتر بود. فعالیت آنزیم‌های آنتی‌اکسیدانی به‌صورت معنی‌دار تحت تأثیر سرب و باکتری قرار گرفت و حضور سرب به‌ترتیب موجب افزایش و کاهش فعالیت آنزیم پراکسیداز و کاتالاز شد، اما تلقیح با باکتری سودوموناس به‌ویژه سویه 150 سبب افزایش معنی‌دار فعالیت هر دو آنزیم شد. علاوه‌براین باکتری‌ها توانستند میزان سرب گیاه شوید را کاهش دهند. به‌طور کلی نتایج این پژوهش نشان داد که تلقیح با باکتری سودوموناس با افزایش رشد و بهبود فعالیت‌های آنزیمی در کاهش اثرهای تنش سرب بر گیاه شوید کارآمد بودند. | ||
| کلیدواژه‌ها | ||
| پرولین؛ عناصر سنگین؛ کودهای زیستی؛ وزن خشک | ||
| عنوان مقاله [English] | ||
| Effects of different strains of bacterium Pseudomonas fluorescens on morphological and biochemical factors and antioxidant enzymes activities of Dill (Anethum graveolens L.) under Pb stress | ||
| نویسندگان [English] | ||
| A. Rahbary1؛ H. Fatemi2؛ B. Esmailpour3 | ||
| 1M.Sc. student, Department of Horticultural Science, Mohaghegh Ardabili University, Ardebil, Iran | ||
| 2Ph.D. student, Department of Horticultural Science, Mohaghegh Ardabili University, Ardebil, Iran | ||
| 3Department of Horticultural Science, Mohaghegh Ardabili University, University, Ardebil, Iran | ||
| چکیده [English] | ||
| Recently, various methods have been used to alleviate the detrimental effects of pollutants. The application of biofertilizers with respect to their positive impacts on soil characteristics and environment can be served as a suitable alternative to many of these methods. In order to investigate the two-way effect of bacterium Pseudomonas fluorescens on plant growth, physiological and biochemical characteristics of dill (Anethum graveolens L.) under Pb stress conditions, a factorial experiment based on a completely randomized design with three replications was conducted at Mohaghegh Ardabili University. Experimental treatments included Pb stress at two levels (0 and 600 ppm) and bacterial inoculation at three levels (inoculation with Pseudomonas fluorescens strains150 and 159 separately and without inoculation (control)). The results of this study showed that the growth characteristics such as leaf number, plant height, and shoot fresh and dry weight were significantly affected by Pb contamination and shoot fresh and dry weight, leaf number and plant height decreased by 22, 31, 19 and 9%, respectively. The content of proline and carbohydrates in dill showed a significant increase under the influence of lead. Bacterial inoculation also increased the content of proline and plant carbohydrates, which was more pronounced in inoculation with strain150. The activity of antioxidant enzymes was significantly influenced by Pb contamination and bacterial inoculation. Pb increased peroxidase but decreased catalase activity, but inoculation with Pseudomonas bacteria, especially strain 150, significantly increased the activity of both enzymes. In addition, the bacteria were able to reduce the amount of Pb in dill. In general, the results of this experiment indicated that Pseudomonas bacterial inoculation was effective in alleviating the effects of Pb stress on dill via growth promotion and improving enzymatic activity. | ||
| کلیدواژه‌ها [English] | ||
| Proline, heavy metal, biofertilizers, dry weight | ||
| مراجع | ||
|
- Adediran, G.A., Ngwenya, B.T., Mosselmans, J.F.W., Heal, K.V. and Harvie, B.A., 2015. Mechanism behind bacteria induced plant growth promotion and Zn accumulation in Brassica juncea. Journal of Hazardious Matereials, 283: 490-499. - Alef, K., 1995. Field methods: 463-490. In: Alef, K. and Nannipieri, P., (Eds.) Methods in Applied Soil Microbiology and Biochemistry. Academic Press, 608p. - Ali, B., Mwamba, T.M., Gill, R.A., Yang, C., Ali, S. and Daud, M.K., 2014. Improvement of element uptake and antioxidative defense in Brassica napus under lead stress by application of hydrogen sulfide. Plant Growth Regulation, 74(3): 261-273. - Aliasgharzad, N., Molaei, A. and Oustan, S., 2011. Pollution induced community tolerance (PICT) of microorganisms in soil incubated with different levels of Pb. World Academy of Science, Engineering and Technology, 5(12): 438-442. - Anderson, T.H. and Domsch, K., 1990. Application of eco-physiological quotients (qCO2 and qD) on microbial biomasses from soils of different cropping histories. Soil Biology and Biochemistry, 22: 251-255. - Arora, K., Sharma, S. and Monti, A., 2016. Bio-remediation of Pb and Cd polluted soils by switch grass: a case study in India. Intenational Journal of Phytoremediation, 18: 704-709. - Ashraf, M.A., Hussain, I., Rasheed, R., Iqbal, M., Riaz, M. and Arif, M.S., 2017. Advances in microbe-assisted reclamation of heavy metal contaminated soils over the last decade: a review. Journal of Environmental ManageMent, 198: 132-143. - Banchio, E., Xie, X., Zhang, H. and Pare, P.W., 2009. Soil bacteria elevate essential oil accumulation and emissions in sweet basil. Journal of Agricultural and Food Chemistry, 57: 653-657. - Bates, L.S., Waldren, R.P. and Teare, I.D., 1973. Rapid determination of free proline for water-stress studies Plant and Soil, 39: 205-207. - Belimove, A.A., Puhalsky, I.V., Safronova, V.I., Shaposhnikov, A.I., Vishnyakova, M.A. and Semenova, E.V., 2015. Role of plant genotype and soil conditions in symbiotic plant-microbe interactions for adaptation of plants to cadmium polluted soils. Water and Air and Soil Pollution, 226(264): 1-15. - Bharwana, S., Ali, S., Farooq, M., Iqbal, N., Abbas, F. and Ahmad, M., 2013. Alleviation of lead toxicity by silicon is related to elevated photosynthesis, antioxidant enzymes suppressed lead uptake and oxidative stress in cotton. Journal of Bioremediation & Biodegradation, 4: 187. - Bianco, C. and Defez, R., 2009. Medicago truncatula improves salt tolerance when nodulated by an indole3-acetic acidoverproducing Sinorhizobium meliloti strain. Journal of Experimental Botany, 60: 3097-3107. - Braud, A., Jezequel, K., Bazot, S. and Lebeau, T., 2009. Enhanced phytoextraction of an agricultural Cr and Pb-contaminated soil by bioaugmentation with siderophore -producing bacteria. Chemosphere, 74: 280-286. - Cencki, S., Cioerci, I.H., Yildiz, M., Oezay, C., Bozdao, A. and Terzi, H., 2010. Lead contamination reduces chlorophyll biosynthesis and genomic template stability in Brassica rapa L. Environmental Exprimental Botany, 67: 467-473. - Chen, J., Zhu, C., Lin, D. and Sun, Z.X., 2007. The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings. Canadian Journal of Plant Science, 87: 49-57. - Demir, S., 2004. Influence of arbuscular mycorrhiza on some physiological growth parameters of pepper. Turkish Journal Biology, 28: 85-90. - Fatemeh, A., Masoud, T., Pejman, A. and Aidin, H., 2014. Effect of plant growth promoting rhizobacteria (PGPRs) and stratification on germination traits of Crataegus pseudoheterophylla Pojark seeds. Scientia Horticulture, 172: 61-67. - Ghnaya, T., Zaier, H., Baioui, R., Sghaier, S., Lucchini, G. and Sacchi, G.A., 2013. Implication of organic acids in the long-distance transport and the accumulation of lead in Sesuvium portulacastrum and Brassica juncea. Chemosphere, 90(4): 1449-1454. - Ghorbanpour, M., Asgari Lajayer, H. and Hadian, J., 2016. Influence of copper and zinc on growth, metal accumulation and chemical composition of essential oils in sweet basil (Ocimum basilicum L.). Journal of Medicinal and Aromatic Plants Research, 3: 132-144. - Gururani, M.A., Upadhyaya, C.P., Baskar, V., Nookaraju, A., Venkatesh, J. and Park, S.W., 2012. Plant growth-promoting rhizobacteria enhance abiotic stress tolerance in Solanum tuberosum through inducing changes in the expression of ROS-scavenging enzymes and improved photosynthetic performance. Journal of Plant Growth Regulator, 32: 245-258. - Han, Q.Q., Lü, X.P., Bai, J.P., Qiao, Y., Paré, P.W., Wang, S.M., Zhang, J.L., Wu, Y.N., Pang, X.P., Xu, W.B. and Wang, Z.L., 2014. Beneficial soil bacterium Bacillus subtilis (GB03) augments salt tolerance of white clover. Frontier Plant Science, 5: 525. - Harish, S., Kavino, M., Kumar, N., Saravana Kumar, D., Soorianathasundaram, K. and Samiyappan, R., 2008. Biohardening with plant growth promoting rhizosphere and endophytic bacteria induces systemic resistance against banana bunchy top virus. Applied Soil Ecology, 39: 187-200. - Hayat, S.H., Hayat, Q., Alyemeni, M.N., Shafi Wani, A., Pichtel, J. and Aqil Ahmad, A., 2012. Role of proline under changing environments. Plant and Behavior, 7(11): 1456-1466. - Irigoyen, J.J., Einerich, D.W. and Sánchez‐Díaz, M., 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84: 55-60. - Jiang, M., Liu, S., Li, Y., Li, X., Luo, Z. Song, H. and Chen, Q., 2019. EDTA-facilitated toxic tolerance, absorption and translocation and phytoremediation of lead by dwarf bamboos. Ecotoxicology and Environmental Safety, 170: 502-512. - John, R., Ahmad, P., Gadgil, K. and Sharm, S., 2008. Effect of cadmium and lead on growth, biochemical parameters and uptake in Lemna polyrrhiza L. Plant Soil and Environment, 54(6): 262-270. - Kar, M. and Mishra, D., 1976. Catalase, peroxidase and polyphenolxidase activities during rice leaf senescence. Plant Phsiology, 57: 315-319. - Karlidag, H., Esitken, A., Yildirim, E., Figen Donmez, M. and Turan, M., 2011. Effects of plant growth promoting bacteria on yield, growth, leaf water content, membrane permeability and ionic composition of strawberry under saline conditions. Journal of Plant Nutrition, 34: 34-45. - Khatun, S., Ali, E.J., Hahn, K. and Paek, Y., 2008. Cooper toxicity in withania somnifera: growth and antioxidant enzymes responses of in vitro grown plants. Environmental and Experimental Botany, 64: 279-285. - Khoramdel, S., Koocheki, A., Nassiri Mohalatiand, M. and Gorbani, R., 2008. Effects of biological fertilizers application on growth indices of Nigella sativa L. Iranian Agronomy Research, 6: 285-294. - Kumar, R., Mishra, R.K., Mishra, V., Qidwai, A., Pandey, A., Shukla, S.K. and Dikshit, A., 2016. Detoxification and tolerance of heavy metals in plants: 335-359. In: Ahmad, P., (Ed.). Plant Metal Interaction, Elsevier, 652p. - Laidlaw, M.A., Filippelli, G.M., Sadler, R.C., Gonzales, C.R., Ball, A.S. and Mielke, H.W., 2016. Children’s blood lead seasonality in flint, Michigan (USA), and soil-sourced lead hazard risks. International Journal of Environmental Research and Public Health, 13(4): 358. - Lenart, K. and Wolny-Koadka, B., 2013. The effect of heavy metal concentration and soil pH on the abundance of selected microbial groups within ArcelorMittal Poland steelworks in Cracow. Contamination Toxicology, 90: 85-90. - Li, Y., Niu, W., Wang, J., Liu, L., Zhang, M. and Xu, J., 2016. Effects of artificial soil aeration volume and frequency on soil enzyme activity and microbial abundance when cultivating greenhouse tomato. Soil Science Society American Journal, 80: 1208-1221. -Luo, S., Xu, T., Chen, L., Chen, J., Rao, C. and Xiao, X., 2012. Endophyte-assisted promotion of biomass production and metal-uptake of energy crop sweet sorghum by plant-growth-promoting endophyte Bacillus sp. SLS18. Appllied Microbiology Biotechnology, 93: 1745-1753. - Ma, Y., Prasad, M.N.V., Rajkumar, M. and Freitas, H., 2011. Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils. Biotechnology Advance, 29: 248-258. - Maleki, M., Ghorbanpour, M. and Kariman, K., 2017. Physiological and antioxidative responses of medicinal plants exposed to heavy metals stress. Plant Genetics, 11: 247-254. - Manzoni, S., Schimel, J.P. and Porporato, A., 2012. Responses of soil microbial communities to water stress: Results from a meta-analysis. Ecology, 93: 930-938. - Mesa, J., Mateos-Naranjo, E., Caviedes, M.A., Redondo-Gómez, S., Pajuelo, E. and Rodríguez-Llorente, I.D., 2015. Scouting contaminated estuaries: heavy metal resistant and plant growth promoting rhizobacteria in the native metal rhizoaccumulator Spartina maritima. Marine Pollution Bulletin, 90: 150-159. - Mura, A., Pintus, F., Medda, R., Floris, G., Rinaldi, A.C. and Padiglia, A., 2007. Catalase and antiquitin from Euphorbia characias: Two proteins involved in plant defense. Journal of Biochemistry, 72: 501-508. - Nagananda, G.S., Das, A., Bhattacharya, S. and Kalpana, T., 2010. In vitro studies on the effects of biofertilizers (Azotobacter and Rhizobium) on seed germination and development of Trigonella foenum-graecum L. using a novel glass marble containing liquid medium. International Journal of Botany, - Nikalje, G.C. and Suprasanna, P., 2018. Coping with metal toxicity-cues from halophytes. Frontiers in Plant Science, 9: 777. - Omidbaigi, R., 2000. Apporaches Production and Processing of Medicinal Plant (Vol 3). Mashhad Publications, Astan Quds Razavi, 397p. - Asareh, M.H. and Shariat. A. 2009. Salinity resistance in germination stage and growth stage in some Eucalyptus species. Journal Agriculture Science and Natural Resources, 15(6): 145-157. - Pandey, N., Pathak, G.C., Pandey, D.K. and Pandey, R., 2009. Heavy metals, Co, Ni, Cu, Zn and Cd produce oxidative damage and evoke differential antioxidant responses in spinach. Brazilian Journal of Plant Physiology, 21(2): 103-111. - Piotrowska, A., Bajguz, A., Godlewska-Zyłkiewicz, B. and Zambrzycka, E., 2010. Changes in growth, biochemical components, and antioxidant activity in aquatic plant wolffia arrhiza (Lemnaceae) exposed to cadmium and lead. Archives of Environmental Contamination and Toxicology, 58: 594-604. - Posmyk, M.M., Kontek, R. and Janas, K.M., 2008. Antioxidant Enzymes activity and phenolic compounds content in red cabbage seedlings exposed to copper stress. Ecotoxicology and Environmental Safety, 72(2): 596-602. - Probst, B., Schuler, C. and Joergensen, R.G., 2007. Vineyard soils under organic and conventional managementmicrobial biomass and activity indices and their relation to soil chemical properties. Biology and Fertility of Soils, 44: 443-450. - Reeves, R.D. and Brooks, R.R. 1983. Hyperaccumulation of lead and zinc by two metallophytes from mining areas of central Europe. Environmental Polluttion, 31: 277-285. - Renault, P., Ben-Sassi, M. and Berard, A., 2013. Improving the MicroResp™ substrate-induced respiration method by a more complete description of CO2 behavior in closed incubation wells. Geoderma, 207: 82-91. - Ritchie, S.W., Nguyen, H.T. and Holaday, A.S., 1990. Leaf water content and gas-exchange parameters of two wheat genotypes differing in drought resistance. Crop Science, 30: 105-111. - Saravanakumar, D., Kavino, M., Raguchander, T., Subbian, P. and Samiyappan, R., 2010. Plant growth promoting bacteria enhance water stress resistance in green gram plants. Plant Physiology, 33: 203-209. - Shi, W.Y., Du, S., Morina, J.C., Guan, J.H., Wang, K.B., Ma, M.G., Yamanaka, N. and Tateno, R., 2017. Physical and biogeochemical controls on soil respiration along a topographical gradient in a semiarid forestary and. Agricultural Meteorology, 247: 1-11. - Souri, M.K., Alipanahi, N. and Tohidloo, G., 2016. Heavy metal content of some leafy egetable crops grown with waste water in southern suburb of Tehran-Iran. Vegetable Science, 43: 156-162. - Tangahu, B.V., Sheikh Abdullah, S.R., Basri, H., Idris, M., Anuar, N. and Mukhlisin, M., 2011. A review on heavy metals (As, Pb, and Hg) uptake by plants through phytoremediation. International Journal Chemistry Enginering, 2011: 1-31. - TayebRezvani, H., Moradi, P. and Soltani, F., 2013. The effect of nitrogen fixation and phosphorus solvent bacteria on growth physiology and vitamin C content of Capsicum annum L. Iranian Journal of Plant Physiology, 3(2): 673-682. - Turan, M., Güllüce, M., Çakmak, R. and Şahin, F., 2013. Effect of plant growth-promoting rhizobacteria strain on freezing injury and antioxidant enzyme activity of wheat and barley. Journal of Plant Nutrition, 36: 731-748. - Ullah, A., Mushtaq, H., Ali, H., Munis, M.F.H., Javed, M.T. and Chaudhary, H.J., 2015. Diazotrophs-assisted phytoremediation of heavy metals: a novel approach. Environmental Scioiety Pollution Reserch, 22: 2505-2514. - Wani, P.A., Khan, M.S. and Zaidi, A., 2008. Chromium reducing and plant growth promoting Mesorhizobium improves chickpea growth in chromium amended soil. Biotechnology Letters, 30: 159-163. - Yu, C.L., Hui, D., Deng, Q., Dzantor, E.K., Fay, P.A., Shen, W. and Luo, Y., 2017. Responses of switchgrass soil respiration and its components to precipitation gradient in a mesocosm study. Plant and Soil, 420: 105-117. - Zhang, H.H., Tang, M. and Zheng, C., 2010. Effect of inoculation with AM fungi on lead uptake, translocation and stress alleviation of Zea mays L. seedlings planting in soil with increasing lead concentrations. European Journal of Soil Biology, 46: 306-311. | ||
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