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Motamed, N., Shoushtari, A., Fallah Mehrabadi, M. H., Tehrani, F.. (1399). Investigation of avian influenza viruses (H9N2-H5NX) in pigeons during highly pathogenic avian influenza outbreaks in, Iran, 2016. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2019.123439.1250
N. Motamed; A. Shoushtari; M. H. Fallah Mehrabadi; F. Tehrani. "Investigation of avian influenza viruses (H9N2-H5NX) in pigeons during highly pathogenic avian influenza outbreaks in, Iran, 2016". سامانه مدیریت نشریات علمی, , , 1399, -. doi: 10.22092/ari.2019.123439.1250
Motamed, N., Shoushtari, A., Fallah Mehrabadi, M. H., Tehrani, F.. (1399). 'Investigation of avian influenza viruses (H9N2-H5NX) in pigeons during highly pathogenic avian influenza outbreaks in, Iran, 2016', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2019.123439.1250
Motamed, N., Shoushtari, A., Fallah Mehrabadi, M. H., Tehrani, F.. Investigation of avian influenza viruses (H9N2-H5NX) in pigeons during highly pathogenic avian influenza outbreaks in, Iran, 2016. سامانه مدیریت نشریات علمی, 1399; (): -. doi: 10.22092/ari.2019.123439.1250

Investigation of avian influenza viruses (H9N2-H5NX) in pigeons during highly pathogenic avian influenza outbreaks in, Iran, 2016

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 16 اردیبهشت 1399  XML اصل مقاله (330.71 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2019.123439.1250
نویسندگان
N. Motamed email orcid 1؛ A. Shoushtari1؛ M. H. Fallah Mehrabadi1؛ F. Tehrani2
1Department of Poultry Diseases Researches, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREO) Karaj-Iran.
2Iran Veterinary Organization, Tahran, Iran
چکیده
Avian influenza (AI) viruses (H9N2 and H5 subtypes) infections in birds cause major concerns around the world. Most of avian species, such as domestic, pet and wild birds, are natural and experimental host of avian influenza viruses. Globally, there are concerns about members of Columbidae family, Pigeons or doves, for their potential role as interspecies bridge in influenza A viruses ecology. Scientific data about it is still conflicted. They may or not potentially transmit viruses between susceptible populations, or alternatively spread viruses among farms during outbreaks. To monitor H5 and H9 influenza viruses infection status in rural, backyard and domestic birds, a yearly active surveillance program was done from September to October, 2016. In December 2016, an outbreak of highly pathogenic avian influenza(HPAI) virus subtype H5N8 at a layer farm in Tehran province was detected. We conducted a study on detecting H9N2 or H5 infections in pigeons within HPAI H5N8 2016 outbreaks and annual national AI surveillance in Iran. Cloacal swabs and tissues samples (trachea, lung, brain, liver, heart, pancreas and cecal tonsil) were collected and examined by Real-Time RT-PCR method and the virus isolation. All swab samples and tissue samples were negative for H5 nor H9N2 viruses. The negative samples in real time RT-PCR after 3 passages showed negative results in HA and molecular tests. In most samples of dead pigeons, after inoculation in embryonated chicken eggs, Newcastle disease virus was isolated.The negative samples in real time RT-PCR after 3 passages showed negative results in HA and molecular tests. In most samples of dead pigeons, after inoculation in embryonated chicken eggs, Newcastle disease virus was isolated
کلیدواژه‌ها
pigeon؛ real time RT-PCR؛ H5 outbreak؛ surveillance
عنوان مقاله [English]
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