| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شماره‌ها | 2,810 |
| تعداد مقالات | 31,895 |
| تعداد مشاهده مقاله | 40,508,880 |
| تعداد دریافت فایل اصل مقاله | 17,861,954 |
تعیین خصوصیات فنوتیپی و تنوع ژنتیکی جدایه‌های Pseudomonas syringae pv. syringae عامل شانکر باکتریایی درختان میوه هسته‌دار در استان‌های خراسان رضوی و شمالی | ||
| آفات و بیماری‌های گیاهی | ||
| مقاله 4، دوره 87، شماره 2 - شماره پیاپی 109، اسفند 1398، صفحه 181-193 اصل مقاله (799.39 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2019.124499.1276 | ||
| نویسندگان | ||
| حمیده احسنی1؛ سعید نصراله نژاد2؛ حشمت اله رحیمیان3؛ جواد محمودی صفا* 4 | ||
| 1گروه گیاه پزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی ومنابع طبیعی گرگان، گرگان، ایران | ||
| 2دانشیار گروه گیاهپزشکی دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 3استاد گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران | ||
| 4گروه گیاه پزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| چکیده | ||
| باکتری Pseudomonas syringae pv. syringae (Pss)عامل یکی از بیماری‌های شایع در باغ‌های درختان میوه هسته‌دار می‌باشد. به منظور شناسایی جدایه‌های عامل بیماری، طی سال‌های 1391-1390، 23 جدایه به‌دست آمده از باغ‌های درختان میوه هسته‌دار واقع در مناطق مختلف استان‌های خراسان رضوی و شمالی از نظر فنوتیپی (فیزیولوژیکی و بیوشیمیایی) و بیماری‌زایی مورد بررسی قرار گرفتند. جدایه‌ها از نظر ویژگی‌های فنوتیپی اختلافات جزئی نشان دادند. همه جدایه‌ها روی هلو و بادام دارای قدرت بیماری‌زایی بالایی بودند و از این نظر مشابه به نظر رسیدند. صحت تشخیص جدایه‌ها در سطح گونه توسط واکنش زنجیره‌ای پلیمراز و با استفاده از آغازگر‌های D21/D22 اختصاصی گونه مورد تایید قرار گرفت. تنوع ژنتیکی جدایه‌ها به دو روشrepetitive element palindromic PCR (rep-PCR) و با استفاده از آغازگر‌های BOXA1R، ERIC1R و ERIC2 و روش Insertion Sequence PCR (IS-PCR) و با استفاده از آغازگر IS50 مورد بررسی قرار گرفت. آنالیز خوشه‌ای نتایج به دست آمده نشان داد که در BOX-PCR با آغازگر BOXA1Rدر سطح تشابه 75 درصد، جدایه‌ها به 7 گروه؛ در ERIC-PCR با آغازگر ERIC1R در سطح تشابه 65 درصد، جدایه‌ها به 7 گروه و با آغازگر ERIC2 در سطح تشابه 56 درصد، جدایه‌ها به 8 گروه؛ در IS-PCR با آغازگرIS50 در سطح تشابه 52 درصد، جدایه‌ها به 11 گروه و در نهایت با ترکیب هر چهار آغازگر در سطح تشابه 54 درصد جدایه‌ها به 7 گروه قابل تفکیک هستند. نتایج بیانگر تنوع ژنتیکی بالا درون جدایه‌های عامل بیماری شانکر باکتریایی درختان میوه هسته‌دار در استان‌های مورد مطالعه است. | ||
| کلیدواژه‌ها | ||
| شانکر باکتریایی؛ تنوع ژنتیکی؛ درختان میوه هسته دار؛ rep-PCR؛ IS-PCR | ||
| عنوان مقاله [English] | ||
| Determination of Phenotypic Properties and Genetic Diversity of Pseudomonas syringae pv. syringae Strains Causing Bacterial Canker in Stone Fruits in Razavi and Northern Khorasan Provinces. | ||
| نویسندگان [English] | ||
| hamideh ahsani1؛ saeed nasrollanejad2؛ heshmat rahimian3؛ javad mahmoudi safa4 | ||
| 1Dept. Plant Pathology,Gorgan University of Agriculture Sciences and Natural Resources, Gorgan, Iran | ||
| 2Associate Professor, Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, | ||
| 3Professor, Dept. Plant Protection, Sari University of Agriculture Sciences and Natural Resources, sari, Iran | ||
| 4Dept. Plant Pathology,Gorgan University of Agriculture Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده [English] | ||
| Pseudomonas syringae pv. syringae (Pss) is the causative agent one of the bacterial common diseases in stone fruit trees fields. In this research, samples were collected from various areas in Razavi and Northern Khorasan provinces during 2011-2012. A total of 23 bacterial isolates were compared based on their phenotypic (physiological and biochemical) characteristics and pathogenicity tests. Pss strains showed slight differences in phenotypic properties. All 23 strains were highly pathogenic on almond and peach seedlings. All strains appeared to be similar in pathogenicity traits. Identification of the strains was further confirmed by PCR using D21/D22 primers specific to P. syringae. Genetic diversity of selected strains was investigated by repetitive element palindromic PCR (rep-PCR) fingerprinting using BOXA1R, ERIC1R, ERIC2 primers and Insertion Sequence PCR (IS-PCR) fingerprinting using IS50 primers. The cluster analyses of the fingerprint data showed that in BOX-PCR with BOXA1R primer, 7 groups were developed at a similarity level of 75%. The same number of groups were obtained in ERIC-PCR using ERIC1R primer at a similarity level of 65%. In case of ERIC2 primer, at a similarity level of 56%, strains were divided into 8 groups, whereas, in IS-PCR with IS50 primer at a similarity level of 52%, strains were divided into 11 groups and finally by combining all four primers at a similarity level of 54%, strains can be distinguished into 7 groups. The results demonstrated the existence of a considerable genetic diversity among Pss strains causing bacterial canker in stone fruit trees in the studied provinces. | ||
| کلیدواژه‌ها [English] | ||
| Bacterial canker, Genetic diversity, Stone fruit trees, rep-PCR, IS-PCR | ||
| مراجع | ||
|
ABBASI, V., RAHIMIAN, H. and TAJICK-GHANBARI, A., 2013a. Identification and genetic diversity of Pseudomonas syringae pv. syringae strains causing bacterial canker in stone fruits in several northern and central provinces. Journal of Plant Production Research 20(3): 27-48. ABBASI, V., RAHIMIAN, H. and TAJICK-GHANBARI, A., 2013b. Genetic variability of Iranian strains of Pseudomonas syringae pv. syringae causing bacterial canker disease of stone fruits. European Journal Plant Pathology 135: 225–235 ABBASI, V., RAHIMIAN, H., TAJICK-GHANBARI, M. and REZAIAN, V., 2011. Assessment of genetic diversity of strains of Pseudomonas syringae pv. syringae causing bacterial canker in stone fruits in some northern provinces of Iran. Iranian Journal of Plant Pathology 47(4): 389-404. AGRIOS, G.N., 2005. Plant pathology. 5th ed. Elsevier Academic Press. ARABI, F., NIKRAVESH, Z., BABAEIZAD, V., REZAEIAN, V. and RAHIMIAN, H., 2006. Occurrence of bacterial blight disease caused by Pseudomonas syringae pv. aptata in Iran. Iranian Journal of Plant Pathology 42(1): 665-669. BAHAR, M., MOJTAHEDI, H. and AKHYANI, A., 1982. Apricot bacterial cancer in Isfahan. Iranian Journal of Plant Pathology 18(3): 58-68. BANAPUR, A., ZAKAI, Z. and AMANI, G., 1989. Isolation of Pseudomonas syringae from cherry trees in Tehran province. Iranian Journal of Plant Pathology 26(1): 67-72. BERG, D.E. and HOWE, M.M., 1989. Mobile DNA. Washington. D.C: American Society of Microbiology Publication. BULTREYS, A. and KALUZNA, M., 2010. Bacterial cankers caused by Pseudomonas syringae on stone fruit species with special emphasis on the pathovars syringae and morsprunorum race1 and race2. Journal of Plant Pathology 92: 21-33. DAWSON, S.L., FRY, J.C. and Dancer, B.N., 2002. A comparative evaluation of five typing techniques for determining the diversity of fluorescent Pseudomonas. Journal of Microbiological Methods 50: 9-22. GILBERT, V., LEGROS, F., MARAITE, H., and BULTREYS, A., 2009. Genetic analyses of Pseudomonas syringae isolates from Belgian fruit orchards reveal genetic variability and isolate-host relationships within the pathovar syringae, and help identify both races of the pathovar morsprunorum. European Journal of Plant Pathology 124: 199-218. GONZALEZ, A.I., PEREZ, D.E. L.A. VEGA, M., RUIZ, M.L. and POLANCO C., 2003. Analysis of the arg K-tox gene cluster in nontoxigenic strains of Pseudomonas syringae pv. phaseolicola. Applied and Environmental Microbiology 69: 4979-4982. KANG, H.P. and DUNNE, W.M., 2003. Stability of repetitive-sequence PCR patterns with respect to culture age and subculture frequency. Journal of Clinical Microbiology 41: 2694-2696. KENNELLY, M.M., CAZORLA, F.M., VICENTE, A., RAMOS, C. and SUNDIN, G.W., 2007. Pseudomonas syringae diseases of fruit trees progress toward understanding and control. Plant disease 91(1): 4-17. LITTLE, E.L., BOSTOCK, R.M. and KIRKPATRIC, B.C., 1998. Genetic characterization of Pseudomonas syringae pv. syringae from stone fruits in California. Applied and Environmental Microbiology 64: 3818-3823. LOUWS, F.G., FULBRIGHT, D.W., STEPHANS, C.T. and BRUJIN, F.G., 1994. Spesific genomic fingerprinting of phytopathogenic Xanthomonas and Pseudomonas pathovar and strains generated whit repetitive sequences and PCR. Applied and Environmental Microbiology 60: 2286-2299. LOUWS, F.G., RADEMARKER, J.L.W. and BRUIJN, F.J., 1999. The three Ds of PCR-Base genoimic analysis of phytobacterial diversity, detection and diagnosis. Annual Review of Phytopathology 37: 81-125. MAHILLON, J. and CHANDLER, M., 1998. Insertion sequences. Microbiology and Molecular Biology Reviews 62: 725-744. MAJD, A. and SHARIATZADEH, M.A., 2015. Molecular cell biology. Ayiizh Publications. MANCEAU, C. and HORVAIS, A., 1997. Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-Restriction fragment length polymorphism analysis of rRNA operons with special emphasis on Pseudomonas syringae pv. tomato. Applied and Environmental Microbiology 63: 491-505. MENARD, M., SUTRA, L., LUISETTI, J., PRUNIER, J.P., and GARDAN, L., 2003. Pseudomonas syringae pv. avii the causal agent of bacterial canker of wild cherries (Prunus avium) in France. European Journal of Plant Pathology 109: 565-576. MILGROOM, M.G. and FRY, W.E., 1997. Contribution of population genetics to plant disease epidemiology and management. Review of Plant Pathology 59: 153-168. MOHAMADNIA, A., BAHRAMI, N., TASHAKOR, A., JAHANBAZI, A. and VAZIRITABAR N, 2015. Molecular cell biology, Lodish (translation and editing). Shahid Beheshti University of Medical Sciences Publications. MOSIVAND, M., RAHIMIAN, H. and SHAMS-BAKHSH, M., 2009. Phenotypic and genotypic relatedness among Pseudomonas syringae pv. syringae strains isolated from sugarcane, stone fruits and wheat. Iranian Journal of Plant Pathology 45(1): 85-98. NAJAFI POUR, G. and TAGHAVI, M., 2011. Comparison of Pseudomonas syringae pv. syringae from different hosts based on pathogenicity and BOX-PCR in Iran. Journal of Agricultural Science and Technology 13: 431-442. PALACIO-BIELSA, A., CAMBRA, M.A. and LOPEZ, M.M., 2009. PCR detection and identification of plant-pathogenic bacteria: updated review of protocols (1989-2007). Journal of Plant Pathology 91(2): 249-297. PALLERONI, N.J., 2005. Genus Ӏ. Pseudomonas. Pp. 33-379 In: Brenner DJ, Krieg NR and Staley JT (eds.) Bergey᾽s manual of systematic bacteriology. Springer, New York, USA. ROHLF, F.J, 1990. NTSYS-pc. Numerical taxonomy and multivariat analysis system, version 2.02. Applied Biostatistics Inc. Exeter Software, New York, USA. ROOS, I.M. and HATTINGH, M.J., 1987. Systematic invasion of plum leaves and shoote by Pseudomonas syringae pv. syringae introduced into petioles. Journal of Phytopathology 77: 1253-1257. SCHAAD, N.W., JONES, J.B., and CHUN, W., 2001. Laboratory guide for identification of plant pathogenic bacteria. 3th ed. APS Press. THOMIDIS, T., TSIPOURIDIS, C., EXADAKTYLOU, E. and DROGOUDI, P., 2005. Comparison of three laboratory methods to evaluate the pathogenicity and virulence of ten Pseudomonas syringae pv. syringae strains on apple, pear, cherry and peach trees. Phytopathology 33: 137-140. VERSALOVIC, J., SCHEIDER, M., BRUIJN, F.J. and LUPSKI, J.R., 1994. Genomic fingerprinting of bacteria using repetitive sequence based polymerase chain reaction. Methods in Molecular and Cellular Biology 5: 25-40. VICENT, J.G., ALVES, J.P., RUSSELL, K. and ROBERTS S.J., 2004. Identification and discrimination of Pseudomonas syringae isolates from wild cherry in England. European Journal of Plant Pathology 110: 337-351. VICENT, J.G. and ROBERTS, S.J., 2007. Discrimination of Pseudomonas syringae isolated from sweet and wild cherry using rep-PCR. European Journal of Plant Pathology 117: 383-392. WEINGART, H. and VOLKSCH, B., 1997. Genetic fingerprinting of Pseudomonas syringae pathovars using ERIC, REP and IS_PCR. Journal of Phytopathology 145: 339-345. WOLSKA, K., SZWEDA, P., LADA, K., RYTEL, E., GUCWA, K., KOT, B. and PIECHOTA, M., 2014. Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment).Journal of Veterinary Sciences 17(2): 321–329. | ||
|
آمار تعداد مشاهده مقاله: 501 تعداد دریافت فایل اصل مقاله: 382 |
||