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Tour SavadKouhi, P., Ahari, H., Anvar, A. A., Jafari, B.. (1399). Effect of Carum copticum nano essence against Saprolegnia and Fusarium and Multiplex PCR assay for the detection of these organisms in rainbow trout Oncorhynchus mykiss. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2020.342066.1450
P. Tour SavadKouhi; H. Ahari; A. A. Anvar; B. Jafari. "Effect of Carum copticum nano essence against Saprolegnia and Fusarium and Multiplex PCR assay for the detection of these organisms in rainbow trout Oncorhynchus mykiss". سامانه مدیریت نشریات علمی, , , 1399, -. doi: 10.22092/ari.2020.342066.1450
Tour SavadKouhi, P., Ahari, H., Anvar, A. A., Jafari, B.. (1399). 'Effect of Carum copticum nano essence against Saprolegnia and Fusarium and Multiplex PCR assay for the detection of these organisms in rainbow trout Oncorhynchus mykiss', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2020.342066.1450
Tour SavadKouhi, P., Ahari, H., Anvar, A. A., Jafari, B.. Effect of Carum copticum nano essence against Saprolegnia and Fusarium and Multiplex PCR assay for the detection of these organisms in rainbow trout Oncorhynchus mykiss. سامانه مدیریت نشریات علمی, 1399; (): -. doi: 10.22092/ari.2020.342066.1450

Effect of Carum copticum nano essence against Saprolegnia and Fusarium and Multiplex PCR assay for the detection of these organisms in rainbow trout Oncorhynchus mykiss

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 22 اردیبهشت 1399  XML اصل مقاله (673.31 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2020.342066.1450
نویسندگان
P. Tour SavadKouhi1؛ H. Ahari email 1؛ A. A. Anvar2؛ B. Jafari3
1Department of Food Science and Technology, Islamic Azad University, Science and Research Branch, Tehran, Iran
2Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran
3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده
The aim of this study was to investigate the fungal species isolation and confirmation by multiplex PCR method in aquatic fish. Evaluation of the inhibitory effect of nano essential oils of Carum copticum on isolated fungal species. PCR results showed that 3 out of 5 samples were diagnosed with Fusarium solani and two of them were positive for saprolgenia. In 0.1% females' nanoparticles, one peak appeared that showed a particle with an average diameter of 360 nm, and two nanoparticles showed a peak with a mean diameter of 242 nm. The results of MIC and MFC measurements showed that nano essential oil (0.01%) had MIC with 0.08 mg/ml concentrations in nano essential oil (0.1%) with MIC with 0.04 mg concentration. Gram/ml was on the growth of Fusarium solani species. The essential oils of female plants had MIC with concentrations of 0.07 in 0.1% essential oil and 0.03 mg / ml in 0.01% oil in saprolegnia. Based on the results of MFC Nano essential oil (0.1%) on Fusarium in the range of 0.1 mg/ml on Saprolgenia 0.07 mg/ml and essential oil (0.01%) It was not found on Fusarium and Saprolegnia. Overall, the results of this study using PCR for direct detection showed that 70% of samples were Fusarium solani and 50% were Saprolgenia positive, so PCR was an efficient method for detection of fungi. Fusarium solani and Saproligenia were evaluated. According to the results of nano-essential oil (0.1%) of females, it had a strong inhibitory effect on Fusarium solani and Saprolgenia.
کلیدواژه‌ها
Inhibitory effect؛ nano-essence؛ Multiplex PCR؛ rainbow trout (Onchorhynchus mykiss)
عنوان مقاله [English]
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