| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,829 |
| تعداد مقالات | 32,098 |
| تعداد مشاهده مقاله | 40,864,071 |
| تعداد دریافت فایل اصل مقاله | 18,113,688 |
تعیین بهترین محیط‌کشت جهت ریزازدیادی سرو کهنسال ابرکوه (Cupressus sempervirens var. horizontalis) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 7، دوره 27، شماره 2 - شماره پیاپی 54، اسفند 1398، صفحه 240-251 اصل مقاله (915.75 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2019.122188.1307 | ||
| نویسندگان | ||
| الهه زمانی1؛ مریم دهستانی اردکانی* 2؛ کاظم کمالی3 | ||
| 1دانشجوی کارشناسی ارشد گروه علوم باغبانی دانشکده کشاورزی و منابع طبیعی دانشگاه اردکان، ایران | ||
| 2استادیار گروه علوم باغبانی دانشکده کشاورزی و منابع طبیعی دانشگاه اردکان، یزد، ایران | ||
| 3استادیار گروه علوم خاکشناسی دانشکده کشاورزی و منابع طبیعی دانشگاه یزد، ایران | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1398.27.241.54.2.1578.41 این پژوهش بهمنظور حفظ ذخیره ژنتیکی و تکثیر سرو کهنسال ابرکوه (Cupressus sempervirens var. horizontalis Mill.) به روش کشت بافت انجام شد. در این تحقیق، تأثیر محیطهای کشت مختلف شاملWPM (Woody Plant medium)، WPMتغییریافته، LS، SH و QL همراه با تنظیم‌کننده بنزیل آدنین (BA) در مقادیر مختلف 0، 01/0 و 1 میلیگرم بر لیتر در مرحله استقرار و 0، 1، 5/1، 2 و 5 میلیگرم بر لیتر در مرحله پرآوری در کشت شیشهای این گیاه بررسی شدند. ریشهزایی، با دو روش کشت درون و برون شیشهای مطالعه شد. در کشت برون شیشهای از ترکیب کوکوپیت و پرلایت با سه نسبت 1:1، 2:1 و 3:1 استفاده شد. پس از تیمار انتهای گیاهچههای حاصل از کشت بافت با چهار غلظت IBA (1000، 2000، 3000 و 4000 میلیگرم بر لیتر) درون بسترها کشت شدند. در کشت درون شیشهای، ریزنمونهها بهمنظور تولید ریشه در محیط کشت WPM حاوی مقادیر مختلف IBA (1، 2 و 5 میلیگرم بر لیتر) کشت گردیدند. طبق نتایج بهدست‌آمده در هر دو مرحله استقرار و پرآوری، بیشترین رشد طولی ریزنمونهها در محیط کشت WPM حاصل شد. در مرحله پرآوری، بیشترین تعداد شاخه در محیط کشت LS بهدست آمد که با محیطهای WPM و WPM تغییر یافته اختلاف معنیداری نشان نداد. با افزایش غلظت BA، تعداد و طول شاخساره بهطور معنیداری افزایش یافت. بهطوریکه بیشترین تعداد و طول شاخه بهترتیب در غلظت 5 و 2 میلیگرم در لیتر BA حاصل شد. ریشهزایی تنها در ریزنمونههای کشت شده در محیط کشت WPM حاوی 5 میلیگرم در لیتر IBA انجام گردید. | ||
| کلیدواژه‌ها | ||
| تنظیم کننده‌های رشد گیاهی؛ پرآوری؛ کشت بافت؛ ریزنمونه؛ ریشهزایی | ||
| عنوان مقاله [English] | ||
| Determination of the best culture medium for micropropagation of old Abarkooh cypress plant (Cupressus semprvirens var. horizontalis) | ||
| نویسندگان [English] | ||
| Elahe Zamani1؛ Maryam Dehestani-Ardakani2؛ Kazem Kamali3 | ||
| 1M.Sc. student, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran. | ||
| 22- Assistant Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran | ||
| 3Assistant Professor, Department of Soil Science, Faculty of Agriculture & Natural Resources, Yazd University, Yazd, Iran | ||
| چکیده [English] | ||
| This study was conducted to preserve the genetic reserve and proliferation of old cypress of Abarkooh, (Cupressus semprvirens var. horizontalis Mill.) in Iran via tissue culture method. In this research, the effect of different culture media including WPM (woody plant medium), modified WPM, LS, SH and QL supplemented with Benzyl-adenine (BA) at different concentrations (0, 0.01 and 1 mg/l) at establishment stage and 0, 1, 1.5, 2 and 5 mg/l at proliferation phase were investigated through in vitro cultivation of this plant. Rooting was studied by two methods: in vitro and in vivo culture conditions. For in vivo culture method, three ratios of coccopeat and perlite containing 1:1, 1:2 and 1:3 were used. After treatment of the end of in vitro cutting explants with four concentrations of IBA (1000, 2000, 3000 and 4000 mg/l), cutting clones were cultured in the prepared media. Under in vitro culture conditions, explants were cultured in WPM medium supplemented by different concentrations of IBA (1, 2 and 5 mg/l) for root production. According to the results obtained at both phases of establishment and proliferation, the highest length of explants was obtained in WPM medium. In the proliferation phase, the maximum number of shoots was obtained in LS medium that had no significant differences with WPM and modified WPM media. By increasing the BA concentration, the number and length of shoots significantly increased. The maximum number and length of shoots were obtained at 5 and 2 mg/l BA, respectively. Only explants in WPM medium containing 5 mg/l IBA were successfully rooted. | ||
| کلیدواژه‌ها [English] | ||
| Plant Growth Regulations, Proliferation, Tissue culture, Explant, Rooting | ||
| مراجع | ||
|
- Al-Ramamneh, E.A., Durra, S., and Daradkeh, N. 2012. Propagation physiology of Juniperus phoenicea L. from Jordan using seeds and in vitro culture techniques: baseline information for a conservation perspective. African Journal of Biotechnology, 11: 7684-7692. - Asrar Zaidi, M. S., Khan, N., Jahan, A., Yousafzai and Mansoor, A., 2012. Micropropagation and Conservation of Three Juniperus Specices (cuperssaceae). Pakistan journal of Botany, 44: 301-304. - Al-Ramamneh, E.A., Daradkeh, N., Rababah, T., Pacurar, D. and Al-Qudah, M. 2017. Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea. Australian Journal of Crop Science, 11(7): 828-837. - Andersone, U. and Ievinsh, G., 2002. Changes of morphogenic competence in mature Pinus sylvestris L. buds in vitro. Annals of botany, 90 (2): 293-298. - Bagheri, H., and Azadi, P. 2002. Plant Tissue Culture, Techniques and Experiments. Mashhad jehad. Daneshgahi Press. 154p.(In Persian). - Behjat Sasan, B., Omidi, M., Naghavi, M. R., Hariri Akbari, F., Kalate Jari, S., Shafiee, M., and Shafiee, M. 2014. Effect of explants, salts concentration medium and hormone treatments on Taxus baccata in vitro culture. International Journal of Biosciences. 5(6): 1-9. - Bhojwani, S.S. and Dantu, P.K., 2013. Plant tissue culture: an introductory text (pp. 245-274). India: Springer. - Bolandi, A., Hamidi, H. and Rezagholy, A. A., 2016. Effects of culture media and growth regulators on propagation of rootstock GF677 in tissue culture conditions, Journal of Plant Research, 29 (1): 1-14. - Bornman, C.H. and Vogelmann, T.C. 1984. Effect of rigidity of gel medium on benzyladenine‐induced adventitious bud formation and vitrification in vitro in Picea abies. Physiologia Plantarum, 61 (3): 505-512 - Capuana, M. and Giannini, R. 2015. Micropropagation of young and adult plants of cypress (Cupressus sempervirens L.). Journal of Horticultural Science, 72 (3): 453-460. - Chee, P.P. 1995. Organogenesis in Taxus brevifolia tissue cultures. Plant cell reports, 14(9): 560-565. - Fernandez-Lorenzo, J.L., Iglesias-Díaz, M.I. and Gutiérrez-Araujo, O. 2000. September. Micropropagation of a selected rootstock of Acer palmatum. In XIVth International Symposium on Horticultural Economics. 536: 347-354. - Emam, M. 2003. In vitro propagation of Thuja orientalis by shoot tip culture. Rangelands and Forests Plant Breeding and Genetic Research. 11(1): 1-16. (In Persian). - George, E.F., Hall, M.A. and De Klerk, G.J. 2008. Plant Propagation by Tissue Culture. Springer, Dordrecht, The Netherlands, 3rd ed, Vol. 1. 501p. - Giovanelli, A. and De Carlo, A. 2007 Micropropagation of Mediterranean cypress (Cupressus sempervirens L.). In: Mohan Jain, S., Haggman, H., (eds) Protocols for micropropagation of woody trees and fruits. Springer, The Netherlands, pp 93–105. - Ghamari Zare, A., Sedaghati, M., Imam, M., Assareh, M. and Kiarostami, Kh. 2013. Eucalyptus micropropagation from adult rootstock via in vitro culture. Journal of Forest and Poplar Research, 21 (3): 593-581. (In Persian) - Irannejad Parizi, M., Akbari, H., Khoshnevis, M., Shams, J., Abedinin, T., Hadirad, M., Rasouli, A., Taheri, A. and Mahdavi, M. 2016. National Monument of Abarkooh cypress. Yazd university publication, 306p (In Persian). - Jafari Mofidabadi, A., Kamandloo, M. and Nazari, A. 2018. Effect of juvenile tissue and plant growth regulators on in vitro shoot proliferation of Pronus avium L. Rangelands and Forests Plant Breeding and Genetic Research. 26(1): 114-120. doi: 10.22092/ijrfpbgr.2018.116665 (In Persian). - Khoshnevis, M., Matinizade, M., Shirvani, A. and Taimouri, M. 2017. Iran treasure of ancient trees. Nature of Iran, 2 (3): 42-55 (In Persian). - Linsmaier, E.M. and Skoog, F. 1965. Organic growth factor requirments of tobacco tissue culture. Physiology of Plant, 18: 100-127. - Loureiro, J., Capelo, A., Brito, G., Rodriguez, E., Silva, S., Pinto, G., and Santos, C. 2007. Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry. Biol Plantarum. 51: 7-14. - Lu, M.C. 2005. Micropropagation of Vitis thunbergii Sieb. et Zucc., a medicinal herb, through high-frequency shoot tip culture. Scientia Horticulturae, 107(1): 64-69. - Mahdinejad, N., Fakheri, B.A. and Ghanbari, S. 2015. Effect of growth regulators on in vitro callogensis Taxus baccata L. Biological Forum–An International Journal, 7(1): 142-145. - Mihaljevic, S., Bjedou, I., Kovac, M., Levanic, D.L. and Jelaska, S. 2002. Effect of explants source and growth regulators on in vitro callus growth of Taxus baccata L. Washingtonii. Food Technology and Biotechnology, 40(4), 299-303. - McCown, B.H. and Lloyd, G. 1981. Woody plant medium (WPM) – a mineral nutrient formulation for microculture of woody plant species. HortScience, 16: 453-453. - Morley, P.S. 2016. David J. Pilbeam. Plant Nutrition, 121 p. - Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3): 473-497. - Neumann, K.H., Kumar, A., Imani, J. 2009. Plant Cell and Tissue Culture - A Tool in Biotechnology, Basics and Application. Springer, Berlin, Heidelberg, 322 p. - Pasqual, M. and Ferreira, E.A. 2007. Micropropagation of Fig tree (Ficus carica sp). In Protocols for micropropagation of woody trees and fruits (pp. 409-416). - Quoirin, M. and Lepoivre, P. 1977. Improved media for in vitro culture of Prunus species. Acta Horticultureae, 78: 437-442. - Rong, H.H. 1989. Adventitious root and shoot regeneration in cultured explants of mature black spruce. Plant Sci, 62: 129-135. - Rugini, E. 1984. In vitro propagation of some olive (Olea europaea L. var. sativa) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos. Scientia Horticulture. 24: 123-134. - Schenk, R.U. and Hildebrandt, A.C. 1972. Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Canadian Journal of Botany, 50: 199-204. http://dx.doi.org/10.1139/b72-026 - Shafea, L., Saffari, M., Emam, Y. and Mohammadinejad, G. 2011. Effect of nitrogen and zinc fertilizers on leaf zinc and chlorophyll contents, grain yield and chemical composition of two maize (Zea mays L.) hybrids. Seed and Plant Production Journal. 27-2 (2): 235 - 246. - Sommer, H.E., Brown, C.L. and Kormanik, P.P. 1975. Differentiation of plantlets in longleaf pine (Pinus palustris Mill.) tissue cultured in vitro. Botanical Gazette, 136(2): 196-200. - Taghipoor,M., Haddad, R., and Ghannadnia, M. 2015. The effect of media, explants and cytokinin on micropropagation of Araucaria excelsa R. Journal of Applied Crop Breeding, 3(1): 1-12. - Tang, W., and Newton, R.J. 2007. Micropropagation via organogenesis in slash pine. In Protocols for Micropropagation of Woody Trees and Fruits (pp. 15-22). - Thakur, R.C. and Karnosky, D.F. 2007. Micropropagation and germplasm conservation of Central Park Splendor Chinese elm (Ulmus parvifolia Jacq.‘A/Ross Central Park’) trees. Plant cell reports, 26(8): 1171-1177. - Thomas, M. J., Duhoux, E. and Azart, J.V. 1977. In vitro organ initiation in tissue culture of Biota orientalis and other species of the Cupressaceae. Plant Science Letters, 8: 395-400. | ||
|
آمار تعداد مشاهده مقاله: 442 تعداد دریافت فایل اصل مقاله: 430 |
||