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مطالعه هموگرام و تاثیر تنش دما بر فراوانی ایمنوسیتهای کرم جگری Cossus cossus (Lepidoptera: Cossidae) | ||
| تحقیقات حمایت و حفاظت جنگلها و مراتع ایران | ||
| مقاله 9، دوره 17، شماره 2 - شماره پیاپی 34، اسفند 1398، صفحه 239-250 اصل مقاله (525.65 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijfrpr.2020.127752.1395 | ||
| نویسندگان | ||
| مریم عجم حسنی* 1؛ زهرا پورعلی2 | ||
| 1دانشگاه صنعتی شاهرود | ||
| 2فارغ التحصیل کارشناسی ارشد دانشگاه صنعتی شاهرود | ||
| چکیده | ||
| ورود عوامل بیمارگر، عفونتها و آلاینده های محیط به همولنف حشرات، تنشهای دمایی و تغییرات رژیم غذایی، سامانه ایمنی حشرات را تحت تاثیر قرار می دهد. ایمنی حشرات شامل مولفه هایی است که سلولهای خونی اجزای اصلی آن محسوب می شوند. در تحقیق حاضر، مطالعه مرفولوژی و تغییرات تراکم سلولهای خونی در همولنف کرم جگری در برابر تنشهای دمایی ملاک کار قرار گرفت. سلولهای خونی مراحل مختلف لاروی پروانه کرم جگری Cossus cossus پس از رنگ آمیزی با گیمسا مورد بررسی قرار گرفت و واکنش این سلولها در برابر دماهای 4 و 30 درجه سلسیوس ارزیابی شد. پنج نوع هموسیت شامل پروهموسیتها، گرانولوسیتها، پلاسموتوسیتها، اونوسیتوئیدها و اسفرولوسیتها در لاروها شناسایی شد. ولی پراکندگی آنها در مراحل مختلف لاروی، متفاوت بود. شمارش کل سلولهای خونی نشان داد که با افزایش سن لاروی و جثه بدن، به تدریج بر تعداد سلولهای خونی و حجم همولنف افزوده میشود. شمارش تفرقی سلولهای خونی نشان داد که پلاسموتوسیتها و گرانولوسیتها در لاروهای سنین پنجم و ششم بیشترین فراوانی را نسبت به سایر مراحل رشدی داشتند. گرانولوسیتها در برابر تنش گرما و سرما بیشترین تغییرات فراوانی را نشان دادند و پلاسموتوسیتها نیز تحت تاثیر سرما به طور معنی داری کاهش یافتند. به نظر می رسد شناخت ویژگیهای هموسیتهای این آفت چوبخوار و تغییرات فراوانی ایمنوسیتها در مقابل تنشهای دمایی بتواند به عنوان مقدمه ای بر مطالعات ایمنی شناسی این آفت قلمداد شود. قطعا جهت بررسی برهمکنش سیستم ایمنی کرم جگری با عوامل بیمارگر و سنجش به صرفه بودن کنترل میکروبیولوژیک، نیاز به تحقیقات تکمیلی می باشد. | ||
| کلیدواژه‌ها | ||
| کرم جگری؛ هموسیت‌ها؛ تنش دمایی؛ ایمنی شناسی | ||
| عنوان مقاله [English] | ||
| Hemogram study and effect of thermal Stresses on abundance of immunocytes in larvae of Goat Moth, Cossus cossus (Lepidoptera: Cossidae) | ||
| نویسندگان [English] | ||
| Maryam Ajamhassani1؛ zahra pourali2 | ||
| 1Shahrood University of Technology | ||
| 2Shahrood university of technology | ||
| چکیده [English] | ||
| The introduction of pathogens, infections and environmental pollutants into the insect hemolymphs, thermal stresses and nutritional diet changes, affect the insect immune system. Insect immunity consists of components that hemocytes are the main components of them. In the present study, the morphology and changes in density of hemocytes in the hemolymph of the Cossus cossus L. were studied against thermal stresses. The hemocytes of all larval stages of C. cossus were examined by Giemsa and the response of these cells was evaluated at temperatures of 4 °C and 30 °C. Five identified types of hemocyte in this insect were; Prohemocyte, Plasmatocyte, Granulocyte, Oenocytoide and Spherulocyte. All hemocytes types were detected in each stage but frequency of them in each stage was different. Total Hemocyte Count showed that by increasing larval instars and body size, hemocyte rate increased gradually. Differential count hemocyte indicated that plasmatocytes and granulocytes are the most abundance in 5th and 6th instars larvae. So 5th and 6th instars larvae have the highest blood volum and hemocyte count. Granulocytes showed the highest abundance variation in heat and chill stress, and plasmatocytes also decreased significantly under the influence of chill stress. It seems that recognizing the hemocyte characteristics of this pest and the frequency of immunocyte changes against to thermal stresses, can be considered as an introduction on immunological studies of this pest. Surely, further investigation is needed to investigate the interaction of the immune system of C. cossus with pathogens and to evaluate the efficacy of microbiological control. | ||
| کلیدواژه‌ها [English] | ||
| : Goat Moth, hemocytes, thermal stress, Immunology | ||
| مراجع | ||
|
-Ajamhassani, M., Sendi, J.J., Zibaee, A., Askary, H. and Farsi, M.J. 2013. Immunoliogical Responses of Hyphantria Cunea (Drury) (Lepidoptera: Arctiidae) to Entomopathogenic Fungi, Beauveria Bassiana (Bals.-Criy) and Isaria Farinosae (Holmsk.) Fr. Journal of Plant Protection Research, 53: 110-118. -Ajamhassani, M. 2015. Study of cytology of hemocytes in the Spurge hawk-moth, Hyles euphorbiae L. (Lepidoptera: Sphingidae). Plant Protection (Agricultural Science Journal), 38(3): 49-62. -Ajamhassani, M. and Mahmoodzadeh, M. 2019. Cellular defense responses of 5th instar larvae of the Apple Ermine Moth, Yponomeuta malinellus (Lepidoptera: Yponomeutidae) against starvation, thermal stresses and entomopathogenic bacteria Bacillus thuringiensis. Journal of Animal Researches, 4(2): 59-68. -Ajamhassani, M. 2019. Study on morphology and frequency of hemocytes in Osphranteria coerulescense (Redt) (Coleoptera: Cerambycidae) and Zeuzera pyrina L. (Lepidoptera: Cossidae) larvae, two wood boring insects of Iran. Accepted inIranian Journal of Forest and Range Protection Research, 17(2): 96-106. -Andrade, F.G.D., Negreiro, M. C.C.D., Levy, S.M., Fonseca, I.C.D. B., Moscardi, F. and Falleiros, Â.M.F. 2010. Hemocyte quantitative changes in Anticarsiagemmatalis (Lepidoptera: Noctuidae) larvae infected by AgMNPV. Brazilian Archives of Biology and Technology, 53(2): 279-284. -Ashida, M., Ishizaki, Y. and Iwahana, H. 1983. Activation of pro-phenoloxidase by bacterial cell walls or beta-1, 3-glucans in plasma of the silkworm, Bombyx mori. Biochemical and Biophysical Research Communications, 113: 562-568. -Bao, Y., Yamano, Y. and Morishima, I. 2007. Induction of hemolin gene expression by bacterial cell wall components in eri-silkworm, Samia cynthiaricini. Molecular Biology, 146: 147−151. -Beaulaton, J. 1979. Hemocytes and hemocytopoiesis in silkworms. Biochemistry, 61: 157-164. -Bulet, P. and Stöcklin, R. 2005. Insect antimicrobial peptides: structures, properties and gene regulation. Protein Pept Lett, 12(1): 3-11. -Correia, A.A. 2008. Histofisiologia do canal alimentar e hemócitos de Spodoptera frugiperda (JE Smith) (Lepdoptera: Noctuidae) tratadas com nim (Azadirachta indica A. Juss). Dissertation, Universidade Federal Rural de Pernambuco. -Ghasemi, V., Moharramipour, S. and Jalali Sendi, J. 2013. Circulating hemocytes of Mediterranean flour moth, Ephestia kuehniella Zell.(Lep: Pyralidae) and their response to thermal stress. Invertebrate Survival Journal, 10: 128-140. -Gillespie, J.P., Burnett, C. and Charnley, A.K. 2000. The immune response of the desert locust Schistocerca gregaria during mycosis of the entomopathogenic fungus, Metarhizium anisopliae var acridum. Journal of Insect Physiology, 46: 429-437. -Gupta, A.P. 1985.Cellular elements in the hemolymph. In: Kerkut, G.A., Gilbert, L. I. (Eds.), Comperhensive Insect Physiology, Biochemistry and Pharmacology. Cambridge University Press, pp. 85-127. -Hannon, E.R., Rodstrom, R.A., Chong, J.M. and Brown, J.J. 2017. Carpenterworm Moth. Washington State University Extension. -Iwama, R. and Ashida, M. 1986. Biosynthesis of prophenoloxidase in hemocytes of larval hemolymph of the silkworm, Bombyx mori. Insect biochemistry, 16(3): 547-555. -Jalali, J. and Salehi, R. 2008. The hemocyte types, differential and total count in Papiliodemoleus L. (Lepidoptera: Papilionidae) during post-embryonic development. Munis Entomology & Zoology Journal, 1: 199-216. -Jiang, H., Wang, Y., Ma, C. and Kanost, M.R. 1997. Subunit composition of pro-phenol oxidase from Manduca sexta: molecular cloning of subunit ProPO-P1. Insect biochemistry and molecular biology, 27(10): 835-850. -Jones, J.C. 1962. Current concepts concerning insect hemocytes. American Zoologist, 2: 209-246. -Jones, J.C. 1967. Changes in the haemocyte picture of Galleriamellonella (Linnaeus). Biology Bulletin, 132: 211-221. -Khosravi, R., Jalali Sendi, J. and Ghasemi, V. 2012. Identification of hemocytes in carob moth, Ectomoyeloisceratoniae Zeller (Lepidoptera: Pyralidae) larvae. Plant Pests Research , 2: 29-39. -Lakshmi, K. and Gupta, P. 1987.Variation in hemocyte populations during various developmental stages of Blatella germanica (L.) (Dictyoptera, Blattellidae). Zoology Science, 4: 307-313. -Ling, E., Shirai, K., Kanekatsu, R. and Kiguchi, K. 2005. Hemocyte differentiation in the hematopoietic organs of the silkworm, Bombyx mori: prohemocytes have the function of phagocytosis. Cell and Tissue Research, 320: 535-543. -Mowlds, P. and Kavanagh, K. 2008. Effect of pre-incubation temperature on susceptibility of Galleriamellonella larvae to infection by Candida albicans. Mycopathologia, 165: 5-12. -Nardi, J.B., Pilas, B., Ujhelyi, E., Garsha, K. and Kanost, M.R. 2003. Hematopoetic organs of Manduca sexta and hemocyte lineages. Development Genes and Evolution, 213: 477-491. -Pandey, J.P., Tiwari, R.K. and Kumar, D. 2008 a. Temperature and ganglionectomy stresses affect haemocyte counts in plain tiger butterfly, Danais chrysippus L.(Lepidoptera: Nymphalidae). Journal of Entomology, 5(2): 113-121. -Pandey, J.P., Tiwari, R.K. and Kumar, D. 2008 b. Reduction in haemocyte mediated immune response in Danaus chrysippus following treatment with neem based insecticides. Journal of Entomology, 5: 200-206. -Pandey, J.P., Mishra, P.K., Kumar, D., Singh, B.M.K. and Prasad, B.C. 2010. Effect of temperature on hemocytic immune responses of tropical tasar silkworm, Antheraea mylitta D. Research Journal of Immunology, 3(2): 169-177. -Parmakelis, A., Slotman, M.A., Marshall, J.C., Awono-Ambene, P.H. and Antonio-Nkondjio, C. 2009. The molecular evolution of four anti-malarial immune genes in the Anopheles gambiae species complex. BMC Evolutionary Biology, 8: 79. -Pourali, Z. and Ajamhassani, M. 2018. The effect of thermal stresses on the immune system of the potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae). Journal of Entomological Society of Iran.Supplementary, 37(4): 515-525. -Shapiro, M. 1979. Changes in hemocyte populations. In: Gupta AP (ed.), Insect hemocytes, Cambridge University Press, 475-524. -Söderhäll, K. and Cerenius, L. 1998. Role of the prophenoloxidase-activating system in invertebrate immunity. Current opinion in immunology, 10(1): 23-28. -Strand, M.R. 2008. The insect cellular immune response. Journal Insect Science, 15: 1-14. -Terra, W. R., Bianchi, A. G. and Lara, F. J. S. 1975. Physical properties and chemical composition of the haemolymph of Rhynchosciara Americana (Diptera) larvae. Comprative Biochemistry and Physiology, 47: 117-129. -Valadez-Lira, J.A., Gonzalez, J.M., Damas, G., Meja, G., Oppert, B., Padilla C. and Guerra, P. 2011. Comparative evaluation of phenoloxidase activity in different larval stages of four lepidopteran after exposure to Bacillus thuringiensis. Journal of Insect Science, 12(80): 1-11. -Yamashita, M. and Iwabuchi, K. 2001. Bombyx mori prohemocyte division and differentiation in individual microcultures. Journal of insect physiology, 47(4-5): 325-331. -Yeager, J.F. 1945.The blood picture of the southern armyworm (Prodenia eridania). Journal of Agricultural Research, 71: 1-40. | ||
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