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اثر تنش خشکی بر محتوای فیتواسترول‌ها و ژن‌های کلیدی درگیر در مسیر سنتز بتاسیتوسترول در گیاه دارویی کدوی تخم‌کاغذی (Cucurbita pepo L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 4، دوره 36، شماره 2 - شماره پیاپی 100، خرداد و تیر 1399، صفحه 209-226 اصل مقاله (608.07 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.128207.2658 | ||
| نویسندگان | ||
| محمد زینالی* 1؛ بهرام ملکی زنجانی2؛ پرویز مرادی3؛ فرید شکاری2؛ سید محسن نیازخانی4 | ||
| 1دانش‌آموخته دکترا، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه زنجان، زنجان، ایران | ||
| 2دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه زنجان، زنجان، ایران | ||
| 3استادیار، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان زنجان، سازمان تحقیقات، آموزش و ترویح کشاورزی، زنجان، ایران | ||
| 4دانش‌آموخته دکترا، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه ارومیه، ارومیه، ایران | ||
| چکیده | ||
| تنش‌های زیستی ازجمله خشکی بر میزان تولید متابولیت‌های ثانویه به‌ویژه استرول‌های گیاهی (فیتواسترول‌ها) اثر می‌گذارد. در بین فیتواسترول‌های موجود در کدوی تخم‌کاغذی، بتاسیتوسترول از مهمترین مواد مؤثره در آن است که خواص دارویی زیادی نیز دارد. در این مطالعه، اثر پنج سطح تنش خشکی بر روی میزان تولید روغن بذر و فیتواسترول‌های موجود در آن در سه ژنوتیپ و رقم استریاکای کدوی تخم‌کاغذی (Cucurbita pepo L.) مطالعه شد. تجزیه فیتواسترول‌ها با استفاده از GC/MS نشان داد که افزایش میزان تنش خشکی اثر منفی بر میزان تولید روغن حاصل از بذر رسیده کدوی تخم‌کاغذی داشت ولی فیتواسترول‌های تشکیل‌دهنده روغن به‌ویژه بتاسیتوسترول افزایش یافتند و این افزایش در رقم استریاکا (Styriaca) از بقیه ژنوتیپ‌ها بیشتر بود. برای تأیید این نتایج، 30-15 روز بعد از گلدهی دانه‌های آن برداشت و میزان بیان ژن‌های SQS، PP2A، SMT2، ERG26 و Cycloartenol synthase درگیر در مسیر سنتز فیتواسترول‌ها بررسی شد. نتایج مشخص کرد که در بین سطوح مختلف تنش خشکی و ژن‌های مورد مطالعه، در سطح دیم‌کاری ژن‌ها بالاترین میزان بیان را داشته و در آن بازه زمانی، عمده فعالیت مسیر سنتز استروئیدها در کدوی تخم‌کاغذی مسیر سنتز بتاسیتوسترول و استیگماسترول است. | ||
| کلیدواژه‌ها | ||
| بتاسیتوسترول؛ کدوی تخم‌کاغذی (Cucurbita pepo L.)؛ تنش خشکی؛ بیان ژن؛ فیتواسترول؛ رقم استریاکا | ||
| عنوان مقاله [English] | ||
| Effects of drought stress on phytosterols content and expression of key genes involved in Beta sitosterol biosysthesis pathway in medicinal plant pumpkin (Cucurbita pepo L.) | ||
| نویسندگان [English] | ||
| M. Zeynali1؛ B. Maleki Zanjani2؛ P. Moradi3؛ F. Shekari2؛ S.M. Niazkhani4 | ||
| 1Department of Agronomy and Plant Breeding, Faculty of Agriculture, Zanjan University, Zanjan, Iran | ||
| 2Department of Agronomy and Plant Breeding, Faculty of Agriculture, Zanjan University, Zanjan, Iran | ||
| 3Natural Resources Research Division, Agricultural and Natural Resources Research Center of Zanjan, Zanjan Iran | ||
| 4Department of Agronomy and Plant Breeding, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| چکیده [English] | ||
| Biological stresses such as drought affect the production of secondary metabolites, especially plant sterols (phytosterols). Among the phytoestrols found in pumpkin seeds, betasitosterol is one of the most important components which also have many medicinal properties. In this study, the effect of five levels of drought stress on seed oil production and its phytosterols was studied in three genotypes and Styriaca variety of pumpkin (Cucurbita pepo L.). Analysis of phytosterols using GC/MS showed that increasing drought stress had a negative effect on oil production from pumpkin ripe seeds, but oil phytosterols, especially betasitosterol, increased and this increase was higher in Styriaca than other genotypes. To confirm these results, the seeds were harvested 15-30 days after flowering and the expression of SQS, PP2A, SMT2, ERG26 and Cycloartenol synthase genes involved in the phytosterol biosynthesis pathway was investigated. The results indicated that, among the different levels of drought stress and genes studied, highest expression level was observed at the rainfed level and at that time, betasitosterol and stigmasterol pathway had the main activity in steroid biosynthesis pathway in pumpkin. | ||
| کلیدواژه‌ها [English] | ||
| Beta sitosterol, Cucurbita pepo L, drought stress, gene expression, phytosterol, styriaca variety | ||
| مراجع | ||
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