| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,848 |
| تعداد مقالات | 32,322 |
| تعداد مشاهده مقاله | 41,444,073 |
| تعداد دریافت فایل اصل مقاله | 18,643,923 |
معرفی چند ژنوتیپ و رقم سیبزمینی مقاوم به ویروس برگ قاشقی‌سیب‌زمینی (PLRV) | ||
| آفات و بیماریهای گیاهی | ||
| مقاله 4، دوره 86، شماره 2 - شماره پیاپی 107، اسفند 1397، صفحه 163-169 اصل مقاله (134.68 K) | ||
| نوع مقاله: مدیریت آفات و بیماریهای گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2018.121165.1215 | ||
| نویسندگان | ||
| جعفر نیکان* 1؛ رضا پوررحیم2 | ||
| 1عضو هیئت علمی مرکز تحقیقات همدان | ||
| 2عضو هیات علمی بخش تحقیقات ویروس شناسی، موسسه تحقیقات گیاهپزشکی کشور | ||
| چکیده | ||
| بیماری برگ قاشقی سیبزمینی یکی از مهم‌ترین و شایعترین بیماریهای ویروسی سیبزمینی است و مانند سایر ویروس‌های بیماری‌زای گیاهی مؤثرترین روش مبارزه با آن استفاده از ارقام مقاوم است. در این تحقیق واکنش تعدادی رقم و ژرمپلاسم سیبزمینی در برابر آلودگی به ویروس برگ قاشقی سیبزمینی (Potato leafroll virus= PLRV) در یک آزمایش مزرعهای در قالب طرح بلوکهای کامل تصادفی با 12 تیمار و سه تکرار ارزیابی شد. مایه‌زنی بوته‌ها‌ی سیبزمینی با استفاده از شته سبز هلو حامل PLRV صورت گرفت. یک ماه پس از مایه زنی، بوتهها از نظر آلودگی به PLRV با استفاده از علایم ظاهر شده و آزمون الایزا بررسی شد. نتایج نشان داد که بین ژنوتیپهای سیبزمینی مورد بررسی از نظر میزان ابتلا به PLRV اختلاف معنی‌دار وجود دارد. ژرمپلاسم شماره 13/803970 بدون هیچ‌گونه آلودگی به‌عنوان ژنوتیپ خیلی مقاوم به PLRV ارزیابی گردید. رقم سانته مقاوم بود، رقم لیدیرزتا و ژنوتیپ شماره 31/397015 نسبتاً مقاوم و رقم دیامانت نسبتاً حساس بود. مابقی ارقام و ژرم‌پلاسم‌ها حساس یا بسیار حساس ارزیابی شدند. همچنین نتایج بیانگر همبستگی معنی‌دار 79 درصدی بین میزان آلودگی بر مبنای ظهور علایم و میزان آلودگی بر مبنای آزمون الایزا بود. | ||
| کلیدواژه‌ها | ||
| الایزا؛ ژرمپلاسم؛ واکنش؛ Potato leafroll virus | ||
| عنوان مقاله [English] | ||
| Introducing some Potato leafroll virus resistant potato genotypes and cultivars | ||
| نویسندگان [English] | ||
| Reza Pourrahim2؛ | ||
| 2Plant Disease Research Department, Iranian Research Institute of Plant Protection, P.O. Box: 1452, Tehran 19395, Iran Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| چکیده [English] | ||
| Potato leaf roll disease is one of the most important and widely distributed viral diseases of potato. Like other plant viruses, the use of resistant cultivars is the most effective control measure of this disease. In this study, the reactions of some potato cultivars and genotypes to Potato leafroll virus (PLRV) were evaluated in a field trial experiment. The experiment conducted as a randomized complete block design with 12 treatments and three replications. Each plot of the experimental design included a planting row of five potato plants of each cultivar/genotype. The experimental plants were then inoculated with the virus by putting 10 PLRV-carrying green peach aphids on each plant. One month after inoculation, the plants were examined for PLRV infection by observing symptoms development and using enzyme-linked immunosorbent assay (ELISA) test. The results revealed significant differences between the PLRV-infection rates of the potato cultivars/genotypes tested. The potato genotype 803970/13 with having no infected plant was evaluated as highly resistant to PLRV. The cultivar “Sante” was resistant, the cultivar “Lady Rosetta” and the genotype “397015/31” were moderately resistant, cultivar “Diamant” was moderately susceptible and the rest of genotypes or cultivars were found susceptible or highly susceptible. The results also showed a significant correlation (79%) between the infection rates of the test plants based on symptom development and those of the ELISA tests. | ||
| کلیدواژه‌ها [English] | ||
| ELISA, field trial, PLRV, reaction, resistant | ||
| مراجع | ||
|
BARKER, H. 1987. Multiple component of the resistance of potatoes to potato leafroll Virus. Annals of Applied Biology, No111: 641-648. BARKER, H. 1992. Potato leafroll. In Plant Diseases of Internation importance, Vol 11: Diseases of vegelables and oil lseed crop, pp. 124-144. Eds H. S., Chaube, U. S., Singh, A. N., MUKHOPADHYAY, and J., KUMAR. London: prentice Hall international. BARKER, H. 2001. Potato Leafroll. In Encyclopeddia of plant pathology. Vol 2: pp. 804. Eds: O. C., MALOY and T. D., MURRAY. New York. USA: John Wiley & sons inc. BARKER, H., WATERHOUSE, .P M. 1999. The development of resistance to luteovirus mediated by host genes and pathogen- derived transgenes. In The Luteoviridae. pp. 169-210. Eds: H. G., SMITH and H. BARKER. Wallingford. Oxon. Uk: CABI Publishing. BATOOL, A., ASLAM KHAN, N., FAROOQ, J., MUGHAL, S. M. and IFTIKHAR, S. 2011. ELISA-Based screening of potato germplasms against potato leafroll virus. Journal of Agricultural Research, No 49(1): 57-63. BEEKMAN, A. J. B. 1987. Breeding for resistance. In Viruses of potatoes and seed- potato production. pp. 162-170. Eds J. A. de Bokx and J. P. H VAN DER WANT. Wageningen: pudoc Wageningen. CLARK, M. F. and ADAMS, A. N. 1977. Characterization of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. Journal of General Virology, No 34: 475-83. D’ONGHIA, A.M., DJELOUAH, K., ALIOTO, D., CASTELLANO, M.A. and SAVINO, V. 1998. ELISA correlates with biological indexing for the detection of citrus psorosis-associated virus. Journal of Plant Pathology, 80 (2), 157-163. DANESH, D., FILSOOF, F. and DEHGHAN, M. 1993. Frequencies of four potato viruses in Faridan experimental potato fields, Isfahan province, Iran. Iranian Journal of Plant Protection, No 28: 1-9. ESKANDARI F, SYLVESTER E S, RICHARDSON J. 1979. Evidence of lack of propagation of potato leafroll virus in its aphid vector, Myzus persicae. Phytopathology, No 69: 45-47. HARRISON, B. D. 1984. Potato leafroll virus. Description of plant Viruses, No 291. Surrey, UK: The commonwealth Mycological Institute and Association of Applied Biologists. JAFARPOUR, B. 1995. Study on Potato leafroll virus in Mashhad. Proceedings of the 12th Iranian Plant Protection Congress, 2-7 September, Karaj, Iran, p162. JALALI, S., NEMATOLLAHI, M. R. and POURRAHIM, R. 2008. Investigation on spreading of viral infection of seed tuber potatoes and determination of infective indicator in Freidan region of Isfahan. Seed and Plant Improvement Journal, No 23(4) :505-514. KHAN, M. A. 2006. Identification of resistant sources against Potato leafroll virus and Myzus persicae Sulz. by biological tests and ELISA. Pakistan Journal of Phytopathology, No18(2): 191-198. KOJIMA, R. and LAPIERRE, H. 1998. Potato leafroll virus. In Europena Handbook of plant Diseases, pp. 23-24. Eds: I. M., SMITH, V., DUNEZ, D. H. PHILIPS, R. A., LELIOT, and S. A. ARCHER. Oxford: Blackwell Scientific publications. LOEBENSTEIN G. 2001. Potato leafroll virus. In Virus and Virus-like Diseases of Potatoes and Production of Seed-Potatoes, pp. 69-75. Eds G LOEBENSTEIN, P H BERGER, A A BRUNT AND R H LAWSON. Amsterdam: Kluwer Academic Publishers. PEETEN, H. M.G., SHIPPER, E., SHIPPER, J.K. and BAARVELD H.R. 2007. Netherlands catalogue of potato varieties. NIVAP, Den Haag, The Netherlands. POURRAHIM, R., FARZADFAR, S. and GOLNARAGHI, A. R. 2002. Plant viruses of Iran. Saman Co, Tehran. Iran. SALAZAR, L. F., QUERCI, M., BARTOLINI, I. and LAZARTE, V. 1995. Aphid transmission of potato spindle tuber viroid assisted by potato leafroll virus. Fitopatologia, No 30: 56-58. SOLOMON- BLACKBURN, R. M. and BARKER, H. 1993. Resistance to Potato leafroll luteovirus can be greatly improved by combining two Independent types of heritable resistance. Annals of Applied Bioligy, No122: 329-336. SOLOMON-BLACKBURN, R., NIKAN, J. and BARKER, H. 2008, Mechanism of strong resistance to Potato leafroll virus infection in a clone of potato (Solanum tuberosum). Annals of Applied Biology, No 152: 339–347. STEVENSON, W. R., LORIA, R., FRANC, G. D. and WEINGARTNER, D. O. 2001. Compendium of potato diseases. St. Paul, Minnesota, USA: APS press. 125 p. VALKONEN, J .P. T. 1994. Natural genes and mechanism for resistance to viruses in cultivated and wild potato species (Solanumspp.) plant Breeding, No 112: 1-16. WILSON, C. R. and JONES, R .A. C. 1993. Evaluation of resistance to Potato leafroll virus selected potato cultivars under field condition. Australian Journal of Experimental Agriculture, No 33: 83-90.
| ||
|
آمار تعداد مشاهده مقاله: 1,256 تعداد دریافت فایل اصل مقاله: 394 |
||