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Isolation and identification of thermotolerant and thermophilic fungi from soil and compost in Kermanshah province (West of Iran) | ||
| Rostaniha | ||
| مقاله 7، دوره 21، شماره 1 - شماره پیاپی 59، آذر 2020، صفحه 79-95 اصل مقاله (538.45 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/botany.2020.341890.1189 | ||
| نویسندگان | ||
| Zahra Yousofvand1؛ Samad Jamali* 2؛ Hadi Khateri2 | ||
| 1MSc Student of Plant Pathology, Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran | ||
| 2Assistant Prof. of Plant Pathology, Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran | ||
| چکیده | ||
| An investigation was carried out on the occurrence of thermotolerantand thermophilic fungi in 11 soil samples collected from cultivated and natural regions in Kermanshah province (Mahidasht, Harsin, Kerend, Eslamabad-e Gharb, Qasr-e Shirin, Sarpol-e Zahab, Javanrood, Gilan-e Gharb), municipal waste compost and mushroom compost, 2017–19. Fungal isolates were recovered using the soil dilution plate method on potato dextrose agar at 45 and 50 °C. Totally, 24 isolates were obtained that were primarily identified using morphological characters and referring to valid taxonomic keys. DNA extraction was carried out using a Genomic DNA Purification kit. The ITS region (ITS1-5.8S-ITS2) of the ribosomal DNA was amplified using ITS1 and ITS4 primers. Fragments about 500–700 bp were amplified after sequencing deposited in GenBank. Based on morphological characters and sequence data of the ITS rDNA, these fungi were identified as: Aspergillus fumigatus, A. nidulans, A. niger, A. terrus, Melanocarpus albomyces*, Malbranchea cinnamomea*, Thermomyces dupontii*, Th. lanuginosus*, and Thielavia arenaria*. Asterisks indicate species that are new records for the mycobiota of Iran. The abundance of thermophilic fungi in municipal waste compost was higher than soil, and Aspergillus species were the most abundant fungi identified in this study. | ||
| کلیدواژه‌ها | ||
| ITS rDNA؛ morphology؛ Onygenales؛ phylogeny؛ Sordariales | ||
| عنوان مقاله [English] | ||
| جداسازی و شناسایی قارچ‌های متحمل به گرما و گرمادوست از خاک و کمپوست در استان کرمانشاه | ||
| نویسندگان [English] | ||
| زهرا یوسفوند1؛ صمد جمالی2؛ هادی خاطری2 | ||
| 1دانشجوی کارشناسی ارشد بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران | ||
| 2استادیار بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران | ||
| چکیده [English] | ||
| به منظور شناسایی قارچ‌های متحمل به گرما و گرمادوست، طی سال‌های ۹8-1396، نمونه‌برداری از خاک، کمپوست ضایعات شهری و کمپوست قارچ خوراکی در شهرستان‌های استان کرمانشاه (ماهیدشت، هرسین، کرند، اسلام‌آباد، قصر شیرین، سرپل‌ذهاب، جوانرود و گیلانغرب) انجام شد. جداسازی این قارچ‌ها از خاک و کمپوست به روش سری رقت روی محیط‌کشت عصاره سیب‌زمینی-دکستروز-آگار در دمای 45 و 50 درجه سلسیوس انجام شد. در مجموع، ۲۴ جدایه قارچ متحمل به گرما و گرمادوست به دست آمد. شناسایی اولیه جدایه‌ها براساس ویژگی‌های رشدی و ریخت‌شناسی و با استفاده از کلیدهای تاکسونومیکی معتبر صورت گرفت. برای شناسایی مولکولی، دی.ان.ای.، جدایه‌ها با استفاده از کیت خالص‌سازی دی.ان.ای. ژنومی استخراج و ناحیه ITS rDNA (ITS1-5.8S-ITS2) با استفاده از آغازگرهای عمومی ITS1و ITS4تکثیر شد. قطعات ۷۰۰–۵۰۰ جفت بازی به دست آمده از واکنش زنجیره‌ای پلیمراز، خالص‌سازی، توالی‌یابی و ویرایش شده و در بانک ژن ثبت شدند. با استفاده از ابزار جستجوی BLAST، توالی‌های ITS rDNA به دست آمده در این بررسی با سایر آرایه‌های معتبر مستخرج از بانک ژن مقایسه شد. در نهایت نه گونه شامل: Aspergilus fumigatus، A. nidulans، A. niger، A. terrus،Melanocarpus albomyces*، Malbranchea cinnamomea*، Thermomyces dupontii*، Th. lanuginosus* و Thielavia arenaria*شناسایی شد که گونه‌های ستاره‌دار برای فلور قارچی ایران جدید می‌باشند. فراوانی قارچ‌های گرمادوست در کمپوست ضایعات شهری بیش‌تر از خاک بود و گونه‌های آسپرژیلوس فراوان‌ترین قارچ‌های شناسایی شده در این تحقیق بودند. | ||
| کلیدواژه‌ها [English] | ||
| ریخت‌شناسی, نواحی رونویسی شده داخلی, فیلوژنی, Sordariales, Onygenales | ||
| مراجع | ||
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