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Development of Nano-ELISA Method for Serological Diagnosis of Toxoplasmosis in Mice | ||
| Archives of Razi Institute | ||
| مقاله 2، دوره 75، شماره 4، اسفند 2020، صفحه 419-426 اصل مقاله (387.46 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.123028.1236 | ||
| نویسندگان | ||
| A. Khodadadi1؛ R. Madani* 2، 3؛ N. Hoghooghi Rad4؛ N. Atyabi5 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Proteomics, Biochemistry and Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Karaj, Iran | ||
| 3Department of Microbiology, School of Specialized Science, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 4Department of Pathobiology, Faculty of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 5Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Toxoplasmosis is a widespread parasitic disease caused by a protozoan parasite Toxoplasma gondii. Currently, nanotechnology has been used for the diagnosis of many infectious diseases. It could be due to the fact that nanoparticles play an important role in accurate and fast diagnosis. The purpose of this study was to design a Nano-enzyme linked immunosorbent assay (Nano-ELISA) kit using excreted/secreted (E/S) antigens to have higher sensitivity and specificity than those reported for the designed enzyme-linked immunosorbent assay (ELISA) kit for the diagnosis of Toxoplasmosis in mice. Firstly, the serum samples were collected from 15 infected mice with T. gondii and 15 healthy ones. Then, E/S antigens were separated from parasite tachyzoites and used for designing an ELISA kit. In addition, the mice sera were evaluated using the designed ELISA kit. Finally, the serum samples were assessed by Nano-ELISA kits designed with E/S antigen and conjugate of gold nanoparticles. The obtained results of the present study showed that the sensitivity and specificity of the designed ELISA kit were reported as 80% and 86.66%, respectively, that both improved to 93.33% in these sera with the designed Nano-ELISA kit. This finding revealed the significant improvement of sensitivity and specificity using gold nanoparticles in designing the ELISA kit. Furthermore, according to the literature, the use of E/S antigens in designing recognizable ELISA kits has been always highlighted considering the presence of numerous antigens in T. gondii. The results of this study revealed that the use of E/S antigens in the preparation of an ELISA kit was very effective. This is very important, especially in the lower titers of antibody requiring a more accurate diagnosis. On the other hand, the Nano-ELISA method designed with E/S antigens can be more sensitive and specific than ELISA for the diagnosis of Toxoplasmosis and can be the basis for further studies in this regard. | ||
| کلیدواژه‌ها | ||
| Nano-ELISA؛ Toxoplasmosis؛ Mouse | ||
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| مراجع | ||
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Ambrosi, A., Airo, F., Merkoci, A., 2010. Enhanced gold nanoparticle based ELISA for a breast cancer biomarker. Anal Chem 82, 1151-1156.
Childs, J.E., Seegar, W.S., 1986. Epidemiologic observations on infection with Toxoplasma gondii in three species of urban mammals from Baltimore, Maryland, USA. Int J Zoonoses 13, 249-261.
Cox, F., Wakelin, D., 2010. Immunology and Immunopathology of Human Parasitic Infections. Topley & Wilson's Microbiology and Microbial Infections, pp. 422-442.
Dubey, J.P., 2008. Toxoplasmosis. OIE Terrestrial Manual, pp. 1284-1293.
Fuentes, I., Rodriguez, M., Domingo, C.J., del Castillo, F., Juncosa, T., Alvar, J., 1996. Urine sample used for congenital toxoplasmosis diagnosis by PCR. J Clin Microbiol 34, 2368-2371.
Gamble, H.R., Dubey, J.P., Lambillotte, D.N., 2005. Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pig. Vet Parasitol 128, 177-181.
Genchi, G., Polidori, G.A., Zaghini, L., P.Lanfranchi, 1991. Aseppti epidemiologici della toxoplasmosis nell’allevamento intensive del suino. Arch Vet Ital 42, 105-111.
Glor, S.B., Edelhofer, R., Grimm, F., Deplazes, P., Basso, W., 2013. Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep. Parasit Vectors 6, 85.
Habibi, G., Imani, A., Gholami, M., Hablolvarid, M., Behroozikhah, A., Lotfi, M., et al., 2012. Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran. Iran J Parasitol 7, 64-72.
Hassan, M.M., Mansour, S.A., Atta, M., Shalaby, M.M., Seksaka, M.A., Awad, A., 1997. The importance of detecting circulating Toxoplasma antigens in human cases. J Egypt Soc Parasitol 27, 27-34.
Hauck, T.S., Giri, S., Gao, Y., Chan, W.C., 2010. Nanotechnology diagnostics for infectious diseases prevalent in developing countries. Adv Drug Deliv Rev 62, 438-448.
Hughes, J.M., Williams, R.H., Morley, E.K., Cook, D.A., Terry, R.S., Murphy, R.G., et al., 2006. The prevalence of Neospora caninum and co-infection with Toxoplasma gondii by PCR analysis in naturally occurring mammal populations. Parasitology 132, 29-36.
Jia, C.P., Zhong, X.Q., Hua, B., Liu, M.Y., Jing, F.X., Lou, X.H., et al., 2009. Nano-ELISA for highly sensitive protein detection. Biosens Bioelectron 24, 2836-2841.
Koloren, Z., 2013. Sensitive and Cost-Effective Detection of Toxoplasma Gondii in Water Supplies of the Black Sea in Turkey by Loop-Mediated Isothermal Amplification (LAMP). Biotechnol Biotechnol Equip 27, 3543-3546.
Markell, E., John, D., Krotoski, W., 2006. Medical Parasitology, W.B. Saunders Co.
Meganathan, P., Singh, S., Ling, L.Y., Singh, J., Subrayan, V., Nissapatorn, V., 2010. Detection of Toxoplasma gondii DNA by PCR following microwave treatment of serum and whole blood. Southeast Asian J Trop Med Public Health 41, 265-273.
Montoya, J.G., 2002. Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J Infect Dis 185 Suppl 1, 73-82.
Mosallanejad, B., Avizeh, R., Razi Jalali, M.H., Hamidinejat, H., 2012. Seroprevalence of Toxoplasma gondii Among Wild Rats (Rattus rattus) in Ahvaz District, Southwestern Iran. Jundishapur J Microbiol. 5, 332-335.
Nguyen, T.D., de Kesel, M., Bigaignon, G., Hoet, P., Pazzaglia, G., Lammens, M., et al., 1996. Detection of Toxoplasma gondii tachyzoites and bradyzoites in blood, urine, and brains of infected mice. Clin Diagn Lab Immunol 3, 635-639.
Nishikawa, Y., Claveria, F.G., Fujisaki, K., Nagasawa, H., 2002. Studies on serological cross-reaction of Neospora caninum with Toxoplasma gondii and Hammondia heydorni. J Vet Med Sci 64, 161-164.
Shirbazou, S., Delpisheh, A., Mokhetari, R., Tavakoli, G., 2013. Serologic Detection of Anti Toxoplasma gondii Infection in Diabetic Patients. Iran Red Crescent Med J 15, 701-703.
Suzuki, Y., 2002. Host resistance in the brain against Toxoplasma gondii. J Infect Dis 185, 58-65.
Tavassoli, M., Ghorbanzadehghan, M., Esmaeilnejad, B., 2013. Foll Detection of Toxoplasma gondii in sheep and goats blood samples by PCR-RFLP in Urmia. Vet Res Forum 4, 43-47. | ||
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