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بررسی تاثیر عصاره گیاه آنیسون(Pimpinella anisum L.) و گلپر(Heracleum persicum) در مقایسه با اکسی‌تتراسایکلین و پروبیوتیک پریمالاک بر پاسخ ایمنی، جمعیت میکروبی ایلئوم و خصوصیات مورفولوژیکی تهی‌روده جوجه‌های گوشتی | ||
| علوم دامی | ||
| مقاله 3، دوره 31، شماره 121، اسفند 1397، صفحه 27-38 اصل مقاله (1.85 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2018.115546.1538 | ||
| نویسندگان | ||
| مجید همتی* 1؛ جعفر فخرایی2؛ اکبر یعقوبفر3؛ حسین منصوری یاراحمدی4 | ||
| 1دانشجوی دکتری تغذیه دام دانشگاه آزاد اسلامی واحد اراک | ||
| 2استادیار گروه علوم دامی دانشگاه آزاد اسلامی واحد اراک. | ||
| 3استاد پژوهشی موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 4استادیار گروه علوم دامی دانشگاه آزاد اسلامی واحد اراک | ||
| چکیده | ||
| هدف از این آزمایش تعیین اثرات عصاره‌های گیاهان آنیسون و گلپر(mg/kg 200) در مقایسه با آنتی‌بیوتیک اکسی‌تتراسایکلین(mg/kg 200) و پروبیوتیک پریمالاک(mg/kg 100)بر پاسخ ایمنی، جمعیت میکروبی ایلئوم و خصوصیات مورفولوژیکی تهی‌روده جوجه‌های گوشتی بود. تعداد 400 قطعه جوجه گوشتی سویه راس 308 در قالب طرح کاملاً تصادفی به پنج تیمار آزمایشی اختصاص داده شدند. هر تیمار شامل 4 تکرار و هر تکرار دارای 20 قطعه جوجه یک روزه بود. ارزیابی پاسخ ایمنی جوجه‌ها به کمک تزریق سوسپانسیون 5 درصد گلبول قرمز گوسفند(SRBC)، بررسی جمعیت میکروبی روده با برداشتن محتویات ایلئوم پرنده‌ها و بررسی خصوصیات مورفولوژیکی روده با بریدن قسمت میانی تهی‌روده پس از کشتار صورت گرفت. نتایج این مطالعه نشان دادند که تیمارهای آزمایشی تاثیر معنی‌داری بر عیار آنتی‌بادی علیه SRBC نداشتند. فراوانی هتروفیل‌ها و نسبت هتروفیل به لنفوسیت، تحت تاثیر تیمارهای آزمایشی عصاره گیاهان آنیسون و گلپر کاهش معنی‌داری نشان دادند(05/0>P). جمعیت کل باکتری‌های هوازی، اشریشیاکلی و کلی‌فرم‌ها تحت تاثیر عصاره گیاه گلپر کاهش معنی‌داری داشتند(05/0>P). کلیه تیمارهای حاوی افزودنی در مقایسه با تیمار شاهد سبب افزایش معنی‌داری در ارتفاع پرزهای تهی‌روده شدند(05/0>P). نسبت طول پرز به عمق کریپت در پرزهای تهی‌روده توسط تیمارهای حاوی عصاره گیاه آنیسون و گلپر به طور معنی‌داری افزایش یافت(05/0>P). به طور کلی نتایج حاصل از این آزمایش نشان دادند که استفاده از عصاره گیاه آنیسون و گلپر به میزان 200 میلی‌گرم در هر کیلوگرم جیره ممکن است باعث بهبود پاسخ ایمنی، کاهش جمعیت میکروبی مضر ایلئوم و بهبود خصوصیات مورفولوژیکی تهی‌روده جوجه های گوشتی شود. | ||
| کلیدواژه‌ها | ||
| عصاره آنیسون؛ عصاره گلپر؛ آنتی‌بیوتیک؛ پروبیوتیک؛ جوجه‌های گوشتی | ||
| عنوان مقاله [English] | ||
| Evaluation the effect of anise (Pimpinella anisum L.) and angelica (Heracleum persicum) extracts in comparison with oxytetracycline and probiotice Primalac® on immune response, ileum microflora and jejunal morphological characteristics of broilers | ||
| نویسندگان [English] | ||
| Majid Hemati1؛ Jafar Fakhraei2؛ Akbar Yaghobfar3؛ Hossein Mansoory Yarahmadi4 | ||
| 1Department of Animal Science, Deputy of Education, Agricultural and Natural Resources Research and Education Center of Safi Abad, Dezful, Khuzestan 64651-85347, IRAN. | ||
| 2Department of Animal science, Islamic Azad University- Arak branch, Arak, Iran | ||
| 33Animal Science Research Institute, Agricultural Research Education and Extension Organization | ||
| 4Department of Animal Science, College of Agriculture and Natural Resource, Arak Branch, Islamic Azad University, Arak | ||
| چکیده [English] | ||
| The aim of this experiment was to determine the effects of anise and angelica extracts (200 mg/kg) in comparison with antibiotics oxytetracycline (200 mg/kg) and probiotic Primalace® (100 mg/kg) on immune response, ileal microflora and jejunal morphological characteristics of broilers. A total of 400 Ross 308 broiler chicks were assigned in a completely randomized design to five experimental treatments. Each treatment consisted of 4 replicates and 20 one-day old chicks per replication. The evaluation of immune response of chicks was conducted by injecting 5% SRBC suspension, assessment of ileal microflora by removing the ileum contents of the birds and examination of jejunal morphological characteristics by cutting the middle part of the jejunum after slaughter. The results of this study showed that experimental treatments had no significant effect on antibody titer against SRBC. The frequency of heterophils and heterophile to lymphocyte ratio decreased significantly affected by anise and angelica extracts (P<0.05). The total population of aerobic bacteria, Escherichia coli and Coliform bacteria decreased significantly by angelica extract (P<0.05). All treatments containing additive caused significant increase in jejunum villus height when compared to control treatment (P<0.05). The villus height to crypt depth ratio in jejunal was significantly increased by anise and angelica extracts (P<0.05). Generally, the results of this experiment showed that the use of anise and angelica extracts by amount of 200 mg/kg diet may improve immune response, reduce the harmful ileal microflora and improve jejunal morphological characteristics of broiler chickens. | ||
| کلیدواژه‌ها [English] | ||
| Anise extract, Angelica extract, Antibiotic, Probiotic, broilers | ||
| مراجع | ||
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