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Extraction and Purification of Anticancer Thymoquinone from Seeds of Nigella sativa by Preparative High-performance Liquid Chromatography | ||
| Journal of Medicinal plants and By-products | ||
| دوره 9، Special، خرداد 2020، صفحه 73-79 اصل مقاله (484.22 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2020.121753 | ||
| نویسندگان | ||
| Zohre Ghanavi1؛ َAli Akbar Velayati* 2؛ Parissa Farnia2؛ Amir Mohammad Naji3؛ Sepideh Kalatehjari1 | ||
| 1Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 3Department of Agronomy and Crop Breeding Faculty of Agriculture, Shahed University, Tehran, Iran | ||
| چکیده | ||
| Thymoquinone (TQ) is a monoterpene ketone which is an important bioactive substance in Nigella sativa L. seed. This important natural compound has many potential medical applications for cardiovascular diseases, diabetes, stroke, neurodegenerative diseases and different types of cancer. To date, no studies have focused on development of a reliable industrial method for purification of TQ from this plant as a major challenge. In this study, an efficient extraction and purification method was developed for preparation of TQ from Nigella sativa L. seeds using preparative high-performance liquid chromatography (HPLC). To this end, a two-step procedure was applied for the first time for extraction of TQ: first, maceration using methyl tert-butyl ether (MTBE) was performed; then, liquid-liquid extraction using methanol successfully removed the majority of the impurities. Next, preparative HPLC was carried out for separation and purification of TQ using a C18 column and the mobile phase of methanol and water containing 0.1% trifluoroacetic acid (TFA).The collected peak from preparative HPLC was analyzed by the analytical HPLC as well as GC-MS instrument. Results of HPLC analysis proved the purity of the collected TQ by 97%, while the results of GC-MS identified the main peak appeared at around 15.6 min as TQ by library searching, and determined its purity by 97% based on peak heights. Overall, this method has a potential for industrialization to prepare purified TQ for medicinal applications. | ||
| کلیدواژهها | ||
| Nigella sativa L؛ Thymoquinone؛ Purification؛ Preparative HPLC | ||
| مراجع | ||
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1. Botnick I, Xue W, Bar E, Ibdah M, Schwartz A, Joel DM, Lev E, Fait A, Lewinsohn E. Distribution of Primary and Specialized Metabolites in Nigella sativaSeeds, a Spice with Vast Traditional and Historical Uses. Molecules. 2012;17:10159-10177.
2. Safaei Z, Azizi M, Maryam Y, Aroiee H, DavarynejadGh. The effect of different irrigation intervals and anti-transpiration compounds on yield and yield components of Black Cumin (Nigella sativa). IJABBR. 2014; 2:326-335.
3. Sarwar A, Latif Z. GC-MS characterisation and antibacterial activity evaluation of Nigella sativa oil against diverse strains of Salmonella. Nat Prod Res. 2015;29:447-451.
4. Mehta BK, Pandit V, Gupta M. New principles from seeds of Nigella sativa. Nat Prod Res. 2009;23:138–148.
5. Majdalawieh AF, Fayyad MW. Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review. Int Immuno Pharmacol. 2015;28:295-304.
6. Xixi L, Haji AA, Xuelei X. A new fatty acid ester from Nigella sativa var. hispidula Boiss showing potent anti-protein tyrosine phosphatase 1B activity. Nat Prod Res. 2017;31:1-5.
7. Khader M, Eckl PM. Thymoquinone: an emerging natural drug with a wide range of medical applications. Iran J Basic Med Sci. 2014;17:950-957.
8.Riyaza A, NazirbSh, Khushtara M, Mishraa A, Jahana Y, Ahmada A. Effect of Nigella sativa L. seed extract on cisplatin-induced delay in gastric emptying in Sprague-Dawley rats. Nat Prod Res. 2017;31:588-592.
9. Al-Bukhari. Division (71) on medicine. In: Sahi Al-Bukhari, editor, The collection of authentic sayings of Prophet Mohammad (peace be upon him). 2nd ed. Turkey: Hilal Yayinlari, Ankara. 1976.
10. Islam MH, Ahmad IZ, Salman MT. Antibacterial activity of Nigella sativa oil seed in various germination phases on clinical bacterial strains isolated from human patients. E3 J Biotech Pharma Res. 2013;4:8-13.
11. Salmani JMM, Asghar S, Lv H, Zhou J. Aqueous Solubility and Degradation Kinetics of the Phytochemical Anticancer Thymoquinone; Probing the Effects of Solvents, pH and Light. Molecules. 2014;19:5925-5939.
12. El-Dakhakhny M. Studies on the chemical constitution of Egyptian Nigella sativa L. seeds II the essential oil. Planta Med. 1963;11:465-470.
13.Kokoska L, Havlik J, Valterova I, Sovova H, Sajfrtova M, Jankovska I. Comparison of Chemical Composition and Antibacterial Activity of Nigella sativa Seed Essential Oils Obtained by Different Extraction Methods. J Food Prot. 2008;71:2475-2480.
14.Nickavar B, Mojab F, Javidnia K, Amoli MAR. Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. Z Naturforsch C. 2003;58:629-631.
15.Ghosheh OA, HoudiAA, Crookscor PA. High performance liquid chromatographic analysis of the pharmacologically active quinones and related compounds in the oil of the black seed (Nigella sativa L). J Pharm Biomed Anal. 1999;19:757-762.
16.Ghanavi Z, Velayati AA, Farnia P, Naji AM, Kalatehjari S. Essential Oil Composition of Nigella sativa Seeds from Different Populations of Iran. J Essent Oil Bear Pl. 2018;21:1652-1659.
17.Schönwitz R, Merk L, Ziegler H. Naturally occurring monoterpenoids in needles of Picea abies (L.) Karst Trees. 1987;1:88-93.
18.Muzika RM, Campbell CL, Hanover JW, Smith AL. Comparison of techniques for extracting volatile compounds from conifer needles. J Chem Ecol. 1990;16:2713- 2722.
19. Voss RH, Rapsomatiotis A. An improved solvent extraction based procedure for the gas chromatographic analysis of resin and fatty acids in pulp mill effluents. J Chromatogr. 1985;346:205-214.
20. Lewinsohn E, Savage TJ, Gijzen M, Croteau R. phytochemical analysis, Simultaneous analysis of monoterpenes and diterpenoids of conifer oleoresin. 1993;4:220-225.
21. Bidlingmeyer BA, Preparative Liquid Chromatography, Elsevier, Amsterdam. 1987;1-104. | ||
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