| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,130 |
| تعداد مقالات | 34,813 |
| تعداد مشاهده مقاله | 50,079,820 |
| تعداد دریافت فایل اصل مقاله | 80,385,021 |
Comparative Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Human Brucellosis | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 76، شماره 2، شهریور 2021، صفحه 203-211 اصل مقاله (291.63 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2020.127122.1371 | ||
| نویسندگان | ||
| L Rahbarnia1؛ S Farajnia* 1، 2، 3؛ B Naghili1؛ N Saeedi4 | ||
| 1Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 4Research Center for Pharmaceutical Nanotechnology, Research and Development Complex, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| چکیده | ||
| Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections. The present study aimed to compare the efficiency of conventional microbiological and serological approaches with nested Polymerase chain reaction (nested PCR) for rapid diagnosis of human brucellosis. A total of 120 subjects with symptoms of brucellosis were included in the study. The sensitivity and specificity of nested PCR for the detection of Brucella bacteria were compared with serological and blood culture methods. Out of 120 patients enrolled, brucellosis was detected in 73 (60.83%) cases based on serological tests with a blood culture confirmation in 8.33% of participants. Based on the obtained results, 55% of cases were positive in serum agglutination test (SAT≥1:160), and Coombs (C-SAT≥1:160) tests. Furthermore, seven negative SAT cases were positive in C-SAT as evidence of chronic brucellosis. The results of the 2-mercaptoethanol (2-ME) ≥ 1:80 test were negative in six SAT-positive cases. Based on nested PCR results, 68.18% and 56.06% SAT positive samples were also detected by blood nested PCR and serum nested PCR, respectively. The sensitivity of blood nested PCR was significantly more than serum nested PCR, SAT≥1:160, and blood culture (p <0.001). Moreover, the specificity of blood and serum nested PCR was obtained at 100%, compared to blood culture and SAT≥ 1:160. In the present study, the nested PCR was able to identify chronic brucellosis in SAT negative patients. As evidenced by the obtained results, the nested PCR showed higher efficiency for rapid diagnosis of human brucellosis, as compared to the blood culture method. Furthermore, the findings pointed to the high performance of nested PCR for rapid diagnosis of both chronic and acute brucellosis. | ||
| کلیدواژهها | ||
| Brucellosis؛ C-SAT؛ 2ME؛ Nested PCR؛ SAT | ||
| عنوان مقاله [English] | ||
| ارزیابی مقایسه ای روش nested PCR برای تشخیص سریع بروسلوز انسانی | ||
| چکیده [English] | ||
| بروسلوز به عنوان یک بیماری زونوتیک با درصد مرگ و میر بالا در صورت عدم درمان می باشد. تشخیص اولیه بروسلوز جهت جلوگیری از بروز عونتهای مزمن ضروری می باشد.هذف از این مطالعه مقایسه کارایی روشهای سرولوژیکی و میکروبی مرسوم با روش مولکولی nested PCR جهت تشخیص سریع بروسلوز انسانی می باشد. مواد و روشها: در این مطالعه 120 نفر با نشانه های اولیه بروسلوز تحت نمونه گیری قرار گرفتند. حساسیت و دقت روش nested PCR با روش کشت خون و روش سرولوژیکی مورد مقایسه قرار گرفت. نتایج: از 120 نفر تحت مطالعه بیماری بروسلوز در 60.83% با روش سرولوژیکی شناسایی شد که تنها 8.33% آنها با روش کشت خون تایید گردید..در مجموع 55% موارد در دو تست وserum agglutination test (SAT≥1:160) Coombs (C-SAT≥1:160) مثبت گزارش شدند. علاوه براین 7 مورد در تست SAT منفی بودند که در تست C-SAT مثبت گزارش شدند که نشاندهنده بروز بروسلوز مزمن بودند. نتایج تست 2-mercaptoethanol (2-ME) ≥ 1:80 تنها در 6 مورد از نمونه های مثبت SAT منفی بود. براساس نتایج nested PCR ، 68.18% نمونه های مثبت SAT به وسیله blood nested PCR مثبت گزارش شدند و 56.06% توسط روش serum nested PCR. حساسیت blood nested PCR به طور قابل توجهی با P بحث:براساس یافته های ما ، nested PCR کارایی بالاتری را نسبت به کشت خون برای تشخیص سریع بروسلوز انسانی دارد.نتایج نشاندهنده کارایی بالای nested PCR جهت شناسایی سریع بروسلوز مزمن و حاد دارد. | ||
| کلیدواژهها [English] | ||
| بروسلوز-SAT, C-SAT, 2ME, Nested PCR | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Al Dahouk, S., Nockler, K., 2011. Implications of laboratory diagnosis on brucellosis therapy. Expert Rev Anti Infect Ther 9, 833-845.
Alikhani, M.Y., Hashemi, S.H., Naseri, Z., Farajnia, S., Peeri-Dogaheh, H., 2012. Diagnosis of Human Brucellosis by Blood Culture (BACTEC) and PCR Method via Whole Blood and Serum. Jundishapur J Microbiol 6, 248-251.
Asaad, A.M., Alqahtani, J.M., 2012. Serological and molecular diagnosis of human brucellosis in Najran, Southwestern Saudi Arabia. J Infect Public Health 5, 189-194.
Dadar, M., Shahali, Y., Wareth, G., 2019. Molecular Diagnosis of Acute and Chronic Brucellosis in Humans. In: Arora, P.K. (Ed.), Microbial Technology for the Welfare of Society, Springer Singapore, Singapore, pp. 223-245.
de Glanville, W.A., Conde-Alvarez, R., Moriyon, I., Njeru, J., Diaz, R., Cook, E.A.J., et al., 2017. Poor performance of the rapid test for human brucellosis in health facilities in Kenya. PLoS Negl Trop Dis 11, e0005508.
DelVecchio, V.G., Kapatral, V., Redkar, R.J., Patra, G., Mujer, C., Los, T., et al., 2002. The genome sequence of the facultative intracellular pathogen Brucella melitensis. Proc Natl Acad Sci U S A 99, 443-448.
Deng, Y., Liu, X., Duan, K., Peng, Q., 2019. Research Progress on Brucellosis. Curr Med Chem 26, 5598-5608.
Dias, M., Dias, E., 2015. Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis. CHRISMED J Health Res 2, 136-139.
Djalalinia, S., Arjmand, R., Gholami, M., Shaker, Y., Saeedi Moghaddam, S., Pourrostami, K., et al., 2019. Frequency and Clinical Manifestations of Pediatric Brucellosis in Iran: A Systematic Review. Int J Pediatr 7, 9037-9047.
Esmaeili, H., 2015. Brucellosis in Islamic republic of Iran. J Med Bacteriol 3, 47-57.
Fanni, F., Shahbaznejad, L., Pourakbari, B., Mahmoudi, S., Mamishi, S., 2013. Clinical manifestations, laboratory findings, and therapeutic regimen in hospitalized children with brucellosis in an Iranian Referral Children Medical Centre. J Health Popul Nutr 31, 218-222.
Gemechu, M.Y., Gill, J.P., Arora, A.K., Ghatak, S., Singh, D.K., 2011. Polymerase chain reaction (PCR) assay for rapid diagnosis and its role in prevention of human brucellosis in Punjab, India. Int J Prev Med 2, 170-177.
Ghatak, S., Muthukumaran, R.B., Nachimuthu, S.K., 2013. A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis. J Biomol Tech 24, 224-231.
Golshani, M., Buozari, S., 2017. A review of Brucellosis in Iran: Epidemiology, Risk Factors, Diagnosis, Control, and Prevention. Iran Biomed J 21, 349-359.
Hanaa, M., Ahmed, O., Elabd, S., Mohammed, M., 2016. Polymerase chain reaction (PCR) technique compared to conventional bacteriological and serological techniques in diagnosis of human brucellosis in Egypt. Afr J Microbiol Res 10, 54-61.
Hasanjani Roushan, M.R., Kazemi, S., Fallah Rostami, F., Ebrahimpour, S., 2014. A Study of Brucella Infection in Humans (Review Article). Crescent J Med Biol Sci 1, -.
Hasanjani Roushan, M.R., Marashi, S.M.A., Moulana, Z., 2016. Polymerase Chain Reaction?Based Assays for the Diagnosis of Active and Relapsed Cases of Human Brucellosis. Am J Trop Med Hyg 95, 1272-1276.
Hassanain, N., Ahmed, W., 2012. Efficacy of Serological Tests in Comparison with PCR for Diagnosis of Brucellosis. World J Medical Sci 7, 243-247.
Hekmatimoghaddam, S., Sadeh, M., Khalili, M.B., Mollaabedin, M., Sazmand, A., 2013. Comparison of PCR, Wright agglutination test and blood culture for diagnosis of brucellosis in suspected patients. Pak J Biol Sci 16, 1589-1592.
Jia, B., Zhang, F., Lu, Y., Zhang, W., Li, J., Zhang, Y., et al., 2017. The clinical features of 590 patients with brucellosis in Xinjiang, China with the emphasis on the treatment of complications. PLoS Negl Trop Dis 11, e0005577.
Kanani, N., Lata, J., Bhanderi, B., Chaitanya, J., Rank, D.N., Purohit, H., 2008. Serological, Bacteriological and Molecular Detection of Brucella Infection in Breeding Bulls. Brucellosis 2008 International Research Conference, Royal Holloway College, University of London, UK.
Keid, L.B., Soares, R.M., Vasconcellos, S.A., Salgado, V.R., Megid, J., Richtzenhain, L.J., 2010. Comparison of a PCR assay in whole blood and serum specimens for canine brucellosis diagnosis. Vet Rec 167, 96-99.
Khosravi, A.D., Abassi, E., Alavi, S., 2005. Isolation of Brucella melitensis and Brucella abortus from brucellosis patients by conventional culture method and polymerase chain reaction technique. Pak J Med Sci 22.
Koroglu, M., Aydemir, O.A., Demiray, T., Erkorkmaz, U., Ozbek, A., Altindis, M., 2016. Comparative evaluation of the Brucella Coombs gel test in laboratory diagnosis of human brucellosis. Biotechnol Biotechnol Equip 30, 970-975.
Kumar, S., Tuteja, U., Batra, H.V., 2007. Generation and characterization of murine monoclonal antibodies to genus-specific 31-kilodalton recombinant cell surface protein of Brucella abortus. Hybridoma (Larchmt) 26, 211-216.
Lucero, N.E., Foglia, L., Ayala, S.M., Gall, D., Nielsen, K., 1999. Competitive enzyme immunoassay for diagnosis of human brucellosis. J Clin Microbiol 37, 3245-3248.
Mangalgi, S., Sajjan, A., 2014. Comparison of three blood culture techniques in the diagnosis of human brucellosis. J Lab Physicians 6, 14-17.
Mangalgi, S., Sajjan, A., Mohite, S., 2012. Seroprevalence of Brucellosis among blood donors of Satara District, Maharashtra. J Krishna Inst Medical Sci Univ 1, 55-60.
Masallat, D., Moemen, D., Eid, M., 2013. PCR versus Serology for Diagnosing of Brucellosis in Pregnant Women: Mansoura University Hospital Experience. Egypt J Med Microbiol 22, 133-138.
Mitka, S., Anetakis, C., Souliou, E., Diza, E., Kansouzidou, A., 2007. Evaluation of different PCR assays for early detection of acute and relapsing brucellosis in humans in comparison with conventional methods. J Clin Microbiol 45, 1211-1218.
Pabuccuoglu, O., Ecemis, T., El, S., Coskun, A., Akcali, S., Sanlidag, T., 2011. Evaluation of serological tests for diagnosis of brucellosis. Jpn J Infect Dis 64, 272-276.
Park, S.H., Lee, Y.H., Chu, H., Hwang, S.D., Hwang, K.J., Choi, H.Y., et al., 2012. Application of the microagglutination test for serologic diagnosis of human brucellosis. Osong Public Health Res Perspect 3, 19-23.
Purwar, S., Metgud, S.C., Mutnal, M.B., Nagamoti, M.B., Patil, C.S., 2016. Utility of Serological Tests in the Era of Molecular Testing for Diagnosis of Human Brucellosis in Endemic Area with Limited Resources. J Clin Diagn Res 10, DC26-29. Rajaii, M., Naghili, B., Pourhassan, A., 2005. Comparison of ELISA and STA tests in diagnosis of Brucellosis. Iran J Clin Infect Dis 1.
Tabibnejad, M., Alikhani, M.Y., Arjomandzadegan, M., Hashemi, S.H., Naseri, Z., 2016. The Optimization of Molecular Detection of Clinical Isolates of Brucella in Blood Cultures by eryD Transcriptase Gene for Confirmation of Culture-Negative Samples. Iran Red Crescent Med J 18, e23879.
Vafaei, I., Ahangarzadeh Rezaee, M., Abdinia, B., Kolahdouzan, K., 2019. Epidemiology, clinical manifestations, laboratory findings and outcome of brucellosis in hospitalized children in Northwest of Iran. J Patient Saf Qual Improv 7, 19-23.
Wang, Y., Wang, Z., Zhang, Y., Bai, L., Zhao, Y., Liu, C., et al., 2014. Polymerase chain reaction–based assays for the diagnosis of human brucellosis. Ann Clin Microbiol Antimicrob 13, 31.
Yazew, T., Bejo, S., Bahaman, A., Omar, A., 2009. Comparison of PCR Assay with Serum and Whole Blood Samples of Experimental Trials for Detection and Differentiation of Brucella melitensis. J Anim Vet Adv 8, 1637-1640.
Zamanian, M., Hashemi Tabar, G.R., Rad, M., Haghparast, A., 2015. Evaluation of different primers for detection of Brucella in human and animal serum samples by using PCR method. Arch Iran Med 18, 44-50.
Zerva, L., Bourantas, K., Mitka, S., Kansouzidou, A., Legakis, N.J., 2001. Serum Is the Preferred Clinical Specimen for Diagnosis of Human Brucellosis by PCR. J Clin Microbiol 39, 1661-1664. | ||
|
آمار تعداد مشاهده مقاله: 2,377 تعداد دریافت فایل اصل مقاله: 3,023 |
||
