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فرمانتاسیون جامد و ماندگاری Pochonia chlamydosporia var. chlamydosporia و Purpureocillium lilacinum روی بسترهای آلی | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 1، دوره 6، شماره 2 - شماره پیاپی 12، اسفند 1397، صفحه 1-14 اصل مقاله (232.88 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2019.119398 | ||
| نویسندگان | ||
| راحله سادات شیرازی1؛ صدیقه فاطمی* 2؛ شهرام نعیمی2 | ||
| 1گروه گیاه‌پزشکی، دانشکده علوم کشاورزی و صنایع‌غذایی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| قارچ‌هایvar. chlamydosporia Pochonia chlamydosporia و Purpureocillium lilacinumاز عوامل کنترل بیولوژیک مهم نماتدهای گره ریشه و سیست نماتدها هستند. هر دو قارچ اولین بار از نماتد سیست چغندر قند از خراسان جدا سازی شدند و کارآیی امیدوار کننده‌ای روی کنترل نماتدهای Heterodera schachtii،Globodera rostochiensis و Meloidogyne spp. نشان داده‌اند. در این مطالعه میزان اسپورزایی و ماندگاری قارچ ها روی بسترهای جامد طی دو ماه بررسی شد. قارچ‌ها روی بسترهای غلات شامل بذر گندم، بذر جو، سبوس گندم، سبوس برنج و پوسته شلتوک کشت داده شدند و اسپور دهی و پایداری آن‌ها در شرایط دمای اتاق و یخچال ارزیابی شد. بسته به نوع بستر، زمان نگهداری و دما و نیز میزان اسپوردهی قارچ‌ها متفاوت بود. P. chlamydosporia روی بذرگندم، بذر جو، سبوس گندم و سبوس برنج پس از 30 روز و روی پوسته شلتوک پس از دو ماه اسپوردهی نمود. P. lilacinumهمه بسترها را پس از 30 روز کلونیزه کرد و روی بذر جو و پوسته شلتوک برنج بیشترین اسپورزایی را داشت. ماندگاری P. chlamydosporia و P. lilacinum پس از 60 روز نگهداری در 25 درجه سلسیوس مقداری کاهش نشان داد، ولی در دمای10 درجه سلسیوس تغییر معنی‌داری نیافت. بالاترین جمعیت پوکونیا روی سبوس‌های گندم و برنج به میزان 108 × 5/1 و روی بقیه بسترها 107 × 7 اسپور در هر گرم بستر و برای پورپوریوسیلیوم روی سبوس گندم و جو به ترتیب 108 × 7/2 و 108 × 5/1 اسپور در هر گرم پس از 60 روز بود. کلامیدوسپورها مرحله استراحتی پوکونیا و یکی از رایج ترین منابع تلقیح در خاک هستند. پوکونیا روی جو جمعیتی معادل 105 × 1/1 و روی سبوس برنج و گندم معادل 103 × 5 کلامیدوسپور تولید نمود. | ||
| کلیدواژه‌ها | ||
| زنده‌مانی؛ بسترهای کشت؛ تولید انبوه؛ غلات | ||
| عنوان مقاله [English] | ||
| Solid-state fermentation and viability of Pochonia chlamydosporia and Purpureocillium lilacinum on some organic substrates | ||
| نویسندگان [English] | ||
| Rahele Sadat Shirazi1؛ Seddigheh Fatemy2؛ Shahram Naeimi2 | ||
| 1Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science & Research Branch of Islamic Azad University, Tehran, Iran | ||
| 2Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extention Organization (AREEO), Tehran, Iran | ||
| چکیده [English] | ||
| Pochonia chlamydosporia and Purpureocillium lilacinum are important biocontrol agents of root-knot and cyst nematodes. Both fungi were first reported from sugar beet cyst nematode from Khorasan province and have shown promising potential as biocontrol agents of Heterodera schachtii, Globodera rostochiensis and Meloidogyne spp. In this study, sporulation and shelf life of fungi were evaluated on solid substrates for 2 months. The fungi were grown on cereals, their spore production and stability at 10 °C and 25 °C were evaluated. Based on media, storage duration and temperature regime, spore production of fungi differed. Pochonia produced spores on wheat grain, barley grain, wheat and rice bran after 30 days, and on rice husk after 60 days. P. lilacinum colonizedall the substrates and yield of spore was highest on barley grain and rice husk. Viability of both fungi was slightly decreased after 60 days storage at 25 ºC, but at 10 ºC with some discrepancy did not change significantly. After 60 days, Pochonia produced average 1.5 × 108 and 7 × 107 spores / g of wheat and rice bran, and other cereals, respectively. Meanwhile, P. lilacinum produced 2.7 × 108 and 1.5 × 108 spores /g wheat and barley bran, respectively. Chlamydospores are resting spores of P. chlamydosporia var. chlamydosporia and one of the most common sources of inoculation in soil. On barley 1.1× 105 and on wheat and rice bran average 5 × 103 chlamydospores were produced. | ||
| کلیدواژه‌ها [English] | ||
| cereals, culture substrates, mass production, storage | ||
| مراجع | ||
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Abd-Elgawad, M.M.M. 2014. Plant-parasitic nematodes threats to global food security. Journal of Nematology, 46: 130–260. Amala, U., Jiji, T. & Naseema, A. 2012. Mass multiplication of entomopathogenic fungus Paecilomyces lilacinus (Thom) Samson with solid sunstrates. Journal of Biopesticides, 5(2): 168–170. Atkins, S.D., Clark, I.M., Hirsch, P.R. & Kerry, B.R. 2005. The use of real-time and species specific primers for the identification and monitoring of Paecilomyces lilacinus. FEMS Microbiology Ecology, 51: 257–264. Ayatollahy, E., Fatemy, S. & Etebarian, H.R. 2008. Potential for biological control of Heterodera schachtii by Pochonia chlamydosporia var. chlamydosporia on sugar beet. Biocontrol Science & Technology, 18: 157–167. Bourne, J.M. & Kerry, B.R. 2000. Observations on the survival and competitive ability of the nematophagous fungus Verticillium chlamydosporium in soil. International Journal of Nematology, 10: 9–18. Cabanillas, E. & Barker, K.R. 1989. Impact of Paecilomyces lilacinus inoculum level and application time on control of Meloidogyne incognita on tomato. Journal of Nematology, 21, 1: 115–120. Clyde, J.M.F. 1993. The cyst nematode pathogen Verticillium chlamydosporium. PhD Thesis, The University of Leeds, Department of Pure and Applied Biology. Crump, D.A. & Irving, F. 1992. Selection of isolates and methods of culturing Verticillium chlamydosporium and its efficacy as a biological control agent of beet and potato cyst nematodes. Nematologica, 38: 367–374. De Leij, F.A.A.M. & Kerry, B.R. 1991. The nematophagous fungus, Verticillium chlamydosporium Goddard, as a biological control agent for Meloidogyne arenaria (Neal) Chitwood. Revue de Nematologie, 14: 157–194. Dehghan Nasrabd, A. & Fatemy, S. 2016. Effect of Pochonia chlamydosporia var. chlamydosporia on potato cyst nematode. 3rd National meeting on Biocontrol in Agriculture and Natural Resources, 2-3 Feb, Ferdowsi University, Mashad, Iran. 89. Dube, B. & Smart, G.C.Jr. 1987. Biological control of Meloidogyne incognita by Paecilomyces lilacinus and Pasteuria penetrans. Journal of Nematology, 19, 2: 222–227. Ebadi, M., Fatemy, S. & Riahi, H. 2009. Evaluation of the efficacy of Pochonia chlamydosporia var. chlamydosporia as a biological control agent of Meloidogyne javanica on pistachio. Biocontrol Science and Technology, 19(7): 689–700. Ebadi, M., Fatemy, S. & Riahi, H. 2018. Biocontrol potential of Pochonia chlamydosporia var. chlamydosporia isolates against Meloidogyne javanica on pistachio. Egyptian J. Biological Pest Control 28(45): doi.org/10.1186/s41938-018-0047-y. Fatemy, S., Ahmadian Yazdi, A., Ahmadi, A., Parvizy, R., Pakniat, M., Barooti, S., Askari, F. & Ershad, J. 1999. Fungal parasites of Heterodera schachtii in Iran. Pakistan J. Nematology, 7, 1: 61–66. Flores-Camacho, R., Manzanilla-Lopez, R.H., Ciddel Prado-Vera, I., & Martnez-Garza, A. 2007. Control of Nacobbus aberrans (Thorne) Thorne and Allen with Pochonia chlamydosporia (Goddard) Gams and Zare. Revista Mexicana Fitopatologia, 25: 26–34. Gao, L. 2011. Optimize culture conditions for biomass and sporulation of the nematophagous fungus Pochonia chlamydosporia, isolate HSY-12-14 by “two-step” cultivation. African Journal of MicrobiologyResearch, 5: 4731–4738. Gintis, B.O., Morgan-Jones, G. & Rodriguez-kabana, R. 1983. Fungi associated with several developmental stages of Heterodera glycines from an Alabama soybean field soil. Nematropica, 13: 181–200. Griffin, D.H. 1994. Chemical requirements for growth. pp. 130–157. In: Griffin, D.H. (ed). Fungal Physiology, 2nd, Wiley-Liss. New York. Hadapad, A.B. & Zebita, C.P. 2006. Mass production, survival and evaluation of solid substrate inocula of Beauveria brongniartii (Saccardo) Petch against Holotrichia serrata (Coleoptera: Scarabaeidae). Communications in Agricultural and Applied Biological Sciences, 71, 2B: 433–441. Hallman, J., Davies, K.G. & Sikora, R. 2009. Biological control using microbial pathogens, endophytes and antagonists. In: Perry, R.N., Moens, M. & Starr, J.L. (eds.), Root-knot Nematodes. CABI, UK. Hazmi, A.S., Yahya, F.A., AbdelRafaa, O.A. & Lafi, H.A. 2019. Effects of humic acid, Trichoderma harzianum and Paecilomyces lilacinus on Meloidogyne javanica. International Journal of Agriculture, Environment and Bioresearch, 4(1): 61–74. Heijbroek, W. 1983. Some effects of fungal parasites on the population development of the beet cyst nematode (Heterodera schachtii Schm.). Communications in Agricultural and Applied Biological Sciences, 48: 433–439. Hernández, M.A. & Hidalgo-Díaz, L. 2008. KlamiC: Bionematicida agrícola producido a partir del hongo Pochonia chlamydosporia var. catenulata. Revista de Protección Vegetal, 23: 131–134. Hidalgo-Diaz, L., Bourne, J.M., Kerry, B.R. & Rodriguez, M.G. 2000. Nematophagous Verticillium spp. in soils infested with Meloidogyne spp. in Cuba: Isolation and screening. International Journal of Pest Management, 46(4): 277–284. Hidalgo-Diaz, L., Franco-Navarro, F. & de Freitas, L.G. 2017. Pochonia chlamydosporia microbial products to manage plant –parasitic nematodes: Case studies from Cuba, Mexico and Brazil. pp. 311-342. In: Manzanilla-López, R.H., Lopez-Llorca, L. V. (eds.), Prospectives in sustainable nematode management through Pochonia chlamydosporia applications for root and rhizosphere health. Springer. Irving, F. & Kerry, B.R. 1986. Variation between strains of the nematophagous fungus Verticillium chlamydosporium Goddard. Factors affecting parasitism of cyst nematode eggs. Nematologica, 32: 474–485. Jamalirad, N., & Fatemy, S. 2013. Pathogenicity of Pochonia chlamydosporia var. chlamydosporia on root-knot nematode on cucumber. 1st National Conference on Sustainable Agriculture Development and Healthy Environment, 26 Feb. Islamic Azad University, Hamandan, Iran. 1–12, (in Persian) Jaronski, S.T. 2014. Mass production of entomopathogenic fungi: state of art. pp. 357-413. In: Morales-Ramos, J.A.; Guadalupe Rojas, M.; Shapiro-llan, D. (eds.), Mass production of beneficial organisms, invertebrates and entomopathogens. Elsevier Inc. https://doi.org/10.1016/B978-0-12-391453-8.00011-X Jones, K.A. & Burges, H.D. 1998. Technology of formulation and application. Pp 7-30. In: Burges, H.D (eds.), Formulation of microbial biopesticides. Kluwer Academic Publishers, Dordrecht. Jones, J.T., Haegeman, A., Danchin, E.G.J., Gaur, H.S., Helder, J., Jones, M.G.K., Kikuchi, T., Manzanilla-Lopez, R., Palomares-Rius, J.E., Wesemael, W.M.L. & Perry, R.N. 2013. Top 10 plant parasitic nematodes in molecular plant pathology. Molecular Plant Pathology, 14: 946–961. Karakas, M., Benli, M. & Cebesoy, S. 2012. Effects of some cultural conditions on the growth of nematophagous fungus Pochonia chlamydosporia (Fungi: Clavicipitaceae) isolated from Meloidogyne incognita eggs. Journal of Animal and Veterinary Advances, 11(24): 4644–4647. Kerry, B.R. 1975. Fungi and the decrease of cereal cyst-nematode populations in cereal monoculture. EPPO Bull, 5:353–361. Kerry, B.R. 2000. Rhizosphere interactions and the exploitation of microbial agents for the biological control of plant-parasitic nematodes. Annual Review of Phytopathology, 38: 423–441. Kerry, B.R. & Hidalgo-Diaz, L. 2004. Application of Pochonia chlamydosporia in the integrated control of root-knot nematodes on organically grown vegetable crops in Cuba. Multitrophic Interactions in the Integrated Control, 27: 123–126. Kerry, B.R., Crump, D.H. & Mullen, L.A. 1982. Studies of the cereal cyst-nematode, Heterodera avenae, under continuous cereals, 1974–1978. II. Fungal parasitism of nematode females and eggs. Annals of Applied Biology, 100: 489–499. Kerry, B.R., Kirkwood, I.A., De Leij, F.A.A.M., Barba, J., Leijdens, M.B. & Broes, P.C. 1993. Growth and survival of Verticillium Goddard, a parasite of nematodes in soil. Biocontrol Science and Technology, 3: 355–365. Larriba, E., Jaime, M., Carbonell-Caballero, J., Conesa, A., Dopazo, J., Nislow, C., Martin-Nieto, J. & Lopez-Llorca, L. 2014. Sequencing and functional analysis of the genome of a nematode egg-parasitic fungus Pochonia chlamydosporia. Fungal Genetics and Biology, 65: 69–80. Lamovsek, J., Urek, G. & Trdan, S. 2013. Biological control of root knot nematodes (Meloidogyne spp.): Microbes against pests. Acta Agriculture Slovenia, 101(2): 263–275. Loffredo, A., Bent, E., McKenry, M.V., Borneman, J. & Becker, Jo. 2007. Understanding a root-knot nematode suppressive soil. Journal of Nematology, 39: 86. Luambano, N.D., Manzanilla-Lopez, R.H., Kimenju, J.W., Powers, S.J., Naria, R.D., Wanjohi, W.J. & Kerry, B.R. 2015. Effects of temperature, pH, carbon and nitrogen ratios on the parasitic activity of Pochonia chlamydosporia on Meloidogyne incognita. Biological Control, 80: 23–29. Magan, N. 2001. Physiological approaches to improving the ecological fitness of fungal biocontrol agents. Pp 239-251. In: Jackson TMC, Magan, N. (eds.), Fungi as biocontrol agents: progress, problems and potential. CABI, UK. Maheshwari, R. 2012. Fungi. Experimental methods in biology, 2nd ed. Boca Raton: CRC Press. Manzanilla-López, R.H., Esteves, I., Powers, S.J. & Kerry, B.R. 2011. Effects of crop plants on abundance of Pochonia chlamydosporia and other fungal parasites of root-knot and potato cyst nematodes. Annals of Applied Biology, 159: 118–129. Mar, T.T. & Lumyong, S. 2012. Conidial production of entomopathogenic fungi in solid-state fermentation. KKU Research Journal, 17(5): 762–768. Mascarin, G.M., Alves, S.B. & Biaggioni Lopes, R. 2010. Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa. Brazilian Archives of Biology and Technology, 53(4): 753–761. Mauchline, T.H., Kerry, B.R. & Hirsch, P. 2002. Quantification in soil and the rhizosphere of the nematophagous fungus Verticillium chlamydosporium by competitive PCR and comparison with selective plating. Applied and Environmental Microbiology, 68: 1846–1853. McKevith, B. 2004. Nutritional aspects of cereals. Nutrition Bulletin, 29: 111–142. Meyer, S.L.F., Huettel, R.N., Liu, X.Z., Humber, R.A. & Juba, J. 2004. Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile motility. Nematology, 6: 23–32. Moosavi, M.R. & Askary, T.H. 2015. Nematophagous fungi commercialization. pp. 187–202. In: Askary, T.H. & Martinelli, P.R.P. (eds.). Biocontrol Agents of Phytonematodes. CABI, UK. Moosavi, M., Zare, R., Zamani zadeh, H. & Fatemy, S. 2010. Pathogenicity of Pochonia species on eggs of Meloidogyne javanica. Journal of Invertebrate Pathology, 104: 125–133. Rao, M.S., Kerry, B.R., Gowen, S.R., Bourne, J.M., & Reddy, P.P. 1997. Management of Meloidogyne incognita in tomato nurseries by integration of Glomus deserticola with Verticillium chlamydosporium. Z Pflanzenk Pflanzen – J. Plant Diseases and Protection, 104: 419–422. Ravensber, W.J. 2011. A roadmap to the successful development and commercialization of microbial pest control products for control of arthropods. Springer. Reddy, P.P., Khan, R.M. & Rao, M.S. 1991. Integrated management of the citrus nematode Tylenchulus semipenetrans using oil-cakes and Paecilomyces lilacinus. Afro-Asian Journal of Nematology, 1, 2: 221–222 Rumbos, C.I. & Kiewnick, S. 2006. Effect of plant species on persistence of Paecilomyces lilacinus strain 251 in soil and on root colonization by the fungus. Plant and Soil, 283: 25–31. Sahayaraj, K. & Namasivayam, S.K.R. 2008. Mass production of entomopathogenic fungi using agricultural products and by products. African Journal of Biotechnology, 7(12): 1907–1910. Segers, R., Butt, T.M., Kerry, B.R., Beckett, A. & Peberdy, J.F. 1996. The role of the proteinase VCP1 produced by the nematophagous Verticillium chlamydosporium in the infection process of nematode eggs. Mycological Research, 100: 421–428. Stirling, G.R. 2011. Biological control of plant-parasitic nematodes: An ecological perspective, a review of progress and opportunities for further research. In: pp. 1-38. Davies, K.G. & Spiegel, Y. (eds.), Biological control of plant –parasitic nematodes: Building coherence between microbial ecology and molecular mechanisms, Progress in Biological Control. Springer. Stirling, G.R. 2014. Biological control as a component of integrated nematode management: the way forward. pp. 393–407.In: Stirling, G.R. (ed.) Biological control of plant parasitic nematodes: soil ecosystem management in sustainable agriculture, second revised edition. CABI, UK. Stirling, G.R. & Kerry, B.R. 1983. Antagonists of the cereal cyst-nematode Heterodera avenae Woll. in Australian soils. Australian Journal of Eexperimental Agriculture and Animal Husbandry, 23: 318–324. Sun, M.H., Gao, L., Shi, Y.X., Li, Bao. Jü. & Liu, X.Z. 2006. Fungi and Actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential. J. Invertebrate Pathology, 93: 22–28. Vieira dos Santos, M.C., Esteves, I., Kerry, B. & Abrantes, I. 2013. Biology, growth parameters and enzymatic activity of Pochonia chlamydosporia isolated from potato cyst and root-knot nematodes. Nematology, 15: 493– 504. Wagh, V.H. & Pramanik, A. 2014. Evaluation of Paecilomyces lilacinus for management of root knot nematode Meloidogyne incognita in tomato. The Bioscan, 9(1): 407–411. Wesemael, W.M.L., Viaene, N. & Moens, M. 2011. Root-knot nematodes (Meloidogyne spp.) in Europe. Nematology, 13: 3–16. Willcox, J. & Tribe, H.T. 1974. Fungal parasitism in cysts of Heterodera I. Preliminary investigations. Transactions of the British Mycological Society, 62: 585–594. Zaki, M.J. & Maqbool, M.A. 1993. Occurrence of Verticillium chlamydosporium egg parasite of root knot nematodes Meloidogyne Javanica and M. incognita in Pakistan. Journal of Nematology, 11: 37–40. Zare, R., Gams, W. & Evans, H.C. 2001. A revision of Verticillium section Prostrata. V. The genus Pochonia with notes on Rotiferophthora. Nova Hedwigia, 73: 51–86. | ||
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