| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,051 |
| تعداد مقالات | 34,085 |
| تعداد مشاهده مقاله | 46,575,291 |
| تعداد دریافت فایل اصل مقاله | 77,619,107 |
بررسی اثر باسیلوس آمیلولیکوفاسیانس بر کاهش اثرات مضر آفلاتوکسین B1 در جیره جوجه های گوشتی | ||
| علوم دامی | ||
| مقاله 16، دوره 32، شماره 124، آذر 1398، صفحه 213-228 اصل مقاله (1.72 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2018.123443.1776 | ||
| نویسندگان | ||
| منصوره عبدالملکی1؛ علی اصغر ساکی* 2؛ محمد یوسف علیخانی3 | ||
| 1دانشجو دکتری تغذیه طیور دانشکده کشاورزی، دانشگاه بوعلی سینا | ||
| 2استاد گروه علوم دامی دانشکده کشاورزی دانشگاه بوعلی سینا همدان. | ||
| 3استاد گروه میکروب‌شناسی دانشکده پزشکی، دانشگاه علوم‌پزشکی همدان | ||
| چکیده | ||
| در این مطالعه به‌منظور بررسی اثر پروبیوتیک باسیلوس آمیلولیکوفاسیانس در کاهش اثرات مضر آفلاتوکسین B1 از 480 قطعه جوجه گوشتی یک‌روزه (نر و ماده) سویه راس 308 در قالب طرح کاملا تصادفی به‌صورت فاکتوریل (2×2×2) با 8 تیمار، 4 تکرار و تعداد 15 قطعه در هر تکرار استفاده شد. فاکتورها شامل جنسیت (خروس و مرغ)؛ 2) آفلاتوکسین B1 در دو سطح (صفر و 500 میکروگرم در کیلوگرم)؛ 3) پروبیوتیک باسیلوس آمیلولیکوفاسیانس در دو سطح (صفر و 109 واحد تشکیل دهنده کلنی در میلی‌لیتر) بودند. عملکرد، فعالیت آنزیم‌های کبدی، ایمنی و برخی شاخص‌های خونی تحت تاثیر جنسیت قرار نگرفتند (P>0.05). جوجه‌هایی که جیره حاوی آفلاتوکسین B1 خورده بودند، کاهش وزن و مصرف خوراک معنی‌داری نسبت به سایر گروه‌ها داشتند (05/0 p <). ضریب تبدیل غذایی در کل دوره تحت تاثیر تیمارهای آزمایشی قرار نگرفت (05/0P>). فعالیت آنزیم‌های آسپارتات آمینوترانسفراز، آلانین آمینوترانسفراز،آلکالین فسفاتاز و لاکتات دهیدروژناز افزایش معنی‌داری در تیمار آفلاتوکسین B1 نشان دادند (05/0 p <). تعداد گلبول‌های سفید و قرمز و درصد هماتوکریت و هموگلوبین در گروه آفلاتوکسین B1 نسبت به سایر تیمارها به‌طور معنی‌داری کاهش یافتند (05/0 p <). کم‌ترین عیار پادتن علیه بیماری نیوکاسل و گلبول قرمز گوسفند و همچنین کم‌ترین ضخامت پوست در پاسخ به فیتوهماگلوتنین در گروه آفلاتوکسین B1 مشاهده شد(05/0 p <). با توجه به نتایج، مکمل کردن باسیلوس آمیلولیکوفاسیانس به جیره آلوده به آفلاتوکسین B1 توانست عملکرد را بهبود بخشد و فعالیت آنزیم‌های کبدی و پاسخ ایمنی را در مقایسه با جیره آلوده به آفلاتوکسین B1 افزایش دهد (05/0 p <). | ||
| کلیدواژه‌ها | ||
| جوجه‌های گوشتی؛ آفلاتوکسین B1؛ باسیلوس‌آمیلولیکوفاسیانس؛ عملکرد؛ پارامترهای خونی وپاسخ ایمنی | ||
| عنوان مقاله [English] | ||
| Effects of Bacillus amyloliquefaciens on reducing adverse effects of aflatoxin B1 in broilers | ||
| نویسندگان [English] | ||
| Mansoureh Abdolmaleki1؛ Ali asghar Saki2؛ Mohammad yousef Alikhani3 | ||
| 1Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina university | ||
| 2Department of Animal Science, Bu-Ali Sina university, Faculty of Agriculture | ||
| 3Department of microbiology, medical school, university of medical sciences, Hamedan, Iran. | ||
| چکیده [English] | ||
| A study was conducted to evaluate the effects of Bacillus amyloliquefaciens on adverse effects of aflatoxin B1 in broiler chickens. A total of 480 1-d-old broiler Ross 308 (male and female) were completely randomized disgine with a 2× 2 × 2 factorial arrangements (sex and feed additives) with 4 replicates of 15 birds. Factors includes sex (male and female); probiotic (control, 108 cfu/mL B. amyloliquefaciens); aflatoxin (control, 500 AFB1 µg/kg), Performance, the activity of liver enzymes, immune and some blood parameters were not affected by sex (p> 0.05). Body weight and feed intake of aflatoxin B1 group were significantly decreased compared other (p < 0.05). There was no significantly drffenece in feed conversion ratio compared with other groups during total period (p>0.05). Activity of liver enzymes including aspartate amino transferase, alanine amino transferase, alkaline phosphatase and lactate dehydrogenase were significantly increased in aflatoxin B1 group (p < 0.05). The counts of red blood cells and withe blood cells, hematocrite and hemoglobin percentages were significantly decreased in aflatoxin B1 group compared with other (p < 0.05). The aflatoxin B1 group has shown the lowest of antibody production against newcastle disease and sheep blood red cell also, the lowest of skin thickness in responding to phytohaemagglutinin (p < 0.05). In conclusion, the supplementation of B. amyloliquefaciens to contaminated food could be improved performance and increased the activity of liver enzyme and immune response in broilers. | ||
| کلیدواژه‌ها [English] | ||
| broiler chickens, aflatoxin B1, B. amyloliquefaciens, performance, blood parameters and immune | ||
| مراجع | ||
|
Abdel-Wahhab, M.A.; Aly, S.E. (2005). Antioxidant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rats during aflatoxicosis. Journal of Applied Toxicology. 25:218–223. Afzali, N., and Devegowda, G. (2004). The effect of graded levels of dietary aflatoxin on certain biochemical parameters in broiler breeders. WPC 2004, XXII World’s Poultry Congress. Istanbul, Turkey. Ahmed, S.T., Islam, M., Mun, H-S., Sim, H-J., Kim, Y-J., and Yang, C-J. (2014). Effects of Bacillus amyloliquefaciens as a probiotic strain on growth performance, cecal microflora, and fecal noxious gas emissions of broile. chickens. Poultry Science. 93: 1963–1971. Bagherzadeh Kasmani, F.; Karimi Torshizi, M.A.; Allameh, A. and Shariatmadari, F. (2012). A novel aflatoxin-binding Bacillus probiotic: Performance, serum biochemistry, and immunological parameters in Japanese quail. Poultry Science. 91: 1846–1853. Barati, M., Chamani, M., Mousavi, S.N., Hoseiniand, S.A., and Taj Abadi Ebrahimi, M. (2018). Effects of biological and mineral compounds in aflatoxin-contaminated diets on blood parameters and immune response of broiler chickens. Journal of Applied Animal Research. 46 (1): 707–713. Bryden, W.L. (2012). Mycotoxin contamination of the feed supply chain: implications for animal productivity and feed security. Animal Feed Science Technology. 173(1-2): 134-158. Cao, H., Liu, D.L., Mo, X.M., Xie, C.F., and Yao, D.L. (2011). A fungal enzyme with the ability of aflatoxin B 1 conversion: purification and ESI-MS/MS identification. Microbiology Resarch. 166: 475–483. Chang, X., Wu, Z., Wu, S., Dai, Y., and Sun, C. (2015). Degradation of ochratoxin A by Bacillus amyloliquefaciens ASAG1. Food additives and contaminants. Part A, Chemistry, analysis, control, exposure and risk assessment. 32: 564–57. Corcuera, L.A., vettorazzi, A., Arbillaga, L., Gonzalez-Penas, E., and Lopez De Cerain, A. (2012). An approach to the toxity and toxicokinetics of aflatoxin B1 and ochratoxin after simultaneous oral administration to fasted F344 rats. Food Chemistry Toxicology 50: 3440-3446. Corrier, D.E., and Deloach, J.R. (1990). Evaluation of Cell Mediated Cutaneous Basophile Hypersensitivity in Young Chickens by an Interdigital Skin Test. Poultry Science. 69: 403-408. Council for Agricultural Science and Technology (CAST) (2002). Mycotoxins: risks in plant, animal, and human systems. Ames, IA: Council for Agricultural Science andTechnology. Dhanapal, S.K., Rao, S., Govindaraju, P.K.P., Hukkeri, R., and Mathesh, K. (2014). Ameliorative efficacy of citrus fruit oil in aflatoxi-cosis in broilers: A growth and biochemical study. Turkish Journal of Veterinary and Animal Sciences. 38: 207–211. El-Nezami, H., Kankaanpaa, P., Salminen, S. and Ahokas J. (1998). Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media. Journal of Food Protectection. 61:466-468. Elsanhoty, R.M., Salam, S.A., Ramadan, M.F. and Badr, F.H. (2014). Detoxification of aflatoxin M1 in yoghurt using probiotics and lactic acid bacteria. Journal of food control. 43: 129-134. Fan, Y., Zhao, L., Ji, C., Li, X., Jia, R., Xi, L, Zhang, J., and Ma, Q. (2015). Protective Effects of Bacillus subtilis ANSB060 on serum biochemistry, histopathological changes and antioxidant enzyme activities of broilers fed moldy peanut meal naturally contaminated with aflatoxins. Toxins. 7(8):3330-43. Fan, Y., Zhao, L., Ma, Q., Li, X., Shi, H., Zhou, T., zhang, J., and Ji, C. (2013). Effects of Bacillus subtilis ANSB060 on growth performance, meat quality and aflatoxin residues in broilers fed moldy peanut meal naturally contaminated with aflatoxins. Food Chemisrty Toxicology. 59:748-53. Fazeli, M.R., Hajimohammadali, M., Moshkani, A., Samadi, N., Jamalifar, H., and Khoshayand, M.R. (2009). Aflatoxin B1 binding capacity of autochthonous strains of lactic acid bacteria. Journal of Food Protection. 72(1):189–92. Földes, T., Banhegyi, I., Herpai, Z., Varga, L., and Szigeti, J. (2000). Isolation of Bacillus strains from the rhizosphere of cereals and in vitro screening for antagonism against phytopathogenic, food-borne pathogenic and spoilage micro-organisms. Jornal of Applied Microbiology. 89:840 – 846. Fu, X.Q. and Liu, Z.J. (1997). Microhemagglutination inhibition (HI) test. Page 97 in handbook of Poultry Diseases Detection.X.Q. Fu and Z.J. Liu, ed. China Agriculture University Press, Beijingm, China. Gao, X., Ma, Q., Zhao, L., Lei, Y., Shan, Y., and Ji, C. (2011). Isolation of Bacillus subtilis: screening for aflatoxins B1, M1, and G1 detoxification. Eurpean Food Research Technology. 232(6):957-62. Guan, S., Yin, Y., Zhou, T., Xie, M., Ruan, Z., and Young, J.C. (2011). Microbial strategies to control aflatoxins in food and feed. World Mycotoxin Journal. 4:413-424. Huwig, A., Freimund, S., Käppeli, O., and Dutler, H. (2001). Mycotoxin detoxication of animal feed by different adsorbents. Toxicology Letters. 122:179–188. IARC. (2002). Monographs on the evaluation of carcinogenic risks to human. Some traditional herbal medicine, some mycotoxins, naphthalene and styrene. No 82. IARC, Lyon, France Kana, J.R., Ngoula, F., Tchoffo, H., Tadondjou, C.D., Sadjo, Y.R., Teguia, A., and Gbemenou, J. B. (2014). Effect of biocharcoals on hematological, serum biochemical and histological parameters in broiler chickens fed aflatoxin B1-contaminated diets. Journal of Animal Science Advances. 4(7): 939-948 Lawal, M., and Bolu, S. A. (2014). Effects of gallic acid (isolated from grape rind) on serum biochemistry, histology and haematology of Aspergillus flavus challenged broilers. Ethiopian Journal of Environmental Studies and Management. 7: 840–849. Ma, Q.G., Gao, X., Zhou, T., Zhao, L.H., Fan, Y., Li, X.Y, Lie, Y.P., Ji, C., and Zhang, J.Y. Protective effect of Bacillus subtilis ANSB060 on egg quality, biochemical and histopathological changes in layers exposed to aflatoxin B1. Poultry Science. 91(11):2852-7. Mohaghegh, A., Chamania, M., Shivazada, M., Sadeghia, A.A., and Afzalib, N. (2017). Effect of esterified glucomannan on broilers exposed to natural mycotoxin-contaminated diets. Journal of Applied Animimal Resarch. 45:285-291. Mohamed, E.Z., (2011). Impact of mycotoxins on humans and animals. Journal of Saudi Chemistry Society. 15(2): 129-44. Natt, M.P., and Herrick, C.A. (1952). A new diluent for counting the erythrocytes and leukocytes for chickens. Poultry Science. 31: 735-738. Oguz, H.; Keçeci, T.; Birdane, Y.O.; Önder, F.; and Kurtoglu, V. (2000). Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Resarch Veterinary Science. 69:89–93. Petchkongkaew, A., Taillandier, P., Gasaluck, P., and Lebrihi, A. (2008). Isolation of Bacillus spp. from Thai fermented soybean (Thua-nao): screening for aflatoxin B1 and ochratoxin A detoxification. Journal of Applied Microbiology 104(5):1495-502. Peterson, A. L., Qureshi, M. A., Ferket, P. R. and Fuller, J. C. (1999). Enhancement of cellular and humoral immunity in young broilers by the dietary supplementation of β-hydroxy-β-methylbutyrate. Immmunopharmacology and Immunotoxicology. 21 (2): 307-330. Sangare, L., Zhao, Y., Folly, Y.M.E, Chang, J, Li, J., Selvaraj, J.N., Xing, F., Zhou, L., Wang, Y., and Liu, Y. (2014). Aflatoxin B1 degradation by a Pseudomonas strain. Toxins 6(10):3028-40. SAS Institute. (2013) SAS User’s Guide: Statistics. Version 9/2th SAS Institute Inc., Cary, NC. Shotwell, O.L., Hesseltine, C.V., Stubblefield, R.D. and Sorenson, W.G. (1966). Production of aflatoxin on rice. Applied and Environmental of Microbiology. 14: 425-428. Siahmoshteh, F., Siciliano, I., Banani, H., Hamidi-Esfahani, Z., Razzaghi-Abyaneh, M., Gullino ML., and Spadaro, D. (2017). Efficacy of Bacillus subtilis and Bacillus amyloliquefaciens in the control of Aspergillus parasiticus growth and aflatoxins production on pistachio. International Journal of Food Microbiology. 254: 47–53. Stoev, S.D. (2013). Food safety and increasing hazard of mycotoxin occurrence in foods and feeds. Critical Review Food Science and Nutrition. 53(9):887-901. Tung, HT., Cook, F.W., Wyatt, R.D., and Hamilton, P.B. (1975). The anemia caused by aflatoxin. Poultry Science. 54: 1962-1969. Topcu, A., Bulat, T., Wishah, R., and Boyac I.H. (2010). Detoxification of aflatoxin B1 and patulin by Enterococcus faecium strains. International Journal of Food Microbiology. 139: 202–205. Wang, J.S., Luo, H., Billamf, M., Wang, Z., Guan, H., Tang, L., Goldston, T., Afriyie-Gyawu, E., Lovett, C., Griswold, J., Brattin, B., Taylor, R. J., Huebner, H. J., and Phillips, T.D. (2005). Short-term safety evaluation of processed calcium montmorillonite clay (NovaSil) in humans. Food Additives and Contaminants. 22:270-279. Wegmann, T., and Smithies, O. (1966). A simple hemagglutination system requiring small amounts of red cells and antibodies. Transfusion. 6:67-75. Wu, L., Liao, P., He, L.Q., Ren, W., Yin, J, Duan, J., and Li, Tiejun. (2015a). Growth performance, serum biochemical profile, jejunal morphology, and the expression of nutrients transporter genes in deoxynivalenol (DON)-challenged growing pigs. BMC Veterinary Research. 11:144e54. Wu, Li., Liao, Peng., He, L.Q., Feng, Z., Ren, W., Yin, J., Yin, J., Duan, J., Li, T., and Yin, Y. (2015b). Dietary L-Arginine supplementation protects weanling pigs from deoxynivalenol-induced toxicity. Toxins. 7:1341e54. Wu, L., Wang, W., Yao, K., Zhao, T., Yin, J., Li, T.N., Yang, L., He, L.Q., Yang, X.I., Zhang, H.G., Wang, Q., Huang, R., and Yin, Y.G (2013). Effects of dietary arginine and glutamine on alleviating the impairment induced by deoxynivalenol stress and immune relevant cytokines in growing pigs. Plos One. 8 (7): 1-7. Wu, M., Xiao, H., Ren, W., Yin, J., Hu, J., Duan, J., Liu, G., Tan, B., Xiong, X., Oladele, A., Adeola, K.Y., Yin, Y., and Li, T.U. (2014). .An NMR-based metabolomic approach to investigate the effects of supplementation with glutamic acid in piglets challenged with deoxynivalenol. Plos One. 9 (12): 1-15. Xu, J., Wang, H., Zhu, Z., Ji, F., Yin., X, Hong, Q., and Shi, J. (2016). Isolation and characterization of Bacillus amyloliquefaciens ZDS-1: exploring the degradation of zearalenone by Bacillus spp. Food Control. 68: 244–250. Yehia RS. Aflatoxin detoxification by manganese peroxidase purified from Pleurotus ostreatus. Braz J Microbiol 2014;45(1):127e33. Yin, Y., Yan, L., Jiang, J., and Ma, Z. (2008). Biological control of aflatoxin contamination of crops. Journal of Zhejiang University Science. 9(10): 787–92. | ||
|
آمار تعداد مشاهده مقاله: 703 تعداد دریافت فایل اصل مقاله: 461 |
||