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بررسی تاثیر پیش تیمار بذر با باکتری‌های محرک رشد گیاه بر فعالیت آنزیم‌های آنتی اکسیدانی گیاهچه و شاخص‌های جوانه‌زنی دو رقم گندم نان در شرایط تنش شوری | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 9، شماره 1 - شماره پیاپی 17، خرداد 1399، صفحه 27-44 اصل مقاله (1.61 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2018.122519.1215 | ||
| نویسندگان | ||
| افشین مظفری* 1؛ سجاد فتح اللهی2 | ||
| 1استادیار دانشگاه آزاد اسلامی واحد ایلام، گروه تخصصی زراعت و اصلاح نباتات، ایلام، ایران. | ||
| 2کارشناسی ارشد، دانشگاه آزاد اسلامی واحد ایلام، گروه تخصصی زراعت و اصلاح نباتات، ایلام، ایران. | ||
| چکیده | ||
| به‌منظور بررسی تاثیر بیوپرایمینگ بذر گندم با رایزوباکتری‌های محرک رشد گیاه (PGPR) بر فعالیت آنزیم‌های آنتی اکسیدانتی گیاهچه و شاخص‌های جوانه‌زنی دو رقم گندم نان تحت شرایط تنش شوری، آزمایشی در سال 1396 در آزمایشگاه دانشگاه آزاد اسلامی واحد ایلام به‌صورت فاکتوریل بر پایه طرح کاملاً تصادفی در 3 تکرار اجرا گردید. تنش شوری شامل: صفر (شاهد)، 50، 100 و 200 میلی‌مولار نمک کلرید سدیم، باکتری‌های PGPR شامل: عدم تلقیح بذر با PGPR (شاهد) و تلقیح بذر با PGPR و ارقام گندم شامل: تجن و افق بود. جنس و گونه‌های باکتری‌های PGPR مورد استفاده در این پژوهش شامل chroococcum Azotobacter، Azospirillum berasilense وpotida Pseudomonas بود که از موسسه تحقیقات خاک و آب کشور تهیه شد. صفات آزمایشی شامل فعالیت آنزیم‌های سوپراکسید دیسموتاز (SOD)، کاتالاز (CAT) و گلوتاتیون پرواکسیداز (GPX)، وزن خشک ریشه‌چه، ساقه‌چه و گیاهچه، درصد جوانه‌زنی، سرعت جوانه‌زنی و شاخص بنیه بذر بود. اثر ساده عوامل آزمایش بر تمام صفات بسیار معنی‌دار (01/0≥P) شد. نتایج آزمایش نشان داد که رقم افق نسبت به تجن از نظر شاخص‌های جوانه‌زنی، وزن خشک ریشه‌چه، ساقه‌چه و گیاهچه و فعالیت آنزیم‌های آنتی اکسیدانتی برتر بود. با افزایش شدت تنش شوری همه صفات روند کاهشی داشتند. تیمار پرایم بذر با PGPR در مقایسه با تیمار عدم مصرف باکتری‌های PGPR باعث بهبود صفات آزمایش شد. به‌طورکلی، نتایج آزمایش نشان داد، پرایمینگ بذور گندم با باکتری‌های PGPR در مقایسه با تیمار شاهد (عدم پرایمینگ بذر) از طریق بهبود شاخص‌های جوانه‌زنی و مقدار فعالیت آنزیم‌های آنتی اکسیدانتی گیاهچه تا حدودی آثار مخرب تنش شوری را کاهش داد. | ||
| کلیدواژه‌ها | ||
| باکتری‌های PGPR؛ شوری؛ آنزیم‌های آنتی اکسیدانتی؛ گندم؛ شاخص جوانه‌زنی | ||
| عنوان مقاله [English] | ||
| Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on Antioxidant Enzymes Activity of Seedling and Germination Indices of Two Wheat Cultivar Under Salt Stress Conditions | ||
| نویسندگان [English] | ||
| Afshin Mozafari1؛ Sajad Fathollahy2 | ||
| 1Assistant Professor, Agronomy and plant breeding department, Ilam branch, Islamic Azad university, Iran. | ||
| 2Masters student, Agronomy and plant breeding department, Ilam branch, Islamic Azad university, Iran | ||
| چکیده [English] | ||
| In order to Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on antioxidant enzymes activity of seedling and Germination Indices of Two Wheat Cultivar under Salt Stress Conditions, an experiment was conducted as a factorial experiment based in a completely randomized design with three replications in a laboratory of Islamic Azad University branch of Ilam, in 2017. Experimental factors included salt stress at four levels: zero (Control), 50, 100 and 200 mM sodium chloride, PGPR bacteria in two levels: non-inoculation with PGPR (control) and seed inoculation with PGPR and wheat cultivars include: Tajan and Ofogh. The genus and species of PGPR bacteria used in this study included Azotobacter chroococcum, Azospirillum berasilense and Pseudomonas potida, which were obtained from the Iranian soil and water research institute. Experimental traits included Activity of superoxide dismutase enzymes (SOD), catalase (CAT) and glutathione peroxidase (GPX), dry weight of radicl, gomul and seedling, germination percentage, germination rate and seed vigor index. The main effect of experimental factors on all traits was highly significant (P≤0.01). The results showed that Ofogh was superior to Tajan cultivar for all germination indices, dry weight of radicl, gomul and seedling, Activity of antioxidant enzymes. With increasing salt stress, all of traits were decreasing. Priming seeds with PGPR bacteria increased the traits studied. In general, priming of wheat seeds with PGPR in comparison with control treatment (non-inoculation) by improving germination indices and Activity of antioxidant enzymes to some extent reduced the harmful effects of salinity stress. | ||
| کلیدواژه‌ها [English] | ||
| PGPR, Salinity, Antioxidant enzymes, Germination indices and Wheat | ||
| مراجع | ||
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