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بررسی آزمایشگاهی اثرات وابسته به دوز آنتی‌بیوتیک سالینومایسین بر تخمیر شکمبه و برخی از جمعیت‌های میکروبی شکمبه با استفاده از روش Real-time PCR | ||
| علوم دامی | ||
| مقاله 13، دوره 32، شماره 123، شهریور 1398، صفحه 167-182 اصل مقاله (3.12 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2018.122848.1744 | ||
| نویسندگان | ||
| نسیبه نویدی مقدم1؛ مصطفی ملکی* 2؛ اصغر میرزائی اصل3 | ||
| 1دانش آموخته کارشناسی ارشد گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه بوعلی سینا، همدان | ||
| 2استادیار گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه بوعلی سینا، همدان | ||
| 3دانشیار گروه بیوتکنولوژی، دانشکدۀ کشاورزی، دانشگاه بوعلی سینا، همدان | ||
| چکیده | ||
| تحقیق حاضر به‌منظور بررسی آزمایشگاهی اثرات آنتی‌بیوتیک سالینومایسین در دوزهای مختلف (صفر، 2، 4، 8 و 16 میلی‌گرم بر لیتر) بر کینتیک تولید گاز، برخی شاخص‌های هضم و تخمیر شکمبه و در دوزهای صفر، 4 و 16 میلی‌گرم بر لیتر بر برخی جمعیت‌های میکروبی شکمبه با استفاده از روش Real-time PCR انجام شد. برای این منظور از سه رأس گوسفند نر فیستولادار جهت تهیه مایع شکمبه برای انکوباسیونهای 144 ساعته در مرحله اول و انکوباسیونهای 24 ساعته در مرحله دوم آزمایش استفاده شد. نتایج نشان داد که با افزایش دوز سالینومایسین گاز تولیدی در تمامی ساعات و حداکثر گاز تولیدی (A) بصورت خطی و درجه دو افزایش یافت. با این حال، نرخ تولید گاز ( µ) تحت تأثیر قرار نگرفت. همچنین میزان گاز تولیدی بعد از 24 ساعت (GP24)، میزان هضم آزمایشگاهی ماده خشک (IVTDMD) و ماده آلی (IVTOMD) و غلظت کل اسیدهای چرب فرار (TVFA) بموازات افزایش دوز سالینومایسین بطور خطی و درجه دو افزایش یافتند، اما فاکتور تفکیک (PF) کاهش یافت و توده میکروبی (MB) و غلظت آمونیاک تحت تأثیر سالینومایسین قرار نگرفتند. در بین میکروارگانیسم‌های شکمبه، جمعیت نسبی پروتوزوآها و فیبروباکترسوکسینوژنز در بالاترین دوز سالینومایسین کاهش یافت و جمعیت قارچها، متانوژنها و باکتری کلستریدیوم آمینوفیلوم تحت تأثیر سالینومایسین قرار نگرفت. این نتایج نشان داد که سالینومایسین اثرات مثبتی بر شاخص‌های هضم و تخمیر شکمبه‌ای دارد که با توجه به یافته های نوین مبنی بر اثرات آن در پیشگیری از انواع سرطان در انسان می‌تواند در تغذیه دام به‌عنوان افزودنی استفاده گردد. | ||
| کلیدواژه‌ها | ||
| یونوفر؛ سالینومایسین؛ تخمیر شکمبه؛ میکروبهای شکمبه؛ Real-time PCR | ||
| عنوان مقاله [English] | ||
| In vitro evaluation of dose-response effects of salinomycin antibiotic on rumen fermentation and some rumen microorganisms using Real-time PCR | ||
| نویسندگان [English] | ||
| Nasibeh Navidi Moghadam1؛ Mostafa Malecky2؛ Asghar Mirzaei Asl3 | ||
| 1Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University | ||
| 2Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran | ||
| 3Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University | ||
| چکیده [English] | ||
| The present study was conducted to assess the effects of salinomycin antibiotic at different doses (0, 2, 4, 8 and 16 mg/L) on gas production kinetics, some rumen digestion and fermentation indices, and at the doses of 0, 4 and 16 mg/L on some rumen microbial populations using Real-time PCR method. For this purpose, three ruminally fistulated male rams were used to collect the rumen fluid for incubations of 144 h in the first and incubations of 24 h in the second phase of the experiment. Results indicated that the gas produced at all the measured times and the asymptotic gas production (A) increased linearly and quadratically with increasing doses of salinomycin. However, the gas production rate (µ) was not affected by the treatment. The gas produced after 24 h of incubation (GP24), in vitro true dry matter degradability (IVTDMD), in vitro true organic matter degradability (IVTOMD) and total volatile fatty acid (TVFA) concentration increased in a linear and quadratic manner, but partitioning factor (PF) decreased and microbial biomass (MB) and ammonia concentration were not affected by salinomycin. Among the rumen microorganisms, the relative population of protozoa and Fibrobacter succinogens decreased at the highest dose of salinomycin and those of fungi, methanogens and Clostridium aminophilum remained unaffected by salinomycin. These results revealed that salinomycin has a positive impact on rumen digestion and fermentation indices, which regarding recent findings on its effects in preventing different kinds of human cancer, it can be used as feed additive in animal nutrition. | ||
| کلیدواژه‌ها [English] | ||
| Ionophore, salinomycin, rumen fermentation, rumen microbes, Real-time PCR | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 490 تعداد دریافت فایل اصل مقاله: 363 |
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