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Effect of Point Mutation in the Growth Differentiation Factor 9 Gene of Oocytes on the Sterility and Fertility of Awassi Sheep | ||
| Archives of Razi Institute | ||
| مقاله 15، دوره 75، شماره 1، خرداد 2020، صفحه 101-108 اصل مقاله (2.56 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.122232.1220 | ||
| نویسندگان | ||
| H. Al-Mutar* 1؛ L. Younis2 | ||
| 1College of Veterinary Medicine, University of Baghdad, Bagdad, Iraq | ||
| 2College of Veterinary Medicine, University of Tikrit, Tikrit, Iraq | ||
| چکیده | ||
| Growth differentiation factor 9 (GDF9) plays a critical role in ovarian follicular development and ovulation rate. The present study aimed to investigate the correlation between the single-nucleotide polymorphism (SNP) of the GDF9 gene and reproductive performance variables, such as fertility and sterility in Awassi sheep. Forty pairs of ovaries from a total of 40 slaughtered Iraqi Awassi ewes were used in this study. Twenty of the ovaries were collected from sterile ewes and the other 20 ovaries were taken from fertile ewes for genomic DNA extraction, polymerase chain reaction, and sequencing to detect GDF9 gene polymorphism. Follicles and oocytes of all the 40 ovaries were evaluated and compared with the results of genotyping. Furthermore, histopathological and microscopic evaluations were performed for 40 ovarian tissues of the two groups. The sequence analysis revealed that exon I had three SNPs, including T(114)C, G(129)R, and G(199)A. The first two SNPs were silent mutations and the last mutation was missense responsible for the substitution of glutamic acid with lysine at position 67. The current study showed a significant increase (P≤0.01) in GG, AA, CC, GA, and GG genotypes at G(129)R, G(199)A, T(114)C, G(129)R, and G(199)A loci, respectively. Moreover, the TT genotype in locus T(114)C was recorded to significantly augment (P≤0.05) in the fertile ewes. Mutant GA genotype of the G(129)R locus led to a significant (P≤0.05) increase in the percentage of follicles (4-8 mm) and oocytes number, compared to wild GG. On the other hand, a significant decrease was recorded in the mutant AA genotype in G(199)A, compared to wild GG. Differences between CC and TT genotypes at T(114)C locus were not significant. Histopathological examination revealed hypoplasia in the ovarian tissue of sterile ewes accompanied by fibrous connective tissue invasion and follicles degeneration. However, in the fertile ewes, the ovarian tissues were normal with the presence of numerous corpus albicans and degenerative corpus luteum. According to the findings of this study, the homozygote mutation in fertile ewes minimized the number of follicles and oocytes leading to sterility, while the heterozygote mutation was reported in the fertile Awassi ewes. | ||
| کلیدواژه‌ها | ||
| Fertility؛ Heterozygote؛ Homozygote؛ Infertility | ||
| عنوان مقاله [English] | ||
| ... | ||
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| مراجع | ||
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Al-Barzinji, Y.M., Othman, G.U., 2013. Genetic polymorphism in FecB gene in Iraqi sheep breeds using RFLP-PCR Technique. IOSR J Agri Vet Sci 2, 46-48.
Asadpour, R.J., Joozani, R.A., 2012. Detection of polymorphism in booroola gene (FecB) and its association with litter size in Zel sheep breed in Iran. Slovak J Anim Sci 45, 63-66.
Bahrami, Y., Bahrami, S., Mohammadi, H.R., Chekani-Azar, V., Mousavizadeh, S.A., 2014. The polymorphism of GDF-9 gene in hisari sheep. Biological Forum Research Trend, p. 46-52.
Barzegari, A., Atashpaz, S., Ghabili, K., Nemati, Z., Rustaei, M., Azarbaijani, R., 2010. Polymorphisms in GDF9 and BMP15 associated with fertility and ovulation rate in Moghani and Ghezel sheep in Iran. Reprod Domest Anim 45, 666-669.
Bodensteiner, K.J., Clay, C.M., Moeller, C.L., Sawyer, H.R., 1999. Molecular cloning of the ovine Growth/Differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries. Biol Reprod 60, 381-386.
Davis, G.H., Balakrishnan, L., Ross, I.K., Wilson, T., Galloway, S.M., Lumsden, B.M., et al., 2006. Investigation of the Booroola (FecB) and Inverdale (FecX(I)) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries. Anim Reprod Sci 92, 87-96.
de Castro, F.C., Cruz, M.H.C., Leal, C.L.V., 2016. Role of growth differentiation factor 9 and bone morphogenetic protein 15 in ovarian function and their importance in mammalian female fertility—A review. Asian Austr J Anim Sci 29, 1065-1074.
Dong, J., Albertini, D.F., Nishimori, K., Kumar, T.R., Lu, N., Matzuk, M.M., 1996. Growth differentiation factor-9 is required during early ovarian folliculogenesis. Nature 383, 531-535.
Elvin, J.A., Clark, A.T., Wang, P., Wolfman, N.M., Matzuk, M.M., 1999. Paracrine actions of growth differentiation factor-9 in the mammalian ovary. Mol Endocrinol 13, 1035-1048.
Elvin, J.A., Yan, C., Matzuk, M.M., 2000. Oocyte-expressed TGF-beta superfamily members in female fertility. Mol Cell Endocrinol 159, 1-5.
Galloway, S.M., McNatty, K.P., Cambridge, L.M., Laitinen, M.P., Juengel, J.L., Jokiranta, T.S., et al., 2000. Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nat Genet 25, 279-283.
Hanrahan, J.P., Gregan, S.M., Mulsant, P., Mullen, M., Davis, G.H., Powell, R., et al., 2004. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol Reprod 70, 900-909.
Juengel, J.L., Hudson, N.L., Heath, D.A., Smith, P., Reader, K.L., Lawrence, S.B., et al., 2002. Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep. Biol Reprod 67, 1777-1789.
Kasiriyan, M.M., Hafezian, S.H., Hassani, N., 2011. Genetic polymorphism BMP15 and GDF9 genes in Sangsari sheep of Iran. Int J Gen Mol Biol 3, 31-34.
Khodabakhshzadeh, R., Mohammadabadi, M.R., Esmailizadeh, A.K., Moradi Shahrebabak, H., Bordbar, F., Ansari Namin, S., 2016. Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Pol J Vet Sci 19, 281-289.
Kumm, K., 2009. Profitable Swedish lamb production by economies of scale. Small Ruminant Res 81, 63-69.
Lafi, S.Q., Talafha, A.Q., Giadinis, N., Kalaitzakis, E., Pourliotis, K., Panousis, N., 2009. Factors affecting the reproductive performance of Awassi sheep flocks in north-east of Jordan: an epidemiological study. Trop Anim Health Prod 41, 1755-1764.
McNatty, K.P., Juengel, J.L., Reader, K.L., Lun, S., Myllymaa, S., Lawrence, S.B., et al., 2005. Bone morphogenetic protein 15 and growth differentiation factor 9 co-operate to regulate granulosa cell function in ruminants. Reproduction 129, 481-487.
Mullen, M.P., Hanrahan, J.P., 2014. Direct evidence on the contribution of a missense mutation in GDF9 to variation in ovulation rate of Finnsheep. PLoS One 9, e95251.
Nicol, L., Bishop, S.C., Pong-Wong, R., Bendixen, C., Holm, L.E., Rhind, S.M., et al., 2009. Homozygosity for a single base-pair mutation in the oocyte-specific GDF9 gene results in sterility in Thoka sheep. Reproduction 138, 921-933.
Notter, D.R., 2008. Genetic aspects of reproduction in sheep. Reprod Domest Anim 43, 122-128.
Ray, A.A., 2012. Statistical analysis system user’s guide: Statistics. Cary, NC: SAS Institute.
Sadighi, M., Bodensteiner, K.J., Beattie, A.E., Galloway, S.M., 2002. Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheep chromosome 5. Anim Genet 33, 244-245.
Souza, C.J., McNeilly, A.S., Benavides, M.V., Melo, E.O., Moraes, J.C., 2014. Mutation in the protease cleavage site of GDF9 increases ovulation rate and litter size in heterozygous ewes and causes infertility in homozygous ewes. Anim Genet 45, 732-739.
Vitt, U.A., Hayashi, M., Klein, C., Hsueh, A.J., 2000. Growth differentiation factor-9 stimulates proliferation but suppresses the follicle-stimulating hormone-induced differentiation of cultured granulosa cells from small antral and preovulatory rat follicles. Biol Reprod 62, 370-377. | ||
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