Analysis and comparison of the relation between the resistance pattern to erythromycin and tetracycline, and the prevalence of the super-antigens coding enterotoxins A and B in Staphylococcus aureus isolated from broiler poultry in Ilam province

Mojahed Asl, Leila; Saleki, Khalil; Nemati, Mostafa

doi:10.22092/ari.2018.116231.1161

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	Analysis and comparison of the relation between the resistance pattern to erythromycin and tetracycline, and the prevalence of the super-antigens coding enterotoxins A and B in Staphylococcus aureus isolated from broiler poultry in Ilam province
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده ، انتشار آنلاین از تاریخ 26 اسفند 1397
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2018.116231.1161
نویسندگان
Leila Mojahed Asl¹؛ Khalil Saleki ²؛ Mostafa Nemati³
¹Para Veterinary Medicine School, Ilam University
²Associate Professor, Department of Microbiology, School of Para Veterinary Medicine , Ilam University
³Department Microbiology, School of Para Veterinary Medicine, Ilam University
چکیده
Abstract: Introduction and objective: This study is performed to trace the genes coding enterotoxins A and B in Staphylococcus aureus isolated from the clinic and poultry slaughterhouse, also analyze the relation between the prevalence of these genes and resistance to erythromycin and tetracycline antibiotics. Materials and methods: This study was performed from October 2015 to December 2016. 200 samples from the poultry noses and cloaca from broiler poultry farms in Ilam province (including 150 samples isolated from the slaughterhouse, and 50 samples isolated from the clinic) were isolated for separating Staphylococcus aureus. After bacterial culture and confirmation of biochemical tests, the samples were evaluated for identification of Staphylococcus aureus, and the resistance pattern against antibiotics regarding the presence of femA, tets, ermb, sea, and seb genes, using the disk diffusion method and the PCR test. Findings: Of 200 samples tested, 112 Staphylococcus aureus strains (56%) were identified, of which 91 strains were associated with the poultry slaughterhouse and 21 strains were associated with the clinic; and all were identified using biochemical tests. After detecting the femA gene, the exclusive gene for identifying Staphylococcus aureus strain, 100 strains (50%) were confirmed to be contaminated with this bacterium. Of these, 46% possessed the gene coding enterotoxin A, 14% possessed the gene coding enterotoxin B, and 5% possessed both genes. Performing antibiotic tests showed that 85% of the strains examined were resistant to erythromycin, and 86% to tetracycline. Conclusion: In the present study, analysis using the Quick calcs software showed that the strains resistant to these two antibiotics possessing the sea gene were more prevalent than those possessing seb genes, in the samples isolated from the poultry slaughterhouse. This comparison shows that during the short period of growth of broiler poultry farms, resistant strains had the ability to proliferate the sea gene among the herd, and its prevalence increased until the stage of reaching the slaughterhouse. This study shows that the relation between the genes resistant to erythromycin and tetracycline antibiotics and the sea gene, is close and significant.
کلیدواژه‌ها
Staphylococcus aureus؛ Poultry؛ enterotoxins A&B


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Analysis and comparison of the relation between the resistance pattern to erythromycin and tetracycline, and the prevalence of the super-antigens coding enterotoxins A and B in Staphylococcus aureus isolated from broiler poultry in Ilam province