| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,873 |
| تعداد مقالات | 32,558 |
| تعداد مشاهده مقاله | 42,482,561 |
| تعداد دریافت فایل اصل مقاله | 19,247,531 |
Assessment of Mouse Ileal loop Protection against Clinically Isolated Vibrio cholerae Outer Membrane Vesicles as a Vaccine Candidate | ||
| Archives of Razi Institute | ||
| مقاله 5، دوره 75، شماره 4، اسفند 2020، صفحه 451-461 اصل مقاله (1.02 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.126909.1365 | ||
| نویسندگان | ||
| M. Sedaghat1؛ S. D. Siadat* 2، 3؛ F. Shahcheraghi1؛ E. Mirabzadeh Ardakani4؛ M. Keramati5؛ F. Vaziri2، 3؛ S. A. Nojoumi2، 3 | ||
| 1Department of Bacteriology, Pasteur Institute of Tehran, Iran | ||
| 2Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran | ||
| 3Mycobacteriology & Pulmonary Research Department, Pasteur Institute of Iran, Tehran, Iran | ||
| 4Department of Biotechnology Research, Pasteur Institute of Tehran, Iran | ||
| 5Department of Pilot of Nano-Biotechnology, Pasteur Institute of Tehran, Iran | ||
| چکیده | ||
| Cholera, a life-threatening disease caused by the Gram-negative bacterium Vibrio cholera, remains a concern in developing countries. The present study investigated the immunogenicity and protective immunity of outer membrane vesicles (OMVs) and combination of OMV and killed whole cells (WC) of a local strain isolated from the last outbreak in Iran in addition to reference and local strains of V. cholerae El Tor O1 in comparison to Dukoral vaccine in mice model. The protein content, morphology, and size of extracted OMVs were evaluated by electrophoresis and microscopic analyses, respectively. The serum titers of total immunoglobulin G (IgG), IgG1, IgG2a, and immunoglobulin A (IgA) in addition to secretory IgA and total IgG in different mice groups were determined by enzyme-linked immunosorbent assay (ELISA). In addition, fluid accumulation (FA) assay regarding the resistance to live strain of V. cholerae in ligated ileal loops was carried out to determine immunogenicity by OMV or combination of OMV and WC in comparison to that reported for Dukoral vaccine. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified OMVs indicated protein profiles within the range of 34-52 kDa. Furthermore, transmission electron microscopy demonstrated the spherical shaped vesicles of 50-200 nm. The results of ELISA showed significant titers of systemic and mucosal immune anti-OMV IgGs in immunized BALB/c mice with different vaccine regimens. Additionally, a notable increase in the FA ratio was demonstrated in this study. The obtained results of the present study revealed that the WC-OMV combination of local strain can induce a high level of antibody response indicating more protection than OMV or WC separately. Moreover, it can be considered an effective immunogen against V. cholerae. | ||
| کلیدواژه‌ها | ||
| ELISA؛ Ileal loop؛ Outer membrane vesicle؛ Vibrio cholerae؛ Vaccine | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Acevedo, R., Fernandez, S., Zayas, C., Acosta, A., Sarmiento, M.E., Ferro, V.A., et al., 2014. Bacterial outer membrane vesicles and vaccine applications. Front Immunol 5, 121.
Ali, M., Lopez, A.L., You, Y.A., Kim, Y.E., Sah, B., Maskery, B., et al., 2012. The global burden of cholera. Bull World Health Organ 90, 209-218A.
Arakawa, T., Chong, D.K., Langridge, W.H., 1998. Efficacy of a food plant-based oral cholera toxin B subunit vaccine. Nat Biotechnol 16, 292-297.
Azizi, M., Azizi, F., 2010. History of Cholera Outbreaks in Iran during the 19(th) and 20(th) Centuries. Middle East J Dig Dis 2, 51-55.
Basu, S., Pickett, M.J., 1969. Reaction of Vibrio cholerae and choleragenic toxin in ileal loop of laboratory animals. J Bacteriol 100, 1142-1143.
Bishop, A.L., Schild, S., Patimalla, B., Klein, B., Camilli, A., 2010. Mucosal immunization with Vibrio cholerae outer membrane vesicles provides maternal protection mediated by antilipopolysaccharide antibodies that inhibit bacterial motility. Infect Immun 78, 4402-4420.
Borde, A., Larsson, A., Holmgren, J., Nygren, E., 2011. Preparation and evaluation of a freeze-dried oral killed cholera vaccine formulation. Eur J Pharm Biopharm 79, 508-518.
Boustanshenas, M., Bakhshi, B., Ghorbani, M., 2013. Investigation into immunological responses against a native recombinant CTB whole-cell Vibrio cholerae vaccine in a rabbit model. J Appl Microbiol 114, 509-515.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254. Chatterjee, D., Chaudhuri, K., 2011. Association of cholera toxin with Vibrio cholerae outer membrane vesicles which are internalized by human intestinal epithelial cells. FEBS Lett 585, 1357-1362.
Chatterjee, D., Chaudhuri, K., 2013. Vibrio cholerae O395 outer membrane vesicles modulate intestinal epithelial cells in a NOD1 protein-dependent manner and induce dendritic cell-mediated Th2/Th17 cell responses. J Biol Chem 288, 4299-4309.
Claassen, I., Meylis, J., van der Ley, P., Peeters, C., Brons, H., Robert, J., et al., 1996. Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine. Vaccine 14, 1001-1008.
Elson, C.O., Ealding, W., Lefkowitz, J., 1984. A lavage technique allowing repeated measurement of IgA antibody in mouse intestinal secretions. J Immunol Methods 67, 101-108.
Harder, T., Koch, J., Wichmann, O., Hellenbrand, W., 2017. Predicted vs observed effectiveness of outer membrane vesicle (OMV) vaccines against meningococcal serogroup B disease: Systematic review. J Infect 75, 81-94.
Holmgren, J., Czerkinsky, C., 2005. Mucosal immunity and vaccines. Nat Med 11, S45-53.
Kim, S.H., Kim, K.S., Lee, S.R., Kim, E., Kim, M.S., Lee, E.Y., et al., 2009. Structural modifications of outer membrane vesicles to refine them as vaccine delivery vehicles. Biochim Biophys Acta 1788, 2150-2159.
Lebens, M., Karlsson, S.L., Kallgard, S., Blomquist, M., Ekman, A., Nygren, E., et al., 2011. Construction of novel vaccine strains of Vibrio cholerae co-expressing the Inaba and Ogawa serotype antigens. Vaccine 29, 7505-7513.
Mountford, A.P., Fisher, A., Wilson, R.A., 1994. The profile of IgG1 and IgG2a antibody responses in mice exposed to Schistosoma mansoni. Parasite Immunol 16, 521-527.
Pourshafie, M.R., Bakhshi, B., Ranjbar, R., Sedaghat, M., Sadeghifard, N., Zaemi Yazdi, J., et al., 2007. Dissemination of a single Vibrio cholerae clone in cholera outbreaks during 2005 in Iran. J Med Microbiol 56, 1615-1619.
Roier, S., Leitner, D.R., Iwashkiw, J., Schild-Prüfert, K., Feldman, M.F., Krohne, G., et al., 2012. Correction:
Intranasal Immunization with Nontypeable Haemophilus influenzae Outer Membrane Vesicles Induces Cross-Protective Immunity in Mice. PLOS ONE 7, 10.1371/annotation/1370dbc4010-f1114-1342fc-aafa 1373efeef1374d3068.
Schild, S., Nelson, E.J., Bishop, A.L., Camilli, A., 2009. Characterization of Vibrio cholerae outer membrane vesicles as a candidate vaccine for cholera. Infect Immun 77, 472-484.
Schild, S., Nelson, E.J., Camilli, A., 2008. Immunization with Vibrio cholerae outer membrane vesicles induces protective immunity in mice. Infect Immun 76, 4554-4563.
Siadat, S.D., Behzadiyannejad, Q., Tabaraie, B., 2007. Evaluation of Serum Bactericidal Activity Specific for Neisseria meningitidis Serogroup A and B: Effect of Immunization with Neisseria meningitidis Serogroup A Polysaccharide and Serogroup B Outer Membrane Vesicle Conjugate as a Bivalent Meningococcus vaccine candidate. Res J Microbial 5, 436- 444.
Sinha, R., Koley, H., Nag, D., Mitra, S., Mukhopadhyay, A.K., Chattopadhyay, B., 2015. Pentavalent outer membrane vesicles of Vibrio cholerae induce adaptive immune response and protective efficacy in both adult and passive suckling mice models. Microbes Infect 17, 215-227.
Vindurampulle, C.J., Attridge, S.R., 2003. Impact of vector priming on the immunogenicity of recombinant Salmonella vaccines. Infect Immun 71, 287-297.
von Seidlein, L., Jiddawi, M., Grais, R.F., Luquero, F., Lucas, M., Deen, J., 2013. The value of and challenges for cholera vaccines in Africa. J Infect Dis 208 Suppl 1, S8-14.
Wang, Z., Lazinski, D.W., Camilli, A., 2017. Immunity Provided by an Outer Membrane Vesicle Cholera Vaccine Is Due to O-Antigen-Specific Antibodies Inhibiting Bacterial Motility. Infect Immun 85, 1-9.
WHO, 2005. Oral cholera vaccine use in complex emergencies, what next? Global task force on cholera control, Geneva. | ||
|
آمار تعداد مشاهده مقاله: 16,395 تعداد دریافت فایل اصل مقاله: 719 |
||
