| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,829 |
| تعداد مقالات | 32,098 |
| تعداد مشاهده مقاله | 40,864,102 |
| تعداد دریافت فایل اصل مقاله | 18,113,726 |
بررسی تاثیر عصاره گیاه آنیسون(Pimpinella anisum L.) و گلپر(Heracleum persicum) در مقایسه با اکسی‌تتراسایکلین و پروبیوتیک پریمالاک بر پاسخ ایمنی، جمعیت میکروبی ایلئوم و خصوصیات مورفولوژیکی تهی‌روده جوجه‌های گوشتی | ||
| علوم دامی | ||
| مقاله 3، دوره 31، شماره 121، اسفند 1397، صفحه 27-38 اصل مقاله (1.85 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2018.115546.1538 | ||
| نویسندگان | ||
| مجید همتی* 1؛ جعفر فخرایی2؛ اکبر یعقوبفر3؛ حسین منصوری یاراحمدی4 | ||
| 1دانشجوی دکتری تغذیه دام دانشگاه آزاد اسلامی واحد اراک | ||
| 2استادیار گروه علوم دامی دانشگاه آزاد اسلامی واحد اراک. | ||
| 3استاد پژوهشی موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 4استادیار گروه علوم دامی دانشگاه آزاد اسلامی واحد اراک | ||
| چکیده | ||
| هدف از این آزمایش تعیین اثرات عصاره‌های گیاهان آنیسون و گلپر(mg/kg 200) در مقایسه با آنتی‌بیوتیک اکسی‌تتراسایکلین(mg/kg 200) و پروبیوتیک پریمالاک(mg/kg 100)بر پاسخ ایمنی، جمعیت میکروبی ایلئوم و خصوصیات مورفولوژیکی تهی‌روده جوجه‌های گوشتی بود. تعداد 400 قطعه جوجه گوشتی سویه راس 308 در قالب طرح کاملاً تصادفی به پنج تیمار آزمایشی اختصاص داده شدند. هر تیمار شامل 4 تکرار و هر تکرار دارای 20 قطعه جوجه یک روزه بود. ارزیابی پاسخ ایمنی جوجه‌ها به کمک تزریق سوسپانسیون 5 درصد گلبول قرمز گوسفند(SRBC)، بررسی جمعیت میکروبی روده با برداشتن محتویات ایلئوم پرنده‌ها و بررسی خصوصیات مورفولوژیکی روده با بریدن قسمت میانی تهی‌روده پس از کشتار صورت گرفت. نتایج این مطالعه نشان دادند که تیمارهای آزمایشی تاثیر معنی‌داری بر عیار آنتی‌بادی علیه SRBC نداشتند. فراوانی هتروفیل‌ها و نسبت هتروفیل به لنفوسیت، تحت تاثیر تیمارهای آزمایشی عصاره گیاهان آنیسون و گلپر کاهش معنی‌داری نشان دادند(05/0>P). جمعیت کل باکتری‌های هوازی، اشریشیاکلی و کلی‌فرم‌ها تحت تاثیر عصاره گیاه گلپر کاهش معنی‌داری داشتند(05/0>P). کلیه تیمارهای حاوی افزودنی در مقایسه با تیمار شاهد سبب افزایش معنی‌داری در ارتفاع پرزهای تهی‌روده شدند(05/0>P). نسبت طول پرز به عمق کریپت در پرزهای تهی‌روده توسط تیمارهای حاوی عصاره گیاه آنیسون و گلپر به طور معنی‌داری افزایش یافت(05/0>P). به طور کلی نتایج حاصل از این آزمایش نشان دادند که استفاده از عصاره گیاه آنیسون و گلپر به میزان 200 میلی‌گرم در هر کیلوگرم جیره ممکن است باعث بهبود پاسخ ایمنی، کاهش جمعیت میکروبی مضر ایلئوم و بهبود خصوصیات مورفولوژیکی تهی‌روده جوجه های گوشتی شود. | ||
| کلیدواژه‌ها | ||
| عصاره آنیسون؛ عصاره گلپر؛ آنتی‌بیوتیک؛ پروبیوتیک؛ جوجه‌های گوشتی | ||
| عنوان مقاله [English] | ||
| Evaluation the effect of anise (Pimpinella anisum L.) and angelica (Heracleum persicum) extracts in comparison with oxytetracycline and probiotice Primalac® on immune response, ileum microflora and jejunal morphological characteristics of broilers | ||
| نویسندگان [English] | ||
| Majid Hemati1؛ Jafar Fakhraei2؛ Akbar Yaghobfar3؛ Hossein Mansoory Yarahmadi4 | ||
| 1Department of Animal Science, Deputy of Education, Agricultural and Natural Resources Research and Education Center of Safi Abad, Dezful, Khuzestan 64651-85347, IRAN. | ||
| 2Department of Animal science, Islamic Azad University- Arak branch, Arak, Iran | ||
| 33Animal Science Research Institute, Agricultural Research Education and Extension Organization | ||
| 4Department of Animal Science, College of Agriculture and Natural Resource, Arak Branch, Islamic Azad University, Arak | ||
| چکیده [English] | ||
| The aim of this experiment was to determine the effects of anise and angelica extracts (200 mg/kg) in comparison with antibiotics oxytetracycline (200 mg/kg) and probiotic Primalace® (100 mg/kg) on immune response, ileal microflora and jejunal morphological characteristics of broilers. A total of 400 Ross 308 broiler chicks were assigned in a completely randomized design to five experimental treatments. Each treatment consisted of 4 replicates and 20 one-day old chicks per replication. The evaluation of immune response of chicks was conducted by injecting 5% SRBC suspension, assessment of ileal microflora by removing the ileum contents of the birds and examination of jejunal morphological characteristics by cutting the middle part of the jejunum after slaughter. The results of this study showed that experimental treatments had no significant effect on antibody titer against SRBC. The frequency of heterophils and heterophile to lymphocyte ratio decreased significantly affected by anise and angelica extracts (P<0.05). The total population of aerobic bacteria, Escherichia coli and Coliform bacteria decreased significantly by angelica extract (P<0.05). All treatments containing additive caused significant increase in jejunum villus height when compared to control treatment (P<0.05). The villus height to crypt depth ratio in jejunal was significantly increased by anise and angelica extracts (P<0.05). Generally, the results of this experiment showed that the use of anise and angelica extracts by amount of 200 mg/kg diet may improve immune response, reduce the harmful ileal microflora and improve jejunal morphological characteristics of broiler chickens. | ||
| کلیدواژه‌ها [English] | ||
| Anise extract, Angelica extract, Antibiotic, Probiotic, broilers | ||
| مراجع | ||
|
Adibmoradi, M., Navidshad, B., Seifdavati, J. and Royan, M. (2006). Effect of dietary garlic meal on histological structure of small intestine in broiler chickens. Journal of Poultry Science. 43:378-383. Ainsworth, E.A. and Gillespie, K.M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols. 2:875-877. Al-Bayati, F.A. (2008). Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts. Journal of Ethnopharmacology. 116:403-406. Arora, R. Gupta, D., Chawla, R., Sagar, R., Sharma, A., Kumar, R., Prasad, J., Singh, S., Samanta, N. and Sharma, R.K. (2005). Radioprotection by plant products: present status and future prospects. Phytotherapy Research. 19: 1-22. Asgarpanah, J., Dadashzadeh Mehrabani, G., Ahmadi, S.M. and Safialdin-Ardebili, M. (2012). Chemistry, pharmacology and medicinal properties of Heracleum persicum Desf. ex fischer: A review. Journal of Medicinal Plants Research. 6:1813-1820. Ayfer-Atefi, D. and Erdogrul, Ö.T. (2003). Antimicrobial activities of various medicinal and commercial plant extracts. Turkish Journal of Biology. 27:157-162. Besharati-Seidani, A., Jabbari, A. and Yamini, Y. (2005). Headspace solvent microextraction: a very rapid method for identification of volatile components of Iranian Pimpinella anisum seed. Analytica Chimica Acta. 530:155-161. Bown, D. (1995). The royal horticultural society new encyclopedia of herbs and their uses (rhs). Dunfermline: DK. Bradley, G.L., Savage, T.F. and Timm, K.I. (1994). The effects of supplementing diets with Saccharomyces cerevisiae var. boulardii on male poult performance and ileal morphology. Poultry Science. 73:1766-1770. Cabuk, M., Bozkurt, M., Alçiçek, A., Akbaþ, Y. and Küçükyýlmaz, K. (2006). Effect of a herbal essential oil mixture on growth and internal organ weight of broilers from young and old breeder flocks. South African Journal of Animal Science. 36:135-141. Cheng, S., Rotschild, M.F. and Lamont, S.J. (1991). Estimates of quantitative genetic parameters of immunological traits in the chicken. Poultry Science. 70:2023-2027. Cook, R.H. and Bird, F.H. (1973). Duodenal villus area and epithelial cellular migration in conventional and germ-free chicks. Poultry Science. 52:2276-2280. Cummings, J.H. and Macfarlane, G.T. (1997). Role of intestinal bacteria in nutrient metabolism. Clinical Nutrition. 16:3-11. Farag, R., Daw, Z., Hewedi, F. and El-Baroty, G. (1989). Antimicrobial activity of some Egyptian spice essential oils. Journal of Food Protection. 52:665-667. Fekri Yazdi, F., Ghalamkari G.R., Toghiani, M., Modaresi, M. and Landy, N. (2014). Anise seed (Pimpinella anisum L.) as an alternative to antibiotic growth promoters on performance, carcass traits and immune responses in broiler chicks. Asian Pacific Journal of Tropical Disease. 4:447-451. Ferket, P., Parks, C. and Grimes, J. (2002). Benefits of dietary antibiotic and mannanoligosaccharide supplementation for poultry. Multi-State Poultry Meeting. May:14-16. Hemati, A., Azarnia, M. and Angaji, S.A. (2010). Medicinal effects of Heracleum persicum(Golpar). Middle East Journal of Scientific Research. 5:174-176. Heres, L., Wagenaar, J.A., Van Knapen, F. and Urlings, B.A. (2003). Passage of Salmonella through the crop and gizzard of broiler chickens fed with fermented liquid feed. Avian Pathology. 33:173-181. Hong, J.C., Steiner, T., Aufy, A. and Lien, T.F. (2012). Effects of supplemental essential oil on growth performance, lipid metabolites and immunity, intestinal characteristics, microbiota and carcass traits in broilers. Livestock Science. 144:253-262. Hosseini, S.A., Tabatabaei-Vakili, S., Mamouei, M., Sallary, S., and Zarei, M. (2016). Effect of different levels of Heracleum Persicum seed in diet on performance, immune system, antioxidant capacity, concentrations of estrogen and some blood parameters in broiler chickens. Animal Sciences Journal. 29:133-146. Jafarzadeh, L., Sadeghi, M., Behzadian, M. and Rafieian-Kopaei, M. (2014). The Teratogenic and abortifacient effects of Heracleum Persicum hydroalcholic extract and its correlation with mothers’ estrogen and progesterone in balb/c mice. Journal Of Babol University Of Medical Sciences(JBUMS). 16:26-32. Jain, N. C. (1986). Schalm's Veterinary Hematology. 4th edition ed. pp. 240–255: Lea & Febiger. Philadelphia, USA. Jamroz, D., Wertelecki, T., Houszka, M. and Kamel, C. (2006). Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejunum walls in chicken. Journal of Animal Physiology and Animal Nutrition. 90:255-268. Juven, B., Kanner, J., Schved, F. and Weisslowicz, H. (1994). Factors that interact with the antibacterial action of thyme essential oil and its active constituents. Journal of Applied Bacteriology. 76:626-631. Khalaji, S., Zaghari, M., Hatami, K. H., Hedari-Dastjerdi, S., Lotfi, L. and Nazarian, H. (2011). Black cumin seeds, Artemisia leaves (Artemisia sieberi), and Camellia L. plant extract as phytogenic products in broiler diets and their effects on performance, blood constituents, immunity, and cecal microbial population. Poultry Science. 90: 2500-2510. Kossah, R., Nsabimana, C., Zhang, H. and Chen, W. (2010). Optimization of extraction of polyphenols from Syrian sumac (Rhus coriaria L.) and Chinese sumac (Rhus typhina L.) fruits. Research Journal of Phytochemistry. 4:146-153. Langhout, P. (2000). New additives for broiler chickens. World Poultry. 16:22-27. Li, Y.L. (1991). Culture Medium Manual (Changchun, china, jilin science and technology Press). Mathivanan, R. and Kalaiarasi, K. (2007). Panchagavya and andrographis panculata as alternatives to antibiotic growth promoters on hematological, serum biochemical parameters and immune status of broilers. Poultry Science. 44:198-204. Mc-Manus, J.F.A. (1984). Histological and histochemical uses of periodic acid. Stain Technology. 23:99-108. Miles, R.D., Butcher, G.D., Henry, P.R. and Littell, R.C. (2006). Effect of antibiotic growth promoters on broiler performance, intestinal growth parameters, and quantitative morphology. Poultry Science. 85:476-485. Mountzouris, K.C., Tsirtsikos, P., Paraskevas, V. and Fegeros, K. (2008). Evaluation of the effect of a phytogenic essential oil product on broiler performance and nutrient digestibility. Brisbane Australia.Pp. 444. Natt, M.P. and Herrick, C.A. (1952). A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poultry Science. 31:735-738. Nayebpor, M., Farhomand, P. and Hashemi, A. (2007). Effects of different levels of direct fed microbial (primalac) on growth performance and humoral immune response in broiler chickens. Journal of Animal and Veterinary Advances. 6:1308-1313. Nazemi, A., Hashemi, M., Khataminejad, M.R. and Pourshamsian, K. (2005). Antimicrobial activity of aqueous and methanol extracts of Heracoleum persicum. Medical Sciences. 15:91-94. Ouwehand, A.C., Tiihonen, K., Kettunen, H., Peuranen, S., Schulze, H. and Rautonen, N. (2010). In vitro effects of essential oils on potential pathogens and beneficial members of the normal microbiota. Veterinarni Medicina. 55:71-78. Pluske, J.R., Hampson, D.J. and Williams, I.H. (1997). Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livestock Production Science. 51:215-236. Rezaei, E., Tabatabaei-Vakili, S., Mirzadeh, K., Salary, S. and Zarei, M. (2016). Effect of anise seed (Pimpinella anisum L.) on performance, immune system, antioxidant activity and blood estrogen level in broiler chickens. Journal of Animal Production. 18:151-160. Ross 308 Broiler Performance Objectives. (2014). Aviagen North America, Huntsville, AL. Sakamoto, K., Hirose, H., Onizuka, A., Hayashi, M., Futamura, N. and Kawamura, Y. (2000). Quantitative study of changes in intestinal morphology and mucus gel on total parenteral nutrition in rats. Journal of Surgical Research. 94:99-106. SAS, I. (2002). SAS 9.1 Users Guide: Statistics. SAS Institute Inc. Cary, NC. Schulten, E.S., Yates, L.M. and Taylor, R.L. (2007). Antibody response against sheep red blood cells in lines congenic for major histocompatibility (B) complex recombinants. International Journal of Poultry Science. 6: 732-738. Sehm, J., Lindermayer, H., Dummer, C., Treutter, D. and Pfaffl, M.W. (2006). The influence of polyphenol rich apple pomace or red-wine pomace diet on the gut morphology in weaning piglets. Journal of Animal Physiology and Animal Nutrition. 91:289-296. Shirzadi, H. (2015). Effects of Syrian mesquite (Prosopis farcta) and Sumac (Rhuscoriaria L.) extracts on intestinal microbial population and ascites syndrome in broiler chickens. A Dissertation Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy (Ph. D.) in Poultry Nutrition.Tarbiat Modarres University. Shojaii, A. and Abdollahi-Fard, M. (2012). Review of pharmacological properties and chemical constituents of Pimpinella anisum. International Scholarly Research Network. 2012:8. Soltan, M., Shewita, R. and El-Katcha, M. (2008). Effect of dietary anise seeds supplementation on growth performance, immune response, carcass traits and some blood parameters of broiler chickens. International Journal of Poultry Science. 7:1078-1088. Stiles, J.C., Sparks, W. and Ronzio, R.A. (1995). The inhibition of Candida albicans by oregano. Journal of Applied Nutrition. 47:96-102. Svensson, E., Sinervo, B. and Comendant, T. (2002). Mechanistic and experimental analysis of condition and reproduction in a polymorphic lizard. Journal of Evolutionary Biology. 15:1034-1047. Trase, G. and Evance, W. (1996). Pharmacognosy. 14th ed., Bailliere Tindall., London. Wegmann, T.G. and Smithies, O. (1966). A simple hemagglutination system requiring small amounts of red cells and antibodies. Transfusion. 6:67-73. Xu, Z.R., Hu, C.H., Xia, M.S., Zhan, X.A. and Wang, M.Q. (2003). Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poultry Science. 82:1030-1036. | ||
|
آمار تعداد مشاهده مقاله: 561 تعداد دریافت فایل اصل مقاله: 431 |
||