| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,829 |
| تعداد مقالات | 32,098 |
| تعداد مشاهده مقاله | 40,864,085 |
| تعداد دریافت فایل اصل مقاله | 18,113,701 |
بررسی شاخص های مهم مرتبط با بیماری ویروسی وای سیب زمینی (Potato virus Y)در مزارع توتون استان گلستان | ||
| آفات و بیماری‌های گیاهی | ||
| مقاله 7، دوره 87، شماره 2 - شماره پیاپی 109، اسفند 1398، صفحه 227-240 اصل مقاله (947.48 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2019.127197.1297 | ||
| نویسندگان | ||
| پریسا شریفی نظام آباد1؛ سعید نصراله نژاد* 2؛ محمدعلی آقاجانی3؛ اکبر دیزجی4؛ احمد ندیمی5 | ||
| 1گروه گیاه پزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 2دانشیار گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ایران | ||
| 3بخش تحقیقات گیاه پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، گرگان. | ||
| 4عضو هیات علمی گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران | ||
| 5گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران؛ | ||
| چکیده | ||
| ویروس وای سیب‌زمینی (Potato virus Y, PVY)، گونۀ تیپ جنس Potyvirus از تیرة Potyviridae، از جمله ویروس‌های مخرب توتون در دنیا می‌باشد. به منظور بررسی وضعیت بیماری ناشی از این ویروس در مزارع توتون استان گلستان، طی فصل زراعی 1396، تعداد 15 مزرعه توتون در سه شهرستان گرگان، علی‌آباد و مینودشت انتخاب شده و در فواصل زمانی 15 روزه مورد بازدید قرار گرفت. در هر مزرعه 50 بوته انتخاب و شاخص‌های میزان وقوع بیماری (DI) و فراوانی دو شته ناقل سبز هلو (Myzus persicae, MP) و قرمز توتون (Myzus persicae ssp. nicotiana, MPN) در آنها ارزیابی گردید. به منظور ردیابی و ارزیابی غلظت نسبی ویروس (OD) از روش DAS-ELISA با استفاده از آنتی‌سرم چند همسانه‌ای اختصاصی PVY استفاده شد. درصد آلودگی PVY در بین ارقام مختلف توتون متفاوت بوده و بیشینه و کمینه درصد آلودگی به تریتب در ارقام PVH03 (6/33 %) و بارلی (2/6 %) ارزیابی شد. بر اساس نتایج حاصل از تحلیل آماری داده‌ها، بین ارقام توتون در تمام شاخص‌های مورد ارزیابی تفاوت معنی‌دار بوده و ارقام باسما و بارلی نسبت به ارقام گرمخانه‌ای (PVH03، NC100 و K326) دارای حساسیت کمتری به PVY بودند. بیشترین جمعیت هر دو شته روی رقم K326 مشاهده گردید. برای شاخص-های DI و OD ارتباط همبستگی با دما غیرمستقیم و رطوبت نسبی و میزان بارندگی مستقیم ارزیابی شد و DI بیشترین میزان همبستگی را با تغییرات بارندگی (r= 0.98 و P= 0.004) داشت. | ||
| کلیدواژه‌ها | ||
| توتون؛ شته سبز هلو؛ PVH03؛ Disease incidence؛ DAS-ELISA | ||
| عنوان مقاله [English] | ||
| Investigating the Potato virus Y main disease indicators in tobacco fields of Golestan province | ||
| نویسندگان [English] | ||
| parisa sharifi-nezamabad1؛ saeed nasrollanejad2؛ Mohammad Ali Aghajani3؛ Akbar Dizadji4؛ Ahmad Nadimi5 | ||
| 1department of plant pathology, faculty of plant production, Gorgan university of Agricultural sciences and Natural resource | ||
| 2Associate Prof, Dept of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources | ||
| 3Department of Plant Protection, Agricultural and Natural Resources Research Center of Golestan province, Gorgan. | ||
| 4Department of Plant Protection, college of Agriculture and Natural Resources, University of Tehran | ||
| 5Department of Plant Protection, Faculty of Plant Production, University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده [English] | ||
| Potato virus Y, is the type member of the genus Potyvirus, family Potyviridae is of the most destructive viruses of tobacco worldwide. In order to detect PVY and its distribution in Golestan province, several surveys were conducted during July to October 2017 at 15 days intervals in tobacco fields of Gorgan, Aliabad and Minoodasht. 50 plant was selected for each farm and various disease indicators and the virus vector (MP and MPN( were evaluated. Double antibody sandwich-ELISA (DAS-ELISA) using PVY-specific polyclonal antibodies was used to estimate changes in the relative concentrations of PVY. The rate of PVY infection was different among tobacco cultivars and the maximum and minimum infection rates were belonged to PVH03 (33.6%) and Barley (6.2%) cultivars respectively. Based on the statistical analysis of data, there was a significant difference for disease indicators and the virus vector on different tobacco cultivars, as Basma and Barley were less susceptible to PVY than Flue-cured cultivars (PVH03, NC100 and K326). Also, the K326 cultivar has the highest Myzus persicae ssp. nicotiana and Myzus persicae populations. There was a direct correlation between DI and OD and relative humidity and rainfall changes so that DI with rainfall changes had the highest Correlation (r = 0.98 and P = 0.004). | ||
| کلیدواژه‌ها [English] | ||
| Tobacco, Disease incidence, DAS-ELISA, Myzus persicae, PVH03 | ||
| مراجع | ||
|
References
AGHAJANI, M. A. 2008. Epidemiologies of Sclerotinia stem rot of Canola in Golestan province. Ph. D. Thesis, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. BARRETT, L. G., P. H. THRALL., J. J. BURDON. and C. C. LINDE, 2008. Life history determines genetic structure and evolutionary potential of host–parasite interactions. Trends in Ecology & Evolution, 23 (12), 678–685. CARDOSO, J. E., A. A. SANTOS, A. G. ROSSETTI and J. C. VIDAL, 2004. Relationship between incidence and severity of cashew gummosis in semiarid north-eastern Brazil. Plant Pathology, 53: 363-367. CAMPBELL, C. L. and L. V. MADDEN, 1990. Introduction to plant disease epidemiology. John Wiley, New York, 532 pp. CHEN, X. M., J. LIU, L. XU, F. JIANG, X. Y. XIE, C. X. ZHU and F. J. WEN, 2010. Inhibiting virus infection by RNA interference of the eight functional genes of the Potato Virus Y genome. Journal of Phytopathology, 158 (12): 776-784. CHIKH-ALI, M., S. M. GRAY, and A. V. KARASEV, 2013. An improved multiplex IC-RT-PCR assay distinguishes nine strains of Potato virus Y. Plant Diseases, 97:1370-1374. CLARK, M. F., and A. N. ADAMS, 1977. Characteristics of the microplate method of Enzyme-linked immunosrobent assay for the detection of plant viruses. Journal of General Virology, 34: 475-483. COOKE, B. M. 2006. Disease assessment and yield loss. Pages 43-80 in: The Epidemiology of Plant Diseases, B. M. COOKE, D. G. JONES and B. KAYE, eds. Springer, Netherlands. CORESTA Guide, 2009. A Scale for Coding Growth Stages in Tobacco Crops in: Growth Stages of Mono- and Dicotyledonous- Plants’, BBCH Monograph, 2nd edition, 2001, (Eds.) U, MEIER, Federal Biological Research Centre for Agriculture and Forestry (nd www.bba.de/veroeff/bbch/bbcheng.pdf ) ENGERING, A., L. HOGERWERF and J. SLINGENBERGH, 2013. Pathogen–host–environment interplay and disease emergence. Emerging Microbes & Infections, 2 (2), e5. HOSSEINZADEH FASHALMI, N., A. MAHDAVI., A. GHOLIZADEH and H. KHATERI, 2012. Study on the compatibility and survival of the Flue-cured tobacco cultivars in Golestan and Mazandaran provinces, The 12th Iranian Congress of Genetics, Tehran, Iran. HOSSEINI, A., SAJJADI, A and MAHDAVI, A. R. 2016. Evaluation the reaction of breeded male sterile air-cured tobacco hybrids to Potato virus Y and cucumber mosaic virus. Proceeding of the 22nd Iranian Plant Protection Congress, College of Agricultural and Natural Resources, University of Tehran, Karaj, IRAN, 17 p. KERLAN, C. 2006. Potato virus Y. Descriptions of plant viruses, no. 414. (http://www.dpvweb.net/ dpv/showdpv.php?dpvno=414) KHATERI, H. 2015. Generation of PVY-resistant transgenic tobacco plants with using RNA-mediated gene silencing. Ph. D. Thesis, Department of Plant Pathology, Faculty of Agricultural and Natural Resources, University of Tehran, Karaj, IRAN. KIRCHNER S. M., L HILTUNEN, T. F. DORING, E. VIRTANEN, J. P. PALOHUHTA and J. P. T. VALKONEN, 2013. Seasonal phenology and species composition of the aphid fauna in a northern crop production area. PLoS One, 8 :e71030 KIRCHNER S. M., L. HILTUNEN, J. SANTALA, T. F. DORING, J. KETOLA, A. KANKAALA, E. VIRTANEN, and J. P. T. VALKONEN, 2014. Comparison of straw mulch, insecticides, mineral oil, and birch extract for control of transmission of Potato virus Y in seed potato crops. Potato Research, 57:59–75. KOVAC, M., A. MULLER., J. D. MILOVANOVIC., M. MILAVEC., P. DUCHTING and M. RAVNIKAR, 2009. Multiple hormone analysis indicates involvement of jasmonate signalling in the early defence of potato to potato virus Y NTN. Biologia Plantarum, 53:195–199. LACROIX, C., L. GLAIS, C. KERLAN, J. L. VERRIER and E. JACQUOT, 2010. Biological characterization of French Potato virus Y (PVY) isolates collected from PVY-susceptible or -resistant tobacco plants possessing the recessive resistance gene va. Plant Pathology Journal, 59 (6): 1133-1143. LACOMME, C., J. PICKUP., A. FOX., L. GLAIS., B. DUPUIS., T. STEINGER., J. L. ROLOT., J. P. T. VALKONEN., K. KRUGER., X. NIE., S. MODIC., N. MEHLE., M. RAVINKAR and M. HULLE, 2017. Transmission and Epidemiology of Potato virus Y. In: Potato virus Y: biodiversity, pathogenicity, epidemiology and management (Eds.) C. LACOMME., L. GLAIS., D. U. BELLSTED., B. DUPUIS., A. V. KARASEV and E. JACQUOT. Published by Springer Nature. pp 141–176. MILAVEC, M., M. RAVNIKAR and M. KOVAC, 2001a. Peroxidases and photosynthetic pigments in susceptible potato infected with Potato virus Y NTN. Plant Physiology and Biochemistry, 39:891–898. MILAVEC, M., M. RAVNIKAR and M. KOVAC, 2001b. Peroxidases in the early response of potato (Solanum tuberosum L. cv. Igor) susceptible to Potato virus Y NTN. Acta Biologica Slovenica, 44:3–11. MILAVEC, M., K. GRUDEN., M. RAVNIKAR and M. KOVAC, 2008. Peroxidases in the early responses of different potato cultivars to infection by Potato virus Y NTN. Plant Pathology, 57:861–869. NANAYAKKARA, U. N., M. SINGH, Y. PELLETIER and X. NIE, 2012. Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada. American Journal of Potato Research, 89: 232–239. PAGAN, I., P. GONZALEZ-JARA., A. MORENO-LETELIER., M. RODELO-URREGO., A. FRAILE., D. PINERO, 2012. Effect of biodiversity changes in disease risk: exploring disease emergence in a plant-virus system. PLOS Pathogens, 8:e1002796. POLHEMUS, N. W, 1980. Interactive statistical graphics: system design criteria with an implementation in APL. Proceedings of the Statistical Computing Section, American Statistical Association, 391-396. POWELL, G, 1991. Cell membrane punctures during epidermal penetrations by aphids: consequences for the transmission of two potyviruses. Annals of Applied Biology, 119 (2): 313-321. ROBERT, Y. and D. BOURDIN, 2001. Aphid transmission of potato viruses. In: Virus and virus-like diseases of potatoes and production of seed-potatoes (Eds.) G. LOEBENSTEIN, P. H. BERGER, A. A. BRUNT and R. H. LAWSON, Doordrecht: Kluwer. pp. 195–225. RUESINK, W. G. and M. E. IRWIN, 1986. Soybean mosaic virus epidemiology: a model and some implications. In: Plant virus epidemics: monitoring, modeling and predicting outbreaks (Eds.) G. MCLEAN., R. GARRETT and W. RUESINK, Academic Press, Sydney, Australia, pp. 295–313. SCHOLTHOF, K. B., S. ADKINS, H. CZOSNEK, P. PALUKAITIS, E. JACQUOT, T. HOHN, B. HOHN, K. SAUNDERS, T. CANDRESSE, P. AHLQUIST, C. Hemenway and G. FOSTER, 2011. Top 10 plant viruses in molecular plant pathology. Molecular Plant Pathology, 12, 938–954. SINGH, R. P., J. P. T. VALKONEN, S. M. GRAY, N. BOONHAM , R. A. C. JONES, C. KERLAN and J. SCHUBERT, 2008. Discussion paper: The naming of Potato virus Y strains infecting potato. Archives of Virology, 153: 1–13. STEINGER, T., H. GILLIAND and T. HEBEISEN, 2014. Epidemiological analysis of risk factors for the spread of potato viruses in Switzerland. Annals of Applied Biology, 164: 200–207. TIAN, Y. P., J. L. LIU, C. L. ZHANG, Y. Y. LIU, B. WANG, X.-D. LI, Z. K. GUO, and J. P. T. VALKONEN, 2011. Genetic diversity of Potato virus Y infecting tobacco crops in China. Phytopathology. 101: 377-387. TSEDALEY, B. 2015. A Review Paper on Potato Virus Y (PVY) Biology, Economic Importance and its Managements. Journal of Biology, Agriculture and Healthcare, 5 (9): 110-126. WOOLHOUSE, M. E. J., S. GOWTAGE-SEQUERIA, 2005. Host range and emerging and reemerging pathogens. Emerging Infectious Diseases, 11, 1842–1847. ZHU, Y. Y., H. FANG., Y. Y. WANG., J. X. FAN and S. S. YANG, 2005. Panicle and canopy moisture in rice cultivar mixtures. Phytopathology, 95: 433–438. TSEDALEY, B. 2015. A Review Paper on Potato Virus Y (PVY) Biology, Economic Importance and its Managements. Journal of Biology, Agriculture and Healthcare, 5 (9): 110-126. WOOLHOUSE, M. E. J., S. GOWTAGE-SEQUERIA, 2005. Host range and emerging and reemerging pathogens. Emerging Infectious Diseases, 11, 1842–1847. ZHU, Y. Y., H. FANG., Y. Y. WANG., J. X. FAN and S. S. YANG, 2005. Panicle and canopy moisture in rice cultivar mixtures. Phytopathology, 95: 433–438.
| ||
|
آمار تعداد مشاهده مقاله: 522 تعداد دریافت فایل اصل مقاله: 328 |
||