| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,845 |
| تعداد مقالات | 32,297 |
| تعداد مشاهده مقاله | 41,382,047 |
| تعداد دریافت فایل اصل مقاله | 18,556,900 |
بررسی اثر تابش دزهای پایین پرتوی گاما به تنهایی و یا همراه با اسید سالیسیلیک و حرارت غیرمستقیم بر آلودگی بذرزاد ویروس موزاییک معمولی لوبیا در گیاه لوبیا | ||
| آفات و بیماری‌های گیاهی | ||
| مقاله 8، دوره 87، شماره 1 - شماره پیاپی 108، شهریور 1398، صفحه 87-105 اصل مقاله (1.13 M) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2019.124059.1251 | ||
| نویسندگان | ||
| فرشاد رخشنده رو* 1؛ حمیده مردانی مهرآباد2؛ سمیرا شهبازی3؛ نوح شهرآئین4 | ||
| 1عضو هیئت علمی و مدیر گروه تخصصی، گروه گیاهپزشکی، دانشکده علوم کشاورزی وصنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2گروه گیاهپزشکی، دانشکده علوم کشاورزی وصنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 3گروه گیاهپزشکی، دانشکده تحقیقات کشاورزی هسته‌ای، موسسه تحقیقات علوم و فناوری هسته‌ای (NSTRI)، سازمان انرژی اتمی ایران (AEOI)، البرز، ایران. | ||
| 4بخش تحقیقات ویروس شناسی، موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| در این پژوهش تأثیر دزهای پایین پرتوی گاما (10، 20 و 30 گری) به‌تنهایی و همچنین همراه با اسید سالیسیلیک و حرارت غیرمستقیم بر ویروس بذرزاد موزاییک معمولی لوبیا (BCMV)، بررسی شد. بذور لوبیای رقم تجاری "صدری" دارای آلودگی بذرزاد به ویروس BCMV کشت شدند. گیاهچههای آلوده و عاری از ویروس بر اساس آزمون الایزای غیرمستقیم (PTA-ELISA) تفکیک و از آنها بذرگیری شد. نتایج نشان داد که شاخصهای رشدی در گیاهچه های آلوده و عاری از ویروس (شاهد) تیمار شده با تابش گاما به تنهایی و نیز همراه با اسید سالیسیلیک و یا حرارت غیرمستقیم در مقایسه با گیاهچههایی که پرتوتابی نشدهاند در سطح احتمال 5 درصد افزایش معنیداری یافته است. پرتوتابی گاما موجب القاء فعالیت اختصاصی آنزیم های آنتیاکسیدان (PPO، POX، CAT و PAL) و همچنین افزایش چگالی پروتئین در گیاهچههای آلوده و سالم و کاهش میزان غلظت پروتئینهای BCMV در گیاهچههای آلوده تیمار شده با پرتو گاما به تنهایی و نیز همراه با اسید سالیسیلیک و یا حرارت غیرمستقیم در مقایسه با گیاهچه های پرتو ندیده شد. نتایج این پژوهش بیانگر القاء مقاومت نسبی در گیاهچه های دارای آلودگی بذرزاد به BCMV تیمار شده با دزهای 20 و 30 گری پرتوی گاما به تنهایی و نیز همراه با اسید سالیسیلیک و حرارت غیرمستقیم بود. | ||
| کلیدواژه‌ها | ||
| اسید سالیسیلیک؛ الگوی پروتئینی؛ پرتوتابی گاما؛ فعالیت آنزیم های آنتی اکسیدان؛ BCMV | ||
| عنوان مقاله [English] | ||
| Study on the effect of low doses of gamma irradiation alone or in associated with the salicylic acid and indirect temperature on seed borne infection of Bean common mosaic virus in bean plant | ||
| نویسندگان [English] | ||
| Farshad Rakhshandehroo1؛ Hamideh Mardani-Mehrabad2؛ samira shahbazi3؛ Nooh Shahraeen4 | ||
| 1College of Agricultural Sciences and Food Technologies , Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 2Department of Plant Protection, College of Agricultural Sciences and Food Technologies , Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 32. Plant Protection Department, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran. | ||
| 4Research Department of Plant Virology, Iranian Research Institute of Plant Protection (IRIPP), Tehran, Iran | ||
| چکیده [English] | ||
| In this study, the effect of the low doses of gamma irradiation (10, 20, and 30 Gy) alone and in associated with the salicylic acid and indirect temperature on the seed borne infection of Bean common mosaic virus (BCMV) was assessed. Commercial bean seeds called “Sadri” with seed borne infection to BCMV were sowed. The seedlings were tested for the infection of BCMV by indirect plate-trapped antigen (PTA- ELISA) test and seeded. Results indicated that the growth factors in the irradiated seeds alone and in associated with the salicylic acid or indirect temperature significantly increased (P≤0.05) in healthy and infected seedlings compared to the non-irradiated seedlings. Gamma irradiation induced the specific activity of various antioxidant enzymes (PPO, POX, CAT and PAL) as well as the total protein density in treated healthy and infected beans, and decreased the concentration of BCMV proteins in the infected and irradiated seeds alone and so in associated with the salicylic acid or indirect temperature compared to the non-irradiated seedlings. Results of this research indicated to the induction of the relative resistance against BCMV in infected bean seedlings treated with the 20 and 30 Gy doses of gamma irradiation alone or in associated with the salicylic acid and indirect temperature. | ||
| کلیدواژه‌ها [English] | ||
| Keywords: Antioxidant enzymes activity, BCMV, gamma irradiation, protein pattern, salicylic acid | ||
| مراجع | ||
|
ALI, H., Z. GHORI, S. SHEIKH, and A. GUL, 2015. Effects of Gamma Radiation on Crop Production. In: Hakeem K. (eds) Crop Production and Global Environmental Issues, Springer, Cham. ARIE, T., H. TAKAHASHI, M. KODAMA, and T. TERAOKA, 2007. Tomato as a model plant for plant-pathogen interactions. Plant Biotechnology, 24:135–147. ANONYMOUS, 2017. Production and yield per hectare of bean crop by province. Agricultural Statistics of the Crop Year 2014-2015. Department of Statistics and Information of Ministry of Agriculture Jihad, 117 pages ARNON, A. N. 1987. Method of extraction of chlorophyll in the plants. AgronomyJournal, 23: 112-121. BAGAL, U. R., J. H.LEEBENS MACK, W. WALTER LORENZ, and J. F. D.DEAN, 2012. The phenylalanine ammonia lyase (PAL) gene family shows a gymnosperm specific line age. BMC Genoms, 13: 1471-2164. BORZOUEI, A., M. KAFI, R. SAYAHI, E. RABIEI, and P. S. AMIN, 2013. Biochemical response of two wheat cultivars (Triticum aestivum L.) to gamma radiation. Pakistanian Journal of Botany, 45: 473–477. BRADFORD, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantites of protein utilizing the principles of protein dyebinding. Analytical Biochemistry, 72: 248-254. CHAKRAVARTY, B. and S. SEN, 2001. Enhancement of regeneration potential and variability by γ-irradiation in cultured cells of Scilla indica. Biologia Plantarum, 44: 189-193. CHANDRA, A., R. SAXENA, A. DUBEY, and P. SAXENA, 2007. Change in phenylalanine ammonia lyase activity and isozyme patterns of polyphenol oxidase and peroxidase by salicylic acid leading to enhance resistance in cowpea against Rhizoctonia solani. Acta Physiologiae Plantarum, 29: 361-367. CHEN, C., R. R. BELANGER, N. BENHAMOU, and T. C. PAULITZ, 2000. Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiological and Molecular Plant Pathology, 56: 13-23. DIAZ-VIVANCOS, P., M. J. CLEMENTE-MORENO, M. RUBIO, E. OLMOS, J. A. GARCIA, P. MARTINEZ-GOMEZ, and J. A HERNANDEZ, 2008. Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to Plum pox virus. Journal of Experimental Botany, 59(8): 2147-2160. DOGAN, S., P. TURAN, and M. DOGAN, 2006. Some kinetic properties of polyphenol oxidase from Thymbra spicata L. var. spicata. Process Biochemistry, 41(12): 2379-2385. DORRI, H.R., A. A. GHANBARI, M. R. LAK, and M. BANI JAMALI, 2008. Guide Bean (Plantation, Cultivation, Harvesting). Educational Technology Services Bureau, Iran (In Farsi). EL-BELTAGI, H., O. K. AHMED, and W. EL-DESOUKY, 2011. Effect of low dose gamma radiation on oxidative stress and secondry metabolites production of resmary (Rosmarinum officinalis L.) callus culture. Radiation Physics and chemistry, 80: 968-976. EL-BOROLLOSY, A. M. and M. M. ORABY, 2012. Induced systemic resistance against Cucumber mosaic cucumovirus and promotion of cucumber growth by some plant growth-promoting rhizobacteria. Annals of Agricultural Science, 57: 91–97. EMAMI, D., J. MEYBODI, and N. MOZAFARI, 2011. Application of electrotherapy for elimination of potato potyviruses, Journal of Agricultural Science and Technology, 13: 921-927. FAO STAT, 2016. National Agricultural statistics Service, USDA.http://www.faostat.fao.org. GONZALEZ, I., L. MARTINEZ, D. RAKITINA, M.G. LEWSEY, F.A. ATIENZO, C. LIAVE, N. KALININA, J.P. CARR, P. PALUKAITIS, and T. CANTO, 2010. Cucumber Mosaic Virus 2b Protein Subcellular Targets and Interactions: Their Significance to its RNA Silencing Suppressor Activity. Molecular Plant-Microbe Interactions, 23: 294-303. HERNANDEZ, J. A., G. GABOR, M. J. Clemente-Moreno, K. ANDRAS, J. CSILLA, and K. LORANT. 2016. Oxidative stress and antioxidative responses in plant-virus interactions. Phsyiological and Molecular Plant Pathology, 94: 134-148. HODGES, M. D., J. M. DE LONG, C. F. FORNEY, and R. K. PRANGE, 1997. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds, Planta, 207: 604-611. HONG, J. S., M. A. JEONG, and R. D. JEONG, 2017. Inhibitory effect of gamma irradiation against Cucumber green mottle mosaic virus. Plant Protection Science, 53: 201-207. HORMOZI-NEJAD, M. H., J. MOZAFARI, and F. RAKHSHANDEHROO, 2010. Elimination of Bean common mosaic virus using an electrotherapy technique. Journal of Plant Diseases and Protection, 117: 201-205. HUMERA, A. 2006. Biochemical and molecular markers of somaclonal variants and induced mutants of potato (Solanum tuberosum L.). PhD Thesis, University of the Punjab Lahore, Pakistan. JAMIL, M., L. DEOGBAE, J. KWANG YONG, M. ASHRAF, L. SHEONG CHUN, R. EUI SHIK, 2006. Effect of salt (NaCl) stress on germination and early seedling growth of four vegetables species. Journal of Central European Agriculture, 7(2): 273-282. JEONG, R. D., E. J. SHIN, E. H. CHU, and H. J. PARK, 2015. Effects of Ionizing Radiation on Postharvest Fungal Pathogens. The Plant Pathology Journal, 31: 176-180. JEONG, M. A. and R. D. JEONG, 2018. Applications of ionizing radiation for the control of postharvest diseases in fresh produce: recent advances. Plant Pathology, 67: 18-29. KIM, D.S., B. K. HWANG, 2014. An important role of the pepper phenylalanine ammonia-lyase gene (PAL1) in salicylic acid-dependent signalling of the defence response to microbial pathogens. Journal of Experimental Botany, 65: 2295-2306. KOVACS, E. and A. KERESZTES, 2002. Effect of gamma and UV-B/C radiation on plant cell. Micron, 33: 199-210. KUHN, C. W. and H. J. TEAS, 1968. Increased resistance to tobacco mosaic virus in gamma irradiated tobacco leaves. Radiation Botany, 317-318. LAEMMLI, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685. LATORRE, M. E., P. NARVAIZ, A. M. ROJAS, and L. N. GERSCHENSON, 2010. Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red Beet (Beta vulgaris L. var. conditiva) root. Journal of Food Engineering, 98: 178-191. LING, A. P. K., J. Y. CHIA, S. HUSSEIN, and A. R. HARUN, 2008. Physiological responses of Citrus sinensis to gamma irradiation. World Applied Sciences Journal, 5: 12-19. LOAKE, G., and M. GRANT, 2007. Salicylic acid in plant defence-the players and protagonists. Current Opinion in Plant Biology, 10: 466-472. LOMMEL, S. A., A. H. MCCAIN, and T. J. MORRIS, 1982. Evaluation of indirect enzyme linked immunoabsorbent assay for the detection of plant viruses. Phytopathology, 72: 1018-1022. MA, X., L. SONG, W. YU, Y. HU, Y. LIU, J. WU, Y. YING. 2015. Growth, physiological, and biochemical responses of Camptotheca acuminata seedlings to different light environments. Frontiers in Plant Science, 6: 321. MAZANDARANI, M. T., E. BEYZAIE, H. R. DORRI, A. A. GHANBARI, P. SALEHI, G. TAHERIYOON, R. MOHAMMADKHANI, N. SHAHRAEEN, and A. H. MEHRABAN, 2010. Shokoufa, a new high yielding white bean cutivar for cultivation in temperate and cold areas of Iran. Seed and Plant Improvement Journal, 26(1): 141-143. MAZANDARANI, M. T., E. BEYZAIE, H. R. DORRI, A. A. GHANBARI, P. SALEHI, G. TAHERIYOON, R. MOHAMMADKHANI, N. SHAHRAEEN, and A. H. MEHRABAN, 2010. Pak, a new high yielding white bean cutivar for cultivation in temperate and cold areas of Iran. Seed and Plant Improvement Journal, 26(1): 145-147. MARCU, D., V. CRISTEA, and L. DARBAN, 2013. Dose-dependent effects of gamma radiation on lettuce (Lactuca sativa var. capitata) seedlings. International Journal of Radiation Biology, 89: 219–223. MARWOOD, C. A. and B. M. GREENBERG, 1996. Effect of supplementary gamma irradiation on chlorophyll synthesis and accumulation of photosystems during chloroplast development in Spirodela oligorrhiza. Photochem, 64: 664- 670. MILONE, M. A., C. SGHERRI, H. CLIJSTERS, and F. NAVARI-IZZO, 2003. Antioxidative responses of wheat treated with realistic concentration of cadmium. Environmental and Experimental Botany, 50: 265-274. MOUSAVI SHELMANI, M. A., B. NASERIAN KHEABANI, H. AHARI MOSTAFAVI, M. HEIDARIEH, and A. MAJD ABADI, 2010. Nuclear Agriculture. Nuclear Sciences and Technology Research Institute Press, Tehran, Iran. NADERPUR, M., GH. MOSAHEBI MOHAMMADI, and M. KOHI, 2000. Pathotype of Vta III, Vib and necrotic strains NL-3, NL- 8, NL-5, and virus BCMNV new report on Iran. The Proceeding of 14th Plant Protection Congress, Isfahan, Iran. NANDEESHKUMAR, P., B. SAROSH, K. R. KINI, H. PRAKASH, and H. S. SHETTY, 2009. Elicitation of resistance and defense related proteins by β-amino butyric acid in sunflower against downy mildew pathogen Plasmopara halstedii. Archives of Phytopathology and Plant Protection, 42: 1020-1032. NASERIAN, K., M. AHARI, H. FATHOLLAHI, S. VEDADI, and S. MOSAVI, 2008. Suitable gamma ray dose determination in order to induce genetic variation in kaboli chickpea (Cicer Arietinum L). Journal of Nuclear Science and Technology, pp. 19-25. PEYAMBARI, M., M. KOOHI HABIBI, GH. MOSAHEBI, and K. IZADPANAH, 2011. Study of bean common mosaic virus (BCMV) in several provinces and reaction of three bean genotypes to BCMV infection. Journal of Plant Protection, 25: 250-257. PIRI, I., M. BABAYAN, A. TAVASSOLI, and M. JAVAHERI, 2011. The use of gamma irradiation in agriculture. African Journal of Microbiology Research, 5 (32): 5806-5811. POLESSKAYA, O. G., E. I. KASHIRINA, and N. D. ALEKHINA, 2004. Changes in the activity of antioxidant enzymes in wheat leaves and roots as a function of nitrogen source and supply. Russian Journal of Plant Physiology, 51: 615-620. PREDMORE, A., G. C. SANGLAY, E. DICAPRIO, J. LI, R. M. URIBE, and K. LEE, 2015. International Journal of Food Microbiology, 198: 28-36. RADWAN, D. E., G. LU, K. A. FAYEZ, and S.Y. MAHMOUD, 2008. Protective action of salicylic acid against bean yellow mosaic virus infection in Vicia faba leaves. Journal of Plant Physiology, 165: 845-857. REDDICK, D. and U. B. STEWARD, 1918. Varieties of bean susceptible to mosaic. Phytopathology, 8:530-534. ROGOZHIN, V. V., T.T. KURILIUK, and N. P. FILIPPOVA, 2000. Change in the reaction of the antioxidant system of wheat sprouts after UV-irradiation of seeds. Biofizika, 45: 730-736. SALARI, N., M. SEYYED MUSAVI, N. SHAHRAEEN, SH. GHORBANI, and M. MALEKI, 2013. Identification and diagnosis of isolates of bean common mosaic virus and bean common mosaic necrosis virus by immunocapture RT-PCR. New Cellular and Molecular Biotecnology Journal, 3(11): 21-28. SGHERRI, C. L. M., M. MAFFEI, and F. NAVARI-IZZO, 2000. Antioxidant enzymes in wheat subjected to increasing water deficit and rewatering. Journal of Plant Physiology, 157: 273-279. SHAHBAZI, S. 2016, Increasing the tolerance of sugar beet and soybean seedlings to Rhizoctonia solani contamination by irradiation of seeds with gamma rays in order to reduce seedling death. Project Code: PRN-A3-95-003. SMOLKO, E. E. and J. H. LOMBARDO, 2005. Nuclear Instruments and Methods in Physics Research, 236: 249-253. STAJNER, D., M. MILOSEVIC, and B. M. POPOVIC, 2007. Irradiation effects on phenolic content, lipid and protein oxidation and scavenger ability of Soybean seeds. International Journal of Molecular Sciences, 8: 618-627. STRAUSBAUGH, C. A., P. N. MIKLAS, S. P. SINGH, J. R. MYERS, and R. L FORSTER, 2003. Genetic characterization of differential reaction among host group 3 common bean cultivars to NL-3 k strain of Bean common mosaic necrosis virus. Phytopathology, 93:683-690. TURKAN, I., M. BOR, F. OZDEMIR, and H. KOCA, 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought - tolerant P. acutifolius gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science, 168: 223-231. UMESHA, S. 2006. Note: Phenylalanine ammonia lyase activity in tomato seedlings and its relationship to bacterial canker disease resistance. Phytoparasitica, 34: 68-71. VARIYAR, P. S., A. LIMAYE, and A. SHARMA, 2004. Radiation-induced enhancement of antioxidant contents of soybean (Glycine max Merrill). Journal of Agricultural and Food Chemistry, 52: 3385–3388. WI, S. G., B. Y. CHUNG, J. S. KIM, J. H. KIM, M. H. BAEK, and J. W. LEE, 2006. Localization of hydrogen peroxide in pumpkin (Cucurbita ficifolia bouché) seedlings exposed to high-dose gamma ray. Journal of Plant Biology, 49: 1-8. WI, S. G., B. Y. CHUNG, and J. S. KIM. 2007. Effects of gamma irradiation on morphological changes and biological responses in plants. Micron, 38: 553–564. ZHENG, T., A. M. NAPIER, J. P. PARKES, J. S. O’KEEFE, and P. H. ATKINSON, 2002. Detection of RNA of rabbit haemorrhagic disease virus from New Zealand wild rabbits. Wildlife Research, 29: 683 - 688.
| ||
|
آمار تعداد مشاهده مقاله: 577 تعداد دریافت فایل اصل مقاله: 372 |
||