اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,907
تعداد مقالات32,799
تعداد مشاهده مقاله43,068,140
تعداد دریافت فایل اصل مقاله19,658,734
Mesa, Ana María, Blair, Silvia, Pelaez, Carlos. (1398). Chemical Standardization of Extracts of CalophyllumBrasiliense with Antiplasmodial and Cytotoxic Activity. سامانه مدیریت نشریات علمی, 8(2), 171-179. doi: 10.22092/jmpb.2019.120495
Ana María Mesa; Silvia Blair; Carlos Pelaez. "Chemical Standardization of Extracts of CalophyllumBrasiliense with Antiplasmodial and Cytotoxic Activity". سامانه مدیریت نشریات علمی, 8, 2, 1398, 171-179. doi: 10.22092/jmpb.2019.120495
Mesa, Ana María, Blair, Silvia, Pelaez, Carlos. (1398). 'Chemical Standardization of Extracts of CalophyllumBrasiliense with Antiplasmodial and Cytotoxic Activity', سامانه مدیریت نشریات علمی, 8(2), pp. 171-179. doi: 10.22092/jmpb.2019.120495
Mesa, Ana María, Blair, Silvia, Pelaez, Carlos. Chemical Standardization of Extracts of CalophyllumBrasiliense with Antiplasmodial and Cytotoxic Activity. سامانه مدیریت نشریات علمی, 1398; 8(2): 171-179. doi: 10.22092/jmpb.2019.120495

Chemical Standardization of Extracts of CalophyllumBrasiliense with Antiplasmodial and Cytotoxic Activity

Journal of Medicinal plants and By-Products
مقاله 8، دوره 8، شماره 2، آذر 2019، صفحه 171-179    اصل مقاله (198.04 K)
شناسه دیجیتال (DOI): 10.22092/jmpb.2019.120495
نویسندگان
Ana María Mesa* 1؛ Silvia Blair2؛ Carlos Pelaez3
1Agrobiotechnology Research Group, Institute of Biology, Faculty of Exact and Natural Sciences, University of Antioquia, Medellín. Colombia
2Malaria Research Group. University Research Headquarters (SIU). Carrera 62 52-59. Tower 1.Lab. 610 SIU. Faculty of Medicine. University of Antioquia. A. A 1226.Medellín, Colombia
3Interdisciplinary group of molecular studies GIEM. Faculty of Exact and Natural Sciences, University of Antioquia. A. A 1226. Medellín, Colombia
چکیده
Malaria is considered one of the health problems in many parts of the world. The objective of this work is to implement standardized extraction strategies using analytical methodologies of the Calophyllum brasiliense Cambess. for the validation of antiplasmodial potential in vitro. Using HPLC chromatographic and chemical characterization techniques (13C-NMR, 1H-NMR), the marker substance was identified and quantified on continuous cultures of Plasmodium falciparum chloroquine-sensitive strain NF-54 and cytotoxicity in cell lines U-937 and HepG2used the antimalarial potential of the C. brasiliense. The best extracts were stems with ethyl acetate and extraction after 4 hours, presented a yield of 4.154±0.301%, a promising antiplasmodial activity of IC50 = 9.013 ± 1.288 μg/mL, a cytotoxicity of CC50HepG2 =43.340±3.159 μg/mL, an IS = 4.808 and a content of marker substancesnamedsoulatrolide (1)of C1 =0.257±0.021%. A new analytical method for the quantification of marker substances was established and the best extracts were obtained. The extracts presented promising pharmacological activities for studies that guarantee reproducibility and effectiveness in biological tests in vivo and framed in the transformation of a phytotherapeutic product.
کلیدواژه‌ها
Antiplasmodialactivity؛ Cytotoxic activity؛ Phytomedicine؛ Plasmodium falciparum؛ Standardized extract
مراجع
1. WHO, 2015. World Malaria Report 2014. Geneva, World Health Organization. 2014. ( Internet: http://www.who.int/malaria/publications/world_malaria_report_2014/en/ ).
2. OMS, 2017. World Technical Strategy against Malaria 2016-2030. World Health Organization. 2016. http://www.who.int/malaria/publications/atoz/9789241564991/es/ consultado el 16 de Junio de. 2017.
3. World Malaria Report 2018. Geneva: World Health Organization. 2018. Licence: CC BY-NC-SA 3.0 IGO (Internet: https://www.who.int/malaria/publications/world-malaria-report-2018/report/en/)
4. Menard D, Dondorp A. Antimalarial Drug Resistance: A Threat to Malaria Elimination. Cold Spring HarbPerspectMedJuly. 2017;7. doi:10.1101/cshperspect.a025619.
5. Grimberg BT, Mehlotra RK. Expanding the Antimalarial Drug Arsenal-Now, But How?.Pharmaceuticals (Basel). 2011;4:681-712.
6. Chin J. The control of comunicable diseases 17th edition. Editor Official report of the American Public Health Association Seventeenth edition. Pan American HealthOrg. 2001;748.
7. Olumese P. Guidelines for the treatment of malaria Third edition. World Health Organization. 313.
8. Newman DJ, Cragg GM. Natural products as sources of new drugsoverthe 30 yearsfrom 1981 to 2010. J Natural Products. 2012;75:311-335.
9. Noldin VF, Isaias F, Cechinel V. Calophyllum Genus: Chemical and pharmacological importance. Quim. Nova. 2006;29:549-554.
10. Kimura S, Ito C, Jyoko N, Segawa H, Kuroda J, Okada M, Adachi S, Nakahata T, Yuasa T, Filho VC, Furukawa H. Maekawa T. Inhibition of leukemic cell growth by a novel anti‐cancer drug (GUT‐70) from calophyllum brasiliense that acts by induction of apoptosis. Int J Cancer. 2005;113:158-165.
11. Kouhestani F, Dayer MS, Kamali H. Reversed-phase Liquid Chromatographic Quantification of Pyrethrins in the Extract of Wild Tanacetum parthenium (Feverfew) from Northern Khorasan Province (Iran). 2013
12. Hout S, Chea A, Bun SS, Elias R, Gasquet M, Timon-David P, Balansard G, Azas N. Screening of selected indigenous plants of Cambodia for antiplasmodial activity. J Ethnophar. 2006;107:12-18.
13. Mesa Vanegas, AM. Estudio fitoquímico de algunas especies de género Piper y Calophyllum con actividad antiplasmodial, citotóxica y antioxidante. estandarización química y estudios de relación estructura-actividad (SAR). Tesis doctoral, Universidad de Antioquia, Medellín, Colombia. 2013.
14. Smilkstein M, Sriwilaijaroen N, Kelly JX, Wilairat P, Riscoe M. Simple and Inexpensive Fluorescence-Based Techniquefor High-Throughput Antimalarial Drug Screening. Antimicrobial Agents and Chemotherapy. 2004;48:1803-1806.
15. Malebo MH, Tanja W, Cal M, Swaleh SAM, Omolo MO, Hassanali A. Séquin U, Hamburger M, Brun R, Ndiege IO. Antiplasmodial, anti-trypanosomal, anti-leishmanial and cytotoxicity activity of selected Tanzanian medicinal plants. Tanzania J Health Res. 2009;11:47-56.
16. Deharo E. A search for natural bioactive compounds in Bolivia through a multidisciplinary approach Part II. Antimalarial activity of some plants used by Mosetene Indians. J Ethnopharm. 2000;69:139-155.  
17. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunological Methods. 1983;65:55-63.
18. Moore GE, Gerner RE, Franklin HA. Culture of normal human leukocytes. Jama. 1967;199:519-524.
19. Reed GF, Lynn F, Meade BD. Use of coefficient of variation in assessing variability of quantitative assays. Clin Diagn Lab Immunol. 2002;9:1235-1239.
20. Kaou AM, Mahiou-Leddet V, Hutter S, Ainouddine S, Hassani S, Yahaya I. Antimalarial activity of crude extracts fromnine African medicinal plants. J Ethnopharmacol. 2008;116:74-83.
21. Albernaz LC, De Paula JE, Romero GA, Silva Mdo R, Grellier P, Mambu L, Espindola LS. Investigation of plant extracts in traditional medicine of the Brazilian Cerrado against protozoans and yeasts. J Ethnopharm. 2010;131:116-121.
22. Brenzan MA, Piloto Ferreira IC, Campana Lonardoni, MV, Honda PA, Filho ER, Nakamura CV, Garcia Cortez DA. Activity of extracts and coumarins from the leaves of Calophyllum brasiliense. On leishmania braziliensis. Pharmace Biology. 2008;46:380-386.
23. Ito C, Itoigawa M, Mishina Y, Cechinel-filho V, Enjo F, Tokuda H, Nishino H, Furukawa H. Chemical constituents of Calophyllum brasiliense. 2: Structure of three new coumarins and cáncer chemopreventive activity of 4-substituted coumarins. J Nat Prod. 2003;66:368-375.
24. Kashman Y, Gustafson KR, Fuller RW, Cardellina JH, Mcmahon JB, Currens MJ, Buckheit RW, Jr., Hughes SH, Cragg GM, Boyd MR. HIV inhibitory natural products. Part 7. The calanolides, a novel HIV-inhibitory class of coumarin derivatives from the tropical rainforest tree, Calophyllum lanigerum. J Med Chem. 1992;35:2735-2743.
25. Itoigawa M, Ito C, Tan HTW, Kuchide M, Tokuda H, Nishino H, Furukawa H. Cancer chemopreventive activity of naphthoquinones and their analogs from Avicennia plants. Cancer Lett. 2001;169:15-20.
26. Kijjoa A, Gonzalez MJ, Pinto MM, Anantachoke C, Herz W. Xanthones from Calophyllum teysmannii var. inophylloide. Phytochem. 2000;55:833-837.
27. Barth A, Müller D, Dürrling K. In vitro investigation of a standardized dried extract of Citrullus Colocynthis Onlivertoxicity in adult rats. Exp Toxic Pathol. 2002;54:223-230.
28. Novak N, Haberstok J, Kraft S, Siekmann L, Allam JP, Bieber T. Standardized extracts from Chinese herbs induce IL-10 production in human monocyte-derived dendritic cells and alter their differentiation in vitro. J Allergy Clin Immunol. 2001;108:588-593.
29. Fidock DA, Rosenthal PJ, Croft SL, Brun R, Nwaka S. Antimalarial Drug Discovery: efficacy models for compound screening. Nature Reviews. Drug Discovery. 2004;3:509-520.
30. Jachak SM, Gautam R, Selvam C, Madhan H, Srivastava A, Khan T. Anti-inflammatory, cyclooxygenase inhibitory and antioxidant activities of standardized extracts of Tridax procumbens L. Fitoterapia. 2011;82:173-177.
31. Tanaea MM, Lima-Landman MTR, De Lima TCM, Souccar C, Lapa AJ. Chemical ‎Standardization ‎theaqueousextract ‎Cecropiaglaziovii Snethendowedwith ‎antiacidsecretion ‎likeactivities. ‎Phytomedicine. 2007;14: 309-313.

آمار
تعداد مشاهده مقاله: 513
تعداد دریافت فایل اصل مقاله: 379


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب