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Development of an Indirect Enzyme-linked Immunosorbent Assay to Detect Antibodies against Serotype A2013 of Foot and Mouth Disease Virus in Cattle | ||
| Archives of Razi Institute | ||
| مقاله 7، دوره 74، شماره 2، شهریور 2019، صفحه 143-155 اصل مقاله (542.28 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.116378.1164 | ||
| نویسندگان | ||
| F. Malekdar* 1؛ H. Mahravani2؛ A. Sedigh3؛ M. Akbarzadegan2 | ||
| 1Department of Foot and Mouth Disease, Razi Vaccine and Serum Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran | ||
| 2Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran | ||
| 3Department of Human Viral Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran | ||
| چکیده | ||
| Foot and mouth disease (FMD) is a contagious animal disease that causes irreparable damage to the economy of countries, including Iran in which this disease is a native one. Among the ways to combat FMD are vaccination and slaughter. Due to the specific situation of Iran, it is not possible to kill infected animals. Therefore, vaccination is the most important way to fight this disease. Serum neutralization test (SNT) and enzyme-linked immunosorbent assay (ELISA) are two main methods to evaluate the safety and calculate antibody titer. In this study, an indirect ELISA test was developed based on the coating of a complete viral particle (140s) which made it possible to determine antibody. In addition, serotype and viral type were determined without the need for time-consuming and complex molecular tasks, including gene expression. Moreover, in case of a new epidemic, a new epidemic condition can be detected using a serum antibody method. However, the coating of the complete viral particle leads to virus purification as well as the conjugated anti-immunoglobulin antibody testing of the same animal. In this study, the SNT was used as a gold standard test to determine the serum antibody level and compare its results with indirect ELISA method to determine the sensitivity and specificity of the indirect ELISA. To measure the anti-virus antibody rate of FMD (type A2013) through receiver operating characteristic analysis with 100% sensitivity and the specificity of 90%, the routine formulas were utilized using 100 % and 82%sensitivity and specificity, respectively. In this study, the cutoff value for the optical density was obtained at 0.3 and there was a significant difference between the vaccinated animals and the unvaccinated ones in terms of antibody level against the A2013 type. This indicates the correctness of the test and the accurate and proportional antibody detection against the understudy viral types of FMD. | ||
| کلیدواژه‌ها | ||
| Antibody؛ Cattle؛ Cut off؛ Enzyme-linked immunosorbent assay؛ Foot and Mouth disease؛ Serum neutralization test | ||
| عنوان مقاله [English] | ||
| Development of an Indirect Enzyme-linked Immunosorbent Assay to Detect Antibodies against Serotype A2013 of Foot and Mouth Disease Virus in Cattle | ||
| مراجع | ||
|
Abdollahi, D., Meshkat , M., 2004. Foot and Mouth Disease status in The Islamic Republic of Iran. Country report, Iran veterinary organization, p. Appendix 9.
Bachrach , H., Moore, D., McKercher, P., Polatnick, J., 1975. Immune and antibody responses to an isolated capsid protein of foot-and-mouth disease virus. J Immunol.
Dagenais , P., Desprez, B., Alber, t., E, E., 1994. Direct Covalent Attachment of Small Peptide Antigens to Enzyme-Linked Immunosorbent Assay Plates Using Radiation and Carbodiimide Activation. Anal Biochem 222, Pages 149-155.
Emam, M., 1996. Studies on the effect of maternal immunity antibodies on the early aged vaccination with FMD vaccine. Ph. D. Thesis Faculty of Vetrinary Zagazig University, Infectious Diseases.
Fabrizio, C., Marradi, M., 2013. High Sensitive Detection of Carbohydrate Binding Proteins in an ELISA-Solid Phase Assay Based on Multivalent Glyconanoparticles. plos.
Hemmings, 2009. The importance of regular inlab checking of pipettes. Biohit. PDF http://www.fisher.co.uk/techzone/ pdfs/pipetting_techniques.pdf
Jamal, S., Belsham , G., 2013 Foot-and-mouth disease: past, present and future. Vet Res.
Jitendra, K.B., Sarita, J., Jajati, K., Moha, P., Punam, B., Bramhadev, P., 2013. Detection of antibodies specific for foot-and-mouth disease virus infection using indirect ELISA based on recombinant nonstructural protein 2B Arch Viral
Malekdar, F., Mahravani, H., 2017. Designing Indirect ELISA Test for Detection of Antibodies against Serotype A2013 & O2010 of Foot and Mouth Disease (FMD) Virus in Cattle. FMD department. Razi Vaccine and Serum Research Institute M.Sc., Karaj, pp. 45-120.
Mannonen, S., Nieminen, P., Kaasinen, J., Andersin, 2006. Raising the standard of mechanical pipetting. Biohit.PDF.
Mannonen, S., Tiusanen, T., Suovaniemi, O., 2000. Major sources of error of air displacement pipettors Biohit.PDF
Ming , Y., Tim , S., Satya, P., Kate, H., Lauro , V., Alfonso, C., et al., 2015. Development of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against the 3B Protein of Foot-and-Mouth Disease Virus. Clin Vaccine Immunol.
Oh, J., Song, D., Yang , J., Song, J., Moon, H., Kim, T., et al., 2005. Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection. J Vet Sci. , 349-352.
Oh, J.S., Song, D.S., Yang, J.S., 2005. Comparison of an enzyme-linked immunosorbent assay with serum neutralization test Veterinary Science, 349–352.
Schultheiss, O.C., Stanton, S.J., Jones, E., Beer, J.S., 2009. Assessment of salivary hormones. Methods in Social Neuroscience New York: Guilford Press.
Shojaee, M., Zibaei, S., January 2017. Purification of 146s Antigen of Foot-and-Mouth Disease (FMD) Virus Serotypes Aby Using the Sucrose Gradient Procedure. Int J Infect.
Tekleghiorghis, T., Weerdmeester , k., 2014. Comparison of Test Methodologies for Foot-and-Mouth Disease Virus Serotype A Vaccine Matching.Clin Vaccine Immunol, 674–683.
Yang , M., Parida , S., Salo, T., Hole, K., Velazquez-Salinas, L., Clavijo , A., 2015. Development of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against the 3B Protein of Foot-and-Mouth Disease Virus. Clin Vaccine Immunol., 389-397. | ||
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