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بررسی اثر نوع محیط‌های کشت و تنظیم‌کننده‌های رشد بر صفات رشدی، ترکیب‌های بیوشیمیایی و مواد مؤثره ترخون (Artemisia dracuunculus L.) در شرایط درون شیشه ای | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 8، دوره 35، شماره 3 - شماره پیاپی 95، مرداد و شهریور 1398، صفحه 437-455 اصل مقاله (370.93 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2019.122953.2377 | ||
| نویسندگان | ||
| یاسمن شکوری1؛ بهاره کاشفی* 2 | ||
| 1فارغ‌التحصیل کارشناسی ارشد، گروه کشاورزی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران | ||
| 2استادیار، گروه کشاورزی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.35.437.95.3.1576.32 با توجه به اهمیت گیاه ترخون (Artemisia dracuunculus L.) از نظر دارویی و مشکلات موجود برای تکثیر آن می‌توان از تکنیک کشت بافت برای ازدیاد آن استفاده نمود. هدف از اجرای این مطالعه مقایسه محیط کشت‌های متفاوت و محرکهای شیمیایی بر صفات رشدی و ترکیب‌های بیوشیمیایی ترخون بود. تیمارهای آزمایش شامل محیط کشت‌های موراشیگ و اسکوگ (MS)، گامبورگ (B5) و شنک و هیلدبرانک (SH) و همچنین تنظیم‌کننده‌های رشد کینتین، NAA، 2,4-D به تنهایی و ترکیب NAA + کینتین و ترکیب 2,4-D + کینتین با غلظت 0.5 میلی‌گرم در لیتر بودند. این تحقیق به صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار انجام گردید. نتایج حاصل از این تحقیق نشان داد که تیمارهای محیط کشت و تنظیم‌کننده‌های رشد اثر معنی‌داری بر خصوصیات کالوس، گیاهچه باززایی شده، ترکیب‌های بیوشیمیایی کالوس، اسانس گیاه و ترکیب‌های آن داشت. محیط کشت MS سبب شد درصد بالاتر کالزایی و وزن تر و خشک آن به میزان بیشتری افزایش یابد. بیشترین درصد باززایی (35.5%)، طول ریشه (1.28 سانتی‌متر)، وزن خشک ریشه (6.5 گرم) و وزن خشک ساقه (2.6 گرم) در محیط کشت MS، NAA+ کینتین و 2,4-D+ کینتین حاصل شد. همچنین میزان فلاونوئید، میزان فنول و ترکیب‌های فنولی در کالوس ترخون در محیط کشت MS، NAA+ کینتین و 2,4-D+ کینتین بالاتر بود. میزان اسانس (2.42%) و اوسیمون (5.78%) در محیط کشت MS، NAA+ کینتین و 2,4-D+ کینتین بیشتر از سایر تیمارها بود. همچنین درصد استراگول با کاربرد 2,4-D افزایش یافت و NAA سبب افزایش درصد اوسیمون، لیمونن و لینانول موجود در اسانس گردید. درصد لیمونن با کاربرد تنهای NAA و درصد لینانول در تیمار 2,4-D+ کینتین بیشتر از سایر تیمارها بود. نتایج نشان داد که بالاترین شاخص رشدی، ترکیب بیوشیمیایی و اسانس در محیط کشت پایه MS حاوی (تنظیم‌کننده‌های رشد) مشاهده شد. همچنین کاربرد کینتین به همراه NAA و 2,4-D سبب بهبود رشد کالوس، باززایی، اسانس و برخی ترکیب‌های موجود در اسانس ترخون گردید. | ||
| کلیدواژه‌ها | ||
| اسانس؛ اکسین؛ کالوس؛ کشت بافت؛ گیاه دارویی؛ سیتوکینین | ||
| عنوان مقاله [English] | ||
| Effects of culture medium and growth regulators on growth traits and secondary metabolites of Artemisia dracunculus L. under in vitro conditions | ||
| نویسندگان [English] | ||
| Y. Shakoori1؛ B. Kashefi2 | ||
| 1Graduated student, College of Agriculture, Islamic Azad University, Damghan Branch, Damghan, Iran | ||
| 2College of Agriculture, Islamic Azad University, Damghan Branch, Damghan, Iran | ||
| چکیده [English] | ||
| Considering the importance of medicinal properties of tarragon, and its propagation problems, tissue culture techniques can be used for its propagation. The aim of this study was to compare different culture media and chemical plant growth regulators on the growth traits and secondary metabolites of tarragon (Artemisia dracunculus L.). Treatments were culture medium at three levels of Murashige and Skoog (MS), Gamborg (B5) and Schenk & Hildebrandt (SH), and growth regulators at five levels of Kinetin (KIN), NAA, and 2,4-D alone (each with a concentration of 0.5 mg l-1), and two combinations of NAA+KIN and 2,4-D+KIN. The present study was conducted as a factorial based on completely randomized design with four replications. The results showed that culture media and growth regulators treatments had significant effects on callus characteristics, regenerated plantlets, callus secondary metabolites, and plant essential oil and its composition. MS medium caused a higher callus induction percentage and more increase in its fresh and dry weight. MS with NAA+KIN and 2,4-D+KIN caused the highest regeneration percentage (35.5), root length (1.28 cm), root (6.5 g) and stem (2.6 g) dry weight, the amount of callus phenolics, and also the amount of essential oil (2.42%) and ocimene (5.78%). Estragol percentage of essential oil increased by 2,4-D, while NAA enhanced ocimene, limonene, and linalool. The percentage of limonene in treatment with NAA alone and linalool in treatment with 2,4-D+ KIN was higher than those of other treatments. The results showed that the highest growth index, callus secondary metabolites, and plant essential oil were observed in MS medium containing growth regulators. Also, the application of kinetin with NAA and 2, 4-D resulted in improved callus growth, regeneration, essential oil, and some of its compounds production in tarragon. | ||
| کلیدواژه‌ها [English] | ||
| Essential oil, auxine, callus, tissue culture, medicinal plant, cytokinin | ||
| مراجع | ||
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- Abdelnaser, A., Elzaawely, T., Xuan, D. and Shinkichi T., 2007. Changes in essential oil, kava pyrones and total phenolics of Alpinia zerumbet (Pers.) B. L. Burtt. and R. M. Sm. leaves exposed to copper sulphate. Environmental and Experimental Botany, 59: 347-353. - Alizadeh, M., 2012. Tissue culture and micropropagation manual. Norouzi Press, Gorgan, Iran, 322p. - Amany, K., Ibrahim, S. and Ahmed, A., 2011. Efficient callus induction, plant regeneration and estragole estimation in tarragon (Artemisia dracunculus L.). Journal of Essential Oil Research, 23: 16-20. - Asareh, M.H., Gharbanli, M., Allahverdi Mamaghani, B., Zare, A. and Shahrzad, Sh., 2007. Effect of culture medium and plant growth regulators on in-vitro propagation of Rose. Research and Convinience, 72: 45-51. - Asghari, G.R., Ghasemi, R., Yousefi, M. and Mahdinejad, N., 2015. Effect of PGRs and kitozan on phenolic components in callus and seedling of dermane. Plant Process and Function Journal, 3(10): 93-100. - Atrashi, M. and Karimi Dehkordi, R., 2015. Study on different concentration of PGRs on micropropagation of tamato at in-vitro condation. Green Scince Prin Journal, 5(19): 127-133. - Bell, R.L., Srinivasan, C. and Lomberk, D., 2009.Effect of nutrient media on axillary shoot proliferation and preconditioning for adventitious shoot regeneration of pears. In vitroCell Devission Biology, 45: 708-714. - Chang, Y.L., Kim, D.O., Lee, K.W., Lee, H.J. and Lee, C.Y., 2002. Vitamin C equivalentanti oxidant capacity (VCEAC) of phenolic phytochemicals. Journal of Agriculture and Food, 50(13): 3713-3717. - Chappell, J., 1995. Biochemistry and molecular biology of the isoprenoid biosynthetic pathway. Annals in Review in Plant Physiology and Molocular Biology, 46: 521-547. - Morzadec, J.M. and Hourmant, A., 1997. In vitro rooting improvement of globe artichoke (cv. Camus de Bretagne) by GA3. Scientia Horticulturae, 72: 59-62. - Diettrich, B., Mertinat, H. and Luckner, M., 1990. Formation of Digitalis lanata clone lines by shoot tip culture. Planta Medicine, 56: 53-58. - Duke, J.A., 2001. Handbook of Medicinal Herbs. CRC Press, LLC.USA, 896p. - El-Khateeb, M.A., 1994. Effect of some growth regulators on growth, fruit yield and essential oil in dill plant. Bulltein of Faculty of Agriculture University of Cairolina, 45: 187-205. - Flexas, J. and Medrano, H., 2002. Drought-inhibition of photosynthesis in C3 plants: stomatal and nonstomatal limitations revisited. Annals of Botany, 89: 183-89. - Trejo-Tapia, G., Amaya, U.M., Guadalupe, S.M., Sánchez, A.D.J., Martínez, B., Rodríguez-Monroy, M. and Jiménez-Aparicio, A.R., 2002. The effect of cold-pretreatment, auxins and carbon source on anther culture of rice. Plant Cell, Tissue and Organ Culture, 71: 41-46. - Ghasemzadeh, A., Jaafar, H.Z. and Rahmat, A., 2010. Antioxidant activities, total henolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecule, 15(6): 4324-4333. - Hadian, J., Asgari-Lajayer, H., Moteshare-zadeh, B. and Ghorbanpour, M., 2016. Evaluation of essential oils content and yield of Satureja hortensis in response to different copper and zinc treatments. Iranian Journal of Plant Biology, 7(24): 53-66. - Hosseini, B. and Bighamat, A., 2017. Effects of different concentrations of growth regulators and explants type on callus induction, embryogenesis and shoot regeneration of Origanum vulgare ssp. Gracile. Iranian Journal of Renglands and Forests Plant Breeding and Genetic Research, 24(2): 264-276. - Jeong, G.T., Woo, J.C. and Park, D.H., 2007. Effect of plant growth regulators on growth and biosynthesis of phenolic compounds in genetically transformed hairy roots of Panax ginseng C. A. Meyer. Biotechnology and Biopathology Enginereeng, 12: 86-91. - Karimi, M., Kazemitabar, S.K., Azadbakht, M. and Nematzadeh, G., 2015. Study of tissue culture of digitaris. Crop Breeding Journal, 6(13): 18-28. - Kaviani, B., Ahmadi, A., Torang, A.R., Bohluli, S. and Hashemabadi, B., 2014. Effect of NAA and kinetin on micropropagation of Sabbu. Plant Science Research Journal, 8(2): 32-39. - Keikhaakhar, F., Khadem, A. and Sharifi, A., 2013. Improving of tissue culture of Lavandula angustifolia Mill. 3rd Iranian Agricultural Biotechnology Conference, Mashhad, Iran, 3 September, 5p. - Kiarostami, K. and Mosafa, N., 2016. Srudy of antioxidant benefits of angustifolia under in-vitro condition. Plant Science Research Journal, 8(4): 835-843. - Koohi, L., Zare, N., Asgharizakaria, R. and Sheykhzadeh, P., 2015. Role of plant growth regulators on tissue culture response and cell suspension of Babune. Crop Ecophysiology Journal, 2(30): 203-214. - Kumari, N. and Saradhi, P., 1992. Regeneration of plants from callus cultures of Origanum vulgare. Plant Cell Reproductive, 11(9): 476-479. - Lamina, A., Naghdibadi, H., Ladan-Moghadam, A. and Mehrafarin, A., 2016. Changes of morphophysiological traits, essentioal oil and methylchavichol of tarragon in response to micorhyza inoculation and salt stress. Medicinal Plant Journal, 14(4): 64-77. - Mehrabani, B., Nazeri, S. and Piri, K., 2013. Assesing of total phenol in muintane tea via tissue culture and possibility of it increasing with stimulators. Agriculture Biotechnology Journal, 4(2): 77-88. - Misra, A. and Sharma, S., 1991. Critical Zn concentration for essential oil yield and menthol concentration of Japanese mint. Fertilizer Research, 29: 261-265. - Muanda, F.N., Soulimani, R., Diop, B. and Dicko, A., 2011. Study on chemical composition and biological activities of essential oil and extracts from Stevia rebaudiana Bertoni leaves. LWT-Food Science and Technology, 44(9): 1865-1872. - Munsur, M., Haque, M. and Nasiruddin, K., 2009. In vitro propagation of bitter gourd (Momordica charantia L.) from nodal and root segments and M.S. Hossain. Plant Tissue Culture Biotechnology, 19(1): 45-52. - Nabila, S., Fawzia, M., Naser, A. and Rida, A., 2003. Growth and rosmarinic acid accumulation in callus, cell suspension, and root cultures of wild Salvia fruticosa. Plant Cell Tissue and Organ Culture, 73: 117-121. - Nikolaeva, T.N., Zagoskina, N.V. and Zaprometov, M.N., 2009. Production of phenolic compounds in Callus Cultures of Tea plants under the effect of 2,4-D and NAA. Russian Journal of Plants Physiology, 56(1): 45-49. - Nourafcan, H. and Ansari, F., 2016. The effect of MS and B5 media on growth indices of lemon verbena in in vitro condition. Iranian Journal of Horticultural Science, 48(1): 249-252. - Olszewska-Kaczynska, I. and Suchorska, K., 1996. Characterization of tarragon (Artemisia dracunculus L.) cultivated in Poland. Herba Polanica, 42: 5-10. - Omidbeigi, R., 2010. Production and Prossesing of Medicinal Plants Facts (Vol. 2). Astan Ghods Razavi, 424p. - Palazon, J., Bonfill, M. Cusido, R.M., Pinol, M.T. and Morales C., 1995. Effect of auxin and phenobarbital on morphogenesis and production of digitoxin in Digitalis callus. Plant Cell Physiology, 36(2): 247-252. - Pazoki, A., Fahimi, H. and Shakeri, H., 2008. Effact of NAA and IAA on quality and quantity of essential oil of tarragon. Research Convenience Journal, 74: 124-128. - Renata, N.W. and Grażyna, Z., 2014. Herb yield and bioactive componds of tarragon (Artemisia dracunculus L.) as influenced by plant density. Acta scientiarum Polonorum. Hortorum cultus, 13(2): 207-221. - Roozban, M.R., Arzani, K. and Moieni, A., 2002. Study on in vitropropagation of some Asian pear. Pakistan Journal of Biological Science, 10(22): 4118-4122. - Sangwan, N., Farooqi, A.H.A., Shabih, F. and Sangwan R.S., 2001. Regulation of essential oil production in plants. Journal of Plant Growth Regulator, 34: 3-21. - Sayyah, M., Nadjafinia, L. and Kamalinejad, M., 2004. Anticonvulsant activity and chemical composition of Artemisia dracunculus L. essential oil. Journal of Ethnopharmacology, 94(2): 283-287. - Sifola, M. and Barbieri, G., 2006. Growth, yield and essential oil content of three cultivars of basil grown under different levels of nitrogen in the field. Sciencia Horticulture, 108: 408-413. - Taji, A.M., Dodd, W.A. and Williams, R.R., 1997. Plant Tissue Culture Practice. University of New England press, Armidale, Australia, 258p. - Tanoori, A., Ghaseemnejad, A. and Alizadeh, M., 2015. Effect of Metiljasmonat and salisilic acid on morphological traits and choromoplast of Kangar callus. Agricultural Behzeraee Journal, 16(4): 857-869. - Tatari, M., Asgari, N. and Nosrati, S.Z., 2010. Improving of in-vitro culture of Gerbera cv. Tropical Blend Agriculture Behzeraee Journal, 25(4): 389-401. - Tripathi, L. and Tripathi, J.N., 2003. Role of biotechnology in medicinal plants. Tropical Journal of Pharmacy Research, 2(2): 243-253. - Vandersalm, T.P.M., Vandertorn, C.J.G. and Hanischtencate, C.H., 1994; Importance of iron chelate formula for micropropagation of Rosa hybrida L. money way. Plant Cell Tissue and Organ Culture, 37: 73-77. - VanStaden, D., 2008. Plant growth regulators, II: cytokinins, their analogues and inhibitors: 205-226. In: George, E.F., Hall, M.A. and De Klerk, G.J., (Eds.). Plant Propagation by Tissue Culture. Volume 1, The Background. Springer, 477p. - Verma, P. and Sen, N.L., 2009. The impact of plant growth regulators on growth and biochemical constituents of coriander (Coriandrum sativum L.). Journal of Herbs Spices & Medicinal Plants, 14: 144-153. - Wani, M., Pande, S. and More, M., 2010. Callus induction studies in Tridax procumbents. International Journal of Biotechnology Application, 2(1): 11-14. - Wu, Y., Taylor, K., Biswas, N. and Bewtra, J., 1998. A model for the protective effect of additives on the activity of horseradish peroxidase in the removal of phenol. Enzyme and Microbial Technology, 22(5): 315-322. - Yasmin, S., Nasiruddin, K.M., Begum, R. and Talukder, SK., 2003. Regeneration and establishment of potato plantlets through callus formation with BAP and NAA. Asian Journal of Plant Science, 2: 936-940. - Yen, Y.K., Lee, S.Y., Woo, T.P., Park, N. and Park, S., 2010. Exogenous auxins and polyamines enhance growth and rosmarinic acid production in root cultures of Nepeta cataria L. Plant Omics Journal, 3(6):190-193. - Zaidi, M.A., Narayanan, R., Sardana, I., Taga, S., Postel, R., Johns, M., McNulty, Y., Mottiar J., Mao, E., Loit, I. and Altosaar I., 2006. Optimizing tissue culture media for efficient transformation of different indica rice genotypes. Agronomy Research, 4(2): 563-575. | ||
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