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Taxonomic study of cyanoprokaryotes from medicinal plants bed with emphasis on phylogeny of complex taxa using 16S rRNA marker | ||
| Rostaniha | ||
| مقاله 2، دوره 20، شماره 2 - شماره پیاپی 58، اسفند 2019، صفحه 98-109 اصل مقاله (483.51 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/botany.2019.126659.1159 | ||
| نویسندگان | ||
| Somayeh Zarezadeh1؛ Hossein Riahi2؛ Zeinab Shariatmadari* 3؛ Masoumeh Sadat Hosseini4 | ||
| 1PhD Student, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran | ||
| 2Prof., Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran | ||
| 3Assistant Prof., Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran | ||
| 4MSc Graduate, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran | ||
| چکیده | ||
| Cyanoprokaryotes are simple photosynthetic microorganisms which have an important role in the soil carbon and nitrogen cycle. The current study aimed to investigate the flora of cyanoprokaryotes from medicinal plants bed. Also phylogenetic analysis based on 16S rRNA marker was performed to investigate the phylogenetic relationships between different cyanoprokaryotic taxa and evaluate the efficiency of this marker in separation of taxonomic boundaries between taxa especially in the case of complex taxa, which their relations are not well-defined. For this purpose, after collection of soil, isolation and purification of strains were performed. The cyanoprokaryotic taxa were identified morphologically and 16S rRNA marker was used to approve the identifications. Phylogenetic analysis performed using Maximum Likelihood, Maximum Parsimony and Bayesian Inference. Totally, 42 cyanoprokaryotic taxa were identified and Nostoc was an abundant genus in the soil of medicinal plants bed. The phylogenetic tree revealed Nostocales as a monophyletic group. Also, Wollea together with Anabaena, and Nostoc together with Desmonostoc created monophyletic groups. Results revealed that, 16S rRNA is an effective phylogenetic marker in high classification rankings such as order, family and genus. However, 16S rRNA could not be an effective marker in separation of close genera such as Nostoc and Desmonostoc. | ||
| کلیدواژه‌ها | ||
| Cyanoprokaryotes؛ Desmonostoc؛ phylogeny؛ Wollea؛ 16S rRNA | ||
| عنوان مقاله [English] | ||
| مطالعه تاکسونومیک سیانوپروکاریوت‌های بستر رویشی گیاهان دارویی با تاکید بر فیلوژنی آرایه‌های پیچیده با استفاده از مارکر 16S rRNA | ||
| نویسندگان [English] | ||
| سمیه زارع زاده1؛ حسین ریاحی2؛ زینب شریعتمداری3؛ معصومه سادات حسینی4 | ||
| 1دانشجوی دکتری دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران | ||
| 2استاد دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران | ||
| 3استادیار دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران | ||
| 4فارغ‌التحصیل کارشناسی ارشد، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران | ||
| چکیده [English] | ||
| سیانوپروکاریوت‌ها موجودات ریز و ساده فتوسنتز‌کننده‌ای هستند که نقش مهمی در چرخه‌های نیتروژن و کربن خاک ایفا می‌نمایند. هدف مطالعه حاضر، بررسی فلور سیانوپروکاریوت‌های موجود در بستر رویشی گیاهان دارویی است. همچنین، بررسی فیلوژنتیک به منظور ارزیابی روابط فیلوژنی میان آرایه‌های مختلف سیانوپروکاریوتی با استفاده از مارکر 16S rRNA و ارزیابی کارآمدی این مارکر در جداسازی مرزهای تاکسونومیک میان آرایه‌ها، به ویژه در موارد پیچیده که روابط میان آن‌ها به خوبی شناخته شده نیست، انجام گرفته است. به این منظور، پس از جمع‌آوری خاک، جداسازی و خالص‌سازی سویه‌ها انجام گرفت. آرایه‌های سیانوپروکاریوتی براساس خصوصیات ریخت‌شناختی شناسایی شدند و تایید شناسایی‌ها با کمک مارکر مولکولی 16S rRNA انجام گرفت. روابط فیلوژنی با استفاده از Maximum Likelihood، Maximum Parsimony و Bayesian Inference مورد ارزیابی قرار گرفت. در مجموع، 42 آرایه سیانوپروکاریوتی شناسایی شد و نتایج نشان‌دهنده غالب بودن جنس گونه Nostocدر فلور بستر رویشی گیاهان دارویی بود. درخت حاصل از نتایج فیلوژنی نشان‌دهنده تک‌نیایی بودن Nostocales است. همچنین، جنس‌های Wollea و Anabaena با یکدیگر و نیز جنس‌هایNostoc و Desmonostoc با هم، گروه‌های تک‌نیا را تشکیل دادند. نتایج نشان‌دهنده کارآمدی مارکر16S rRNA در سطوح بالای سیستم رده‌بندی، اعم از راسته، تیره و جنس می‌باشد. با این وجود، این مارکر در جداسازی آرایه‌های نزدیک به یکدیگر نظیر Desmonostoc و Nostoc از کارآمدی لازم برخوردار نیست. | ||
| کلیدواژه‌ها [English] | ||
| سیانوپروکاریوت, 16S rRNA, فیلوژنی, Wollea, Desmonostoc | ||
| مراجع | ||
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