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تعیین بهترین محیط‌کشت جهت ریزازدیادی سرو کهنسال ابرکوه (Cupressus sempervirens var. horizontalis) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 7، دوره 27، شماره 2 - شماره پیاپی 54، اسفند 1398، صفحه 240-251 اصل مقاله (915.75 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2019.122188.1307 | ||
| نویسندگان | ||
| الهه زمانی1؛ مریم دهستانی اردکانی* 2؛ کاظم کمالی3 | ||
| 1دانشجوی کارشناسی ارشد گروه علوم باغبانی دانشکده کشاورزی و منابع طبیعی دانشگاه اردکان، ایران | ||
| 2استادیار گروه علوم باغبانی دانشکده کشاورزی و منابع طبیعی دانشگاه اردکان، یزد، ایران | ||
| 3استادیار گروه علوم خاکشناسی دانشکده کشاورزی و منابع طبیعی دانشگاه یزد، ایران | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1398.27.241.54.2.1578.41 این پژوهش بهمنظور حفظ ذخیره ژنتیکی و تکثیر سرو کهنسال ابرکوه (Cupressus sempervirens var. horizontalis Mill.) به روش کشت بافت انجام شد. در این تحقیق، تأثیر محیطهای کشت مختلف شاملWPM (Woody Plant medium)، WPMتغییریافته، LS، SH و QL همراه با تنظیم‌کننده بنزیل آدنین (BA) در مقادیر مختلف 0، 01/0 و 1 میلیگرم بر لیتر در مرحله استقرار و 0، 1، 5/1، 2 و 5 میلیگرم بر لیتر در مرحله پرآوری در کشت شیشهای این گیاه بررسی شدند. ریشهزایی، با دو روش کشت درون و برون شیشهای مطالعه شد. در کشت برون شیشهای از ترکیب کوکوپیت و پرلایت با سه نسبت 1:1، 2:1 و 3:1 استفاده شد. پس از تیمار انتهای گیاهچههای حاصل از کشت بافت با چهار غلظت IBA (1000، 2000، 3000 و 4000 میلیگرم بر لیتر) درون بسترها کشت شدند. در کشت درون شیشهای، ریزنمونهها بهمنظور تولید ریشه در محیط کشت WPM حاوی مقادیر مختلف IBA (1، 2 و 5 میلیگرم بر لیتر) کشت گردیدند. طبق نتایج بهدست‌آمده در هر دو مرحله استقرار و پرآوری، بیشترین رشد طولی ریزنمونهها در محیط کشت WPM حاصل شد. در مرحله پرآوری، بیشترین تعداد شاخه در محیط کشت LS بهدست آمد که با محیطهای WPM و WPM تغییر یافته اختلاف معنیداری نشان نداد. با افزایش غلظت BA، تعداد و طول شاخساره بهطور معنیداری افزایش یافت. بهطوریکه بیشترین تعداد و طول شاخه بهترتیب در غلظت 5 و 2 میلیگرم در لیتر BA حاصل شد. ریشهزایی تنها در ریزنمونههای کشت شده در محیط کشت WPM حاوی 5 میلیگرم در لیتر IBA انجام گردید. | ||
| کلیدواژه‌ها | ||
| تنظیم کننده‌های رشد گیاهی؛ پرآوری؛ کشت بافت؛ ریزنمونه؛ ریشهزایی | ||
| عنوان مقاله [English] | ||
| Determination of the best culture medium for micropropagation of old Abarkooh cypress plant (Cupressus semprvirens var. horizontalis) | ||
| نویسندگان [English] | ||
| Elahe Zamani1؛ Maryam Dehestani-Ardakani2؛ Kazem Kamali3 | ||
| 1M.Sc. student, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran. | ||
| 22- Assistant Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran | ||
| 3Assistant Professor, Department of Soil Science, Faculty of Agriculture & Natural Resources, Yazd University, Yazd, Iran | ||
| چکیده [English] | ||
| This study was conducted to preserve the genetic reserve and proliferation of old cypress of Abarkooh, (Cupressus semprvirens var. horizontalis Mill.) in Iran via tissue culture method. In this research, the effect of different culture media including WPM (woody plant medium), modified WPM, LS, SH and QL supplemented with Benzyl-adenine (BA) at different concentrations (0, 0.01 and 1 mg/l) at establishment stage and 0, 1, 1.5, 2 and 5 mg/l at proliferation phase were investigated through in vitro cultivation of this plant. Rooting was studied by two methods: in vitro and in vivo culture conditions. For in vivo culture method, three ratios of coccopeat and perlite containing 1:1, 1:2 and 1:3 were used. After treatment of the end of in vitro cutting explants with four concentrations of IBA (1000, 2000, 3000 and 4000 mg/l), cutting clones were cultured in the prepared media. Under in vitro culture conditions, explants were cultured in WPM medium supplemented by different concentrations of IBA (1, 2 and 5 mg/l) for root production. According to the results obtained at both phases of establishment and proliferation, the highest length of explants was obtained in WPM medium. In the proliferation phase, the maximum number of shoots was obtained in LS medium that had no significant differences with WPM and modified WPM media. By increasing the BA concentration, the number and length of shoots significantly increased. The maximum number and length of shoots were obtained at 5 and 2 mg/l BA, respectively. Only explants in WPM medium containing 5 mg/l IBA were successfully rooted. | ||
| کلیدواژه‌ها [English] | ||
| Plant Growth Regulations, Proliferation, Tissue culture, Explant, Rooting | ||
| مراجع | ||
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