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Evaluation of Apoptosis Induction by Newcastle Disease Virus LaSota Strain in Human Breast Carcinoma Cells | ||
| Archives of Razi Institute | ||
| مقاله 8، دوره 75، شماره 3، آذر 2020، صفحه 367-376 اصل مقاله (393.52 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.125824.1322 | ||
| نویسندگان | ||
| A. Kalantari1؛ S. Farashi Bonab* 2؛ H. Keyvanfar3؛ P. Mortazavi4 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran | ||
| 3Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 4Department of Pathology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| The innovation of therapeutic modalities with better clinical efficacy is necessary for the treatment of patients with advanced cancers. Newcastle disease virus (NDV), an avian pathogenic virus, is one of the most promising oncolytic viruses that can replicate selectively in human cancer cells. In humans, NDV can cause transient conjunctivitis and mild flu-like symptoms. However, this virus poses no hazard to human health. The elucidation of the mechanisms of cancer cell killing by NDV is helpful for the clinical application of NDV in cancer patients. Regarding this, the present study was performed to evaluate apoptosis induction by NDV LaSota strain vaccine in human breast carcinoma cells. To this end, MCF-7 cells, a human breast adenocarcinoma cell line, were infected with NDV in vitro. Tumor cell cytotoxicity, apoptosis induction, and expression levels of apoptosis-related genes were examined in NDV-infected breast carcinoma cells. Tumor cell cytotoxicity was measured by 3-[4,5-dimethylthiazol-2yl]2,5-diphenyl-tetrazoliumbromide (MTT) assay. The induction of apoptosis was assessed by annexin V/propidium iodide staining. In addition, the expression levels of apoptosis-related genes were evaluated using real-time reverse transcription polymerase chain reaction (PCR) technique. The NDV showed cytotoxic effects on MCF-7 cells and induced apoptosis in the infected carcinoma cells. The gene expression levels of BAX, caspase-9, and caspase-3, but not BAK-1, were increased in NDV-infected cancer cells, compared to the gene expression levels in the non-infected cancer cells. These results suggest that the induction of the intrinsic pathway of apoptosis is one of the mechanisms that can contribute to cancer cell killing by NDV. Additional studies are required to investigate other probable mechanisms involved in NDV-mediated cancer cell killing. | ||
| کلیدواژه‌ها | ||
| Cancer؛ Newcastle disease virus؛ Oncolytic virus؛ Apoptosis؛ Breast adenocarcinoma cell | ||
| عنوان مقاله [English] | ||
| ارزیابی آپوپتوز القاء شده توسط ویروس بیماری نیوکاسل سویه لاسوتا در سلولهای سرطانی پستان انسان | ||
| چکیده [English] | ||
| ابداع روش های درمانی با کارایی بهتر برای درمان بیماران مبتلا به سرطانهای پیشرفته ضروری میباشد. ویروس بیماری نیوکاسل (Newcastle disease virus/NDV) که یک ویروس بیماریزای پرندگان میباشد از جمله ویروسهای انکولیتیک میباشد که میتواند به صورت انتخابی در سلولهای سرطانی انسان تکثیر یابد.NDVدر انسان التهاب ملتحمه موقتی و علایم شبیه به آنفلوانزای خفیف ایجاد می کند. با وجود این، NDV خطری برای سلامتی انسان ایجاد نمیکند. پی بردن به مکانیسمهایی که توسط آنهاNDVسلولهای سرطانی را نابود میکند به کاربرد بالینیNDVدر درمان بیماران سرطانی کمک میکند. هدف از مطالعه حاضر ارزیابی آپوپتوز القاء شده در سلولهای سرطانی پستان انسان توسطNDVبود. سلولهای 7MCF- (رده سلولی سرطان پستان انسان) باNDVدر محیط کشت آلوده شدند. سیتوتوکسی سیتی سلولهای توموری، القاء آپوپتوز و سطح بیان ژنهای مرتبط با آپوپتوز در سلولهای توموری آلوده بهNDV اندازه گیری شد. سیتوتوکسی سیتی ویروس برای سلولهای سرطانی با روش MTT (3- (4،۵-دی متیل 2-تیازول) 2، 5-دی فنیل تترازولیوم بروماید) ارزیابی شد. القاء آپوپتوز با رنگ آمیزی انکسین V/پروپیدیوم یدید و بیان ژنهای مرتبط با آپوپتوز باReal-time RT-PCR ارزیابی شد.NDVنسبت به سلولهای سرطانی پستان اثرات سیتوتوکسیک نشان داد و در سلولهای سرطانی باعث القاء آپوپتوز شد. سطح بیان ژنهای BAX، کاسپاز-9 و کاسپاز-3 در سلولهای سرطانی آلوده بهNDVافزایش یافت ولی بیان ژن ۱-BAK افزایش پیدا نکرد. این نتایج نشان میدهد القاء مسیر داخلی آپوپتوز میتواند یکی از مکانیسمهای تخریب سرطان توسطNDVباشد. مطالعات بیشتری برای بررسی سایر مکانیسمهای احتمالی تخریب سلول سرطانی توسطNDVلازم است. | ||
| کلیدواژه‌ها [English] | ||
| سرطان, ویروس بیماری نیوکاسل, ویروس انکولیتیک, آپوپتوز, سلول سرطانی پستان | ||
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| مراجع | ||
|
Adams, W.R., Prince, A.F., 1959. Cellular changes associated with infection of the Ehrlich ascites tumor with Newcastle disease virus. A NY Acad Sci 81, 89-100.
Alexander, D.J., 2000. Newcastle disease and other avian paramyxoviruses. Rev Sci Tech 19, 443-462.
Bouchet, B.P., Caron de Fromentel, C., Puisieux, A., Galmarini, C.M., 2006. p53 as a target for anti-cancer drug development. Crit Rev Oncol Hematol 58, 190-207.
Cantin, C., Holguera, J., Ferreira, L., Villar, E., Munoz-Barroso, I., 2007. Newcastle disease virus may enter cells by caveolae-mediated endocytosis. J Gen Virol 88, 559-569.
Chiocca, E.A., Rabkin, S.D., 2014. Oncolytic viruses and their application to cancer immunotherapy. Cancer Immunol Res 2, 295-300.
Chittenden, T., Flemington, C., Houghton, A.B., Ebb, R.G., Gallo, G.J., Elangovan, B., et al., 1995. A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein binding functions. EMBO J 14, 5589-5596.
Csatary, L., 1971. Viruses in the Treatment of Cancer. Lancet 298, 825.
Danial, N.N., Korsmeyer, S.J., 2004. Cell Death: Critical Control Points. Cell 116, 205-219.
Degenhardt, K., Sundararajan, R., Lindsten, T., Thompson, C., White, E., 2002. Bax and Bak independently promote cytochrome C release from mitochondria. J Biol Chem 277, 14127-14134.
Elankumaran, S., Rockemann, D., Samal, S.K., 2006. Newcastle disease virus exerts oncolysis by both intrinsic and extrinsic caspase-dependent pathways of cell death. J Virol 80, 7522-7534.
Elmore, S., 2007. Apoptosis: a review of programmed cell death. Toxicol Pathol 35, 495-516.
Fabian, Z., Csatary, C.M., Szeberenyi, J., Csatary, L.K., 2007. p53-independent endoplasmic reticulum stress-mediated cytotoxicity of a Newcastle disease virus strain in tumor cell lines. J Virol 81, 2817-2830.
Farashi-Bonab, S., Khansari, N., 2017a. Virotherapy with Newcastle Disease Virus for Cancer Treatment and its Efficacy in Clinical Trials. MOJ Immunol 5, 00176.
Farashi-Bonab, S., Khansari, N., 2017b. Immunobiology of anticancer virotherapy with Newcastle disease virus in cancer patients. Vaccin Res Open J 1, 45-53.
Fiola, C., Peeters, B., Fournier, P., Arnold, A., Bucur, M., Schirrmacher, V., 2006. Tumor selective replication of Newcastle disease virus: association with defects of tumor cells in antiviral defence. Int J Cancer 119, 328-338.
Gerl, R., Vaux, D.L., 2005. Apoptosis in the development and treatment of cancer. Carcinogenesis 26, 263-270.
Ghrici, M., El Zowalaty, M., Omar, A.R., Ideris, A., 2013a. Induction of apoptosis in MCF-7 cells by the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus Malaysian strain AF2240. Oncol Rep 30, 1035-1044.
Ghrici, M., El Zowalaty, M., Omar, A.R., Ideris, A., 2013b. Newcastle disease virus Malaysian strain AF2240 induces apoptosis in MCF-7 human breast carcinoma cells at an early stage of the virus life cycle. Int J Mol Med 31, 525-532.
Ilkow, C.S., Swift, S.L., Bell, J.C., Diallo, J.S., 2014. From scourge to cure: tumour-selective viral pathogenesis as a new strategy against cancer. PLoS Pathog 10, e1003836.
Kaufman, H.L., Kohlhapp, F.J., Zloza, A., 2016. Oncolytic viruses: a new class of immunotherapy drugs. Nat Rev Drug Discov 14, 642-662.
Kelly, E., Russell, S.J., 2007. History of oncolytic viruses: genesis to genetic engineering. Mol Ther 15, 651-659.
Kumar, R., Tiwari, A.K., Chaturvedi, U., Kumar, G.R., Sahoo, A.P., Rajmani, R.S., et al., 2012. Velogenic newcastle disease virus as an oncolytic virotherapeutics: in vitro characterization. Appl Biochem Biotechnol 167, 2005-2022.
Lam, K.M., Vasconcelos, A.C., Bickford, A.A., 1995. Apoptosis as a cause of death in chicken embryos inoculated with Newcastle disease virus. Microb Pathog 19, 169-174.
Lang, F.F., Conrad, C., Gomez-Manzano, C., Yung, W.K.A., Sawaya, R., Weinberg, J.S., et al., 2018. Phase I Study of DNX-2401 (Delta-24-RGD) Oncolytic Adenovirus: Replication and Immunotherapeutic Effects in Recurrent Malignant Glioma. J Clin Oncol 36, 1419-1427.
Molouki, A., Hsu, Y.T., Jahanshiri, F., Rosli, R., Yusoff, K., 2010. Newcastle disease virus infection promotes Bax redistribution to mitochondria and cell death in HeLa cells. Intervirology 53, 87-94.
Molouki, A., Yusoff, K., 2012. NDV-induced apoptosis in absence of Bax; evidence of involvement of apoptotic proteins upstream of mitochondria. Virol J 9, 179.
Moore, A.E., Diamond, L.C., Mackay, H.H., Sabachewsky, L., 1952. Influence of hemagglutinating viruses on tumor cell suspensions. II. Newcastle disease virus and Ehrlich carcinoma. Proceedings of the Society for Experimental Biology and Medicine. Soc Exp Biol Med 81, 498-501.
Msaouel, P., Opyrchal, M., Dispenzieri, A., Peng, K.W., Federspiel, M.J., Russell, S.J., et al., 2018. Clinical Trials with Oncolytic Measles Virus: Current Status and Future Prospects. Curr Cancer Drug Targets 18, 177-187.
Ozaki, T., Nakagawara, A., 2011. Role of p53 in Cell Death and Human Cancers. Cancers 3, 994-1013.
Prince, A.M., Ginsberg, H.S., 1957. Immunohistochemical studies on the interaction between Ehrlich ascites tumor cells and Newcastle disease virus. J Exp Med 105, 177-188.
Rajmani, R.S., Gandham, R.K., Gupta, S.K., Sahoo, A.P., Singh, P.K., Kumar, R., et al., 2015. HN Protein of Newcastle Disease Virus Induces Apoptosis Through SAPK/JNK Pathway. Appl Biochem Biotechnol 177, 940-956.
Ravindra, P.V., Tiwari, A.K., Ratta, B., Chaturvedi, U., Palia, S.K., Chauhan, R.S., 2009. Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis. Virus Res 141, 13-20.
Ravindra, P.V., Tiwari, A.K., Ratta, B., Chaturvedi, U., Palia, S.K., Subudhi, P.K., et al., 2008a. Induction of apoptosis in Vero cells by Newcastle disease virus requires viral replication, de-novo protein synthesis and caspase activation. Virus Res133, 285-290.
Ravindra, P.V., Tiwari, A.K., Sharma, B., Rajawat, Y.S., Ratta, B., Palia, S., et al., 2008b. HN protein of Newcastle disease virus causes apoptosis in chicken embryo fibroblast cells. Arch Virol 153, 749-754.
Ries, S.J., Brandts, C.H., 2004. Oncolytic viruses for the treatment of cancer: current strategies and clinical trials. Drug Discov Today 9, 759-768.
Russell, L., Peng, K.W., 2018. The emerging role of oncolytic virus therapy against cancer. Chin Clin Oncol 7, 16.
Santry, L.A., McAusland, T.M., Susta, L., Wood, G.A., Major, P.P., Petrik, J.J., et al., 2018. Production and Purification of High-Titer Newcastle Disease Virus for Use in Preclinical Mouse Models of Cancer. Mol Ther Methods Clin 9, 181-191.
Shamas-Din, A., Kale, J., Leber, B., Andrews, D.W., 2013. Mechanisms of action of Bcl-2 family proteins. Cold Spring Harb Perspect Biol 5, a008714.
Shimizu, S., Narita, M., Tsujimoto, Y., 1999. Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC. Nature 399, 483-487.
Wheelock, E.F., Dingle, J.H., 1964. Observations on the Repeated Administration of Viruses to a Patient with Acute Leukemia. A Preliminary Report. N Engl J Med 271, 645-651.
Wong, R.S., 2011. Apoptosis in cancer: from pathogenesis to treatment. Journal of experimental & clinical cancer research : CR 30, 87.
Zhivotovsky, B., Orrenius, S., 2006. Carcinogenesis and apoptosis: paradigms and paradoxes. Carcinogenesis 27, 1939-1945. | ||
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