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Bradykinin-Potentiating Factors of Venom from Iranian Medically Important Scorpions | ||
| Archives of Razi Institute | ||
| مقاله 8، دوره 74، شماره 4، اسفند 2019، صفحه 385-394 اصل مقاله (700.9 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.123404.1249 | ||
| نویسندگان | ||
| H. R. Goudarzi* 1؛ Z. Salehi Najafabadi2؛ A. Movahedi1؛ M. Noofeli2 | ||
| 1Central Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 2Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| The venom of animals, including snakes, scorpions, and spiders is a complex combination of proteins, peptides, and other biomolecules as well as some minerals. Among the biomolecules of some animal’s venom, small peptides that lack disulfide bands known as Non-Disulfide Bridge Peptides (NDBPs) potentiate the bradykinin by preventing the conversion of angiotensin 1 to angiotensin 2 using the mechanism of inhibiting the Angiotensin-Converting Enzyme activity and finally reducing the blood pressure in the victims. This feature of the NDBPs of animal’s venom is suggested as the potential of biological drugs. This study aimed to isolate venom components of three species of Iranian medically important scorpions and study the bradykinin potentiating effect of them. The scorpion specimens were collected from the venomous animals and antivenom production department of Razi Vaccine and Serum Research Institute, Karaj, Iran. Moreover, venom extraction was performed by electrical shock (5 volts). The obtained liquid venom of three species specimens was frozen and lyophilized immediately and then preserved in a cool and dried place. The isolation of the venom components for each scorpion was carried out using high-performance liquid chromatography. The obtained ranges of venom fractions (zones) were tested on isolated tissues of guinea-pig ileum and rat uterus using organ bath instrumentation in several replicates. The bioassays resulted in the peptides, including Z1 and Z2 regions in the venom fractionsof the Hottentotta saulcyi, Z2 in Odontobuthus doriae, as well as Z2 and Z3 in Mesobuthus eupeus demonstrated bradykinin potentiating effect. It is concluded that Bradykinin Potentiating Factors were traceable in the venom of all three scorpion species. Therefore, these venoms have the therapeutic potential to exploit biological-based drugs. | ||
| کلیدواژه‌ها | ||
| Biologic drugs؛ Bradykinin-potentiating factors؛ Hypertension؛ Scorpion venom | ||
| عنوان مقاله [English] | ||
| فاکتورهای تقویت کننده برادیکینین در زهر عقربهای دارای اهمیت پزشکی ایران | ||
| چکیده [English] | ||
| زهر جانوران اعم از مارها، عقربها، عنکبوتها و... ترکیب پیچیدهای متشکل از انواع پروتئینها ، پپتیدها ودیگر ملکولهای حیاتی و نیز برخی مواد معدنی است. از جمله ملکولهای زیستی موجود در زهر جانوران سمی پپتیدهایی هستند که طی مکانیسم بازدارندگی از تبدیل آنژیوتنسین 1 به آننژیوتنسین 2 موجب کاهش فشار خون در فرد مورد گزش میشوند. هدف مطالعه حاضر جداسازی اجزاء زهر ۳ گونه از عقربهای دارای اهمیت پزشکی ایران و زیست سنجی آنها برای بررسی اثر تقویت کنندگی برادیکینین بود. در این بررسی ابتدا زهر عقربهای دارای اهمیت پزشکی ایران بوسیله دستگاه الکتروشوک استحصال و سپس لیوفلیزه گردید. جداسازی اجزاء زهر هریک از عقربها با استفاده از روش کروماتوگرافی با عملکرد بالا (HPLC) انجام شد. محدودههای فراکسیونهای (Zones) به دست آمده در چندین تکرار بر روی بافت ایلئوم خوکچه هندی (Guinea pig Ileum) و بافت لوله رحم موش صحرایی (Rat Uterus) با استفاده از دستگاه Organ Bath انجام شد. نتایج نشان داد که پپتیدهای نواحی Z1 و Z2 فراکسیونهای زهر عقرب Hottentotta sulcyi ، ناحیه Z2 برای زهر عقرب Odontobutus doriea و پپتیدهای نواحی Z2 و Z3 در زهر عقرب Mesobutus eupeus دارای اثر تقویت کنندگی برادیکینین هستند. | ||
| کلیدواژه‌ها [English] | ||
| زهر عقرب, فاکتور تقویت کننده برادیکینین, فشار خون, داروهای بیولوژیک | ||
| مراجع | ||
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