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Effect of Carum copticum nano-essence against Saprolegnia and Fusarium, and the Use of Multiplex PCR Assay for the Detection of These Organisms in Rainbow trout Oncorhynchus mykiss | ||
| Archives of Razi Institute | ||
| مقاله 7، دوره 76، شماره 2، شهریور 2021، صفحه 231-241 اصل مقاله (596.24 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2020.342066.1450 | ||
| نویسندگان | ||
| P Tour SavadKouhi1؛ H Ahari* 1؛ A. A Anvar2؛ B Jafari3 | ||
| 1Department of Food Sciences and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| This study aimed to investigate the fungal species isolation and confirmation by the Multiplex PCR method in aquatic fish. Evaluation of the inhibitory effect of nano-essential oils of Carum copticum on isolated fungal species was also conducted in this study. The PCR results showed that 3 out of 5 samples were diagnosed with Fusarium solani, and two of them were positive for Saprolegnia. Moreover, in 0.1% of the females' nanoparticles, one peak appeared that showed a particle with an average diameter of 360 nm, and two nanoparticles showed a peak with a mean diameter of 242 nm. The results of minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) showed that 0.01% nano essential oil had 0.08 and 0.07 mg/ml MIC values against Fusarium solani and Saprolegnia, respectively. Gram/ml was on the growth of Fusarium solani species. The essential oils of female plants had an MIC of 0.07 in 0.1% essential oil and 0.03 mg/ml in 0.01% essential oil in Saprolegnia. Furthermore, in the case of 0.1% nano essential oil, the results showed the MIC values of 0.04 and 0.03 mg/ml against Fusarium solani and Saprolegnia, respectively. The MFC values of 0.1% nano essential oil were 0.1 and 0.07 mg/ml against Fusarium solani and Saprolegnia,respectively. It was not found on Fusarium and Saprolegnia. Overall, the results of this study using PCR for direct detection showed that 70% and 50% of the samples were Fusarium solani and Saprolegnia positive, respectively; therefore, the PCR was an efficient method for the detection of fungi. According to the results of nano-essential oil (0.1%) of females, this nano-essence had a strong inhibitory effect on Fusarium solani and Saprolegnia. | ||
| کلیدواژه‌ها | ||
| Carum copticum؛ Fusarium؛ Multiplex PCR؛ Nano-essence؛ Oncorhynchus mykiss؛ Saprolegnia | ||
| عنوان مقاله [English] | ||
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