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قارچهای اندوفیت جداشده از درختان بلوط (Quercus brantii Lindl و Quercus infectoria Oliv) | ||
| تحقیقات حمایت و حفاظت جنگلها و مراتع ایران | ||
| مقاله 5، دوره 16، شماره 2 - شماره پیاپی 32، اسفند 1397، صفحه 171-189 اصل مقاله (673.85 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijfrpr.2019.118690 | ||
| نویسندگان | ||
| آسو حاجی زاده1؛ جهانشیر امینی* 2؛ جعفر عبدالله زاده2 | ||
| 1فارغ التحصیل کارشناسی ارشد، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه کردستان، کردستان، ایران | ||
| 2دانشیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه کردستان، کردستان، ایران | ||
| چکیده | ||
| DOR: 98.1000/1735-0859.1397.16.171.32.2.1602.41 قارچهای اندوفیت بخشی از اجزای مهم تنوع زیستی مرتبط با گیاهان هستند و قادر بوده تمام بافتهای داخلی گیاهان را کلنه کرده و سبب بهبود سلامت گیاه شوند. آنها میتوانند با بیمارگرهای گیاهان برای فضا و غذا از نظر اکولوژیکی رقابت کرده و موجب کاهش بیماری در گیاهان شوند. بهمنظور شناسایی قارچهای اندوفیت از برگ، تنه و پوست شاخههای سالم درختان بلوط گونههای Quercus brantii Lindlو Quercus infectoria Olivدر استان کردستان از مناطق بانه، کامیاران و مریوان نمونه‌برداری بهصورت تصادفی انجام شد. نمونه‌ها در آزمایشگاه به قطعات نیم تا یک سانتیمتری تقسیم و پس از ضدعفونی با هیپوکلرید سدیم 5% و اتانول 70% با آب مقطر سترون شستشو داده و پس از خشک کردن روی کاغذ صافی سترون روی محیط سیبزمینی- دکستروز – آگار حاوی آنتی‌بیوتیک داکسیسایکلین (150 میلیگرم در لیتر) در دمای 25 درجه سانتیگراد کشت شد. شناسایی گونه‌ها براساس خصوصیات ریختشناختی و توالی ناحیه ITS-rDNA انجام گردید. بر این اساس از 75 جدایه، گونههای Peyronellaea glomerata (Corda)، Truncatella angustata (Pers.) Hughes، Ulocladium botrytis (Preuss)، Microsphaeropsis olivacea (Bonord.)، Chaetomium subaffine (Sergeeva)، Trichoderma citrinoviride (Bissett) و Trichoderma atroviride (P. Karst)` شناسایی شدند. دو گونه Chaetomium subaffine و Ulocladium botrytisبیشترین فراوانی را در بین قارچهای جدا شده از بافتهای مورد مطالعه به خود اختصاص دادند. علاوه بر این، بیشترین جدایههای اندوفیت بهترتیب از بافت ساقه و تنه درختان بلوط گونه Q. brantiiجدا شد. | ||
| کلیدواژه‌ها | ||
| بلوط؛ مطالعات فیلوژنتیکی؛ ITS؛ Truncatella؛ Peyronellaea | ||
| عنوان مقاله [English] | ||
| The endophytic fungi isolated from oak trees (Quercus brantii Lindl and Quercus infectoria Oliv.) | ||
| نویسندگان [English] | ||
| A. Hajizadeh1؛ Jahanshir Amini2؛ J. Abdollahzadeh2 | ||
| 2Dep. Plant Protection, Agriculture Faculty, University of Kurdistan, Sanandaj, Iran | ||
| چکیده [English] | ||
| Fungal endophytes are a known part of biodiversity associated with plants. They can colonize all parts of the host plant tissues and play an important role in enhancing plant health. Also, they can compete with the pathogens for the same niches and thereby reduce disease severity in plant. In order to identify endophytic fungi of oak trees, healthy plant samples including twigs, stems and leaves were collected from forest regions of Kurdistan province. Samples were cut into 2-cm-long segments. Then, pieces of samples were surface sterilized in 5% sodium hypochlorite and 70% ethanol and rinsed with sterile water three times. Then, samples blotted dry on sterilized filter paper and were cultured on Potato Dextrose Agar (PDA) medium amended with Doxycycline antibiotic 25 oC. A total number of 75 isolates were obtained. Based on morphological characteristics and ITS- rDNA regions sequence data, seven different fungal species including Peyronellaea glomerata, Truncatella angustata, Ulocladium botrytis, Microsphaeropsis olivacea, Chaetomium subaffine, Trichoderma citrinoviride and Trichoderma atroviride were identified as endophytic fungi of oak trees in Kurdistan Province. Chaetomium subaffine and Ulocladium botrytis were among the fungi showing the highest colonization frequency (CF%) in all the tissues analysed. The number of isolated taxa was greater in the stem and trunk of oak trees respectively than those in the other organs analyzed. | ||
| کلیدواژه‌ها [English] | ||
| Oak, fungal endophytes, Truncatella, Peyronellaea | ||
| مراجع | ||
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