| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,873 |
| تعداد مقالات | 32,558 |
| تعداد مشاهده مقاله | 42,483,005 |
| تعداد دریافت فایل اصل مقاله | 19,248,094 |
Comparison of Relation between Resistance Pattern to Erythromycin and Tetracycline and the Prevalence of Superantigens Coding Enterotoxins A and B in Staphylococcus aureus Isolated from Broiler Poultry in Ilam, Iran | ||
| Archives of Razi Institute | ||
| مقاله 8، دوره 74، شماره 2، شهریور 2019، صفحه 157-164 اصل مقاله (562.53 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.116231.1161 | ||
| نویسندگان | ||
| L. Mojahed Asl؛ K. Saleki* ؛ M. Nemati | ||
| Department of Microbiology, School of Para Veterinary Medicine, Ilam University, Ilam, Iran | ||
| چکیده | ||
| Staphylococcus aureus is a gram-positive coccus that, in specific conditions, is able to generate various diseases. By secreting different enterotoxins, this bacterium prepares the settings to attack the host; among these, enterotoxins A and B play the most important roles in food poisoning. This study was performed to trace the genes coding enterotoxins A and B in Staphylococcus aureus isolated from the clinic and poultry slaughterhouse. In addition, the present study analyzed the relation between the prevalence of these genes and resistance to erythromycin and tetracycline. This study was performed from October 2015 to December 2016. A total of 200 samples of noses and cloaca from broiler poultry farms in Ilam, Iran, were collected, including 150 samples from the slaughterhouse and 50 samples from the clinic isolated for separating Staphylococcus aureus. After bacterial culture and confirmation of biochemical tests, the samples were evaluated for the identification of Staphylococcus aureus and the resistance pattern to antibiotics regarding the presence of femA, tets, ermb, sea, and seb genes using the disk diffusion method and polymerase chain reaction test. Out of 200 tested samples, 112 strains of Staphylococcus aureus (56%) were identified from which 91 and 21 strains were associated with the poultry slaughterhouse and clinic, respectively, and all the samples were identified using biochemical tests. After the detection of femA gene as an exclusive gene for the identification of Staphylococcus aureus strain, 100 strains (50%) were confirmed to be contaminated with this bacterium. Out of 100 strains, 46%, 14%, and 5% possessed the genes coding enterotoxin A, the genes coding enterotoxin B, and both genes, respectively. The results of antibiotic tests showed that 85% and 86% of the examined strains were resistant to erythromycin and tetracycline, respectively. In the present study, the analysis performed using QuickCalcs software showed that the strains resistant to these two antibiotics possessing the sea gene were more prevalent than those possessing seb genes in the samples isolated from the poultry slaughterhouse. This comparison revealed that during the short period of broiler poultry farms growth, resistant strains were able to proliferate sea gene among the herd, and its prevalence increased until reaching into the slaughterhouse. This study showed that the relation between the genes resistant to erythromycin and tetracycline and the sea gene was close and significant. | ||
| کلیدواژه‌ها | ||
| Staphylococcus aureus؛ Poultry؛ Enterotoxins A & B | ||
| مراجع | ||
|
Alibayov, B., Zdeňková, K., Purkrtová, S., Demnerová, K., Karpíšková, R., 2014. Detection of some phenotypic and genotypic characteristics of staphylococcus aureus isolated from food items in the Czech Republic. Ann Microbiol 64, 1587-1596.
Barati, B., Saadati, M., Bahmani, M.K., 2006. Isolation and Detection of Enterotoxigenic Staphylococcus aureus type A by multiplex PCR. J Military Med 8, 28-119.
Bergdoll, M., 1983. Enterotoxins. Staphylococci and Staphylococcal infections, Academic Press, London.
Bystron, J., Molenda, J., Bania, J., Kosek-Paszkowska, K., Czerw, M., 2005. Occurrence of enterotoxigenic strains of Staphylococcus aureus in raw poultry meat. Pol J Vet Sci 8, 37-40.
Casman, E.P., Bennett, R.W., Dorsey, A.E., Issa, J.A., 1967. Identification of a fourth staphylococcal enterotoxin, enterotoxin D. J Bacteriol 94, 1875-1882.
Chiang, Y.C., Liao, W.W., Fan, C.M., Pai, W.Y., Chiou, C.S., Tsen, H.Y., 2008. PCR detection of Staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan. Int J Food Microbiol 121, 66-73.
Cui, J.C., Zhang, B.J., Lin, Y.C., Wang, Q.K., Qian, A.D., Nakane, A., et al., 2010. Protective effect of glutathione S-transferase-fused mutant staphylococcal enterotoxin C against Staphylococcus aureus-induced bovine mastitis. Vet Immunol Immunopathol 135, 64-70.
Eshraghi, S., Salehipour, Z., Pourmand, M., Rahimi, F.A., Zahraei, S.M.T., Agha, A.S., et al., 2009. Prevalence of tst, entC, entA and entA/C genes in staphylococcus aureus strains isolated from different foods.
Feizi, A., Nazeri, M., Pilevar, A., 2012. Isolation of Staphylococcus spp. genera from broiler breeder flocks in East Azerbaijan Province of Iran: Prevalence and antimicrobial susceptibility.
Ifesan, B.O., Voravuthikunchai, S.P., 2009. Effect of Eleutherine americana Merr. extract on enzymatic activity and enterotoxin production of Staphylococcus aureus in broth and cooked pork. Foodborne Pathog Dis 6, 699-704.
Imani-Fooladi, A., Riazipour, M., Sattari, M., 2009. Comparison of molecular and serologic identification of Staphylococcus producing enterotoxins from dairy products traditionally. J Shahrekord U Med Sci 11th period 4, 19-26.
Khakpoor, M., Ezzati, M., Mahmoodi, K., Khalaji Pirbaluti, M., Khaksar, R., 2013. Prevalence of Coagulase-positive Staphylococcus aureus in local Cheese in West Azerbaijan with culture and PCR method. Iranian Journal of Nutrition Sciences & Food Technology 7, 238-242.
Lim, S.K., Joo, Y.S., Moon, J., Lee, A.R., Nam, H.M., Wee, S.H., et al., 2004. Molecular typing of enterotoxigenic Staphylococcus aureus isolated from bovine mastitis in Korea. J Vet Med Sci 66, 581-584.
Mashouf, R.Y., Hosseini, S.M., Mousavi, S.M., Arabestani, M.R., 2015. Prevalence of Enterotoxin Genes and Antibacterial Susceptibility Pattern of Staphylococcus aureus Strains Isolated from Animal Originated Foods in West of Iran. Oman Med J 30, 283-290.
Parsaeimehr, M., Basti, A.A., Radmehr, B., Misaghi, A., Abbasifar, A., KarimG., et al., 2010. Effect of Zataria multiflora Boiss. essential oil, nisin, and their combination on the production of enterotoxin C and alpha-hemolysin by Staphylococcus aureus. Foodborne Pathog Dis 7, 299-305.
Reischl, U., Linde, H.J., Metz, M., Leppmeier, B., Lehn, N.,2000. Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR. J Clin Microbiol 38, 2429-2433.
Rodriguez, M., Nunez, F., Cordoba, J.J., Bermudez, E., Asensio, M.A., 1996. Gram-positive, catalase-positive cocci from dry cured Iberian ham and their enterotoxigenic potential. Appl Environ Microbiol 62, 1897-1902. Saadati, M., Barati, B., Doroudian, M., Shirzad, H., Hashemi, M., Hosseini, S.M., et al., 2008. Detection of sea, sec and seq genes in Staphylococcus aureus nasal sampling acquiring from healthy carriers in Tehran province, Iran; by multiplex PCR. J Paramed Sci 2, 34-40.
Salari-Sharif, A., Sattari, M., Moradi, M., Shahrokhabadi, R., 2012. Detecting enterotoxin A and B genes of Staphylococcus bacteria in samples of clinical patients visiting treatment sources of Kerman and Rafsanjan using the molecular method. J Rafsanjan U Med Sci 11, 128-136.
Shapury, R., Rahnema, M., Eghbal Zadeh, S., 2009. The Prevalence of Salmonella serotypes in poultry and eggs, and determine antibiotic susceptibility of them in the Zanajan city. Q J Anim Phisiol Dev 3, 63-71.
Shekarforoush, S.S., Kiaie, S.M.M., Karim, G., Razavi Rohani, S.M., Rokni, N., Abbasvali, M., 2013. Study on the overview on foodborne bacteria in food with animal origin in Iran; Part four: Poultry and egg. J Food Hyg 3, 45-64.
Wang, S.-J., Chow, L.-W., Wu, M.-J., 2002. Multiplex PCR for the simultaneous detection of the SEA, SEB, SEC, SED and SEE genes of enterotoxigenic staphylococcus aureus. | ||
|
آمار تعداد مشاهده مقاله: 1,911 تعداد دریافت فایل اصل مقاله: 535 |
||