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Efficacy of CpG-ODN Administration Routes on Humoral Responses against Newcastle disease in Broilers | ||
| Archives of Razi Institute | ||
| مقاله 5، دوره 74، شماره 4، اسفند 2019، صفحه 357-364 اصل مقاله (292.42 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.120544.1196 | ||
| نویسندگان | ||
| A. Talebi* 1؛ S. Arky-rezai2 | ||
| 1Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| 2Graduated, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| چکیده | ||
| Un-methylated cytosine-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) has been considered as a powerful vaccine adjuvant and recognition of CpG-ODN by chicken leukocytes promotes their ability to fight against infections. In our study, efficacy of different routes of CpG-ODN application as an adjuvant on immune responses (antibody titer together with leukogram) following vaccination against Newcastle disease (ND) has been evaluated in broiler chickens (Ross-308). The results indicated that routes of CpG-ODN administration influence immune responses and comparison effectiveness of CpG-OND delivery routes showed that group vaccinated by eye-drop application had the highest antibody titer than that of the group injected intramuscularly (im) and the difference was significant (p = 0.04) on day 35 of age. Antibody titer of the group treated with Clone 30 plus CpG-ODN via eye-drop route was higher than that of the group vaccinated with clone 30 alone on days 28 and 35 of age and the difference was significant (p = 0.04). Co-administration of both vaccine and CpG improved outcome of leukogram of the chickens on days 21 to 42 of age and among the treated groups, WBC of the group received both vaccine and CpG by eye-drop route significantly (p < 0.05) differed from that of the group vaccinated with clone 30 alone on days 28 and 35 but not on day 42 of age. Average final body weight of the control group did not significantly differ from those of the treated groups at end of the experiment. In conclusion, co-administration of ND vaccine plus CpG-ODN via eye-drop route improves immune responses. | ||
| کلیدواژه‌ها | ||
| CpG؛ Antibody titer؛ ND؛ Broilers؛ Vaccine administration route | ||
| عنوان مقاله [English] | ||
| مطالعه تاثیر روشهای تجویز CpG-ODN بر روی پاسخ همورال بر علیه بیماری نیوکاسل در طیور گوشتی | ||
| چکیده [English] | ||
| CpG-ODN به عنوان یک یاور توانمند واکسنها در نظر گرفته میشود که شناسایی آن بوسیله لکوسیتهای طیور منجر به بهبود قدرت آنها در دفاع با عفونتها میگردد. در مطالعه ما، تاثیر روشهای مختلف تجویز CpG-ODN بههمراه واکسن زنده بر پاسخهای ایمنی (تیتر آنتیبادی و لکوگرام) ناشی از واکسیناسیون بر علیه بیماری نیوکاسل بصورت هفتگی در طیور گوشتی راس 308 ارزیابی گردید. نتایج این مطالعه بیانگر آن است که روشهای تجویز CpG-ODN، پاسخهای ایمنی را تحت تاثیر قرار داده و روش قطره در مقایسه با روش تزریقی بیشترین (p = 0.04) تاثیر را در سن 35 روزگی دارد. تیتر آنتیبادی گروه واکسینه شده با واکسن کلون 30 بههمراه CpG-ODN بهطریقه قطره چشمی نیز در مقایسه با تجویز واکسن تنها و سایر روشهای تجویز واکسن به همراه CpG-ODNدر سنین 28 و 35 روزگی آزمایش بالاتر (p = 0.04) بود. تجویز همزمان واکسن بههمراه CpG، لکوگرام طیور را در سنین 28 الی 42 روزگی بهبود بخشید و در بین روشهای تجویز، فقط تجویز همزمان واکسن بههمراه CpG با روش قطره چشمی در سنین 28 و 35 روزگی در مقایسه با طیور واکسینه شده با واکسن تنها، به صورت معنیداری (p < 0.05) افزایش یافت. در انتهای دوره آزمایش، تفاوت معنیداری در وزن نهایی پرندگان مشاهده نگردید. نتیجهگیری اینکه تجویز همزمان CpG-ODN بههمراه واکسن زنده نیوکاسل بهطریقه قطره چشمی پاسخهای ایمنیهمورال را بهبود میبخشد. | ||
| کلیدواژه‌ها [English] | ||
| CpG-ODN, تیتر آنتیبادی, نیوکاسل, طیور گوشتی, روش تجویز واکسن | ||
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