| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,139 |
| تعداد مقالات | 34,918 |
| تعداد مشاهده مقاله | 50,383,128 |
| تعداد دریافت فایل اصل مقاله | 90,276,516 |
Molecular Detection of Chlamydia felis in Cats in Ahvaz, Iran | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 74، شماره 2، شهریور 2019، صفحه 119-126 اصل مقاله (227.9 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.116617.1172 | ||
| نویسندگان | ||
| M. Barimani؛ B. Mosallanejad* ؛ M. Ghorbanpoor؛ S. Esmaeilzadeh | ||
| Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| چکیده | ||
| Chlamydiae are obligate generally Gram-negative intracellular parasites with bacterial characteristics, including a cell wall, DNA, and RNA. They have a worldwide distribution in different animal species. Chlamydia felis (C. felis) is an important agent with zoonotic susceptibility often isolated from cats with chronic conjunctivitis. The aim of the present survey aimed to determine the molecular occurrence of C. felis in cats in Ahvaz, Iran. In this regard, a total of 152 cats (126 households and 26 feral) were included in the current study. After recording their history information, two swabs were taken from the oropharyngeal cavity and eye conjunctiva of the investigated cats. The extraction of DNA was followed by PCR targeting the pmp gene of C. Felis. In the next step, the positive samples were sequenced based on the Gene Bank. Out of 152 samples, 35 (23.03%) were positive using polymerase chain reaction technique (95% CI: 16.30-29.70). Regarding infection with Chlamydiosis, the obtained results showed a significant difference between cats suffering from ocular or respiratory diseases (44.64%; 25 out of 56) and the healthy ones (10.42%; 10 out of 96; P=0.01). The prevalence of infection was significantly higher in cats younger than 1 year (34.12%; 29 out of 85), compared to those older than 1 year (8.96%; 6 out of 67; P=0.02). No significant difference was noted in terms of gender (25.45% in males and 21.65% in females), breed (23.81% in DSH and 19.23% in Persian), and lifestyle (22.22% companions [28 out of 126] and 26.92% ferals [7 out of 26]; P>0.05). It can be concluded that a significant number of cats are infected with C. felis in Ahvaz. The use of molecular tests, such as PCR, has revolutionized the diagnosis of chlamydial infections. | ||
| کلیدواژهها | ||
| Chlamydia felis؛ Chlamydiosis؛ PCR؛ Ahvaz؛ Cat | ||
| مراجع | ||
|
Bannasch, M.J., Foley, J.E., 2005. Epidemiologic evaluation of multiple respiratory pathogens in cats in animal shelters. J Feline Med Surg 7, 109-119.
Bommana, S., Pokinghorne, A., 2019. Mini Review: Antimicrobial control of chlamydial infections in Animals: Current practices and issues. Front Microbiol 10, 1-9.
Borel, N., Polkinghorne, A., Pospischil, A., 2018. A review on chlamydial diseases in animals: still a challenge for pathologists? Vet pathol 55, 374-390.
Cantekin, Z., Solmaz, H., Altug, N., Ozmen, G.O., 2014. Development of pmp Gene-Specific PCR Assay with A Host Specific Internal Control for Chlamydophila felis. Thai J Vet Med 44, 469.
Halánová, M., Sulinova, Z., Čisláková, L., Trbolova, A., Páleník, Ľ., Weissova, T., et al., 2011. Chlamydophila felis in Cats–Are the Stray Cats Dangerous Source of Infection? Zoonoses Public Hlth 58, 519-522.
Hartmann, A.D., Hawley, J., Werckenthin, C., Lappin, M.R., Hartmann, K., 2010. Detection of bacterial and viral organisms from the conjunctiva of cats with conjunctivitis and upper respiratory tract disease. J Feline Med Surg 12, 775-782.
Helps, C., Reeves, N., Egan, K., Howard, P., Harbour, D., 2003. Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis. J Clin Microbiol 41, 2734-2736.
Johnson, F., 1984. Isolation of Chlamydia psittaci from nasal and conjunctival exudate of a domestic cat. Vet Rec 114, 342-344.
Kang, B.-T., Park, H.-M., 2008. Prevalence of feline herpesvirus 1, feline calicivirus and Chlamydophila felis in clinically normal cats at a Korean animal shelter. J Vet Sci 9, 207-209.
Maazi, N., Jamshidi, S., Kayhani, P., Momtaz, H., 2016. Occurrence of Chlamydophila felis, feline herpesvirus 1 and calcivirus in domestic cats of Iran. Iran J Microbiol 8, 312.
Masubuchi, K., Nosaka, H., Iwamoto, K., Kokubu, T., Yamanaka, M., Shimizu, Y., 2002. Experimental infection of cats with Chlamydophila felis. J Vet Med Sci 64, 1165-1168.
Millán, J., Rodríguez, A., 2009. A serological survey of common feline pathogens in free-living European wildcats (Felis silvestris) in central Spain. Eur J Wildlife Res 55, 285-291.
Momtaz, H., Alirezaie, M., Keyhani, P., 2014. Molecular detection of Chlamydophila felis in ocular discharge of domestic cats in Tehran & Isfahan. Biological Journal of Microorganism 3, 49-57.
Okuda, H., Ohya, K., Shiota, Y., Kato, H., Fukushi, H., 2011. Detection of Chlamydophila psittaci by using SYBR green real-time PCR. J Vet Med Sci 73, 249-254.
Rampazzo, A., Appino, S., Pregel, P., Tarducci, A., Zini, E., Biolatti, B., 2003. Prevalence of Chlamydophila felis and feline herpesvirus 1 in cats with conjunctivitis in northern Italy. J Vet Intern Med 17, 799-807.
Sandmeyer, L.S., Waldner, C.L., Bauer, B.S., Wen, X., Bienzle, D., 2010. Comparison of polymerase chain reaction tests for diagnosis of feline herpesvirus, Chlamydophila felis, and Mycoplasma spp. infection in cats with ocular disease in Canada. Canadian Vet J 51, 629-633.
Sibitz, C., Rudnay, E.C., Wabnegger, L., Spergser, J., Apfalter, P., Nell, B., 2011. Detection of Chlamydophila pneumoniae in cats with conjunctivitis. Vet Ophthalmol 14, 67-74.
Sykes, J.E., 2005. Feline chlamydiosis. Clin Tech Small An P 20, 129-134.
Sykes, J.E., Allen, J.L., Studdert, V.P., Browning, G.F., 2001. Detection of feline calicivirus, feline herpesvirus 1 and Chlamydia psittaci mucosal swabs by multiplex RT-PCR/PCR. Vet Microbiol 81, 95-108.
Sykes, J.E., Greene, C.E., 2012. Chlamydial infections. In: Greene, C.E. (Ed.), Infectious Diseases of the Dog and Cat, Missouri, St. Louis, pp. 270-276.
Wu, S.-M., Huang, S.-Y., Xu, M.-J., Zhou, D.-H., Song, H.Q., Zhu, X.-Q., 2013. Chlamydia felis exposure in companion dogs and cats in Lanzhou, China: a public health concern. BMC Vet Res 9, 104. | ||
|
آمار تعداد مشاهده مقاله: 3,108 تعداد دریافت فایل اصل مقاله: 3,875 |
||