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مطالعه اثرات سینرژیستی ترکیبات شبه هورمون پوست‌اندازی و خاک دیاتومه بر قارچ بیمارگر Metarhizium anisopliae برای کنترل لارو سوسک شاخدار خرما Oryctes elegans | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 2، دوره 7، شماره 1 - شماره پیاپی 13، شهریور 1398، صفحه 15-27 اصل مقاله (179.4 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2019.121144 | ||
| نویسندگان | ||
| مسعود لطیفیان* ؛ بهار راد | ||
| پژوهشکده خرما و میوه‌های گرمسیری کشور، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران | ||
| چکیده | ||
| هدف از انجام این پژوهش بررسی اثرات کشندگی و سینرژیستی ترکیبات ضد سنتز کیتین و خاک دیاتومه در کنترل تلفیقی آن‌ها همراه با قارچ Metarhizium anisopliae در جمعیت سوسک شاخدار نخل بود. ترکیبات ضد سنتز کیتینی که در این پژوهش مورد ارزیابی قرار گرفتند شامل آتابرون، دیمیلین و نیمازول بودند. برای بررسی اثر سازگاری ترکیبات با قارچ M. anisopliae، رشد میسلیومی و درصد جوانهزنی قارچ M. anisopliae به روش اختلاط ترکیبات ضد سنتز کیتین با محیط کشت بررسی شد. پس از آن قدرت کشندگی ترکیبات ضد سنتز کیتین سازگار به‌صورت تکی و در تلفیق با قارچ M. anisopliae به‌روش زیست‌سنجی ارزیابی شد. بررسی‌ها نشان داد که قارچ M. anisopliae در تلفیق با کلیه ترکیبات ضد سنتز کیتین توانایی رشد میسلیومی و جوانهزنی اسپور دارد. درصد جوانهزنی اسپور قارچ M. anisopliae در تمام غلظت‌ها در شرایط استفاده از نیمازول بالاتر از سایر ترکیبات بوده است. بالاترین شاخص سازگاری در شرایط اختلاط با غلظت 500 میلی‌لیتر در لیتر نیمازول و کم‌ترین شاخص سازگاری در شرایط اختلاط با غلظت 1000 میلی‌لیتر در لیتر دیمیلین بوده است. هر سه ترکیب دارای اثرات سینرژیستی در قدرت بیمارگری قارچ M. anisopliae روی مرحله رشدی لارو سوسک شاخدار بوده‌اند. اما نیمازول اثرات سینرژیستی بالاتری نشان داد. خاک دیاتومه مورد آزمایش توانایی ایجاد مرگ‌و‌میرروی مرحله رشدی لارو سوسک شاخدار خرما با متوسط غلظت کشندگی معادل 7/1269 میلیگرم بر لیتر را داشت. این ترکیب همچنین دارای اثرات سینرژیستی روی قدرت کشندگی ترکیبات ضد سنتز کیتین و قارچ M. anisopliae بود. اما بیشترین قدرت کشندگی و سینرژیستی در شرایط اختلاط غلظتهای کشنده 50 درصد ترکیبات خاک دیاتومه، نیمازول و قارچ M. anisopliae مشاهده شد. | ||
| کلیدواژه‌ها | ||
| قارچ M. anisopliae؛ ترکیبات ضد سنتز کیتین؛ خاک دیاتومه؛ هم‌افزایی؛ سازگاری | ||
| عنوان مقاله [English] | ||
| Study the synergistic effects of ecdysoids and diatomaceous earth on Metarhizium anisopliae for control of date horned beetle larvae, Oryctes elegans Prell. | ||
| نویسندگان [English] | ||
| Masoud Latifian؛ Bahar Rad | ||
| Date Palm and Tropical Fruits Research Center, Horticulture Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran | ||
| چکیده [English] | ||
| The aim of this study was to evaluate toxic and synergistic effects of ecdysoids compounds and diatomaceous earth on their integrated control along with the entomopathogenic fungus, Metarhizium anisopliae on date palm horned beetle larvae. In this study, the evaluated ecdysoids were Atabron, Dimilinand Nimazol. The fungal mycelium growth and spore germination were evaluated to checking the consistency of the ecdysoids compounds by extract medium mixing method. Then, the toxicity of compounds individually and in conjunction with pathogen M. anisopliae was evaluated by bioassay method. Studies revealed that mycelial growth and spore germination of M. anisopliae occurre in all ecdysoids compounds. The highest compatible index was 76.89 for concentration of 500 microliters per liter of Nimazol extract and the lowest level consistent with the incorporation of Dimilin in 1000 microliters per liter concentration. The combination of ecdysoids compounds with fungal pathogen M. anisopliae had synergistic effect on larval developmental stage of date palm horned beetle. The synergistic effect of Nimazol was higher. The use of diatomaceous earth showed mortality of horned beetle larvae with average lethal concentration equal to 1269.7 mg per liter. It also has synergistic effects on toxicity of fungal pathogen M. anisopliae and anti-synthesis chitin componds. The most powerful synergistic effects were observed in terms of mixing 50 percent lethal concentrations of diatomaceous earth, Nimazole and M. anisopliae. | ||
| کلیدواژه‌ها [English] | ||
| Metarhizium anisopliae, ecdysoid, diatomaceous earth, compatibility, synergism | ||
| مراجع | ||
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