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اثرات سطوح مختلف اسید آمینه والین بر عملکرد، خصوصیات لاشه، کیفیت گوشت و بیان ژن های IGF-1 و انسولین در جوجه‌های گوشتی | ||
| علوم دامی | ||
| مقاله 7، دوره 32، شماره 125، اسفند 1398، صفحه 89-108 اصل مقاله (1.5 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2019.123483.1778 | ||
| نویسندگان | ||
| سیدسعید صادق زاده1؛ محسن دانشیار* 2؛ پرویز فرهومند3؛ محمد رضا یزدیان4؛ سید محمد هاشمی5 | ||
| 1دانشجوی دکتری گروه علوم دامی دانشکده کشاورزی دانشگاه ارومیه | ||
| 2دانشیار گروه علوم دامی دانشگاه ارومیه | ||
| 3استاد گروه علوم دامی دانشگاه ارومیه | ||
| 4استادیار گروه علوم پزشکی دانشگاه آزاد اسلامی واحد قم | ||
| 5استادیار گروه علوم دامی مرکز تحقیقات و کشاورزی استان قم | ||
| چکیده | ||
| این آزمایش به منظور بررسی اثرات سطوح متفاوت والین بر عملکرد، خصوصیات و کیفیت لاشه و بیان ژن‌های فاکتور رشد شبه انسولین (IGF-1) و انسولین در جوجه‌های گوشتی انجام گرفت. آزمایش با 250 قطعه جوجه گوشتی نر یکروزه سویه راس 308 در قالب طرح کاملاً تصادفی، در 5 تیمار و 5 تکرار (در هر تکرار 10 پرنده) با 5 سطح ال-والین (100، 105، 110، 115 و 120 درصد نیازهای سویه راس) انجام شد. در 42 روزگی از هر تکرار یک قطعه جوجه جهت بررسی خصوصیات لاشه، کیفیت لاشه، IGF-1 سرم و بیان ژن‌های IGF-1 و انسولین انتخاب شدند. بهبود افزایش وزن بدن و کاهش ضریب تبدیل با مصرف سطح 110 درصد والین در تمام دوره‌های آغازین، رشد و پایانی مشاهده گردید. مصرف 110 درصد والین موجب افزایش درصد لاشه، سینه، ران و کاهش چربی محوطه بطنی در سن 42 روزگی گردید. بیان ژن‌های IGF-1و انسولین در عضله سینه و بافت کبدی در 42 روزگی با مصرف 115 درصد والین افزایش نشان داد. سطح 110 درصد والین موجب بهبود پروتئین، ماده خشک، چربی و خاکستر گوشت گردید. مصرف سطح 115 درصد والین در 42 روزگی موجب افزایش غلظتIGF-1 سرم شد. به طور کلی، مصرف 10 درصد ال-والین بالاتر از نیازهای سویه راس از راه فعال کردن mTOR در نتیجه فسفوریله شدن S6k1 منجر به آغاز ترجمه mRNA و سنتز پروتئین و در نتیجه موجب افزایش بیان ژن‌های IGF-1 و انسولین، عملکرد و کیفیت لاشه و کاهش درصد چربی محوطه بطنی در جوجه‌های گوشتی نر سویه راس 308 می‌شود. | ||
| کلیدواژه‌ها | ||
| انسولین؛ بیان ژن؛ جوجه‌های گوشتی؛ والین؛ IGF-1 | ||
| عنوان مقاله [English] | ||
| Effects of different levels of valine amino acid on performance, carcas traits, meat quality and insulin like growth factor-1 and insulin genes expression in male Ross 308 broiler chickens | ||
| نویسندگان [English] | ||
| saeid sadeghzadeh1؛ M. Daneshyar2؛ Parviz Farhoomand3؛ M. R. Yazdian4؛ S. M. Hashemi5 | ||
| 1PhD Student, Department of Animal Science, Faculty of Agriculture, University of Urmia I r a n | ||
| 3Department of Animal Science, Urmia University | ||
| چکیده [English] | ||
| The aim of this experimentwas to investiagatethe effects of different levels of valine on performance, carcass traits and quality and insulin like growth factor-1(IGF-1) and insulin genes expression in male Ross 308 broiler ckickens. Present study performed using 250 one day male broiler chicks Ross 308 in completiy randomized design including 25 pen, 5 treatments and 5 replicates each (10 chicks in each replicate) with 5 levels of valine (100, 105, 110, 115, 120 percent of Ross starin requirments). At day 42 of age, one chick from each replicate slaughtered to determine the carcass traits, meat quality, serum IGF-1 and insulin and IGF-1 genes expressions. The improved body weight gain and decreased feed conversion ratio during starter, grower and finisher periods were observed by consumption of 110% valine level. Consumption of 110% valine caused the increased carcass, breast and thigh and lowered abdominal fat at 42 days of age. The IGF-1 and insulin genes expression of breast muscle and liver increased by 115% valine. At day 42 of age, consumption of 110% valine improved the protein, dry matter, crude fat and ash content of meat. In conclusion, consumption of 10% higher than Ross valine requirements increases the IGF-I and Insuline genes expressions through up-reguletion of mTOR and promotion of S6k1 phosphorylation signaling mRNA translettion and protein synthesis and hence improves the performance and carcass quality and decreases the abdominal fat in Ross 308 broiler chickens. | ||
| کلیدواژه‌ها [English] | ||
| Broiler Chicks, Gene Expression, IGF-1, Insulin, Valine | ||
| مراجع | ||
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